Structure-function analysis of plant G-protein regulatory mechanisms identifies key Gα-RGS protein interactions.

Heterotrimeric GTP-binding protein alpha subunit (Gα) and its cognate regulator of G-protein signaling (RGS) protein transduce signals in eukaryotes spanning protists, amoeba, animals, fungi, and plants. The core catalytic mechanisms of the GTPase activity of Gα and the interaction interface with RGS for the acceleration of GTP hydrolysis seem to be conserved across these groups; however, the RGS gene is under low selective pressure in plants, resulting in its frequent loss. Our current understanding of the structural basis of Gα:RGS regulation in plants has been shaped by Arabidopsis Gα, (AtGPA1), which has a cognate RGS protein. To gain a comprehensive understanding of this regulation beyond Arabidopsis, we obtained the x-ray crystal structures of Oryza sativa Gα, which has no RGS, and Selaginella moellendorffi (a lycophyte) Gα that has low sequence similarity with AtGPA1 but has an RGS. We show that the three-dimensional structure, protein-protein interaction with RGS, and the dynamic features of these Gα are similar to AtGPA1 and metazoan Gα. Molecular dynamic simulation of the Gα-RGS interaction identifies the contacts established by specific residues of the switch regions of GTP-bound Gα, crucial for this interaction, but finds no significant difference due to specific amino acid substitutions. Together, our data provide valuable insights into the regulatory mechanisms of plant G-proteins but do not support the hypothesis of adaptive co-evolution of Gα:RGS proteins in plants.

Overexpression of a Plant-Specific Gγ Protein, AGG3, in the Model Monocot Setaria viridis Confers Tolerance to Heat Stress.

The vascular plant-specific, cysteine-rich type III Gγ proteins, which are integral components of the heterotrimeric G-protein complex, play crucial roles in regulating a multitude of plant processes, including those related to crop yield and responses to abiotic stresses. The presence of multiple copies of type III Gγ proteins in most plants and a propensity of the presence of specific truncated alleles in many cultivated crops present an ambiguous picture of their roles in modulating specific responses. AGG3 is a canonical type III Gγ protein of Arabidopsis, and its overexpression in additional model crops offers the opportunity to directly evaluate the effects of protein expression levels on plant phenotypes. We have shown that AGG3 overexpression in the monocot model Setaria viridis leads to an increase in seed yield. In this study, we have investigated the response of the S. viridis plants overexpressing AGG3 to heat stress (HS), one of the most important abiotic stresses affecting crops worldwide. We show that a short span of HS at a crucial developmental time point has a significant effect on plant yield in the later stages. We also show that plants with higher levels of AGG3 are more tolerant to HS. This is attributed to an altered regulation of stress-responsive genes and improved modulation of the photosynthetic efficiency during the stress. Overall, our results confirm that AGG3 plays a crucial role in regulating plant responses to unfavorable environmental conditions and may contribute positively to avoiding crop yield losses.

Manejo de urgencias odontológicas en pacientes afiliados al Seguro Social Campesino El Porvenir, El Carmen, Ecuador

Introducción: las urgencias dentales que se presentan a diario en el campo odontológico son de suma importancia, de ahí que sea necesario saber manejarlas y así evitar consecuencias posteriores que pueden poner en riesgo la vida. Objetivo: diagnosticar cómo se manejan las urgencias odontológicas en el Instituto Ecuatoriano de Seguridad Social Campesino. Materiales y métodos: estudio descriptivo, cuantitativo y transversal, con una muestra de 60 usuarios. Se tomaron, además, criterios de inclusión y de exclusión. Se utilizaron como técnicas de recolección de datos encuestas estructuradas -basadas en los protocolos de odontología del Ministerio de Salud Pública (2014) y el protocolo de urgencias odontológica COVID (2020)-, las que indicaron el manejo de las urgencias en el área mencionada. Se evaluó el servicio mediante los porcentajes que se reflejaron en la encuesta sobre el manejo de urgencias y los tipos de patologías de mayor frecuencia. Resultados: el 82 % de los encuestados manifestó que recibió una atención inmediata a su urgencia dental. Un 95 % refirió que les dieron una atención adecuada según su urgencia de calidad. Además, se evaluaron los tipos de urgencias más frecuentes, destacando infección dental (50 %), caries profunda (20 %), extracción (15 %), fractura (8 %) y tercer molar impactado (2 %). Conclusiones: se concluye que en el Seguro Social Campesino El Porvenir destacó el buen manejo en las urgencias odontológicas. Esto se ve reflejado en la utilización de maniobras y protocolos que están determinados en el área de la odontología.

