Reverse electron transfer: Novel anaerobic methanogenesis pathway regulated through exogenous CO2 synergized with biochar.

Acid accumulation and carbon emission are two major challenges in anaerobic digestion. Syntrophic consortia can employ reverse electron transfer (RET) to facilitate thermodynamically unfavorable redox reactions during acetogenesis. However, the potential mechanisms and regulatory methods of RET remain unclear. This study examines the regulatory mechanisms by which exogenous CO2 affects RET and demonstrates that biochar maximizes CO2 solubility at 25.8 mmol/L to enhance effects further. CO2 synergized with biochar significantly increases cumulative methane production and propionate degradation rate. From the bioenergetic perspective, CO2 decreases energy level to a maximum of -87 kJ/mol, strengthening the thermodynamic viability. The underlying mechanism can be attributed to RET promotion, as indicated by increased formate dehydrogenase and enrichment of H2/formate-producing bacteria with their partner Methanospirillum hungatei. Moreover, the 5 % 13CH4 and methane contribution result show that CO2 accomplishes directed methanogenesis. Overall, this investigation riches the roles of CO2 and biochar in AD surrounding RET.

Enhancing sustainable crop cultivation: The impact of renewable soil amendments and digestate fertilizer on crop growth and nutrient composition.

Utilizing digestate as a fertilizer enhances soil nutrient content, improves fertility, and minimizes nutrient runoff, mitigating water pollution risks. This alternative approach replaces commercial fertilizers, thereby reducing their environmental impact and lowering greenhouse gas emissions associated with fertilizer production and landfilling. Herein, this study aimed to evaluate the impact of various soil amendments, including carbon fractions from waste materials (biochar, compost, and cocopeat), and food waste anaerobic digestate application methods on tomato plant growth (Solanum lycopersicum) and soil fertility. The results suggested that incorporating soil amendments (biochar, compost, and cocopeat) into the potting mix alongside digestate application significantly enhances crop yields, with increases ranging from 12.8 to 17.3% compared to treatments without digestate. Moreover, the combination of soil-biochar amendment and digestate application suggested notable improvements in nitrogen levels by 20.3% and phosphorus levels by 14%, surpassing the performance of the those without digestate. Microbial analysis revealed that the soil-biochar amendment significantly enhanced biological nitrification processes, leading to higher nitrogen levels compared to soil-compost and soil-cocopeat amendments, suggesting potential nitrogen availability enhancement within the rhizosphere's ecological system. Chlorophyll content analysis suggested a significant 6.91% increase with biochar and digestate inclusion in the soil, compared to the treatments without digestate. These findings underscore the substantial potential of crop cultivation using soil-biochar amendments in conjunction with organic fertilization through food waste anaerobic digestate, establishing a waste-to-food recycling system.

Enhanced thermophilic dark fermentation of hydrogen production from food waste by Fe-modified biochar.

The industrialization of hydrogen production through dark fermentation of food waste faces challenges, such as low yields and unpredictable fermentation processes. Biochar has emerged as a promising green additive to enhance hydrogen production in dark fermentation. Our study demonstrated that the introduction of Fe-modified biochar (Fe-L600) significantly boosted hydrogen production during thermophilic dark fermentation of food waste. The addition of Fe-L600 led to a remarkable 31.19% increase in hydrogen yield and shortened the time needed for achieving stabilization of hydrogen production from 18 h to 12 h. The metabolite analysis revealed an enhancement in the butyric acid pathway as the molar ratio of acetic acid to butyric acid decreased from 3.09 to 2.69 but hydrogen yield increased from 57.12 ± 1.48 to 76.78 ± 2.77 mL/g, indicating Fe-L600 improved hydrogen yield by regulating crucial metabolic pathways of hydrogen production. The addition of Fe-L600 also promoted the release of Fe2+ and Fe3+ and increased the concentrations of Fe2+ and Fe3+ in the fermentation system, which might promote the activity of hydrogenase and ferredoxin. Microbial community analysis indicated a substantial increase in the relative abundance of Thermoanaerobacterium after thermophilic dark fermentation. The relative abundances of microorganisms responsible for hydrolysis and acidogenesis were also observed to be improved in the system with Fe-L600 addition. This research provides a feasible strategy for improving hydrogen production of food waste and deepens the understanding of the mechanisms of biochar.

