RESUMEN
Foot-and-mouth disease (FMD) is endemic in Kenya where four serotypes (O, A, SAT 1 and SAT 2) of the virus are currently in circulation. Within 2010 and 2011, the National Laboratory recorded an increase in the number of FMD outbreaks caused by serotype O virus. The characteristics of these viruses were determined to ascertain whether these were independent outbreaks or one single strain spreading throughout the country. The sequences of the complete VP1-coding region were analysed from viruses sampled within different areas of Kenya during 2010 and 2011. The results indicated that the 2010 to 2011 outbreaks in Kenya were caused by four independent strains. By comparison with earlier type O isolates from Eastern Africa, it was apparent that the outbreaks were caused by viruses from three different lineages of topotype EA-2 and a fourth virus strain belonging to topotype EA-4. The topotypes EA-1 and EA-3 were not detected from these outbreaks. Implications of these results for FMD control in Eastern Africa are discussed.
Asunto(s)
Virus de la Fiebre Aftosa/genética , Fiebre Aftosa/virología , Animales , Bovinos , Brotes de Enfermedades/veterinaria , Fiebre Aftosa/epidemiología , Virus de la Fiebre Aftosa/clasificación , Virus de la Fiebre Aftosa/aislamiento & purificación , Variación Genética , Kenia/epidemiología , Filogenia , ARN Viral/genética , Reacción en Cadena en Tiempo Real de la Polimerasa/veterinaria , Análisis de Secuencia de ADN , SerotipificaciónRESUMEN
Control of foot-and-mouth disease (FMD) in Uganda by ring vaccination largely depends on costly trivalent vaccines, and use of monovalent vaccines could improve the cost effectiveness. This, however, requires application of highly specific diagnostic tests. This study investigated outbreaks of FMD in seven Ugandan districts, during 2011, using the PrioCHECK® FMDV NS ELISA, solid-phase blocking ELISAs (SPBEs) and virus neutralization tests (VNTs), together with virological analyses for characterization of the responsible viruses. Two hundred and eighteen (218) cattle and 23 goat sera as well as 82 oropharyngeal fluid/epithelial tissue samples were collected. Some 50% of the cattle and 17% of the goat sera were positive by the PrioCHECK® FMDV NS ELISA, while SPBEs identified titres ≥80 for antibodies against serotype O FMD virus (FMDV) in 51% of the anti-NSP positive cattle sera. However, 35% of the anti-NSP positive cattle sera had SPBE titres ≥80 against multiple serotypes, primarily against serotypes O, SAT 1 and SAT 3. Comparison of SPBEs and VNTs for the detection of antibodies against serotypes O, SAT 1 and SAT 3 in 72 NSP positive cattle sera showed comparable results against serotype O (P = 0.181), while VNTs detected significantly fewer samples positive for antibodies against SAT 1 and SAT 3 than the SPBEs (P < 0.001). Detection of antibodies against serotype O was consistent with the isolation of serotype O FMDVs from 13 samples. Four of these viruses were sequenced and belonged to two distinct lineages within the East Africa-2 (EA-2) topotype, each differing from the currently used vaccine strain (EA-1 topotype). The relationships of these lineages to other serotype O viruses in the Eastern Africa region are discussed. To enhance the control of FMD in Uganda, there is need to improve the specificity of the SAT-SPBEs, perform vaccine matching and implement improved regional FMD control.
Asunto(s)
Brotes de Enfermedades/veterinaria , Virus de la Fiebre Aftosa/aislamiento & purificación , Fiebre Aftosa/epidemiología , Animales , Bovinos , Brotes de Enfermedades/prevención & control , Ensayo de Inmunoadsorción Enzimática/veterinaria , Fiebre Aftosa/microbiología , Virus de la Fiebre Aftosa/clasificación , Virus de la Fiebre Aftosa/genética , Virus de la Fiebre Aftosa/inmunología , Cabras , Datos de Secuencia Molecular , Filogenia , Serogrupo , Uganda/epidemiología , Vacunación/veterinariaRESUMEN
Uganda had an unusually large number of foot-and-mouth disease (FMD) outbreaks in 2006, and all clinical reports were in cattle. A serological investigation was carried out to confirm circulating antibodies against foot-and-mouth disease virus (FMDV) by ELISA for antibodies against non-structural proteins and structural proteins. Three hundred and forty-nine cattle sera were collected from seven districts in Uganda, and 65% of these were found positive for antibodies against the non-structural proteins of FMDV. A subset of these samples were analysed for serotype specificity of the identified antibodies. High prevalences of antibodies against non-structural proteins and structural proteins of FMDV serotype O were demonstrated in herds with typical visible clinical signs of FMD, while prevalences were low in herds without clinical signs of FMD. Antibody titres were higher against serotype O than against serotypes SAT 1, SAT 2 and SAT 3 in the sera investigated for serotype-specific antibodies. Only FMDV serotype O virus was isolated from one probang sample. This study shows that the majority of the FMD outbreaks in 2006 in the region studied were caused by FMDV serotype O; however, there was also evidence of antibodies to both SAT 1 and SAT 3 in one outbreak in a herd inside Queen Elizabeth national park area.