Introduction: dental urgencies that daily present in the odontological field are of great importance, so it is necessary to know how to manage them and that way to avoid posterior consequences that could put life at risk. Objective: to diagnose how to manage odontological urgencies in the Ecuadorian Institute of Rural Social Security Materials and methods: descriptive, quantitative, cross-sectional study, with a sample of 60 users. Inclusion and exclusion criteria were also taken in to account. Structured inquiries were used as data collection techniques-based on the odontological protocols of the Ministry of Public Health (2014) and the COVID odontological emergency protocol (2020)-, which indicate emergencies management in the mentioned field. The service was evaluated through the percentages reflected in the emergency management enquiry and the most frequent types of pathologies. Results: 82% of those surveyed stated that they received an immediate attention to their dental emergency. 95% said that they were given an adequate attention according to their quality emergency. Besides that, the most frequent emergency types were evaluated, highlighting dental infection (50%), deep caries (20%), extraction (15%), fracture (8%) and impacted third molar (2%). Conclusion: It is concluded that in the Rural Social Security El Porvenir, the good management of odontological urgencies stood out. This is reflected in the use of maneuvers and protocols that are determined in the area of dentistry.

[Distribution of triglycerides and glucose (TyG) index and homeostasis model assessment insulin resistance for the evaluation of insulin sensitivity on late adolescence in Mexicans]. / Distribución del índice triglicéridos y glucosa (TyG) y el modelo homeostático para la evaluación de la resistencia a la insulina en la adolescencia tardía en mexicanos.

Introduction: Introduction: when peripheral tissues don't respond well to insulin action, it is defined as insulin resistance (IR). Many methods and indices are available for the estimation of IR, among them the homeostasis model assessment of insulin resistance (HOMA-IR) involves fasting plasma glucose and insulin. Nevertheless, the TyG index has a methodological advantage over the HOMA-IR because it requires only measurements provided by routine laboratory tests. Aim: distribution asessment of the HOMA-IR and TyG indexes in the sample. Also, to determine the predictive capacity of HOMA-IR, using TyG cutoff point as IR-positive diagnostic test. Materials and methods: a cross-sectional analytical study with 1686 participants aged 18 to 21 years from the state of San Luis Potosí, Mexico. Anthropometric assessment involves variables of weight and height. Fasting glucose, insulin and triglyceride concentrations were quantified. In addition, a questionnaire was carried out to know the hereditary family history and the presence of noncommunicable diseases (NCDs). Student's t-test was used to assess the differences in mean statistics between males and females. A receiver operating characteristic (ROC) curve was applied to examine the potential of HOMA-IR to identify IR. Results: 56 % of the study adolescents were females and 44 % were males; mean BMI was 22.62 ± 3.21 kg/m2. In the total sample mean serum glucose, insulin, and triglyceride concentrations were 89.48 ± 9.84 mg/dL, 6.26 ± 5.04 µU/mL, and 95.64 ± 55.78 mg/dL, respectively. A prevalence of 28.2 % of IR was determined, evaluated with the cut-off points for the TyG index. Subsequently, Receiver Operator Curves (ROC) were performed to evaluate the predictive capacity of HOMA-IR. The most outstanding cut-off value was 1.08 for the HOMA-IR index, reaching a sensitivity of 66 % and a specificity of 53 %. The prevalence of HOMA-IR greater than or equal to 1.18 was 47 % in the total population, 19.3 % in males and 28.5 % in females Conclusions: HOMA-IR and TyG can be useful diagnostic parameters for the assessment of IR in late adolescence. To provide a health guide for IR, we propose that a HOMA-IR target value ≤ 1.08 should be considered.