Life cycle assessment and cost-benefit analysis of small-scale anaerobic digestion system treating food waste onsite under different operational conditions.

This study employed life cycle assessment and cost-benefit analysis to evaluate the environmental and economic profile of a real decentralized small-scale anaerobic digestion (AD) system treating food waste (FW). Different operational conditions, including temperature, biochar addition, biogas engine efficiency, and FW loading, were compared via scenario analysis. Biochar addition could potentially obtain carbon reduction and save fossil fuel. Moreover, at high FW loading and biogas engine efficiency, biochar addition achieved 1-3190% better performance than the system without biochar in all the nine impact categories. The system under mesophilic conditions performed worse than ambient conditions due to high energy demand. All the current scenarios resulted in a monetary loss at US$ 480 k-681 k, while profit was possible if the capital cost and operator salary decreased significantly. Overall, operating the small-scale AD system under ambient temperature with biochar addition was preferred due to its potential environmental benefits and economic profits.

Improving urban ecosystem holistic sustainability of municipal solid waste-to-energy strategy using extended exergy accounting analysis.

Waste-to-energy technologies play a crucial role in integrated waste management strategies to reduce waste mass and volume, disinfect the waste, and recover energy; different technologies have advantages and disadvantages in treating municipal solid waste under urban conditions. This paper applies the extended exergy accounting method to develop an analytical framework to identify the optimal waste-to-energy strategy from an urban ecosystem holistic sustainability perspective. In the analytical framework, urban ecosystem costs and revenues are formulated as a multi-criteria cost-benefit quantitative model. The urban ecosystem cost is divided into five categories, and the urban ecosystem revenues consist of direct and indirect parts. The direct part is the chemical exergy of the waste-to-energy plants produced product, and the indirect part includes equivalent exergy content of power generation substitution, human health risk elimination, disamenity impact removal and environmental degradation avoidance. Proposing an indicator system to evaluate the waste-to-energy strategy impact on the sustainability of the urban ecosystems and social, economic and environmental sub-ecosystem. Detailed analysis of food waste treatment scenarios of a food center in Singapore was done as a case study to illustrate this analytical framework. Base scenario is current practice that food waste disposal in incineration plant. Anaerobic digestion and gasification are proposed as potential technological solutions for on-site food waste treatment in scenario I and II respectively. In different scenarios, the urban ecosystem costs are estimated to be 71,536.01, 61,854.87 and 74,190.34MJ/year respectively, and the urban ecosystem revenues are estimated to be 135,312.66, 405,442.53 and 298,426.81MJ/year respectively. We show that the scenario where food waste is treated by anaerobic digestion outperforms both the base scenario and scenario II in terms of urban ecosystem costs and revenues, technical energy conversion efficiency, contribution to urban ecosystem holistic sustainability, and natural, social, and economic subsystems improvement, making it the optimal municipal solid waste-to-energy strategy choice.

Synergetic anaerobic digestion of food waste for enhanced production of biogas and value-added products: strategies, challenges, and techno-economic analysis.