Asunto(s)
Enfermedades de los Bovinos/virología , Virus de la Fiebre Aftosa/inmunología , Fiebre Aftosa/inmunología , Animales , Bovinos , Enfermedades de los Bovinos/epidemiología , Fiebre Aftosa/epidemiología , Fiebre Aftosa/virología , Uganda/epidemiologíaRESUMEN
In East Africa, the foot-and-mouth disease (FMD) virus (FMDV) isolates have over time included serotypes O, A, C, Southern African Territories (SAT) 1 and SAT 2, mainly from livestock. SAT 3 has only been isolated in a few cases and only in African buffalos (Syncerus caffer). To investigate the presence of antibodies against FMDV serotypes in wildlife in Uganda, serological studies were performed on buffalo serum samples collected between 2001 and 2003. Thirty-eight samples from African buffalos collected from Lake Mburo, Kidepo Valley, Murchison Falls and Queen Elizabeth National Parks were screened using Ceditest FMDV NS to detect antibodies against FMDV non-structural proteins (NSP). The seroprevalence of antibodies against non-structural proteins was 74%. To characterize FMDV antibodies, samples were selected and titrated using serotype-specific solid phase blocking enzyme linked immunosorbent assay (ELISAs). High titres of antibodies (> or =1 : 160) against FMDV serotypes SAT 1, SAT 2 and SAT 3 were identified. This study suggests that African buffalos in the different national parks in Uganda may play an important role in the epidemiology of SAT serotypes of FMDV.
Asunto(s)
Anticuerpos Antivirales/sangre , Búfalos , Virus de la Fiebre Aftosa/inmunología , Fiebre Aftosa/epidemiología , Animales , Fiebre Aftosa/inmunología , Virus de la Fiebre Aftosa/clasificación , Estudios Seroepidemiológicos , Serotipificación/veterinaria , Uganda/epidemiologíaRESUMEN
Foot-and-mouth disease (FMD) is endemic in Uganda with control strategies focusing on vaccination of cattle, while small ruminants are largely ignored. In order for Uganda to establish effective control strategies, it is crucial that the epidemiology of the disease is fully understood. This study summarizes results of serological investigations of sheep and goats for antibodies to FMDV from four districts in 2006 following an FMD outbreak in the region and from an attempted comprehensive random sampling in two districts in 2007. Antibodies were quantified and serotyped using competitive ELISA for antibodies towards non-structural proteins (NSP) and structural proteins towards serotype O, and blocking ELISA for antibodies towards the seven serotypes of FMD virus (FMDV). In 2006, sheep and goats in Bushenyi and Isingiro districts were free from antibodies towards FMDV, while herds in Kasese and Mbarara districts excluding Kahendero village were all positive for antibodies towards NSP and SP-O. In 2007, mean prevalence estimates of antibodies towards FMDV NSP was 14% in goats and 22% in sheep in Kasese district, while Bushenyi was still free. The difference between these two districts probably reflects different levels of FMDV challenge attributed to the variation in exposure rates which again in part may be as a result of the differing husbandry practices. Contrary to 2006, with clear antibodies towards serotype O, the serotype-specificity of the antibodies was less clear in 2007, as antibodies towards both serotype O and SAT serotypes were identified. Our results show that goats and sheep are infected during FMD outbreaks, and that they may be useful for determining the serotype of FMD outbreaks in Uganda, if they are sampled shortly after an outbreak.
