RESUMEN
The Human Silencing Hub (HUSH) complex is necessary for epigenetic repression of LINE-1 elements. We show that HUSH-depletion in human cell lines and primary fibroblasts leads to induction of interferon-stimulated genes (ISGs) through JAK/STAT signaling. This effect is mainly attributed to MDA5 and RIG-I sensing of double-stranded RNAs (dsRNAs). This coincides with upregulation of primate-conserved LINE-1s, as well as increased expression of full-length hominid-specific LINE-1s that produce bidirectional RNAs, which may form dsRNA. Notably, LTRs nearby ISGs are derepressed likely rendering these genes more responsive to interferon. LINE-1 shRNAs can abrogate the HUSH-dependent response, while overexpression of an engineered LINE-1 construct activates interferon signaling. Finally, we show that the HUSH component, MPP8 is frequently downregulated in diverse cancers and that its depletion leads to DNA damage. These results suggest that LINE-1s may drive physiological or autoinflammatory responses through dsRNA sensing and gene-regulatory roles and are controlled by the HUSH complex.
Asunto(s)
Epigénesis Genética/fisiología , Regulación Neoplásica de la Expresión Génica , Silenciador del Gen/fisiología , Interferón Tipo I/metabolismo , Elementos de Nucleótido Esparcido Largo/fisiología , Proteína 58 DEAD Box/genética , Proteína 58 DEAD Box/metabolismo , Daño del ADN , Regulación hacia Abajo , Técnicas de Inactivación de Genes , Células HEK293 , Células HeLa , Humanos , Inflamación , Helicasa Inducida por Interferón IFIH1/metabolismo , Elementos de Nucleótido Esparcido Largo/genética , Fosfoproteínas/metabolismo , ARN Bicatenario , Receptores Inmunológicos , Análisis de Secuencia de ARN , Transducción de SeñalRESUMEN
Retrotransposons encompass half of the human genome and contribute to the formation of heterochromatin, which provides nuclear structure and regulates gene expression. Here, we asked if the human silencing hub (HUSH) complex is necessary to silence retrotransposons and whether it collaborates with TRIM28 and the chromatin remodeler ATRX at specific genomic loci. We show that the HUSH complex contributes to de novo repression and DNA methylation of an SVA retrotransposon reporter. By using naïve versus primed mouse pluripotent stem cells, we reveal a critical role for the HUSH complex in naïve cells, implicating it in programming epigenetic marks in development. Although the HUSH component FAM208A binds to endogenous retroviruses (ERVs) and long interspersed element-1s (LINE-1s or L1s), it is mainly required to repress evolutionarily young L1s (mouse-specific lineages <5 million years old). TRIM28, in contrast, is necessary to repress both ERVs and young L1s. Genes co-repressed by TRIM28 and FAM208A are evolutionarily young, or exhibit tissue-specific expression, are enriched in young L1s, and display evidence for regulation through LTR promoters. Finally, we demonstrate that the HUSH complex is also required to repress L1 elements in human cells. Overall, these data indicate that the HUSH complex and TRIM28 co-repress young retrotransposons and new genes rewired by retrotransposon noncoding DNA.
Asunto(s)
Genoma Humano , Proteínas Nucleares/genética , Retroelementos/genética , Proteína 28 que Contiene Motivos Tripartito/genética , Animales , Metilación de ADN/genética , Retrovirus Endógenos/genética , Heterocromatina/genética , Humanos , Elementos de Nucleótido Esparcido Largo/genética , Ratones , Regiones Promotoras GenéticasRESUMEN
Cancer cells thrive on genetic and epigenetic changes that confer a selective advantage but also need strategies to avoid immune recognition. In this issue, Cuellar et al. (2017. J. Cell Biol https://doi.org/10.1083/jcb.201612160) find that the histone methyltransferase SETDB1 enables acute myeloid leukemia cells to evade sensing of retrotransposons by innate immune receptors.