The generation of food waste (FW) is increasing at an alarming rate, contributing to a total of 32% of all the waste produced globally. Anaerobic digestion (AD) is an effective method for dealing with organic wastes of various compositions, like FW. Waste valorization into value-added products has increased due to the conversion of FW into biogas using AD technology. A variety of pathways are adopted by microbes to avoid unfavorable conditions in AD, including competition between sulfate-reducing bacteria and methane (CH4)-forming bacteria. Anaerobic bacteria decompose organic matter to produce biogas, a digester gas. The composition depends on the type of raw material and the method by which the digestion process is conducted. Studies have shown that the biogas produced by AD contains 65-75% CH4 and 35-45% carbon dioxide (CO2). Methanothrix soehngenii and Methanosaeta concilii are examples of species that convert acetate to CH4 and CO2. Methanobacterium bryantii, Methanobacterium thermoautotrophicum, and Methanobrevibacter arboriphilus are examples of species that produce CH4 from hydrogen and CO2. Methanobacterium formicicum, Methanobrevibacter smithii, and Methanococcus voltae are examples of species that consume formate, hydrogen, and CO2 and produce CH4. The popularity of AD has increased for the development of biorefinery because it is seen as a more environmentally acceptable alternative in comparison to physico-chemical techniques for resource and energy recovery. The review examines the possibility of using accessible FW to produce important value-added products such as organic acids (acetate/butyrate), biopolymers, and other essential value-added products.

HighlightsPopulation growth globally increases the generation of FW.FW generation, recycling, and reuse have been discussed.Biogas and bio-fertilizers can be recovered from FW through AD.

A closed loop case study of decentralized food waste management: System performance and life cycle carbon emission assessment.

Food waste (FW) has become a worldwide issue, while anaerobic digestion (AD) has appeared as a widely adopted technology to recover energy and resources from FW. Compared to many existing case studies of centralized AD system, the comprehensive study of decentralized micro-AD system from both system energy efficiency and carbon emission perspective is still scanty, particularly system operated under ambient temperature conditions. In this study, an actual decentralized micro-AD system with treating capacity of 300 kg FW/d for a local hawker center in Singapore was reported and evaluated. The results showed that 1894.5 kg of FW was treated and 173 m3 biogas with methane content of 53 % was produced during the experimental period of 75 days. The methane yield results showed a high FW degradation efficiency (87.87 %). However, net energy consumption and net carbon emission were observed during the experimental period. Nevertheless, energy self-efficiency and carbon neutrality, even net energy output and carbon reduction, can be achieved by increasing daily FW loading and biogas engine efficiency. Specifically, the FW loading for system energy self-efficiency was identified as 159 kg/d for engine efficiency of 35 % at a high kitchen waste/table waste ratio (63 %/37 %, with covid-19 dine-in restrictions); while they were 112 and 58 kg/d for engine efficiency of 25 % and 35 %, respective, at a low kitchen waste/table waste ratio (31 %/69 %, without covid-19 dine-in restrictions). The carbon emission ranged from 156.08 kg CO2-eq/t FW to -77.35 kg CO2-eq/t FW depending on the FW loading quantity and engine efficiency. Moreover, the sensitivity analysis also showed that the used electricity source for substitution influenced the carbon emission performance significantly. The obtained results imply that the decentralized micro-AD system could be a feasible FW management solution for energy generation and carbon reduction when the FW loading and engine electrical efficiency are carefully addressed.

Current insight into enhanced strategies and interaction mechanisms of hydrogel materials for phosphate removal and recovery from wastewater.

Phosphorus plays a crucial role in modern society but often pollutes the environment through raising eutrophication and has particularly devastating effects on the water environment. As a promising material platform, the three-dimensional network structure and the tailorable nature of hydrogels provide infinite application possibilities. Thereinto, phosphate removal and recovery from wastewater using hydrogel materials have gained momentum since their rapid reactivity, ease of operation, low cost and simplicity of recovery compared to traditional techniques. In this review, current strategies for functional enhancement of hydrogel materials are systematically summarized from different perspectives. Following, based on the discussion of different interaction mechanisms between phosphates and hydrogels, the phosphate mass transfer and performance of hydrogels and their current application are critically reviewed. This review aims to present mechanistic insight into the recent development in phosphate removal and recovery using hydrogel materials and provides new ideas for constructing high-efficient hydrogels and laying the foundations for the practical application of this technology.

Interfacial Polyelectrolyte Complexation-Inspired Bioprinting of Vascular Constructs.