Asunto(s)
Anticuerpos Antivirales/sangre , Virus de la Fiebre Aftosa/inmunología , Fiebre Aftosa/inmunología , Animales , Brotes de Enfermedades/veterinaria , Ensayo de Inmunoadsorción Enzimática/veterinaria , Fiebre Aftosa/epidemiología , Cabras , Prevalencia , Ovinos , Uganda/epidemiología , Proteínas no Estructurales Virales/inmunologíaRESUMEN
In 1998 and 2002, European bat lyssavirus type-1 (EBLV-1) was demonstrated in brain tissue of five Danish sheep suffering from neurological disorders. Four of the five sheep also had encephalic listeriosis. The animals originated from four flocks on pastures within a limited area of western Jutland. In a serological investigation in two of the herds, from which three of the diseased animals originated, EBLV-1 neutralizing antibodies were detected in only one of 69 sheep. In follow-up surveys, 2110 sheep sera collected at Danish slaughterhouses during 2000 were all negative for EBLV-1-antibodies, and EBLV-1 was not demonstrated in 87 ruminants displaying neurological symptoms. To investigate the pathogenic effects of EBLV-1, four sheep were inoculated intralabially with either brain material from one of the naturally infected sheep or virus isolated from the same sheep. These animals developed EBLV-1 neutralizing antibodies at 5-9 weeks post-inoculation but did not exhibit neurological signs during a 33-week observation period. It was speculated that the immune response prevented viral dissemination to the brain, resulting in an abortive peripheral infection. It was concluded that EBLV-1 can infect sheep under natural conditions as an incidental event.
Asunto(s)
Quirópteros/virología , Lyssavirus/patogenicidad , Infecciones por Rhabdoviridae/veterinaria , Enfermedades de las Ovejas/patología , Animales , Anticuerpos Antivirales/análisis , Encéfalo/patología , Encéfalo/virología , Dinamarca , Susceptibilidad a Enfermedades , Lyssavirus/inmunología , Lyssavirus/aislamiento & purificación , Tamizaje Masivo/veterinaria , Ratones , Ratones Endogámicos BALB C , ARN Viral/análisis , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/veterinaria , Infecciones por Rhabdoviridae/diagnóstico , Infecciones por Rhabdoviridae/transmisión , Infecciones por Rhabdoviridae/virología , Ovinos , Enfermedades de las Ovejas/virologíaAsunto(s)
Anticuerpos Antivirales/sangre , Enfermedades de los Gatos/epidemiología , Infecciones por Rhabdoviridae/veterinaria , Animales , Gatos , Dinamarca/epidemiología , Lyssavirus/inmunología , Infecciones por Rhabdoviridae/epidemiología , Estudios Seroepidemiológicos , Especificidad de la EspecieRESUMEN
We have isolated from calf serum a protein with an apparent M(r) of 120,000. The protein was detected by using antibodies against major acute-phase protein in pigs with acute inflammation. The amino acid sequence of an internal fragment revealed that this protein is the bovine counterpart of ITIH4, the heavy chain 4 of the inter-alpha-trypsin inhibitor family. The response of this protein in the sera was determined for animals during experimental bacterial and viral infections. In the bacterial model, animals were inoculated with a mixture of Actinomyces pyogenes, Fusobacterium necrophorum, and Peptostreptococcus indolicus to induce an acute-phase reaction. All animals developed moderate to severe clinical mastitis and exhibited remarkable increases in ITIH4 concentration in serum (from 3 to 12 times the initial values, peaking at 48 to 72 h after infection) that correlated with the severity of the disease. Animals with experimental infections with bovine respiratory syncytial virus (BRSV) also showed increases in ITIH4 concentration (from two- to fivefold), which peaked at around 7 to 8 days after inoculation. Generally, no response was seen after a second infection of the same animals with the virus. Because of the significant induction of the protein in the animals in the mastitis and BRSV infection models, we can conclude that ITIH4 is a new positive acute-phase protein in cattle.
