Impact of the COVID-19 outbreak on blood supply in two large university hospitals.

OBJECTIVE: This study aimed to examine the relationship between the decrease in elective procedures and the need for blood donation during the novel coronavirus disease (COVID-19) pandemic at university hospitals. BACKGROUND: The COVID-19 pandemic has immensely impacted transfusion medicine. By cancelling elective surgery, the German government hoped to increase the available resources for patients infected with COVID-19, especially in intensive care units, and prevent the shortage of blood products. METHODS/MATERIALS: Over 26 weeks, from the 3rd of February 2020 to the 2nd of August 2020, during the first phase of the pandemic, we assessed the number of crossmatches, blood group typing, use of donated blood, and case mix indices by retrospectively analysing data from two major university hospitals' information systems in Essen and Hamburg, Germany. Data were pooled, analysed, and compared with that of the same period in the previous year. RESULTS: Following the cessation of elective procedures, the number of requests for crossmatches and blood group typing significantly decreased in 2020 compared to that in 2019. However, the number of blood transfusions required was reduced to a lesser extent. The number of outpatient and inpatient cases significantly decreased, whereas the cases requiring transfusion decreased only. CONCLUSION: During the initial phase of the pandemic, transfusion medicine, especially in large institutions, faced an almost unchanged high demand for donated blood. This should be considered regarding personnel and blood donation allocations. Therefore, we developed a monitoring system to display the availability of blood products in real-time. The quick and easy display of in-stock and expiring blood products can optimise the use of this valuable resource.

Pregnancies and Neonatal Outcomes in Patients with Sickle Cell Disease (SCD): Still a (High-)Risk Constellation?

BACKGROUND: This monocentric study conducted at the University Hospital of Essen aims to describe maternal and fetal/neonatal outcomes in sickle cell disease (SCD) documented between 1996 to 2021 (N = 53), reflecting the largest monocentric analysis carried out in Germany. METHODS/RESULTS: 46 pregnancies in 22 patients were followed. None of the patients died. In total, 35% (11/31) of pregnancies were preterm. 15 pregnancies in eight patients were conceived on hydroxycarbamide (HC), of which nine had a successful outcome and three were terminated prematurely. There was no difference regarding the rate of spontaneous abortions in patients receiving HC compared to HC-naive patients prior to conception. In patients other than HbS/C disease, pregnancies were complicated by vaso-occlusive crises (VOCs)/acute pain crises (APCs) (96%, 23/24); acute chest syndrome (ACS) (13%, 3/24), transfusion demand (79%, 19/24), urinary tract infections (UTIs) (42%, 10/24) and thromboembolic events (8%, 2/24). In HbS/C patients complications included: VOCs/APCs (43%, 3/7; ACS: 14%, 1/7), transfusion demand (14%, 1/7), and UTIs (14%, 1/7). Independent of preterm deliveries, a significant difference with respect to neonatal growth in favor of neonates from HbS/C mothers was observed. CONCLUSION: Our data support the results of previous studies, highlighting the high rate of maternal and fetal/neonatal complications in pregnant SCD patients.

SARS-CoV-2-specific humoral and cellular immunity in two renal transplants and two hemodialysis patients treated with convalescent plasma.

When patients with chronic kidney disease are infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) they can face two specific problems: virus-specific immune responses may be impaired and remdesivir, an antiviral drug described to shorten recovery, is contraindicated. Antiviral treatment with convalescent plasma (CP) could be an alternative treatment option. In this case report, we present two kidney transplant recipients and two hemodialysis patients who were infected with SARS-CoV-2 and received CP. Antibodies against the receptor-binding domain in the S1 subunit of the SARS-CoV-2 spike protein were determined sequentially by immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) and neutralization assay and specific cellular responses by interferon-gamma ELISpot. Before treatment, in both kidney transplant recipients and one hemodialysis patient antibodies were undetectable by ELISA (ratio < 1.1), corresponding to low neutralizing antibody titers (≤1:40). ELISpot responses in the four patients were either weak or absent. After CP treatment, we observed an increase of SARS-CoV-2-specific antibodies (IgG ratio and neutralization titer) and of specific cellular responses. After intermittent clinical improvement, one kidney transplant recipient again developed typical symptoms on Day 12 after treatment and received a second cycle of CP treatment. Altogether, three patients clinically improved and could be discharged from the hospital. However, one 83-year-old multimorbid patient deceased. Our data suggest that the success of CP therapy may only be temporary in patients with chronic kidney disease; which requires close monitoring of viral load and antiviral immunity and possibly an adaptation of the treatment regimen.

