RESUMEN
Inspired by the versatility of the direct laser writing carbonization (DLWc) technique as well as the metal-ion-assisted coordination polymer of 3,4,9,10-perylene tetracarboxylic dianhydride (PTCDA), in the present study, we present a DLWc-enabled approach for cost-effectively fabricating a poly-peri-naphthalene (PPN) thin film with the advantages of patternability, environmental benignity, and scalability. Optical and scanning electron microscopy, as well as ultraviolet-visible-near-infrared, Fourier transform infrared, Raman, and X-ray photoelectron spectroscopies, were performed to confirm that the spray-coated thin film of the Mg-PTCDA coordination polymer can be in situ converted into a PPN thin film upon CO2 laser irradiation. The effects of the laser power and Mg2+ concentration on the structure and electrical properties of the laser-processed PPN thin films were investigated. Lastly, we demonstrated that the laser-processed PPN thin films can be used for humidity sensing with characteristics of a rapid response time and excellent hysteresis. It is expected that this new method for fabricating PPN thin films will lead to a wide range of applications of PPN in the fields of sensing, electronics, and energy storage.
RESUMEN
Monocyte-to-macrophage differentiation is tightly controlled in vivo, as disruption of the normal differentiation program can lead to diverse disorders. Caspase-1, the first identified member of the caspase family, regulates differentiation in various cell types such as Th17 cells and adipocytes. However, the contribution of caspase-1 in monocyte-macrophage differentiation remains elusive. Here we report that caspase-1 is significantly downregulated in leukemia cells from patients with acute monocytic leukemia. By using the phorbol 12-myristate 13-acetate-induced cell differentiation model, we found that caspase-1 activation was required for the differentiation of human monocytes to macrophages. Further analysis of peroxisome proliferator-activated receptor γ (PPARγ) protein levels revealed that the monocyte-macrophage differentiation program could be divided into two stages. Caspase-1-mediated downregulation of PPARγ was important in the late stage of monocyte-macrophage differentiation; however, PPARγ protein levels had little effect on the early stage differentiation. Accumulation of PPARγ protein by troglitazone treatment potently suppressed the late stage of macrophage differentiation, which might be linked to inhibition of nuclear factor-κB activity. The data provide a plausible mechanistic basis by which caspase-1 promotes the differentiation of macrophages from monocytes.