Bioprinting is a precise layer-by-layer manufacturing technology utilizing biomaterials, cells, and sometimes growth factors for the fabrication of customized three-dimensional (3D) biological constructs. In recent years, it has gained considerable interest in various biomedical studies. However, the translational application of bioprinting is currently impeded by the lack in efficient techniques for blood vessel fabrications. In this report, by systematically studying the previously reported phenomenon, interfacial polyelectrolyte complexation, an efficient blood vessel bioprinting technique based on the phenomenon, was proposed and subsequently investigated. In this technique, anionic hyaluronate and cationic lysine-based peptide amphiphiles were placed concentrically to bioprint with human umbilical endothelial cells for the fabrication of biological tubular constructs. These constructs demonstrated clear vascular features, which made them highly resemble blood vessels. In addition, to optimize the bioactivity of the printed constructs, this report also, for the first time, studied peptide sequencing's effect on the biocompatibility of the polyelectrolyte-peptide amphiphile complex. All these studies conducted in the report are highly relevant and interesting for research in vascular structure fabrication, which will eventually be beneficial for translational application development of bioprinting.

Effects of digestate-encapsulated biochar on plant growth, soil microbiome and nitrogen leaching.

The increasing amount of food waste and the excessive use of mineral fertilizers have caused detrimental impacts on soil, water, and air quality. Though digestate derived from food waste has been reported to partially replace fertilizer, its efficiency requires further improvement. In this study, the effects of digestate-encapsulated biochar were comprehensively investigated based on growth of an ornamental plant, soil characteristics, nutrient leaching and soil microbiome. Results showed that except for biochar, the tested fertilizers and soil additives, i.e., digestate, compost, commercial fertilizer, digestate-encapsulated biochar had positive effects on plants. Especially, the digestate-encapsulated biochar had the best effectiveness as evidenced by 9-25% increase in chlorophyll content index, fresh weight, leaf area and blossom frequency. For the effects of fertilizers or soil additives on soil characteristics and nutrient retention, the digestate-encapsulated biochar leached least N-nutrients (<8%), while the compost, digestate and mineral fertilizer leached up to 25% N-nutrients. All the treatments had minimal effects on the soil properties of pH and electrical conductivity. According to the microbial analysis, the digestate-encapsulated biochar has the comparable role with compost in improving the soil immune system against pathogen infection. The metagenomics coupling with qPCR analysis suggested that digestate-encapsulated biochar boosted the nitrification process and inhibited the denitrification process. This study provides an extensive understanding into the impacts of the digestate-encapsulated biochar on an ornamental plant and offers practical implications for the choice of sustainable fertilizers or soil additives and food-waste digestate management.

Chromium toxicity and tolerance mechanisms in plants through cross-talk of secondary messengers: An overview of pathways and mechanisms.

Environmental sources of chromium (Cr) such as solid waste, battery chemicals, industrial /waste, automotive exhaust emissions, mineral mining, fertilizers, and pesticides, have detrimental effects on plants. An excessive amount of Cr exposure can lead to toxic accumulations in human, animal, and plant tissues. In plants, diverse signaling molecules like hydrogen sulfide (H2S) and nitric oxide (NO) play multiple roles during Cr stress. Consequently, the molecular mechanisms of Cr toxicity in plants, such as metal binding, modifying enzyme activity, and damaging cells are examined by several studies. The reactive oxygen species (ROS) that are formed when Cr reacts with lipids, membranes, DNA, proteins, and carbohydrates are all responsible for damage caused by Cr. ROS regulate plant growth, programmed cell death (PCD), cell cycle, pathogen defense, systemic communication, abiotic stress responses, and growth. Plants accumulate Cr mostly through the root system, with very little movement to the shoots. The characterization of stress-inducible proteins and metabolites involved in Cr tolerance and cross-talk messengers has been made possible due to recent advances in metabolomics, transcriptomics, and proteomics. This review discusses Cr absorption, translocation, subcellular distribution, and cross-talk between secondary messengers as mechanisms responsible for Cr toxicity and tolerance in plants. To mitigate this problem, soil-plant systems need to be monitored for the biogeochemical behavior of Cr and the identification of secondary messengers in plants.