Asunto(s)
Proteínas de Unión al Calcio/aislamiento & purificación , Bovinos/metabolismo , Glicoproteínas/aislamiento & purificación , Infecciones/metabolismo , Secuencia de Aminoácidos , Animales , Proteínas de Unión al Calcio/metabolismo , Electroforesis en Gel de Poliacrilamida , Femenino , Glicoproteínas/metabolismo , Mastitis Bovina/metabolismo , Proteínas Inhibidoras de Proteinasas SecretorasRESUMEN
European bat lyssavirus type 1 (EBLV-1, genotype 5) is known to endemically circulate in insectivorous bat populations in Germany. In August 2001, a rabies suspect stone marten (Martes foina) was found in the city of Burg (Saxony-Anhalt, Germany) and was sent to the regional veterinary laboratory for routine rabies diagnosis. Whereas brain samples repeatedly tested negative in the fluorescent antibody test for classical rabies virus (genotype 1), the mouse inoculation test and the rabies tissue culture inoculation test yielded positive results. Rabies viral RNA was also detected in the stone marten brain sample both by nested and heminested RT-PCR specific for the nucleoprotein gene and for the nucleoprotein phosphoprotein junction of rabies virus. The amplification products were sequenced to genotype the isolate. Sequence data obtained from the first-round RT-PCR products were analysed and the suspect stone marten isolate was confirmed as a rabies related virus (EBLV-1a). Phylogenetic comparison with sequences from recent genotype five isolates from Germany and Denmark showed that it was closely related to a previous isolate of EBLV-1 from a serotine bat in Saxony-Anhalt obtained in the same year in an area adjacent to the place where the EBLV-1 infected stone marten was found. Both EBLV-1 isolates share a 99.5% identity. This is the first report of an EBLV-1a spill-over from an insectivorous bat into wildlife in Europe.
Asunto(s)
Carnívoros , Lyssavirus/aislamiento & purificación , Infecciones por Rhabdoviridae/veterinaria , Animales , Secuencia de Bases , ADN Viral/análisis , Diagnóstico Diferencial , Alemania , Lyssavirus/genética , Datos de Secuencia Molecular , Filogenia , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/veterinaria , Infecciones por Rhabdoviridae/virologíaRESUMEN
Bovine respiratory syncytial virus (BRSV) has been recognised as an important pathogen in calf pneumonia for 30 years, but surprisingly few effective infection models for studies of the immune response and the pathogenesis in the natural host have been established. We present a reproducible experimental infection model for BRSV in 2-5-month-old, conventionally reared Jersey calves. Thirty-four colostrum-fed calves were inoculated once by aerosol and intratracheal injection with BRSV. Respiratory disease was recorded in 91% of the BRSV-inoculated calves, 72% had an accompanying rise in rectal temperature and 83% exhibited >5% consolidation of the lung tissue. The disease closely resembled natural outbreaks of BRSV-related pneumonia, and detection of BRSV in nasal secretions and lung tissues confirmed the primary role of BRSV. Nine mock-inoculated control calves failed to develop respiratory disease. This model is a valuable tool for the study of the pathogenesis of BRSV and for vaccine efficacy studies.
Asunto(s)
Enfermedades de los Bovinos/virología , Bovinos/virología , Modelos Animales de Enfermedad , Neumonía/veterinaria , Neumonía/virología , Virus Sincitial Respiratorio Bovino/fisiología , Animales , Anticuerpos Antivirales/sangre , Antígenos Virales/aislamiento & purificación , Enfermedades de los Bovinos/inmunología , Enfermedades de los Bovinos/patología , Pulmón/inmunología , Pulmón/patología , Neumonía/inmunología , Neumonía/patología , Virus Sincitial Respiratorio Bovino/inmunología , Factores de TiempoRESUMEN
The ability of a pure virus infection to induce an acute phase protein response is of interest as viral infections are normally considered to be less efficient in inducing an acute phase protein response than bacterial infections. This was studied in a bovine model for infection with bovine respiratory syncytial virus (BRSV), analysing the induction of the two most dominant bovine acute phase proteins haptoglobin and serum amyloid A (SAA). Strong and reproducible acute phase responses were detected for both proteins, peaking at around 7-8 days after inoculation of BRSV, while no response was seen in mock-inoculated control animals. The serum concentrations reached for SAA and haptoglobin during the BRSV-induced acute phase response were generally the same or higher than previously reported for bacterial infections in calves. The magnitude and the duration of the haptoglobin response was found to correlate well with the severity of clinical signs (fever) and with the extent of lung consolidation while SAA responded most rapidly to infection.