Cellular Immunity in COVID-19 Convalescents with PCR-Confirmed Infection but with Undetectable SARS-CoV-2-Specific IgG.

We investigated immune responses against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) among a group of convalescent, potential blood donors in Germany who had PCR-confirmed SARS-CoV-2 infection. Sixty days after onset of symptoms, 13/78 (17%) study participants had borderline or negative results to an ELISA detecting IgG against the S1 protein of SARS-CoV-2. We analyzed participants with PCR-confirmed infection who had strong antibody responses (ratio >3) as positive controls and participants without symptoms of SARS-CoV-2 infection and without household contact with infected patients as negative controls. Using interferon-γ ELISpot, we observed that 78% of PCR-positive volunteers with undetectable antibodies showed T cell immunity against SARS-CoV-2. We observed a similar frequency (80%) of T-cell immunity in convalescent donors with strong antibody responses but did not detect immunity in negative controls. We concluded that, in convalescent patients with undetectable SARS-CoV-2 IgG, immunity may be mediated through T cells.

Invasive Mold Infection of the Central Nervous System in Immunocompromised Children.

Background: Due to the difficulties in the definite diagnosis, data on brain imaging in pediatric patients with central nervous system (CNS)-invasive mold infection (IMD) are scarce. Our aim was to describe brain imaging abnormalities seen in immunocompromised children with CNS-IMD, and to analyze retrospectively whether specific imaging findings and sequences have a prognostic value. Methods: In a retrospective study of 19 pediatric patients with proven or probable CNS-IMD, magnetic resonance imaging (MRI)-findings were described and analyzed. The results were correlated with outcome, namely death, severe sequelae, or no neurological sequelae. Results: 11 children and 8 adolescents (11/8 with proven/probable CNS-IMD) were included. Seven of the patients died and 12/19 children survived (63%): seven without major neurological sequelae and five with major neurological sequelae. Multifocal ring enhancement and diffusion restriction were the most common brain MRI changes. Diffusion restriction was mostly seen at the core of the lesion. No patient with disease limited to one lobe died. Perivascular microbleeding seen on susceptibility weighted imaging (SWI) and/or gradient-echo/T2* images, as well as infarction, were associated with poor prognosis. Conclusions: The presence of infarction was related to poor outcome. As early microbleeding seems to be associated with poor prognosis, we suggest including SWI in routine diagnostic evaluation of immunocompromised children with suspected CNS-IMD.

Galactomannan and PCR in the Central Nervous System to Detect Invasive Mold Disease - A Retrospective Analysis in Immunocompromised Children.

Invasive mold disease (IMD) of the central nervous system (CNS) is a severe infectious complication in immunocompromised patients, but early microbiological diagnosis is difficult. As data on the value of biomarkers in the CNS are scarce, in particular in children, we retrospectively analyzed the performance of galactomannan (GM) and PCR assays in CNS samples of 15 children with proven and probable CNS IMD and of 32 immunocompromised children without fungal infection. Galactomannan in the cerebrospinal fluid (CSF) was assessed in nine of the 15 pediatric patients and was positive in five of them. Polymerase chain reaction (PCR) was performed in eight of the 15 patients and detected nucleic acids from molds in six patients. Galactomannan and PCR in CNS samples were the only positive microbiologic parameter in the CNS in three and two patients, respectively. In four patients, PCR specified the pathogen detected in microscopy. Galactomannan and PCR results remained negative in the CSF of all immunocompromised children without evidence for CNS IMD. Our data suggest that GM and PCR in CNS specimens are valuable additional tools in diagnosing CNS IMD and should be included in the work up of all pediatric patients with suspected mold disease of the CNS.

Invasive mold disease of the central nervous system in children and adolescents with cancer or undergoing hematopoietic stem cell transplantation: Analysis of 29 contemporary patients.

