RESUMEN
In 82 clinical strains of Streptococcus pyogenes (group A streptococci) isolated from patients with various manifestations of streptococcal infection, emm-typing revealed 27 emm-types (n=77) with a predominance of emm-89 (n=15; 18%), emm-75 (n=9; 11%), and emm-1 (n=6; 7%); types emm-3, emm-12, and emm-58 (n=4; 5% each) were found with almost equal frequency; other types were less common. The superantigen genes speC, speG, speH, speI, speJ, speK, speL, speM, smeZ, and SSA were identified in S. pyogenes strains using multiprimer PCR; the genes of the superantigen SpeA and cysteine proteinase SpeB were detected using real-time PCR. All the studied S. pyogenes strains contained superantigen genes, and 98% of the strains had several (from 2 to 7) genes. The number of variants of these sets reached 37; 2% of the strains contained only one superantigen gene. The distribution frequencies of superantigen genes in the studied strains were: speA - 43%; speC - 38%; speG - 93%; speH - 13%; speI - 6%; speJ - 24%; speK - 13%; speL and speM - 11% each; smeZ - 98%; SSA - 15%. All studied S. pyogenes strains contained the speB gene. Our studies have demonstrated that the sets of superantigen genes of group A streptococci are characterized by pronounced diversity to some extent associated with emm-type.
Asunto(s)
Infecciones Estreptocócicas , Streptococcus pyogenes , Humanos , Streptococcus pyogenes/genética , Antígenos Bacterianos/genética , Reacción en Cadena en Tiempo Real de la Polimerasa , Superantígenos/genética , Biología Molecular , Proteínas de la Membrana Bacteriana Externa/genéticaRESUMEN
A process flow diagram was elaborated for production and control of tissue culture inactivated vaccine (TCIV) against Japanese encephalitis (JE). The vaccine was prepared on the basis of the earlier patented JE virus strain (K3). Experimental laboratory JE TCIV series were obtained; their safety and high immunogenicity were tested on animals. Regulations (an instruction) for preparing and controlling JE TCIV have been worked out, which have been approved by the Academic Council of the D. I. Ivanovsky Institute of Virology.
Asunto(s)
Virus de la Encefalitis Japonesa (Especie)/inmunología , Encefalitis Japonesa/inmunología , Encefalitis Japonesa/prevención & control , Inmunización , Vacunas contra la Encefalitis Japonesa/inmunología , Vacunas contra la Encefalitis Japonesa/aislamiento & purificación , Vacunas contra la Encefalitis Japonesa/toxicidad , Animales , Anticuerpos Antivirales/sangre , Anticuerpos Antivirales/inmunología , Células Cultivadas , Embrión de Pollo , Encefalitis Japonesa/sangre , Formaldehído , Cobayas , Pruebas de Inhibición de Hemaglutinación , Esquemas de Inmunización , Inyecciones Intramusculares , Inyecciones Intraperitoneales , Vacunas contra la Encefalitis Japonesa/administración & dosificación , Ratones , Pruebas de Neutralización , ConejosRESUMEN
The patterns of RNA from cells infected with avian adenovirus chicken embryo lethal orphan (CELO) virus were analyzed by Northern hybridization method. Early RNAs are specifically hybridized with several fragments of the Eco-RI restriction map: A (49-81%), B (0.2-20.3%), D (81-92.5%), and E fragments of the Bam-HI CELO DNA restriction map. Early RNAs were not hybridized with C (37.8-49%), G (31-37.8%), and E (20.3-31%) fragments of the Eco-RI DNA CELO restriction map. At least 19 types of virus-specific RNA molecules homologous to 4 different regions of the virus genome are produced in CELO-infected permissive cells. Several classes of virus-specific RNAs were identified in CELO virus-transformed cell strains.
Asunto(s)
Aviadenovirus/genética , Genoma Viral , Transcripción Genética , Animales , Northern Blotting , Células Cultivadas , Embrión de Pollo , ARN Mensajero/genética , Mapeo RestrictivoRESUMEN
A 3.5-kilobase DNA fragment of the fowl adenovirus type 1 (CELO), located between map units 31.1 and 39.4 has been determined. The sequence contains the probable CELO equivalents of the IIIa protein, penton base, pVII and pV core protein genes of human adenovirus (HAV). The CELO penton base and major core protein (analog HAV pVII) were found to consist of 514 (56.8 kDa) and 72 amino acids (8.4 kDa), respectively.
Asunto(s)
Adenoviridae/genética , Aviadenovirus/genética , Proteínas de la Cápside , Cápside/genética , Genes Virales , Proteínas del Núcleo Viral/genética , Secuencia de Aminoácidos , Animales , Aviadenovirus/metabolismo , Secuencia de Bases , Cápside/biosíntesis , Células Cultivadas , Pollos , Secuencia Conservada , Cartilla de ADN , ADN Viral , Genoma Viral , Humanos , Riñón , Datos de Secuencia Molecular , Peso Molecular , Reacción en Cadena de la Polimerasa , ARN Mensajero/análisis , ARN Mensajero/biosíntesis , Mapeo Restrictivo , Homología de Secuencia de Aminoácido , Proteínas del Núcleo Viral/biosíntesisRESUMEN
Reciprocal competition of viral and cellular matrices of RNA synthesis was demonstrated, as well as a 10-13-fold increase of 3H-uridine incorporation into viral RNAs in the presence of actinomycin D, the inhibiting effect of actinomycin D on virus replication, the dependence of the late stage of Japanese encephalitis virus reproduction upon the host cell, and no positive effect of actinomycin D on generation of Japanese encephalitis virus ts mutants.