Asunto(s)
Reacción de Fase Aguda/veterinaria , Apolipoproteínas/metabolismo , Enfermedades de los Bovinos/sangre , Haptoglobinas/metabolismo , Infecciones por Virus Sincitial Respiratorio/veterinaria , Virus Sincitial Respiratorio Bovino , Proteína Amiloide A Sérica/metabolismo , Reacción de Fase Aguda/sangre , Reacción de Fase Aguda/inmunología , Animales , Bovinos , Enfermedades de los Bovinos/inmunología , Masculino , Infecciones por Virus Sincitial Respiratorio/sangre , Infecciones por Virus Sincitial Respiratorio/inmunología , Factores de TiempoRESUMEN
The nucleotides coding for the extracellular part of the G glycoprotein and the full SH protein of bovine respiratory syncytial virus (BRSV) were sequenced from viruses isolated from numerous outbreaks of BRSV infection. The isolates included viruses isolated from the same herd (closed dairy farms and veal calf production units) in different years and from all confirmed outbreaks in Denmark within a short period. The results showed that identical viruses were isolated within a herd during outbreaks and that viruses from recurrent infections varied by up to 11% in sequence even in closed herds. It is possible that a quasispecies variant swarm of BRSV persisted in some of the calves in each herd and that a new and different highly fit virus type (master and consensus sequence) became dominant and spread from a single animal in connection with each new outbreak. Based on the high level of diversity, however, the most likely explanation was that BRSV was (re)introduced into the herd prior to each new outbreak. These findings are highly relevant for the understanding of the transmission patterns of BRSV among calves and human respiratory syncytial virus among humans.
Asunto(s)
Enfermedades de los Bovinos/epidemiología , Brotes de Enfermedades/veterinaria , Variación Genética , Infecciones por Virus Sincitial Respiratorio/veterinaria , Virus Sincitial Respiratorio Bovino/genética , Virus Sincitial Respiratorio Bovino/aislamiento & purificación , Animales , Bovinos , Enfermedades de los Bovinos/virología , Dinamarca/epidemiología , Datos de Secuencia Molecular , Filogenia , Recurrencia , Infecciones por Virus Sincitial Respiratorio/epidemiología , Infecciones por Virus Sincitial Respiratorio/virología , Análisis de Secuencia de ADN , Proteínas Virales/genéticaRESUMEN
Bovine respiratory syncytial virus (BRSV) is an important cause of respiratory disease among calves in the Danish cattle industry. An experimental BRSV infection model was used to study the pathogenesis of the disease in calves. Broncho alveolar lung lavage (BAL) was performed on 28 Jersey calves, of which 23 were experimentally infected with BRSV and five were given a mock inoculum. The presence of the cytokine tumor necrosis factor alpha (TNF-alpha) in the BAL fluids was detected and quantified by a capture ELISA. TNF-alpha was detected in 21 of the infected animals. The amount of TNF-alpha in the BAL fluid of calves killed post inoculation day (PID) 2 and 4 was at the same very low level as in the uninfected control animals. Large amounts of TNF-alpha were detected on PID 6, maximum levels of TNF-alpha were reached on PID 7, and smaller amounts of TNF-alpha were seen on PID 8. The high levels of TNF-alpha appeared on the days where severe lung lesions and clinical signs were obvious and the amounts of BRSV-antigen were at their greatest. Although Pasteurellaceae were isolated from some of the BRSV-infected calves, calves treated with antibiotics before and through the whole period of the infection, as well as BRSV-infected calves free of bacteria reached the same level of TNF-alpha as animals from which bacteria were isolated from the lungs. It is concluded that significant quantities of TNF-alpha are produced in the lungs of the calves on PID 6-7 of BRSV infection. The involvement of TNF-alpha in the pathogenesis of, as well as the anti-viral immune response against, BRSV infection is discussed.