BACKGROUND: Invasive mold disease (IMD) is a severe infectious complication in immunocompromised patients. The outcome of central nervous system (CNS) IMD is poor, but contemporary data, in particular in the pediatric setting, are lacking. PROCEDURE: For this retrospective multicenter analysis, pediatric patients < 18 years with proven or probable CNS IMD receiving chemotherapy or undergoing allogeneic HSCT were reported by the local investigator. CNS IMD had to be diagnosed between 2007 and 2016. Proven CNS IMD was defined as compatible CNS imaging or macroscopic autopsy findings in conjunction with a positive microscopic or microbiological result in the brain tissue or cerebrospinal fluid. Probable CNS IMD was defined as compatible CNS imaging findings in combination with proven or probable IMD at a site outside the CNS. RESULTS AND CONCLUSIONS: A total of 29 patients (median age, 14 years; 14 allogeneic HSCT recipients) were diagnosed with proven (n = 12) or probable (n = 17) CNS IMD. Aspergillus spp. was the most common fungal pathogen. All but one patient had IMD sites outside the CNS and eight patients (27.6%) were neurologically asymptomatic at diagnosis of CNS IMD. Forty-nine percent of the patients survived CNS IMD; however, 46.7% of the survivors suffered from severe long-term neurological sequelae. Our data suggest that (1) outcome of CNS IMD has improved in children as compared with previous series, (2) half of surviving patients suffer from severe neurological sequelae, and (3) imaging of the CNS should be performed in all children with IMD irrespective of neurological symptoms.

[Recommendations for Diagnostics and Therapy of Children with Cancer Presenting with Fever and Neutropenia - Comparison of Two Current Guidelines]. / Empfehlungen zur Diagnostik und Therapie bei krebskranken Kindern mit Fieber und Neutropenie ­ Vergleich zweier aktueller Leitlinien.

Immunocompromised children and adolescents receiving treatment for cancer have a considerably increased risk for infection. Neutropenia is the most important single risk factor for infectious complications, and fever in neutropenia is considered as an emergency. Whereas guidelines for the management of fever in neutropenic adults have been established for decades, specific pediatric guidelines have not been developed until recently. As children differ in many aspects from adults such as in the underlying malignancy or in the availability and dosing of antimicrobial compounds, guidelines for pediatric patients are important. This article reviews similarities and differences between the recently published German interdisciplinary guideline of the German Societies of Pediatric Infectious Diseases and Pediatric Oncology and Hematology and a guideline developed by a panel of international experts for the management of fever in neutropenia in children and adolescents.

Polyfluorinated compounds in the atmosphere of the Atlantic and Southern Oceans: evidence for a global distribution.

High volume air samples taken onboard several research vessels in the Atlantic Ocean, the Southern Ocean, and the Baltic Sea as well as at one land-based site close to Hamburg, Germany, in 2007 and 2008 were analyzed for per- and polyfluorinated organic compounds (PFCs). A set of neutral, volatile PFCs such as fluorotelomer alcohols (FTOH) or perfluoroalkyl sulfonamides and ionic nonvolatile PFCs like perfluorinated carboxylates (PFCA) and sulfonates (PFSA) were collected on PUF/XAD-2/PUF cartridges and glass fiber filters and determined using GC-MS and HPLC-MS/MS. PFCs were detected in all air samples, even in Antarctic regions, and occurred predominantly in the gas phase. Total gas-phase concentrations of ship-based samples ranged from 4.5 pg m(-3) in the Southern Ocean to 335 pg m(-3) in European source regions. Concentrations of 8:2 FTOH, the analyte that was usually observed in highest concentrations, were between 1.8 and 130 pg m(-3). PFC concentrations decreased from continental regions toward marine regions and from Central Europe toward the Arctic and Antarctica. Southern hemispheric concentrations of individual PFCs were significantly lower than those of the northern hemisphere. On the basis of this data set, marine background PFC concentrations and atmospheric residence times were calculated. This study gives further evidence that volatile PFCs undergo atmospheric long-range transportto remote regions and may contribute to their contamination with persistent PFCA and PFSA.

Organochlorine pesticides and PAHs in the surface water and atmosphere of the North Atlantic and Arctic Ocean.

