A novel aptasensor for colorimetric monitoring of tobramycin: Strategy of enzyme-like activity of AuNPs controlled by three-way junction DNA pockets.

In this study, a novel colorimetric sensor was introduced to detect tobramycin (TOB) based on controlling the catalytic activity of gold nanoparticles (AuNPs) by the three-way junction aptamer pockets. In the absence of TOB, the surfaces of AuNPs were masked by the three-way junction pockets that prevented their catalytic activation for the reduction of 4-Nitrophenol in the presence of NaBH4. While the formation of the pockets was prevented in the presence of TOB that facilitated the 4-Nitrophenol access to AuNPs. Hence, the catalytic reduction of 4-Nitrophenol induced a color change of the solution from yellow to colorless, highlighting the presence of the target. The aptasensing assay provided good target specificity with a detection limit (LOD) of 1.16 µM and a linear dynamic range over 4-32 µM. The aptasensor was successfully applied to quantitatively monitor TOB in the human serum and milk samples with the LODs of 1.38 and 1.42 µM and recovery values of 94.87-105.75% and 93.75-105.31%, respectively.

Healing acceleration in hamsters of oral mucositis induced by 5-fluorouracil with topical Calendula officinalis.

OBJECTIVE: This study assessed the potential of topical Calendula officinalis extract on the healing of oral mucositis induced by 5-fluorouracil (5-FU) in hamsters. STUDY DESIGN: Oral mucositis was induced in 60 male hamsters by 5-FU (60 mg/kg) on days 0, 5, and 10 of the study. The cheek pouch was scratched with a sterile needle on days 1 and 2. On days 12-17, 5% and 10% C. officinalis gel and gel base groups were treated and then compared with a control group. Macroscopic and microscopic scores and weights were evaluated. RESULTS: Microscopic and macroscopic scores of mucositis were lower in the 5% and 10% C. officinalis gel groups than in the gel base and control groups (P < .05). Weight gain was noted in the treatment groups compared with the gel base and control groups (P < .05). CONCLUSIONS: Calendula officinalis extract accelerated the healing of oral mucositis in hamsters.