Asunto(s)
Enfermedades de los Bovinos/inmunología , Fluoroquinolonas , Pulmón/metabolismo , Infecciones por Virus Sincitial Respiratorio/veterinaria , Virus Sincitial Respiratorio Bovino/inmunología , Factor de Necrosis Tumoral alfa/metabolismo , Animales , Antiinfecciosos/uso terapéutico , Antígenos Virales/análisis , Líquido del Lavado Bronquioalveolar/inmunología , Líquido del Lavado Bronquioalveolar/virología , Bovinos , Enfermedades de los Bovinos/fisiopatología , Enfermedades de los Bovinos/virología , Enrofloxacina , Ensayo de Inmunoadsorción Enzimática/veterinaria , Inmunohistoquímica/veterinaria , Pulmón/patología , Pulmón/virología , Masculino , Quinolonas/uso terapéutico , Infecciones por Virus Sincitial Respiratorio/inmunología , Infecciones por Virus Sincitial Respiratorio/fisiopatología , Infecciones por Virus Sincitial Respiratorio/virología , Factor de Necrosis Tumoral alfa/análisis , Factor de Necrosis Tumoral alfa/biosíntesisRESUMEN
Using specific ELISAs, antibody levels of four different isotypes to bovine respiratory syncytial virus (BRSV) were determined in calves, following experimental BRSV infection. Most calves experienced an increase in the specific IgM and IgG1 titres about 6-10 days after infection with BRSV. The IgM titre was transient showing positive titres for only 5-10 days, while specific IgG1 was present for a longer time. IgA was detected concomitantly with IgM but at a lower level. Production of IgG2 anti-BRSV antibodies was detected from 3 weeks after infection. In two closed herds, repeated blood samplings were performed on young stock to analyse maternal immunity. The passively transferred antibodies were mainly of the IgG1 isotype and the half-life of IgG1 to BRSV was estimated to be 26.6 days. One of the herds had an outbreak of enzootic pneumonia, diagnosed to be caused by BRSV. Furthermore, another herd with acute BRSV was followed by weekly blood samples in six calves; in both herds IgM and IgG1 was detected shortly after the appearance of clinical signs. Serum samples from 50 Danish dairy herds (453 samples) were tested for immunoglobulins of the isotypes IgG1, IgG2 and IgM. The presence of antibodies to BRSV was widespread and more than 54% of the samples had BRSV antibodies of both the IgG1 and IgG2 isotypes indicating a high herd prevalence to BRSV. Test samples from two herds out of 50 were free from all isotypes to BRSV.
Asunto(s)
Anticuerpos Antivirales/análisis , Enfermedades de los Bovinos/virología , Isotipos de Inmunoglobulinas/análisis , Infecciones por Virus Sincitial Respiratorio/veterinaria , Virus Sincitial Respiratorio Bovino/inmunología , Animales , Bovinos , Enfermedades de los Bovinos/epidemiología , Enfermedades de los Bovinos/inmunología , Dinamarca/epidemiología , Brotes de Enfermedades/veterinaria , Ensayo de Inmunoadsorción Enzimática/veterinaria , Femenino , Inmunidad Materno-Adquirida , Masculino , Infecciones por Virus Sincitial Respiratorio/epidemiología , Infecciones por Virus Sincitial Respiratorio/inmunología , Estudios SeroepidemiológicosRESUMEN
One hundred Danish dairy calves had temperature loggers implanted subcutaneously on the neck. Post-operatively, the calves were given a single antibiotic treatment, and tissue reactions were assessed on 6 post-operative visits. After approximately 5 months, the loggers were removed and material submitted for histologic examination. This paper presents 1) the surgical procedure, 2) the prevalence of tissue reaction at the post-operative visits, 3) the degree of implant recovery, 4) the results of histopathologic examinations, 5) an evaluation of age at implantation or veterinary practitioner as risk factors for tissue reaction and missing implant recovery 5 months after implantation, and 6) evaluation of tissue reaction as a risk factor for lack of recovery 5 months after implantation. The implant was rejected on 7 calves (7%). Additionally, 5 calves (5%) had the temperature logger removed because of presence of an abcess. No migration of the temperature loggers were observed. The results of a repeated measures analysis and the histopathological findings indicate that contamination during the surgery resulted in inflammation and abcess formation. It is recommended that in the presence of an abcess, the temperature logger should be removed.