Surface seawater and boundary layer atmospheric samples were collected on the FS Polarstern during cruise ARKXX in the North Atlantic and Arctic Ocean in 2004. Samples were analyzed for persistent organic pollutants (POPs), with a focus on organochlorine pesticides, including hexachlorocyclohexanes (HCHs), chlordanes, DDTs, hexachlorobenzene (HCB), and polycyclic aromatic hydrocarbons. In addition, the enantiomer fractions (EFs) of pesticides, notably alpha-HCH and cis-chlordane (CC), were determined. Concentrations of dissolved HCB increased from near Europe (approximately 1-2 pg/L) toward the high Arctic (4-10 pg/L). For dissolved HCB, strongest correlations were obtained with the average air or water temperature during sampling, not latitude. In the western Arctic Ocean, surface waters with elevated concentrations of HCB (5-10 pg/ L) were flowing out of the Arctic Ocean as part of the East Greenland current In contrast to dissolved compounds, atmospheric POPs did not display trends with temperature. Air-water exchange gradients suggested net deposition for all compounds, though HCB was closest to air-water equilibrium. EFs for alpha-HCH in seawater ranged from 0.43 to 0.50, except for two samples from 75 degrees N in the East Greenland Sea, with EFs of 0.31 and 0.37. Lowest EF (0.47) for CC were also at 75 degrees N, other samples had EFs from 0.49 to 0.52. It is suggested that samples from around 75 degrees N in the Greenland Gyre represented a combination of surface and older/deeper Arctic water.

Annual time series of air concentrations of polyfluorinated compounds.

Per- and polyfluorinated organic compounds (PFC) in air were determined in samples taken at two sites in the vicinity of Hamburg, Germany, over a period of 14 months. PUF/XAD-2/PUF cartridges and glass fiber filters were applied for the collection of airborne PFC. A set of volatile, neutral PFCs such as fluorotelomer alcohols (FTOH) or perfluorinated sulfonamides and ionic, nonvolatile PFC like perfluorinated carboxylates and sulfonates were determined using GC-MS and HPLC-MS/MS. Backward trajectory analysis was performed to elucidate the origin of the air mass parcels sampled. PFCs were predominantly detected in the gas phase. A fluctuating baseline presenting north German background levels and singular events of high concentrations were characteristic for the time series of all analytes and both locations. The origin of sampled air was the driving parameter influencing the PFC levels. Elevated PFC concentrations occurred in air arriving from industrialized and populated regions west and southwest of Hamburg. Maximum individual PFC concentrations reached 600 pg m(-3) (8:2 FTOH) in the gas phase and 13 pg m(-3) (perfluorooctane sulfonate) in the particle phase. The class of FTOH clearly dominated the gas-phase substance spectrum. The compound that was detected in highest concentrations was 8:2 FTOH. Individual gas-phase PFC concentrations were higher in summer than in winter. Temperature-dependent emissions of volatile and semi-volatile PFCs from diffuse sources to the gas phase are presumed to be responsible for this observation.

Concentrations of polybrominated diphenyl ethers (PBDEs) in matched samples of human milk, dust and indoor air.

Polybrominated diphenyl ethers (PBDEs) are lipophilic, persistent pollutants found worldwide in environmental and human samples. Exposure pathways for PBDEs remain unclear but may include food, air and dust. The aim of this study was to conduct an integrated assessment of PBDE exposure and human body burden using 10 matched samples of human milk, indoor air and dust collected in 2007-2008 in Brisbane, Australia. In addition, temporal analysis was investigated comparing the results of the current study with PBDE concentrations in human milk collected in 2002-2003 from the same region. PBDEs were detected in all matrices and the median concentrations of BDEs -47 and -209 in human milk, air and dust were: 4.2 and 0.3 ng/g lipid; 25 and 7.8 pg/m(3); and 56 and 291 ng/g dust, respectively. Significant correlations were observed between the concentrations of BDE-99 in air and human milk (r=0.661, p=0.038) and BDE-153 in dust and BDE-183 in human milk (r=0.697, p=0.025). These correlations do not suggest causal relationships - there is no hypothesis that can be offered to explain why BDE-153 in dust and BDE-183 in milk are correlated. The fact that so few correlations were found in the data could be a function of the small sample size, or because additional factors, such as sources of exposure not considered or measured in the study, might be important in explaining exposure to PBDEs. There was a slight decrease in PBDE concentrations from 2002-2003 to 2007-2008 but this may be due to sampling and analytical differences. Overall, average PBDE concentrations from these individual samples were similar to results from pooled human milk collected in Brisbane in 2002-2003 indicating that pooling may be an efficient, cost-effective strategy of assessing PBDE concentrations on a population basis. The results of this study were used to estimate an infant's daily PBDE intake via inhalation, dust ingestion and human milk consumption. Differences in PBDE intake of individual congeners from the different matrices were observed. Specifically, as the level of bromination increased, the contribution of PBDE intake decreased via human milk and increased via dust. As the impacts of the ban of the lower brominated (penta- and octa-BDE) products become evident, an increased use of the higher brominated deca-BDE product may result in dust making a greater contribution to infant exposure than it does currently. To better understand human body burden, further research is required into the sources and exposure pathways of PBDEs and metabolic differences influencing an individual's response to exposure. In addition, temporal trend analysis is necessary with continued monitoring of PBDEs in the human population as well as in the suggested exposure matrices of food, dust and air.

