Genes involved in the Type I pheromone biosynthesis pathway and chemoreception from the sex pheromone gland transcriptome of Dioryctria abietella.

Dioryctria abietella is a coniferous seed orchard pest that can damage a series of host plants and cause huge losses to the forest economy. Sex pheromones play an important role in lepidopteran sex communication for reproduction and can be used as biological control agents to monitor and trap pests. However, the genes involved in the biosynthesis, transportation, and degradation of D. abietella sex pheromones have not been studied extensively. Transcriptome analysis of female D. abietella sex pheromone glands (PGs) revealed that 210 candidate genes might be involved in sex pheromone biosynthesis (139 genes) and chemoreception systems (71 genes). The gene expression patterns exhibited four desaturase genes (DabiDES4-7) and one fatty acid reductase gene (DabiFAR6), which were more highly expressed in sex pheromone glands than in other tissues, suggesting that these enzymes play an important role in D. abietella sex pheromone synthesis. In addition, most DabiOBPs showed high expression in antennae, but only DabiOBP4 exhibited specific expression in sex pheromone glands, suggesting that they may play many physiological roles in D. abietella. We put forth a reasonable hypothesis about type I pheromone biosynthesis pathways based on these genes identified in the D. abietella sex pheromone gland transcriptome. Our findings lay a foundation for population monitoring, mating disruption, mass trapping, and the development of ecologically acceptable management strategies.

Identification and analysis of olfactory genes in Dioryctria abietella based on the antennal transcriptome.

The coneworm Dioryctria abietella (Lepidoptera: Pyralidae) is an economy devastating pest that infests many valuable conifer species in the Holarctic regions, such as Pinus koraiensis Siebold and Zucc. The chemosensory system plays a crucial role in the mating, foraging, and ovipositing of this pest, and therefore it is desirable to identify chemosensory molecules for pest control. However, little is known at molecular level about the olfactory mechanisms in D. abietella. In the present study, we first established antennal transcriptomes of D. abietella and identified 132 putative chemosensory genes, including 15 odorant-binding proteins, 18 chemosensory proteins, 65 odorant receptors, 5 sensory neuron membrane proteins, 24 ionotropic receptors, and 5 gustatory receptors. In addition, phylogenetic trees were constructed for chemosensory genes to investigate the orthologs between D. abietella and other species of insects. Furthermore, we also compared the patterns of motifs between OBPs and CSPs using MEME. Additionally, we observed that most of DabiOBPs and DabiCSPs had the antenna-biased expression by quantitative real-time PCR (RT-qPCR), and there was a higher expression of DabiPBP1 and DabiPBP2 in male antennae than in female antennae. The binding sites of DabiPBPs (DabiPBP1, DabiPBP2) and DabiPRs (DabiOR19, DabiOR31) to the sex pheromone were predicted well by three-dimensional docking structure modelling and molecular docking. Our finding supplied a foundation for further research on the binding process of OBPs or CSPs and sensing process of ORs, SNMPs, IRs or GRs in D. abietella.