Startup performance and microbial communities of a decentralized anaerobic digestion of food waste.

The aim of this work was to evaluate the feasibility of treating food waste generated from a hawker centre in a pilot-scale anaerobic digester operating on site in an urban area of Singapore. For this purpose, a 10.4 m3 digester was housed within two 20 feet containerized systems and sited adjacent to the hawker centre. The system reported in this work was during the startup phase, for over 71 days of real and varying food wastes loading rate. The results demonstrated that the decentralized system had an average specific methane yield of 0.55 ± 0.04 m3CH4/kgVS, with methane concentrations of 56.6 ± 2.3%. For the power generation output, the energy assessment revealed an average of 2.05 ± 0.57 m3/kW h consumption rate. Accordingly, on the last day of startup phase, the inoculum of the digester was richer in organisms from the phylum Thermoplasmatota, i.e., genera Candidatus_Methanogranum and Candidatus_Methanoplasma, alongside with other dominant abundance from phyla Bacteroidota, Firmicutes, Synergistota, and Verrucomicrobiota. This study provides new insights into pilot scale decentralized anaerobic digestion with varying food waste relate to the characterizations of digester microbial communities, as well as turning in a typical integrated anaerobic digestion of food waste-to-energy system a reality.

Machine learning and circular bioeconomy: Building new resource efficiency from diverse waste streams.

Biorefinery systems are playing pivotal roles in the technological support of resource efficiency for circular bioeconomy. Meanwhile, artificial intelligence presents great potential in handling scientific tasks of high-dimensional complexity. This review article scrutinizes the status of machine learning (ML) applications in four critical biorefinery systems (i.e. composting, fermentation, anaerobic digestion, and thermochemical conversions) as well as their advancements against traditional modeling techniques of mechanistic approach. The contents cover their algorithm selections, modeling challenges, and prospective improvements. Perspectives are sketched to further inform collective efforts on crucial aspects. The multidisciplinary interchange of modeling knowledge will enable a more progressive digital transformation of sustainability efforts in supporting sustainable development goals.

A Review on Enhancing Cupriavidus necator Fermentation for Poly(3-hydroxybutyrate) (PHB) Production From Low-Cost Carbon Sources.

In the context of a circular economy, bioplastic production using biodegradable materials such as poly(3-hydroxybutyrate) (PHB) has been proposed as a promising solution to fundamentally solve the disposal issue of plastic waste. PHB production techniques through fermentation of PHB-accumulating microbes such as Cupriavidus necator have been revolutionized over the past several years with the development of new strategies such as metabolic engineering. This review comprehensively summarizes the latest PHB production technologies via Cupriavidus necator fermentation. The mechanism of the biosynthesis pathway for PHB production was first assessed. PHB production efficiencies of common carbon sources, including food waste, lignocellulosic materials, glycerol, and carbon dioxide, were then summarized and critically analyzed. The key findings in enhancing strategies for PHB production in recent years, including pre-treatment methods, nutrient limitations, feeding optimization strategies, and metabolism engineering strategies, were summarized. Furthermore, technical challenges and future prospects of strategies for enhanced production efficiencies of PHB were also highlighted. Based on the overview of the current enhancing technologies, more pilot-scale and larger-scale tests are essential for future implementation of enhancing strategies in full-scale biogas plants. Critical analyses of various enhancing strategies would facilitate the establishment of more sustainable microbial fermentation systems for better waste management and greater efficiency of PHB production.

Bioaugmentation of Methanosarcina thermophila grown on biochar particles during semi-continuous thermophilic food waste anaerobic digestion under two different bioaugmentation regimes.

This study presents the effect of bioaugmentation of thermophilic anaerobic digestion of food waste with Methanosarcina thermophila grown on a wood-derived biochar. Two different supplementation regimes were tested, namely a single bioaugmentation (SBABC) in which 10% v/v of the microbes grown on biochar (1 g/L) is added at setup of the reactors, versus a routine bioaugmentation (RBABC) wherein the same amount of supplements were added over 10 feeding cycles. The optimally performing 'R' and 'S' reactors had increased methane yields by 37% and 32% over their respective controls while reactors SBABC 2 and 3 produced 21.89% and 56.09% higher average methane yield than RBABC 2 and 3, respectively. It appears that a single dose bioaugmentation is advantageous for improving AD as analysed in terms of average methane yield and VFA production. This study provides the basis for understanding how biochar and bioaugmentation can be used for engineering sustainable pilot-scale AD processes.