Asunto(s)
Bovinos/cirugía , Tejido Conectivo/patología , Termómetros/veterinaria , Factores de Edad , Animales , Temperatura Corporal , Bovinos/fisiología , Femenino , Cuello/cirugía , Análisis de Regresión , VeterinariosRESUMEN
A reverse transcription-polymerase chain reaction (RT-PCR) assay was developed for detection of bovine respiratory syncytial virus (BRSV) in lung tissue of naturally and experimentally infected cattle. Primers were selected from the gene coding the F fusion protein, which is relatively conserved among BRSV isolates. The RT-PCR assay was highly specific, it yielded positive reactions only when performed on BRSV-infected cell cultures or tissues. The detection limit of the RT-PCR assay was assessed as 5 TCID50. BRSV was detected in tissues of the respiratory tract and in the tracheobroncheal lymph node of calves euthanized 2-8 days after experimental infection with BRSV, whereas samples of other tissues and samples from mock-infected animals were negative at all time points. Examination of lung samples from 8 different regions of the lungs revealed that although the virus was most often found in the cranioventral lobules, it was frequently present in all lung lobules. Microbiologic examinations of all acute fatal cases of pneumonia (135 animals) in cattle submitted for diagnostic purposes during 1 year revealed that Actinomyces pyogenes (11%), Haemophilus somnus (10%), Pasteurella sp. (7%), and Pasteurella haemolytica (7%) were the most common bacterial agents found in the lungs. BRSV was identified using a conventional antigen enzyme-linked immunosorbent assay (ELISA) in 23 (17%) animals. The established BRSV-specific RT-PCR assay yielded positive results for the same 23 animals. In addition, 10 animals that were negative with the ELISA were positive with the RT-PCR assay. These results indicates that the RT-PCR assay can be a sensitive, reliable alternative to conventional diagnostic procedures.
Asunto(s)
ADN Viral/análisis , Neumonía Viral/veterinaria , Infecciones por Virus Sincitial Respiratorio/diagnóstico , Virus Sincitial Respiratorio Bovino/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/veterinaria , Animales , Bovinos , Dinamarca/epidemiología , Neumonía Viral/diagnóstico , Infecciones por Virus Sincitial Respiratorio/genética , Virus Sincitial Respiratorio Bovino/patogenicidad , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Sensibilidad y EspecificidadRESUMEN
Danish isolates of bovine respiratory syncytial virus (BRSV) were characterised by nucleotide sequencing of the G glycoprotein and by their reactivity with a panel of monoclonal antibodies (MAbs). Among the six Danish isolates, the overall sequence divergence ranged between 0 and 3% at the nucleotide level and between 0 and 5% at the amino acid level. Sequence divergences of 7-8%, 8-9% and 2-3% (nucleotide) and 9-11%, 12-16% and 4-6% (amino acid) were obtained in the comparison made between the group of Danish isolates and the previously sequenced 391-2USA, 127UK and 220-69Bel isolates, respectively. Phylogenetic analysis showed that the Danish isolates formed three lineages within a separate branch of the phylogenetic tree. Nevertheless, the Danish isolates were closely related to the 220-69Bel isolate, the prototype of the intermediate antigenic subgroup. The sequencing of the extracellular part of the G gene of additional 11 field BRSV viruses, processed directly from lung samples without prior adaption to cell culture growth, revealed sequence variabilities in the range obtained with the propagated virus. In addition, several passages in cell culture and in calves had no major impact on the nucleotide sequence of the G protein. These findings indicated that the previously established variabilities of the G protein of RS virus isolates were not attributable to mutations induced during the propagation of the virus. The reactivity of the Danish isolates with G protein-specific MAbs were similar to that of the 220-69Bel isolate. Furthermore, the sequence of the immunodominant region was completely conserved among the Danish isolates on one side and the 220-69Bel isolate on the other. When combined, these data strongly suggested that the Danish isolates belong to the intermediate subgroup.
Asunto(s)
Enfermedades de los Bovinos/virología , Infecciones por Virus Sincitial Respiratorio/veterinaria , Virus Sincitial Respiratorio Bovino/clasificación , Virus Sincitial Respiratorio Bovino/genética , Proteínas del Envoltorio Viral/genética , Secuencia de Aminoácidos , Animales , Anticuerpos Monoclonales , Antígenos Virales/genética , Secuencia de Bases , Bovinos , Células Cultivadas , Dinamarca , Variación Genética , Datos de Secuencia Molecular , Sistemas de Lectura Abierta , Filogenia , Reacción en Cadena de la Polimerasa/métodos , Infecciones por Virus Sincitial Respiratorio/virología , Virus Sincitial Respiratorio Bovino/aislamiento & purificación , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Homología de Secuencia de Ácido Nucleico , Proteínas del Envoltorio Viral/químicaRESUMEN
A field study was conducted in the Southern Region of Malawi to evaluate the possible benefits of immunisation of improved dairy cattle against Anaplasma marginale, Babesia bigemina and Babesia bovis. Friesian crossbred heifers were immunised when they were being reared on Government farms. They were then issued to smallholder farmers, together with unvaccinated controls, where many of them were exposed to heavy tick infestation. Vaccination was shown to provide a significant degree of protection against babesiosis on the smallholder farms; 15/32 unvaccinated controls developed clinical disease as compared to only 3/28 vaccinates.
