Development of sequential and simultaneous bacterial cultures to hydrolyse and detoxify wood pre-hydrolysate for enhanced acetone-butanol-ethanol (ABE) production.

The use of microorganisms is a promising option for an eco-efficient and successful conversion of hardwood hemicelluloses to biofuels. The focus of this work is the treatment of hemicellulosic pre-hydrolysate by flocculation, followed by simultaneous or separate detoxification with Ureibacillus thermosphaericus and Cupriavidus taiwanensis co-culture, and hydrolysis with Paenibacillus campinasensis. A reduction of phenolic compounds was achieved mainly after flocculation, applied as a first detoxification step, but no increase in sugars concentration was observed. The ABE fermentation of the hydrolysate obtained from the simultaneous hydrolysis and detoxification produced 6.8 g L-1 of butanol after 116 h, which was higher than that generated with xylose synthetic medium. The higher biofuel concentration in the hydrolysate is attributed to the existence of carbon sources, other than xylose.

Combining chemical flocculation and bacterial co-culture of Cupriavidus taiwanensis and Ureibacillus thermosphaericus to detoxify a hardwood hemicelluloses hydrolysate and enable acetone-butanol-ethanol fermentation leading to butanol.

Butanol, a fuel with better characteristics than ethanol, can be produced via acetone-butanol-ethanol (ABE) fermentation using lignocellulosic biomass as a carbon source. However, many inhibitors present in the hydrolysate limit the yield of the fermentation process. In this work, a detoxification technology combining flocculation and biodetoxification within a bacterial co-culture composed of Ureibacillus thermosphaericus and Cupriavidus taiwanensis is presented for the first time. Co-culture-based strategies to detoxify filtered and unfiltered hydrolysates have been investigated. The best results of detoxification were obtained for a two-step approach combining flocculation to biodetoxification. This sequential process led to a final phenolic compounds concentration of 1.4 g/L, a value close to the minimum inhibitory level observed for flocculated hydrolysate (1.1 g/L). The generated hydrolysate was then fermented with Clostridium acetobutylicum ATCC 824 for 120 h. A final butanol production of 8 g/L was obtained, although the detoxified hydrolysate was diluted to reach 0.3 g/L of phenolics to ensure noninhibitory conditions. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 35: e2753, 2019.