Polychlorinated biphenyls (PCBs) in air and seawater of the Atlantic Ocean: sources, trends and processes.

Air and seawater samples were collected on board the RV Polarstern during a cruise from Bremerhaven, Germany to Cape Town, South Africa from October-November 2005. Broad latitudinal trends were observed with the lowest sigma27PCB air concentration (approximately 10 pg m(-3)) in the South Atlantic and the highest (approximately 1000 pg m(-3)) off the west coast of Africa. Sigma(ICES)PCBs ranged from 3.7 to 220 pg m(-3) in air samples and from 0.071 to 1.7 pg L(-1) in the dissolved phase seawater samples. Comparison with other data from cruises in the Atlantic Ocean since 1990 indicate little change in air concentrations over the remote open ocean. The relationship of gas-phase partial pressure with temperature was examined using the Clausius-Clapeyron equation; significant temperature dependencies were found for all PCBs over the South Atlantic, indicative of close air-water coupling. There was no temperature dependence for atmospheric PCBs overthe North Atlantic, where concentrations were controlled by advection of contaminated air masses. Due to large uncertainties in the Henry's Law Constant (HLC), fugacity fractions and air-water exchange fluxes were estimated using different HLCs reported in the literature. These suggest that conditions are close to air-water equilibrium for most of the ocean, but net deposition is dominating over volatilization in parts of the transect. Generally, the tri- and tetrachlorinated homologues dominated the total flux (> 70%). Total PCB fluxes (28, 52, 118, 138, and 153) ranged from -7 to 0.02 ng m(-2) day(-1).

PAHs in air and seawater along a North-South Atlantic transect: trends, processes and possible sources.

Polycyclic aromatic hydrocarbons (PAHs) were simultaneously measured in air and surface seawater between 49 degrees N and 25 degrees S in the open Atlantic Ocean. Elevated concentrations of PAHs (sigma10 PAHs approximately 1.4-2.5 ng m(-3) air, and 0.7-1 ng L(-1) seawater) occurred in the Biscay Bay and off the northwest coast of Africa. The unexpectedly high concentrations off NW Africa were discussed assessing the possible contribution of the emerging oil industry along the African shore, the role of biomass burning and natural sources of PAHs. In the southern Atlantic, concentrations of PAHs were close to detection limits (sigma10 PAHs approximately 0.02-0.5 ng m(-3) air, and 0.06-0.5 ng L(-1) seawater) and showed decreasing trends with increasing latitudes. Correlations of PAHs' partial pressures versus inverse temperature were not significant in contrast to results for polychlorinated biphenyls from the same transect. This could have been due to the importance of ongoing primary sources and the shorter atmospheric life-times of PAHs. Air-water fugacity ratios (fa/fw) were calculated for selected compounds. They were close to 1 for fluoranthene and pyrene in remote open ocean areas suggesting air-water partitioning near equilibrium. Ratios for anthracene and phenanthrene were < 0.3 in the remote tropical Atlantic, suggesting net volatilization.

Optimized method avoiding solvent-induced response enhancement in the analysis of volatile and semi-volatile polyfluorinated alkylated compounds using gas chromatography-mass spectrometry.

A method for the analysis of airborne (semi-)volatile polyfluorinated alkyl substances (PFASs) was optimized to avoid solvent-induced response enhancements as observed using ethyl acetate as extraction solvent (recoveries up to 300%, likely due to acetic acid as impurity). Of nine solvents tested, only the use of acetone, dichloromethane, methyl tert-butyl ether, and acetone:petroleum ether (1:1, v/v) resulted in recoveries below 100% and acetone:methyl tert-butyl ether (1:1, v/v) was chosen as suited extraction solvent. An appropriate GC capillary column and the application of mass-labelled internal standards appeared to be essential for these analyses. Instrumental limits of detection of <0.2 pg (dimethylperfluoroocatanesulfonamide, ethyl-[(2)H(5)]perfluorooctanesulfonamide, methyl-[(2)H(3)]perfluorooctanesulfonamide) to 8.2 pg (perfluorooctanesulfonamide) and LOQ of 0.4 pg (dimethylperfluoroocatanesulfonamide, ethyl-[(2)H(5)]perfluorooctanesulfonamide, methyl-[(2)H(3)]perfluorooctanesulfonamide) to 16.4 pg (perfluorooctanesulfonamide) were determined.

