Patterns of variations in dorsal colouration of the Italian wall lizard Podarcis siculus.

Research on animal colouration has grown exponentially in the last decade thanks to multidisciplinary approaches. Most studies are focused on trade-offs between communication and mimicry, which represent the two main constraints and drivers of the evolution of body colourations. Reptiles are excellent model species for investigating this field of study and lizards in particular show great variability of body colourations and their functions. We studied the lizard Podarcis siculus, analysing the variations of dorsal colour of three populations and obtained clear patterns of seasonal and ontogenetical variation of dorsal colour. According to baseline colour, males were greener and brighter than females, although no difference in saturation was recorded. According to seasonal variations, analyses showed that both sexes significantly vary in colour over the year: males reached higher peaks of hue and saturation later than females during spring, while females showed higher peaks of brightness and reached earlier similarly to hue and saturation. Ontogenetic variations were recorded only in males, which become greener, less bright and saturated with growing size. Therefore, our results suggest the occurrence of two opposing strategies in colour expression between sexes: males' dorsal colouration plays a major role in communication, while females are more crypsis-oriented.

No evidence of queen immunisation despite transgenerational immunisation in Crematogaster scutellaris ants.

Like vertebrates, invertebrates evolved acquired immunity based on memory-like mechanisms, known as immunisation. Immunisation and its transmission among individuals are phylogenetically ancestral and conserved characters that have been reported in different insect orders. Physiological mechanisms are still largely unknown, and the high variability in responses in different host-parasite systems led to different conclusions. In social insect species, the complex organisation of colonies further complicates the interpretation of the immune responses. In ants, it has been shown that the expression of immunisation depends on species, caste and physiological status of individuals. In this study, we investigate the occurrence of immunisation in queens of Crematogaster scutellaris ants using the fungus Metarhizium anisopliae as elicitor. Foundation in C. scutellaris is claustral and monogynic, allowing us to test the existence of the phenomenon in two distinct physiological conditions, corresponding to the claustral and colonial phases of queens. Queens and foundresses challenged with heavy doses of the pathogen showed higher mortality if previously exposed to light doses, indicating the absence of immunisation in our experimental settings. On the other hand, evidence of the trans-generational immunisation in the same host-parasite system has been recently found, where workers produced by queens exposed to light doses of M. anisopliae survived longer than those belonging to the control group. These results indicate that foundresses exposed to M. anisopliae can elicit an increased resistance in the offspring without providing themselves with a similar increased immune response and that immunisation and trans-generational immunisation are uncoupled phenomena in this host-parasite system.

Trans-generational immunization in the acrobat ant Crematogaster scutellaris.

Trans-generational immunization is defined as the transmission of an enhanced resistance to a pathogen from parents to offspring. By using the host-parasite system of the ant Crematogaster scutellaris and the entomopathogenic fungus Metarhizium anisopliae, we describe this phenomenon for the first time in ants. We exposed four groups of hibernating queens to different treatments (i) a non-lethal dose of live conidiospores in Triton, (ii) a dose of heat-killed conidiospores in Triton, (iii) a control Triton solution, and (iv) a naive control. We exposed their first workers to a high dose of conidiospores and measured mortality rates. Workers produced by queens exposed to live conidiospores survived longer than those belonging to the other groups, while exposure to Triton and dead spores had no effect. Starved workers showed a significantly higher mortality. The treatments did not influence queen mortality, nor the number of offspring they produced at the emergence of the first worker, showing no evidence of immunization costs-at least for these parameters in the first year of colony development. We propose that trans-generational immunization represents an important component of social immunity that could affect colony success, particularly during the critical phase of claustral foundation.