Asunto(s)
Anaplasmosis/prevención & control , Babesiosis/prevención & control , Vacunas Bacterianas/normas , Enfermedades de los Bovinos/prevención & control , Vacunas Antiprotozoos/normas , Anaplasma/inmunología , Anaplasmosis/sangre , Anaplasmosis/epidemiología , Animales , Anticuerpos Antibacterianos/sangre , Anticuerpos Antiprotozoarios/sangre , Babesia/inmunología , Babesiosis/sangre , Babesiosis/epidemiología , Bovinos , Enfermedades de los Bovinos/epidemiología , Enfermedades de los Bovinos/parasitología , Industria Lechera/métodos , Femenino , Incidencia , Malaui/epidemiologíaRESUMEN
Crossbred dairy heifers on a farm in an East Coast fever (ECF) endemic area in Malawi were immunised against Theileria parva, Anaplasma spp., Babesia bigemina, Babesia bovis and Cowdria ruminantium. They were treated at infrequent intervals with chlorfenvinphos to limit infestation with adult ticks, without providing complete tick control. In one trial, which tested a threshold dipping regimen, 20 heifers were dipped only once in 6 months to control a flush of Boophilus microplus. Unimmunised controls showed serological evidence of exposure to T. parva and B. bigemina, and one died of ECF, but there were no incidents of tick-borne disease in the immunised group. In a second trial, which tested a strategic dipping regimen, 107 animals were dipped 9 times over a 6 month period. Despite heavy challenge by B. bovis and moderate challenge by B. bigemina and Anaplasma spp. demonstrated serologically, there was only a single clinical case of babesiosis. The observations provide encouragement for the introduction of integrated tick and tick-borne disease control programmes in improved cattle in ECF endemic areas.
Asunto(s)
Enfermedades de los Bovinos/prevención & control , Control de Ácaros y Garrapatas/métodos , Infestaciones por Garrapatas/veterinaria , Enfermedades por Picaduras de Garrapatas/veterinaria , Administración Tópica , Anaplasmosis/prevención & control , Animales , Babesia bovis/inmunología , Babesiosis/prevención & control , Bovinos , Clorfenvinfos/administración & dosificación , Clorfenvinfos/uso terapéutico , Cruzamientos Genéticos , Ehrlichia ruminantium/inmunología , Femenino , Hidropericardio/prevención & control , Inmunización/veterinaria , Insecticidas/administración & dosificación , Insecticidas/uso terapéutico , Malaui , Masculino , Theileria parva/inmunología , Theileriosis/prevención & control , Infestaciones por Garrapatas/prevención & control , Enfermedades por Picaduras de Garrapatas/prevención & controlRESUMEN
In an outbreak of Babesia bovis in a large herd of Friesian x Malawi Zebu cattle, which occurred after an interruption of intensive dipping, clinical or fatal babesiosis occurred in 54/299 (18.1%) animals which had never been vaccinated, as compared to 9/153 (5.9%) vaccinated animals. Eight of the nine affected vaccinates had been vaccinated more than 27 months previously. Sera were collected every 3-4 months from 33 Friesian x Malawi Zebu heifers maintained with intensive dipping and vaccinated with trivalent B. bovis, Babesia bigemina and Anaplasma centrale vaccine. After 2 years, 25% had become seronegative for B. bovis by indirect immunofluorescence, as compared to 97% for B. bigemina and 46% for A. centrale. Because of the evidence that immunity following vaccination against B. bovis declines after 2 years in the absence of tick challenge, it is recommended that tick control should be relaxed after immunity has been established, in order to save acaricide, reinforce immunity and avoid any need for revaccination.