An allometric model-based approach for estimating biomass in seven Indian bamboo species in western Himalayan foothills, India.

The rapid growth rate, high biomass production, and annual harvesting make bamboo a suitable species for commercial production. Allometric equations for many broadleaf and conifer tree species are available. However, knowledge of biomass production and allometric equations of bamboos is limited. This study aims to develop species- specific allometric models for predicting biomass and synthetic height values as a proxy variable for seven bamboo species in Himalayan foothills. Two power form-based allometric models were used to predict aboveground and culm biomass using diameter at breast height (D) alone and D combined with culm height (H) as an independent variable. This study also extended to establishing an H-D allometric model that can be used to generate synthetic H values as a proxy to missing H. In the seven bamboo species studied, among three major biomass components (culm, branch and foliage), culm is the most important component with the highest share (69.56-78.71%). The distribution of percentage (%) share of culm, branch and foliage to above-ground fresh weight varies significantly between different bamboo species. D. hamiltonii has the highest productivity for above-ground biomass components. Ratio of dry to fresh weight of seven bamboo species was estimated for culm, branch, foliage and above-ground biomass to convert fresh weight to dry weight.

Build-up of labile, non-labile carbon fractions under fourteen-year-old bamboo plantations in the Himalayan foothills.

Carbon fractions under different bamboo species viz., Bambusa balcooa, Bambusa bambos, Bambusa nutans, Dendrocalamus hamiltonii, Dendrocalamus asper and Dendrocalamus strictus were evaluated to understand the potential of these different bamboo species in soil rehabilitation in Himalayan foothills. The highest accumulation of the different carbon fractions likes very labile (6.12 mg g-1), less labile (2.55 mg g-1) and non-labile (11.40 mg g-1) was observed under D. hamiltonii, while highest labile fraction (3.17 mg g-1) was recorded under D. strictus. The highest active (8.85 mg g-1) and passive pool (13.95 mg g-1) were recorded under D. hamiltonii. Higher carbon management index (CMI) was obtained under D. hamiltonii (186.04) which was comparable with D. strictus (182.66) and B. nutans (179.24). Among all the six species, D. hamiltonii had the highest buildup of active and passive pool in both the soil depths. Bamboo plantations irrespective of the different species helped in enhancing the SOC fraction and enhanced C buildup in the soil in comparison to the open fallow land and holds potential in combating the problems of land degradation and soil rehabilitation.

Lignolytic mushroom Lenzites elegans WDP2: Laccase production, characterization, and bioremediation of synthetic dyes.

A mycoremedial study was undertaken for decolourization of synthetic dyes using wood rot fungal culture Lenzites elegans WDP2. The culture was isolated from decaying wood as fruiting body, and identified on the basis of 5.8S ITS rRNA gene sequence analysis. Qualitative plate screening of culture showed extracellular laccase and lignin peroxidase production, while only laccase enzyme was produced in higher amount (156.793 Uml-1) in minimal salt broth medium containing glucose and veratryl alcohol. Laccase activity was increased up to 189.25 Uml-1 after optimization of laccase production by optimization of one variable at a time approach. Molecular characterization of laccase enzyme was done using SDS PAGE and Native PAGE based isozyme analyses. The culture was able to decolorize three synthetic dying compounds (congo red, Malachite green and brilliant green) in broth media, while showed very less decolourization in plate assay. The fungal culture varied in their dye decolourizing potential in broth culture, showing 92.77%, 21.27% and 98.8% maximum decolourization of brilliant green, malachite green and congo red respectively. The congo red dye was completely bio-absorbed by fungal culture within one month. The fungal decolourized broth also revealed the extracellular laccase activity; varied from 10 Uml-1 to 68.5 Uml-1 in all the three cases, supports the involvement of laccase enzyme in decolorization. Phase contrast microscopy clearly revealed bio-sorption of the dyes by fungal culture into the mycelium/spores in the photomicrographs.

Biochemical Characterization of Fungus Isolated during In vitro Propagation of Bambusa balcooa.

BACKGROUND: Bambusa balcooa (Poaceae: Bambusoideae) is a multipurpose bamboo species, which is native of the Indian subcontinent. B. balcooa is regarded as one of the best species for scaffolding and building purposes because of its strong culm. Other uses include paper pulp, handicrafts, and products of the wood chip industry. Due to these various uses in industries, this species has been identified as one of the priority bamboos by the National Bamboo Mission. OBJECTIVE: This study is designed to analyze the identification of fungus and develop the strategy to eliminate the contamination during in vitro establishment of B. balcooa through nodal part. Fungus contamination is a problem which is encountered during in vitro establishment of B. balcooa cultures. MATERIALS AND METHODS: In the present study, fungus contamination from in vitro cultured plant has been isolated and subjected to partial sequence analysis of the 18S rRNA gene to identify the fungus strain. Experiments were designed to develop a strategy for removal of the fungus contamination with the help of antifungal compounds and commercial antimicrobial supplement supplied by HiMedia. RESULTS: Fusarium equiseti was identified as endophytic fungus. It was observed that antimicrobial supplement at concentration of 500 µl/l was more effective concentration to remove fungus contamination and not showed any detrimental effect on growth parameters of shoot. CONCLUSION: This experiment would help in identification and to get rid of fungal contamination and improve the in vitro establishment of B. balcooa cultures for large-scale propagation. SUMMARY: Endogenous fungus was isolated from contaminated culture of B. balcooa, and it was identified as Fusarium equiseti and submitted to NCBI under accession no. KP274872. The endophytic fungus had shown substantial production of amylase, cellulase, and protease media. Gibberellic acid (GA3) production by F. equiseti was maximum on the 7th day on inoculation. Abbreviations used:B. balcooa: Bambusa balcooa, F. equiseti: Fusarium equiseti, PDA: Potato dextrose agar, PCR: Polymerase chain reaction, MS: Murashige and Skoog's, BAP: 6-Benzylaminopurine, ITS1/4: Internal transcribed spacer region 1/4, GA3: Gibberellic acid.