Engineered microbes as effective tools for the remediation of polyaromatic aromatic hydrocarbons and heavy metals.

Heavy metals (HMs) and polycyclic aromatic hydrocarbons (PAHs) have become a major concern to human health and the environment due to rapid industrialization and urbanization. Traditional treatment measures for removing toxic substances from the environment have largely failed, and thus development and advancement in newer remediation techniques are of utmost importance. Rising environmental pollution with HMs and PAHs prompted the research on microbes and the development of genetically engineered microbes (GEMs) for reducing pollution via the bioremediation process. The enzymes produced from a variety of microbes can effectively treat a range of pollutants, but evolutionary trends revealed that various emerging pollutants are resistant to microbial or enzymatic degradation. Naturally, existing microbes can be engineered using various techniques including, gene engineering, directed evolution, protein engineering, media engineering, strain engineering, cell wall modifications, rationale hybrid design, and encapsulation or immobilization process. The immobilization of microbes and enzymes using a variety of nanomaterials, membranes, and supports with high specificity toward the emerging pollutants is also an effective strategy to capture and treat the pollutants. The current review focuses on successful bioremediation techniques and approaches that make use of GEMs or engineered enzymes. Such engineered microbes are more potent than natural strains and have greater degradative capacities, as well as rapid adaptation to various pollutants as substrates or co-metabolizers. The future for the implementation of genetic engineering to produce such organisms for the benefit of the environment andpublic health is indeed long and valuable.

Methanosarcina thermophila bioaugmentation and its synergy with biochar growth support particles versus polypropylene microplastics in thermophilic food waste anaerobic digestion.

Both biochar supplementation as well as bioaugmentation have been shown in literature to improve the methane yield of anaerobic digestion. In this study, the combination of both are evaluated by growing Methanosarcina thermophila on biochar support particles prior to augmentation of thermophilic food waste anaerobic digestion. Biochar stand alone, bioaugmentation solely, a combination of both added separately or grown together, and utilizing polypropylene (PP) microplastics as growth support instead were all tested when starting up a thermophilic process from mesophilic inoculum. Methanosarcina thermophila and biochar supplementation displayed synergy, with 5% M. thermophila on 1 g/L biochar presenting a 32% increase in specific methane yield over the control. Double the bioaugmentation dosage/concentration was also trialled with a thermophilic inoculum, and 10% M. thermophila grown on 2 g/L biochar displayed the best results with a 20% increase specific methane yield from its control standard.

Microbial succession analysis reveals the significance of restoring functional microorganisms during rescue of failed anaerobic digesters by bioaugmentation of nano-biochar-amended digestate.

Nano-biochar application was investigated for anaerobic digestion of orange peel waste. The application for methane production focused on the optimization of biochar feedstock, rescue of failed digesters, and microbial succession analysis. It showed that sewage sludge (SS) derived biochar had the highest performance enhancement among the different feedstocks, which could be ascribed to the improvement of electron transfer, interspecies hydrogen transfer, and supply of trace elements. Subsequently, nano SS biochar-amended digestate was evaluated for rescuing failed digesters, and the experimental results indicated its positive roles through gradual bioaugmentation operation. The dynamic analysis of microbial succession indicated the successful application was through the mechanism of restoring partially the functional microbial communities. The major reconstruction of functional microorganisms included bacteria phyla Hydrogenispora (24.5%) and Defluviitoga (18.8%) as well as methanogenic genera of Methanosarcina (41.5%) and Methanobacterium (27.3%). These findings would contribute to rescuing failed anaerobic digesters by bioaugmentation with biochar-amended digestate.