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1.
Oncogene ; 38(28): 5748, 2019 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-31175341

RESUMEN

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

2.
Oncogene ; 33(30): 3918-26, 2014 Jul 24.
Artículo en Inglés | MEDLINE | ID: mdl-23995782

RESUMEN

We built an in-house oligonucleotide array on which 394 genes were selected based on our Serial Analysis of Gene Expression (SAGE) data and previously reported array data and listed several genes related to cancer progression. Among these, we focused on SEC11A, which encodes the SPC18 protein. SEC11A mRNA expression was measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) in gastric cancer (GC) tissue samples. Expression and distribution of SPC18 protein were investigated by immunohistochemical analysis in two independent GC cohorts (Hiroshima cohort, n=99 and Chiba cohort, n=989). To determine the effect of SPC18 on cell viability and invasiveness in vitro, MTT and Boyden chamber invasion assays were performed. To evaluate the influence of SPC18 on cell growth in vivo, GC cells were injected into severe combined immunodeficiency mice. Levels of TGF-α and EGF in media from the GC cells were measured by enzyme-linked immunosorbent assay (ELISA). Studies in human tissue revealed overexpression of SEC11A mRNA in 40% of 42 GC samples by qRT-PCR. Immunohistochemical analysis of SPC18 revealed that 26 and 20% of GC cases were SPC18-positive in the Hiroshima and Chiba cohorts, respectively. In both cohorts, the Kaplan-Meier analysis showed poorer survival in SPC18-positive GC cases than in SPC18-negative GC cases. Forced expression of SPC18 activates GC cell growth in vitro and in vivo. The levels of TGF-α in culture media from GC cells were reduced by knockdown of SPC18. These results indicate that SPC18 contributes to malignant progression through promotion of TGF-α secretion in GC.


Asunto(s)
Péptido Hidrolasas/metabolismo , Neoplasias Gástricas/metabolismo , Factor de Crecimiento Transformador alfa/metabolismo , Anciano , Animales , Línea Celular Tumoral , Proliferación Celular , Progresión de la Enfermedad , Femenino , Humanos , Estimación de Kaplan-Meier , Masculino , Ratones , Ratones SCID , Análisis Multivariante , Trasplante de Neoplasias , Péptido Hidrolasas/genética , Modelos de Riesgos Proporcionales , Neoplasias Gástricas/mortalidad , Neoplasias Gástricas/patología , Transcriptoma , Carga Tumoral
3.
Phys Rev Lett ; 88(18): 185504, 2002 May 06.
Artículo en Inglés | MEDLINE | ID: mdl-12005696

RESUMEN

We present a new method for imaging surface phonon focusing and dispersion at frequencies up to 1 GHz that makes use of ultrafast optical excitation and detection. Animations of coherent surface phonon wave packets emanating from a point source on isotropic and anisotropic solids are obtained with micron lateral resolution. We resolve rounded-square shaped wave fronts on the (100) plane of LiF and discover isolated pockets of pseudosurface wave propagation with exceptionally high group velocity in the (001) plane of TeO(2). Surface phonon refraction and concentration in a minute gold pyramid is also revealed.

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