Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 6 de 6
Filtrar
Más filtros











Base de datos
Intervalo de año de publicación
1.
Blood ; 83(4): 931-8, 1994 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-8111064

RESUMEN

About 80% to 90% of females are informative for X-inactivation/methylation analysis with the probe M27 beta, which would therefore seem attractive in assessing clonality in hematologic cell populations. Eighteen acute lymphoid or myeloid leukemias, three chronic lymphocytic leukemias, and three chronic myelogenous leukemias as well as 12 malignant non-Hodgkin's lymphomas mostly showed extremely skewed clonal X inactivation (median allelic cleavage ratio [ACR] of unmethylated/inactive M27 beta alleles was 50, range 1 to 100) or hypermethylation of both alleles. Two lymphomas showed random M27 beta X inactivation but clonal antigen-receptor gene rearrangements. In normal peripheral blood leukocytes from 105 healthy females aged 2 to 96 years, the median ACR was 2 (range 1 to 100). Thus, it was significantly lower than in the leukemias (P = .0001, Mann-Whitney test), but extremely skewed patterns (ACR > 10.8, ie, > 80th percentile) were seen not only in the leukemias but also in 21/105 samples (20%) of normal leukocytes, and significantly more frequent in a population of elderly women (aged 75 to 96 years) compared with healthy children (aged 2 to 8 years) and younger women (aged 20 to 58 years) (P = .00125; chi 2 test). We conclude that in a population of cells derived from the hematopoietic system where clonality is uncertain, skewed M27 beta patterns are not reliable indicators for the presence of a clonal neoplastic disorder. The basis for severe X-inactivation skewing is unclear at present, but this finding raises interesting questions regarding the composition of the hematopoietic stem cell pool.


Asunto(s)
Envejecimiento/fisiología , Hematopoyesis , Leucocitos/fisiología , Cromosoma X , Enfermedad Aguda , Adulto , Anciano , Anciano de 80 o más Años , Secuencia de Bases , Southern Blotting , Niño , Preescolar , Enfermedad Crónica , Clonación Molecular , Cartilla de ADN , Sondas de ADN , Femenino , Humanos , Leucemia/sangre , Leucemia/genética , Linfoma no Hodgkin/sangre , Linfoma no Hodgkin/genética , Metilación , Persona de Mediana Edad , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , Polimorfismo Genético , Valores de Referencia , Mapeo Restrictivo
2.
Am J Clin Pathol ; 98(3): 312-8, 1992 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-1326891

RESUMEN

A novel association, Epstein-Barr virus-positive Ki-1+/CD30+ anaplastic large cell non-Hodgkin's lymphoma of B-cell phenotype in immunosuppressed renal transplant recipients is reported. Case 1 involved an aggressive clinical evolution, whereas case 2 followed a more "benign" clinical course. Both lymphomas were Epstein-Barr virus-positive as assessed by in situ hybridization, Southern blot, polymerase chain reaction, and immunohistochemical analysis. Both lymphomas contained a single clonal Epstein-Barr virus terminal-repeat fragment. In case 1, clonality was confirmed by the detection of bi-allelic immunoglobulin (Ig) heavy chain gene rearrangement. Case 2 showed germline Ig genes at presentation and oligoclonal Ig heavy chain gene rearrangements at relapse. These results are consistent with the notion that anaplastic large cell lymphoma might arise in a B cell transformed by Epstein-Barr virus at a very early stage, before Ig gene rearrangement. The latter may occur later in the course of clonal evolution, thus permitting investigators to trace intermediate and late stages within a process of multistep lymphomagenesis and/or tumor progression.


Asunto(s)
Herpesvirus Humano 4/aislamiento & purificación , Trasplante de Riñón , Linfoma de Células B Grandes Difuso/microbiología , Adolescente , Adulto , Antígenos CD/análisis , Antígenos de Neoplasias/análisis , Southern Blotting , Sondas de ADN , ADN de Neoplasias/análisis , ADN Viral/análisis , Herpesvirus Humano 4/genética , Humanos , Inmunohistoquímica , Antígeno Ki-1 , Linfoma de Células B Grandes Difuso/genética , Linfoma de Células B Grandes Difuso/inmunología , Masculino , Hibridación de Ácido Nucleico , Reacción en Cadena de la Polimerasa , Complicaciones Posoperatorias/inmunología , Complicaciones Posoperatorias/microbiología
3.
Eur J Haematol ; 45(4): 215-22, 1990 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-1977612

RESUMEN

13 cases of childhood acute lymphoblastic leukaemia (ALL) were studied combining cell surface marker analysis with immunogenotyping by Southern blot hybridisation with a panel of antigen receptor gene probes. The immunophenotypes were unequivocal: 7 patients had B-phenotype and 6 patients T-phenotype ALL. In several patients immunogenotypes were not fully consistent with the respective phenotypes. For example, 2 B-cell precursor ALL had rearranged TCR beta chain genes and 2 T-ALL rearrangement of Ig heavy-chain genes. All cases showed clonal rearrangement or deletions within the TCR delta gene locus. TCR delta gene rearrangements might, therefore, serve as markers of clonality but not of B- or T-lineage in immature lymphoid neoplasms. We conclude that in current diagnostic practice immunogenotyping is a supplement rather than an alternative to immunophenotyping by surface marker analysis.


Asunto(s)
Proteínas Bacterianas , Linfoma de Burkitt/diagnóstico , Reordenamiento Génico , Genotipo , Leucemia-Linfoma de Células T del Adulto/diagnóstico , Receptores de Antígenos de Linfocitos T/genética , Adolescente , Linfoma de Burkitt/genética , Niño , Preescolar , Sondas de ADN , Desoxirribonucleasa EcoRI , Desoxirribonucleasa HindIII , Desoxirribonucleasas de Localización Especificada Tipo II , Reordenamiento Génico de Linfocito T , Reordenamiento Génico de la Cadena beta de los Receptores de Antígenos de los Linfocitos T , Humanos , Cadenas Pesadas de Inmunoglobulina/genética , Inmunofenotipificación , Lactante , Leucemia-Linfoma de Células T del Adulto/genética , Hibridación de Ácido Nucleico , Polimorfismo de Longitud del Fragmento de Restricción
6.
Biochem J ; 122(4): 405-8, 1971 May.
Artículo en Inglés | MEDLINE | ID: mdl-5001322

RESUMEN

An improved method for the isolation of normal immunoglobulin E is described. The method is based on the use of an immunoadsorbent formed by the mechanical entrapment of antibodies against a myeloma immunoglobulin E into a lattice of a highly cross-linked macroporous polyacrylamide gel. Normal immunoglobulin E was isolated from an immunoglobulin E-rich serum pool as well as from an individual healthy donor. The isolated immunoglobulin E was contaminated with about 20% of immunoglobulin G. An antiserum against normal immunoglobulin E was prepared by immunizing rabbits with the isolated immunoglobulin E.


Asunto(s)
Acrilatos , Adsorción , Inmunoglobulinas/aislamiento & purificación , Acrilamidas , Animales , Proteína de Bence Jones/aislamiento & purificación , Población Negra , Cromatografía por Intercambio Iónico , Dextranos , Electroforesis , Humanos , Sueros Inmunes , Inmunodifusión , Inmunoglobulina E/aislamiento & purificación , Inmunoglobulina G/aislamiento & purificación , Inmunoglobulina M/aislamiento & purificación , Mieloma Múltiple/inmunología , Conejos , Ovinos , Porcinos , Población Blanca
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA