RESUMEN
We previously demonstrated that maintenance of cell elongation in the apical region of maize primary roots at low water potentials (psi(w)) was associated with an increase in expansin activity and extractable expansin protein. Here, we characterized the spatial pattern of expansin gene expression along the growing maize root and studied the effect of low psi(w) on expansin gene expression. Roots were divided into three segments: apical 0 to 5 mm, subapical 5 to 10 mm, and non-growing 10 to 20 mm. Of the five expansin genes expressed in control roots, two alpha-expansins (Exp1 and Exp5) and two beta-expansins (ExpB2 and ExpB8) are expressed specifically in the growing region, whereas expression of beta-expansin ExpB6 is shifted basipetally. After seedlings were transplanted to vermiculite with a psi(w) of -1.6 MPa, transcripts for Exp1, Exp5, and ExpB8 rapidly accumulated in the apical region of the root. These mRNA changes correlated with the maintenance of root elongation and increases in wall extensibility found previously. The beta-expansins ExpB2 and ExpB6 showed distinctive patterns of expression and responses to low psi(w,) indicative of distinctive functions. Inhibition of abscisic acid (ABA) accumulation at low psi(w) (by fluridone treatment) had no effect on expansin expression, except that ExpB2 transcript level showed a minor dependence on ABA. Gene-specific regulation of alpha- and beta-expansin mRNA pools likely contributes to growth alterations of the maize (Zea mays) root as it adapts to a low psi(w), but these changes do not appear to be mediated by changes in ABA content.
Asunto(s)
Regulación de la Expresión Génica de las Plantas , Proteínas de Plantas/genética , Raíces de Plantas/genética , Zea mays/genética , Ácido Abscísico/metabolismo , Adaptación Fisiológica , Northern Blotting , Perfilación de la Expresión Génica , Genes de Plantas , Familia de Multigenes , Presión Osmótica , Reguladores del Crecimiento de las Plantas/metabolismo , Proteínas de Plantas/metabolismo , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/metabolismo , ARN de Planta/análisis , Agua/metabolismo , Zea mays/efectos de los fármacos , Zea mays/metabolismoRESUMEN
Brucella abortus strain RB51 is an approved brucellosis vaccine for use in cattle that may have potential as an oral vaccine for use in elk (Cervus elaphus) and/or bison (Bison bison). This study was designed to determine effects of strain RB51 on deer mice (Peromyscus maniculatus), a nontarget species that could have access to treated baits in a field situation. In February 1994, 90 mice were orally dosed or intraperitoneally injected with 1 x 10(8) colony forming units strain RB51 and 77 controls were similarly dosed with sterile saline. At weekly intervals through early April 1994, 4 to 6 mice from each group were euthanized, gross necropsies performed, spleens and uteruses cultured, and tissues examined histologically. All orally inoculated mice cleared the infection by 6 wk post-inoculation (PI). While most of the injected mice cleared the infection by 7 wk PI, a few required 9 wk. There were minimal adverse effects attributable to strain RB51. Apparently, strain RB51 would not negatively impact P. maniculatus populations if it were used in a field situation. Also, deer mice appear to be able to clear the vaccine in 6 to 9 wk, thus the probability of these mice transmitting the vaccine to other animals is low.
Asunto(s)
Vacuna contra la Brucelosis/normas , Brucella abortus/inmunología , Brucelosis/veterinaria , Peromyscus/inmunología , Administración Oral , Animales , Vacuna contra la Brucelosis/administración & dosificación , Vacuna contra la Brucelosis/farmacocinética , Brucelosis/patología , Brucelosis/prevención & control , Recuento de Colonia Microbiana , Femenino , Inyecciones Intraperitoneales/veterinaria , Masculino , Distribución Aleatoria , Seguridad , Bazo/microbiología , Bazo/patología , Útero/microbiología , Útero/patologíaRESUMEN
Surveillance and epidemic modeling were used to study chronic wasting disease (CWD), a transmissible spongiform encephalopathy that occurs naturally among sympatric, free-ranging deer (Odocoileus spp.) and Rocky Mountain elk (Cervus elaphus nelsoni) populations in contiguous portions of northeastern Colorado and southeastern Wyoming (USA). We used clinical case submissions to identify endemic areas, then used immunohistochemistry to detect CWD-infected individuals among 5,513 deer and elk sampled via geographically-focused random surveys. Estimated overall prevalence (prevalence, 95% confidence interval) in mule deer (4.9%, 4.1 to 5.7%) was higher than in white-tailed deer (2.1%, 0.5 to 3.4%) or elk (0.5%, 0.001 to 1%) in endemic areas; CWD was not detected in outlying portions of either state. Within species, CWD prevalence varied widely among biologically- or geographically-segregated subpopulations within the 38,137 km2 endemic area but appeared stable over a 3-yr period. The number of clinical CWD cases submitted from an area was a poor predictor of local CWD prevalence, and prevalence was typically > or =1% before clinical cases were first detected in most areas. Under plausible transmission assumptions that mimicked field data, prevalence in epidemic models reached about 1% in 15 to 20 yr and about 15% in 37 to 50 yr. Models forecast population declines once prevalence exceeded about 5%. Both field and model data supported the importance of lateral transmission in CWD dynamics. Based on prevalence, spatial distribution, and modeling, we suggest CWD has been occurring in northeastern Colorado and southeastern Wyoming for >30 yr, and may be best represented as an epizootic with a protracted time-scale.
Asunto(s)
Ciervos , Modelos Biológicos , Síndrome Debilitante/veterinaria , Animales , Animales Salvajes , Anticuerpos Monoclonales , Colorado/epidemiología , Femenino , Inmunohistoquímica , Masculino , Bulbo Raquídeo/patología , Dinámica Poblacional , Prevalencia , Síndrome Debilitante/epidemiología , Wyoming/epidemiologíaRESUMEN
To examine whether the reduced shoot growth of abscisic acid (ABA)-deficient mutants of tomato is independent of effects on plant water balance, flacca and notabilis were grown under controlled-humidity conditions so that their leaf water potentials were equal to or higher than those of well-watered wild-type plants throughout development. Most parameters of shoot growth remained markedly impaired and root growth was also greatly reduced. Additional experiments with flacca showed that shoot growth substantially recovered when wild-type levels of ABA were restored by treatment with exogenous ABA, even though improvement in leaf water potential was prevented. The ability of applied ABA to increase growth was greatest for leaf expansion, which was restored by 75%. The ethylene evolution rate of growing leaves was doubled in flacca compared to the wild type and treatment with silver thiosulphate to inhibit ethylene action partially restored shoot growth. The results demonstrate that normal levels of endogenous ABA are required to maintain shoot development, particularly leaf expansion, in well-watered tomato plants, independently of effects on plant water balance. The impairment of shoot growth caused by ABA deficiency is at least partly attributable to ethylene.
Asunto(s)
Ácido Abscísico/fisiología , Etilenos/metabolismo , Brotes de la Planta/crecimiento & desarrollo , Solanum lycopersicum/crecimiento & desarrollo , AguaRESUMEN
Some of the elk (Cervus elaphus nelsoni) of the Greater Yellowstone Area (Wyoming, Idaho, Montana; USA) are infected with Brucella abortus, the bacterium that causes bovine brucellosis. Brucella abortus strain RB51 vaccine is being considered as a means to control B. abortus induced abortions in cow elk. However, the most probable vaccination strategies for use in free-ranging elk might also result in some bull elk being inoculated, thus, it is important to insure that the vaccine is safe in these animals. In the winter of 1995, 10 free-ranging bull elk calves were captured, tested for B. abortus antibodies, and intramuscularly inoculated with 1.0 x 10(9) colony forming units (CFU) of B. abortus strain RB51. Blood was collected for hemoculture and serology every 2 wk after inoculation for 14 wk. Beginning 4 mo postinoculation and continuing until 10 mo postinoculation elk were serially euthanized, necropsied, and tissues collected for culture and histopathology. These elk cleared the organism from the blood within 6 wk and from all tissues within 10 mo. No lesions attributable to B. abortus were found grossly and only minimal to mild lymphoplasmacytic epididymitis was found in a few elk on histologic examination. In a separate study, six adult bull elk from Wind Cave National Park (South Dakota, USA) were taken to a ranch near Carrington (North Dakota, USA). Three were orally inoculated with approximately 1.0 x 10(10) CFU of RB51 and three were inoculated with corn syrup and saline. Ninety days post-inoculation semen was examined and cultured from these bulls. Strain RB51 was not cultured from their semen at that time. There were no palpable abnormalities in the genital tract and all elk produced viable sperm. Although they contain small sample sizes, these studies suggest that B. abortus strain RB51 is safe in bull elk.
Asunto(s)
Vacuna contra la Brucelosis , Brucella abortus/inmunología , Brucelosis/veterinaria , Ciervos , Animales , Anticuerpos Antibacterianos/sangre , Vacuna contra la Brucelosis/normas , Brucella abortus/aislamiento & purificación , Brucelosis/prevención & control , Ensayo de Inmunoadsorción Enzimática/veterinaria , Masculino , SeguridadRESUMEN
Wyoming toads (Bufo baxteri) that died from January 1989 to June 1996 were submitted to the Wyoming State Veterinary Laboratory (Laramie, Wyoming, USA) for postmortem evaluation. These consisted of 108 free-ranging toads and 170 animals from six captive populations. Ninety-seven (90%) of 108 free-ranging toad carcasses were submitted during September and October. From 1989 to 1992, 27 (77%) of 35 mortalities in the captive populations occurred in October, November, and December. From 1993 to 1996, mortality in captive toads occurred without a seasonal pattern and coincided with changes in hibernation protocols that no longer mimicked natural cycles. Cause of mortality was determined in 147 (53%) of the 278 cases. Mycotic dermatitis with secondary bacterial septicemia was the most frequent diagnosis in 104 (71%) of 147 toads. Basidiobolus ranarum was found by microscopic examination of skin sections in 100 (96%) of 104 of these mortalities. This fungus was isolated from 30 (56%) of 54 free-ranging and 24 (48%) of 50 captive toads. This research documents the causes of mortality for both free-ranging and captive endangered Wyoming toads over a 7 yr period.
Asunto(s)
Bufonidae , Dermatomicosis/veterinaria , Entomophthorales/aislamiento & purificación , Sepsis/veterinaria , Cigomicosis/veterinaria , Factores de Edad , Animales , Causas de Muerte , Dermatomicosis/complicaciones , Dermatomicosis/mortalidad , Femenino , Masculino , Sepsis/etiología , Sepsis/mortalidad , Factores Sexuales , Wyoming/epidemiología , Cigomicosis/complicaciones , Cigomicosis/mortalidadRESUMEN
Captive carnivores are susceptible to a wide array of infectious and parasitic diseases, which reflects the diversity of the seven families of Carnivora. Unfortunately, relatively few in-depth studies have been conducted on diseases of non-domestic carnivores, and much remains to be learned, especially regarding diseases of small carnivores (e.g. mustelids, viverrids and procyonids). The more important infectious diseases of carnivores include rabies, canine distemper, and diseases caused by parvoviruses, coronaviruses and herpesviruses. Few parasitic or bacterial pathogens are significant in captive populations, and appropriate husbandry, therapy, vaccines and quarantine minimize the risk of disease. Extrapolations from one species to another regarding disease susceptibility may be incorrect. The black-footed ferret (Mustela nigripes) serves as an example of a carnivore significantly affected by infectious diseases, some of which were expected while others could not have been predicted from generalized knowledge of diseases of mustelids. This highlights the need to understand the natural history of each species maintained in captivity.
Asunto(s)
Animales de Zoológico , Infecciones Bacterianas/veterinaria , Carnívoros , Hurones , Enfermedades Parasitarias en Animales , Virosis/veterinaria , Animales , Infecciones Bacterianas/epidemiología , Humanos , Enfermedades Parasitarias/epidemiología , Virosis/epidemiología , ZoonosisRESUMEN
Diagnosis of sylvatic plague in a captive black-footed ferret (Mustela nigripes) was based on gross and microscopic lesions, fluorescent antibody tests, culture of Yersinia pestis, and immunohistochemistry. Gross lesions consisted of acute hemorrhage and necrosis associated with cervical and mesenteric lymph nodes, subcutaneous hemorrhages, and pulmonary edema. Acute edema, hemorrhage, and necrosis with numerous bacteria in blood vessels and sinusoids characterized microscopic lesions. Occurrence of fatal plague in a black-footed ferret potentially has significant implications for recovery of this endangered species due to the widespread distribution of plague in prairie dog colonies throughout historic black-footed ferret range.
Asunto(s)
Hurones , Peste/veterinaria , Animales , Pulmón/microbiología , Pulmón/patología , Masculino , Peste/epidemiología , Peste/patología , Wyoming/epidemiología , Yersinia pestis/aislamiento & purificaciónRESUMEN
Brucellosis was studied opportunistically in bison (Bison bison) in the free-ranging Jackson herd of approximately 120 in Teton County, Wyoming (USA) in March 1989. Recent abortion was diagnosed in a 2-yr-old cow and Brucella abortus biovar 1 was isolated from vaginal discharge, uterine contents, uterus, and supramammary lymph nodes. Endometritis was characterized by lymphoplasmacytic infiltrates in the lamina propria and neutrophils in uterine glands and within necrotic debris and exudate in the uterine lumen. A 5-yr-old bull had diffuse lymphoplasmacytic infiltrates in epididymis and accessory sex glands; B. abortus was isolated from seminal vesicle and ampulla. Twenty-seven (77%) of 35 bison tested from 1989 to 1990 were serologically positive or suspect on tests for Brucella antibodies. We report the occurrence of abortion due to brucellosis in free-ranging bison in the Jackson herd, suggest that bison in this herd are capable of transmitting brucellosis to other susceptible hosts, and report the first confirmation of brucellosis in this herd.
Asunto(s)
Aborto Veterinario/microbiología , Bison , Brucella abortus/aislamiento & purificación , Brucelosis/veterinaria , Aborto Veterinario/patología , Animales , Animales Salvajes , Brucelosis/epidemiología , Brucelosis/microbiología , Brucelosis/patología , Femenino , Masculino , Embarazo , Prevalencia , Wyoming/epidemiologíaRESUMEN
Vaginal cytology and vulva size were used to characterize the reproductive cycle of female black-footed ferrets (Mustela nigripes), Siberian polecats (M. eversmanni), and domestic ferrets (M. putorius furo). Emphasis was on black-footed ferrets because of the need to breed these critically endangered animals and on Siberian polecats because of the close taxonomic relationship to black-footed ferrets. Vaginal cytology of the 3 species of ferret is similar. Proestrus was characterized by an increasing percentage of superficial epithelial cells and enlargement of the vulva. During estrus, superficial cells were usually greater than or equal to 90% of epithelial cells in the vaginal lavage and after several days were fully keratinized. Neutrophils were more common during all stages of the estrous cycle in domestic ferrets than they were in the other species. Following copulation, percentage of superficial calls in the vagina declined and vulva swelling subsided. Large cells, probably of uterine symplasma origin, were observed in vaginal lavages following whelping or pseudopregnancy. Vaginal cytology is extremely useful in the reproductive management of black-footed ferrets and Siberian polecats. Knowledge of normal vaginal cytology could be applied to the diagnosis of female reproductive abnormalities in all 3 species.
Asunto(s)
Estro , Hurones/fisiología , Vagina/citología , Animales , Recuento de Células/veterinaria , Células Epiteliales , Femenino , Neutrófilos/citología , Valores de Referencia , Vulva/anatomía & histologíaRESUMEN
Sperm were obtained via electroejaculation from Domestic ferret, (Mustela putorius furo), Siberian ferret (M. eversmanni), Black-footed ferret (M. nigripes), and a hybrid between Siberian and Domestic, called the Fitch ferret (M. sp.). Comparisons of sperm were made by four different microscopy techniques to determine whether differences exist among species. First, Nomarski differential interference microscopy could be used to distinguish domestic ferret sperm from the others on the basis of the structure of the posterior part of the acrosome. Second, both silver staining, which demonstrates argentophilic protein distribution, and scanning electron microscopy (SEM), revealed differences among the morphology of sperm for each species; variation in the unique appearance of the acrosome in ferret sperm was detected especially well by SEM. To quantify differences in morphology, five sperm head parameters were measured using image analysis; light microscopy produced significantly larger values than did SEM (all parameters and all species but Fitch), and there were significant differences owing to species for all parameters but one. Generally, our data demonstrate the value of complementary techniques to distinguish among sperm of closely related species and more specifically may help establish evolutionary relationships among the ferret species studied. In addition, they provide baseline data important for the captive breeding of the endangered Black-footed ferret.
Asunto(s)
Acrosoma/ultraestructura , Hurones/anatomía & histología , Espermatozoides/ultraestructura , Animales , Evolución Biológica , Hurones/clasificación , Procesamiento de Imagen Asistido por Computador , Masculino , Microscopía Electrónica de Rastreo , Microscopía de Interferencia , Tinción con Nitrato de PlataRESUMEN
Eight domestic ferrets (Mustela putorius furo) and two Siberian polecats (M. eversmanni) were inoculated subcutaneously with 12 to 1.2 x 10(7) Yersinia pestis originally isolated during an epizootic of plague in white-tailed prairie dogs (Cynomys leucurus) near Meeteetse, Park County, Wyoming (USA) in 1985. None of the ferrets or polecats developed clinical signs of disease which suggested that black-footed ferrets (M. nigripes), a congener, also would be resistant to plague. All animals receiving greater than or equal to 1.2 X 10(3) organisms produced serum antibodies detected by the passive hemagglutination test with titers peaking at 1:1,024 and remaining positive until at least 219 days postinoculation. Sera collected from 12 free-ranging black-footed ferrets near Meeteetse in 1984 and 1985 were negative for antibodies against Y. pestis. Prevalence of antibodies against Y. pestis was high in other carnivores collected from the same area in 1986.
Asunto(s)
Carnívoros , Hurones , Peste/veterinaria , Animales , Animales Domésticos , Animales Salvajes , Anticuerpos Antibacterianos/sangre , Susceptibilidad a Enfermedades/veterinaria , Femenino , Inmunidad Innata , Dosificación Letal Mediana , Masculino , Mephitidae , Peste/epidemiología , Peste/inmunología , Prevalencia , Mapaches , Virulencia , Yersinia pestis/inmunología , Yersinia pestis/aislamiento & purificación , Yersinia pestis/patogenicidadRESUMEN
Thirteen Rocky Mountain bighorn sheep (Ovis canadensis canadensis) with clinical signs of psoroptic scabies were captured in Wyoming. Signs included droopy ears, depilation on the head and neck, and exudate in the ears. Mites were identified as either Psoroptes cervinus or P. equi. Two ewes with scabies at the time of original capture had no clinical signs of mite infection 1 and 2 yr later.
Asunto(s)
Infestaciones por Ácaros/veterinaria , Rumiantes/parasitología , Animales , Oído Externo/parasitología , Femenino , Cabeza/parasitología , Masculino , Infestaciones por Ácaros/epidemiología , Piel/parasitología , Wyoming/epidemiologíaRESUMEN
Trophozoites and cysts of an amoeba resembling Entamoeba bovis were recovered from soft stools of captive pronghorn fawns (Antilocapra americana). Chronic or intermittent diarrhea was observed in most individuals in two groups of hand-raised 1- to 8-mo-old pronghorns. Ileocecal lymph nodes were mildly to moderately enlarged. Microscopic lesions were characterized by lymphoid hyperplasia, focal necrosis and pyogranulomatous inflammation in lymph nodes and focal necrosis and diffuse nonsuppurative enteritis in ileum and cecum. In 12 of 17 fawns, trophic stages of amoebae were observed in the submucosa of the cecum and/or the cortex of the ileocecal lymph node. This is the first report of E. bovis-like organisms invading and causing pathological changes in the tissues of their host.
Asunto(s)
Amebiasis/veterinaria , Artiodáctilos/parasitología , Entamebiasis/veterinaria , Parasitosis Intestinales/veterinaria , Animales , Ciego/parasitología , Ciego/patología , Diarrea/parasitología , Diarrea/veterinaria , Entamoeba/aislamiento & purificación , Entamebiasis/epidemiología , Entamebiasis/patología , Heces/parasitología , Parasitosis Intestinales/epidemiología , Parasitosis Intestinales/patología , Intestino Delgado/parasitología , Intestino Delgado/patología , Ganglios Linfáticos/parasitología , Ganglios Linfáticos/patología , Wyoming/epidemiologíaRESUMEN
A large osteochondroma of the skull was diagnosed in a young adult free-ranging white-tailed deer (Odocoileus virginianus) from Wyoming. The 4.8-kg neoplasm primarily involved the left frontal bone with extension into the cranial cavity. Microscopically, the neoplasm was composed of well differentiated trabecular bone and islands of cartilage. This neoplasm is rare in domestic species and has not been reported in cervids. Though benign in morphology, the location and size of the neoplasm was probably responsible for debilitation of the deer.
Asunto(s)
Condroma/veterinaria , Ciervos , Neoplasias Craneales/veterinaria , Animales , Condroma/patología , Femenino , Neoplasias Craneales/patologíaRESUMEN
In September and October 1985, six black-footed ferrets (Mustela nigripes) were captured from the only known population, located near Meeteetse, Wyoming for captive propagation. Two days following capture an adult male showed signs of canine distemper and an adult female displayed similar signs 7 days postcapture; these infections were undoubtedly acquired prior to capture. Subsequently the four remaining captive black-footed ferrets also developed canine distemper and all eventually died. Clinical signs included severe pruritus, hyperkeratosis and progressive loss of body condition. A few animals had intermittent diarrhea and respiratory disease. Intranuclear and intracytoplasmic inclusion bodies were numerous in epithelial tissues and two black-footed ferrets had a mild to moderate meningoencephalitis. Canine distemper virus was isolated from four animals and paramyxovirus nucleocapsids were observed by electron microscopy of feces from all affected black-footed ferrets. Antibodies to canine distemper virus were not detected in sera of sick black-footed ferrets. Antibodies to canine distemper virus were found in sera of badgers (Taxidea taxus) and coyotes (Canis latrans) collected in the Meeteetse area in 1986. Most free-ranging black-footed ferrets in the colony apparently died of canine distemper during the summer and fall of 1985. An attempt was made to capture all surviving animals in the affected area in order to abort the epizootic and provide black-footed ferrets for captive propagation.
Asunto(s)
Carnívoros/microbiología , Moquillo/patología , Hurones/microbiología , Animales , Animales Domésticos/microbiología , Animales Salvajes/microbiología , Coccidiosis/etiología , Coccidiosis/veterinaria , Brotes de Enfermedades/veterinaria , Moquillo/complicaciones , Moquillo/epidemiología , Moquillo/inmunología , Moquillo/mortalidad , Virus del Moquillo Canino/inmunología , Virus del Moquillo Canino/aislamiento & purificación , Femenino , Giardiasis/etiología , Giardiasis/veterinaria , Masculino , Peste/veterinaria , Vigilancia de la Población/veterinaria , Prurito/microbiología , Prurito/veterinaria , Roedores/microbiología , Factores de Tiempo , WyomingRESUMEN
At least 3,200 pronghorn (Antilocapra americana) died during a bluetongue (BT) epizootic in eastern Wyoming during late September and early October 1976. In August and September 1984, another BT epizootic occurred in northeastern Wyoming resulting in 300 known pronghorn deaths. In 17 pronghorn examined postmortem, hemorrhages and edema were the most common gross pathologic changes. Microscopic changes included hemorrhage, edema, arterial fibrinoid necrosis, lymphoid depletion in splenic and lymphatic follicles, and neuronal necrosis. Bluetongue virus serotype 17 was isolated from pronghorn in both epizootics. Mortalities ceased with the advent of cool weather in late September and October. Seventy-six of 94 pronghorn killed by hunters during the latter period of the 1976 epizootic, and 14 of 24 pronghorn killed 1 yr later had serologic evidence of exposure to BT virus. The reproductive rate in pronghorn was depressed to 47 fawns per 100 does in August 1977, but returned to 93 fawns per 100 does by 1978. Following the 1984 outbreak, the reproductive rate was similarly depressed, but the cause was confounded by other environmental and range conditions. Deer, mostly mule deer (Odocoileus hemionus), also died during both epizootics of what was presumed to be BT.
Asunto(s)
Artiodáctilos , Lengua Azul/epidemiología , Animales , Anticuerpos Antivirales/análisis , Lengua Azul/mortalidad , Lengua Azul/patología , Virus de la Lengua Azul/inmunología , Virus de la Lengua Azul/aislamiento & purificación , Ciervos , Femenino , Fertilidad , Masculino , WyomingRESUMEN
A mixture of 120 mg ketamine hydrochloride (KHCL)/20 mg xylazine hydrochloride (XHCL)/ml was used to immobilize 37 wild mountain lions (Felis concolor) 46 times. Observations were recorded during 37 trials that included kittens, adult females, and adult males. Dosages were based on 11 mg KHCL and 1.8 mg XHCL/kg estimated body weight. Actual doses for 24 lions requiring a single injection for immobilization ranged from 4.7-15.8 mg KHCL/kg and 0.8-2.6 mg XHCL/kg. Induction, duration, and recovery times did not differ (P greater than 0.05) between the sex and age classes. Two kittens were overdosed with the drug combination, but the effects were not life threatening. Eleven other lions, nine of which were initially underdosed, required additional injections of the drug combination for safe handling. Immobilization was characterized initially by semi-consciousness, open eyelids, pupillary dilation, and muscle rigidity. Later, most lions appeared unconscious, muscles relaxed, and breathing slowed considerably. No convulsions or hypersalivation occurred. The KHCL/XHCL mixture given at approximately 11 mg KHCL and 1.8 mg XHCL/kg body weight proved useful for immobilizing wild mountain lions for research purposes. Suggestions for case of immobilized cats are included.