Air-sea exchange fluxes of synthetic polycyclic musks in the North Sea and the Arctic.

Synthetic polycyclic musk fragrances Galaxolide (HHCB) and Tonalide (AHTN) were measured simultaneously in air and seawater in the Arctic and the North Sea and in the rural air of northern Germany. Median concentrations of gas-phase HHCB and AHTN were 4 and 18 pg m(-3) in the Arctic, 28 and 18 pg m(-3) in the North Sea, and 71 and 21 pg m(-3) in northern Germany, respectively. Various ratios of HHCB/AHTN implied that HHCB is quickly removed by atmospheric degradation, while AHTN is relatively persistent in the atmosphere. Dissolved concentrations ranged from 12 to 2030 pg L(-1) for HHCB and from below the method detection limit (3 pg L(-1)) to 965 pg L(-1) for AHTN with median values of 59 and 23 pg L(-1), respectively. The medians of volatilization fluxes for HHCB and AHTN were 27.2 and 14.2 ng m(-2) day(-1) and the depositional fluxes were 5.9 and 3.3 ng m(-2) day(-1), respectively, indicating water-to-air volatilization is a significant process to eliminate HHCB and AHTN from the North Sea. In the Arctic, deposition fluxes dominated the air-sea gas exchange of HHCB and AHTN, suggesting atmospheric input controls the levels of HHCB and AHTN in the polar region.

Occurrence and air-sea exchange of phthalates in the Arctic.

Air and seawater samples were taken simultaneously to investigate the distribution and air-sea gas exchange of phthalates in the Arctic onboard the German Research Ship FS Polarstern. Samples were collected on expeditions ARK XX1&2 from the North Sea to the high Arctic (60 degrees N-85 degrees N) in the summer of 2004. The concentration of sigma6 phthalates (dimethyl phthalate (DMP), diethyl phthalate (DEP), di-i-butyl phthalate (DiBP), di-n-butyl phthalate (DnBP), butylbenzyl phthalate (BBP), and diethylhexyl phthalate (DEHP)) ranged from 30 to 5030 pg L(-1) in the aqueous dissolved phase and from 1110 to 3090 pg m(-3) in the atmospheric gas phase. A decreasing latitudinal trend was present in the seawater and to a lesser degree in the atmosphere from the Norwegian coast to the high Arctic. Overall, deposition dominated the air-sea gas exchange for DEHP, while volatilization from seawater took place in the near-coast environment. The estimated net gas deposition of DEHP was 5, 30, and 190 t year(-1) for the Norwegian Sea, the Greenland Sea, and the Arctic, respectively. This suggests that atmospheric transport and deposition of phthalates is a significant process for their occurrence in the remote Atlantic and Arctic Ocean.

Development and application of a simplified sampling method for volatile polyfluorinated alkyl substances in indoor and environmental air.

A new, fast and simple sampling method using commercially available Isolute ENV+ solid-phase extraction (SPE) cartridges for the enrichment of neutral, volatile polyfluorinated alkyl substances (PFAS) was developed and applied to selected air samples. The SPE cartridges showed good retention capacity for the target analytes, and most of the investigated compounds could be quantified in 20m(3) indoor air. Employing the developed method, it was shown that high levels of selected fluorotelomer alcohols (FTOHs) and N-alkyl fluorooctane sulfonamides/-ethanols (FOSAs/FOSEs) evaporated from a paraglider. Furthermore, the new method was compared to the 'classical' approach using glass-fibre filters (GFFs) and XAD-2 resin sandwiched between polyurethane foam plugs (PUF/XAD/PUF) to investigate environmental air concentrations in metropolitan Hamburg. Due to the high counter pressure of SPE cartridges, only low-volume air sampling was feasible. Therefore, the trace levels of FOSAs/FOSEs occurring in environmental air could only be quantified occasionally in the samples enriched on SPE cartridges. However, quantitative analysis of the higher concentrated 6:2 FTOH, 8:2 FTOH and 10:2 FTOH was possible in all low-volume environmental air samples. Finally, the determination of ionic PFAS, including perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA), bound to airborne particles in the air samples from Hamburg is described.

Analysis of per- and polyfluorinated alkyl substances in air samples from Northwest Europe.

Air samples were collected from 4 field sites in Europe: 2 sites from the UK, Hazelrigg (semi-rural) and Manchester (urban); 1 site from Ireland: Mace Head (rural); and 1 site from Norway: Kjeller (rural). Additionally, air samples were taken from indoor locations in Tromsø, Norway. Air samples were collected using high-volume air samplers employing sampling modules containing glass-fibre filters (GFFs, particle phase), and glass columns with a polyurethane foam (PUF)-XAD-2-PUF sandwich (gaseous phase). Typical outdoor air volumes required for the determination of per- and polyfluorinated alkyl substances (PFAS) ranged from 500-1800 m3. GFFs and PUF-XAD columns were analysed separately to obtain information on phase partitioning. All air samples were analysed for volatile, neutral PFAS, with selected GFF samples halved for analysis of both neutral and airborne particle-bound ionic PFAS. Volatile PFAS were extracted from air samples by cold-column immersion with ethyl acetate, and were analysed by gas chromatography-mass spectrometry in the positive chemical ionisation mode (GC-PCI-MS). Ionic PFAS were extracted from GFFs by sonication in methanol, and were analysed by liquid chromatography-time-of-flight-mass spectrometry (LC-TOF-MS) using electrospray ionisation in the negative ion mode (ESI-). Perfluorooctanoate (PFOA) was often the predominant analyte found in the particulate phase at concentrations ranging from 1-818 pg m(-3), and 8:2 fluorotelomer alcohol (FTOH) and 6:2 FTOH were the prevailing analytes found in the gas phase, at 5-243 pg m(-3) and 5-189 pg m(-3), respectively. These three PFAS were ubiquitous in air samples. Many other PFAS, both neutral and ionic, were also present, and levels of individual analytes were in the 1-125 pg m(-3) range. Levels of some PFAS exceeded those of traditional persistent organic pollutants (POPs). In this study, the presence of 12:2 FTOH and fluorotelomer olefins (FTolefins), and ionic PFAS other than perfluorooctane sulfonate (PFOS) and PFOA, are reported in air samples for the first time. Concentrations of neutral PFAS were several orders of magnitude higher in indoor air than outdoor air, making homes a likely important diffuse source of PFAS to the atmosphere. Our repeated findings of non-volatile ionic PFAS in air samples raises the possibility that they might directly undergo significant atmospheric transport on particles away from source regions, and more atmospheric measurements of ionic PFAS are strongly recommended.

Latitudinal gradient of airborne polyfluorinated alkyl substances in the marine atmosphere between Germany and South Africa (53 degrees N-33 degrees S).

Neutral, volatile polyfluorinated alkyl substances (PFAS) were determined in high-volume air samples collected onboard the German research vessel Polarstern during cruise ANTXXIII-1 between Bremerhaven, Germany (53 degrees N) and Capetown, Republic of South Africa (33 degrees S) in fall 2005. An optimized and validated analytical protocol was used for the determination of several fluorotelomer alcohols (FTOHs) as well as N-alkylated fluorooctane sulfonamides and sulfonamidoethanols (FOSAs/FOSEs). Quantitative analyses were done by gas chromatography-mass spectrometry. This study provides the first concentration data of airborne PFAS from the Southern Hemisphere. Results indicate a strongly decreasing concentration gradient from the European continent toward less industrialized regions. The study confirms that airborne PFAS are mainly restricted to the Northern Hemisphere with a maximum concentration of 190 pg/m3 (8:2 FTOH) in the first sample collected in the channel between the European mainland and the UK. However, south of the equator, trace amounts of several FTOHs and FOSAs with a maximum of 14 pg/m3 (8:2 FTOH) could still be detected. Furthermore, a selection of ionic PFAS including perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) were determined in the particulate phase of high-volume air samples by liquid chromatography-mass spectrometry. Levels of ionic PFAS were almost 2 orders of magnitude lower than those of neutral PFAS, with maximum concentrations in the first sample of 2.5 pg/m3 (PFOS) and 2.0 pg/m3 (PFOA).