Introducción: Introducción: cuando los tejidos periféricos tienen una incapacidad para responder a la acción de la insulina se denomina resistencia a la insulina (RI). Existen diferentes métodos para la identificación de la RI; uno de estos es el índice HOMA-IR, que utiliza los parámetros de laboratorio, glucosa e insulina en ayunas. El índice triglicéridos y glucosa (TyG) presenta la ventaja de solo necesitar análisis de laboratorio de rutina. Objetivo: evaluación de la distribución de los índices HOMA-IR y TyG en la población, así como determinar la capacidad predictiva del índice HOMA-IR utilizando el TyG como prueba diagnóstica para la RI. Materiales y métodos: estudio analítico transversal con 1686 participantes de 18 a 21 años del estado de San Luis Potosí. Se tomaron variables antropométricas de peso y talla y se cuantificó la concentración de glucosa, insulina y triglicéridos en ayuno. Además, se realizó un cuestionario para conocer los antecedentes heredofamiliares y la presencia de enfermedades no transmisibles (ENT). Para la comparación entre mujeres y hombres se realizó una prueba de la t de Student y se realizaron curvas operador receptor (COR) para determinar los valores de corte del HOMA-IR. Resultados: el 56 % de la población fueron mujeres y el 44 % hombres; la media del IMC fue de 22,62 ± 3,21 kg/m2. En la población total de estudio, la media de la concentración sérica de glucosa, insulina y triglicéridos fue de 89,48 ± 9,84 mg/dL, 6,26 ± 5,04 µU/mL y 95,64 ± 55,78 mg/dL, respectivamente. Se determinó una prevalencia del 28,2 % de la RI evaluada con los puntos de corte para el índice TyG. Posteriormente se realizaron curvas operador receptor (COR) para evaluar la capacidad predictiva del HOMA-IR. El valor de corte más destacado fue de 1,08 para el índice HOMA-IR, alcanzando una sensibilidad del 66 % y una especificidad del 53 %. La prevalencia del HOMA-IR mayor o igual a 1,18 fue del 47 % en la población total, del 19,3 % en los hombres y del 28,5 % en las mujeres. Conclusiones: los índices HOMA-IR y TyG pueden ser parámetros de utilidad diagnóstica para la valoración de la RI en la adolescencia tardía. Con el objetivo de brindar una guía de salud para la RI, proponemos que se debe considerar como objetivo un valor de HOMA-IR ≤ 1,08.

Distribución del índice triglicéridos y glucosa (TyG) y el modelo homeostático para la evaluación de la resistencia a la insulina en la adolescencia tardía en mexicanos / Distribution of triglycerides and glucose (TyG) index and homeostasis model assessment insulin resistance for the evaluation of insulin sensitivity on late adolescence in Mexicans

Introducción: cuando los tejidos periféricos tienen una incapacidad para responder a la acción de la insulina se denomina resistencia a la insulina (RI). Existen diferentes métodos para la identificación de la RI; uno de estos es el índice HOMA-IR, que utiliza los parámetros de laboratorio, glucosa e insulina en ayunas. El índice triglicéridos y glucosa (TyG) presenta la ventaja de solo necesitar análisis de laboratorio de rutina. Objetivo: evaluación de la distribución de los índices HOMA-IR y TyG en la población, así como determinar la capacidad predictiva del índice HOMA-IR utilizando el TyG como prueba diagnóstica para la RI. Materiales y métodos: estudio analítico transversal con 1686 participantes de 18 a 21 años del estado de San Luis Potosí. Se tomaron variables antropométricas de peso y talla y se cuantificó la concentración de glucosa, insulina y triglicéridos en ayuno. Además, se realizó un cuestionario para conocer los antecedentes heredofamiliares y la presencia de enfermedades no transmisibles (ENT). Para la comparación entre mujeres y hombres se realizó una prueba de la t de Student y se realizaron curvas operador receptor (COR) para determinar los valores de corte del HOMA-IR. (AU)

Introduction: when peripheral tissues don't respond well to insulin action, it is defined as insulin resistance (IR). Many methods and indices are available for the estimation of IR, among them the homeostasis model assessment of insulin resistance (HOMA-IR) involves fasting plasma glucose and insulin. Nevertheless, the TyG index has a methodological advantage over the HOMA-IR because it requires only measurements provided by routine laboratory tests. Aim: distribution asessment of the HOMA-IR and TyG indexes in the sample. Also, to determine the predictive capacity of HOMA-IR, using TyG cutoff point as IR-positive diagnostic test. Materials and methods: a cross-sectional analytical study with 1686 participants aged 18 to 21 years from the state of San Luis Potosí, Mexico. Anthropometric assessment involves variables of weight and height. Fasting glucose, insulin and triglyceride concentrations were quantified. In addition, a questionnaire was carried out to know the hereditary family history and the presence of noncommunicable diseases (NCDs). Student's t-test was used to assess the differences in mean statistics between males and females. A receiver operating characteristic (ROC) curve was applied to examine the potential of HOMA-IR to identify IR. (AU)

Test predictores de vía aérea difícil y hallazgos bajo laringoscopia directa en sala de operaciones del hospital general esmeraldas sur delfina torres de concha / Difficult airway predictor tests and findings under direct laryngoscopy in the operating room of Esmeraldas Sur General Hospital Delfina Torres de Concha

La combinación de los test predictores de la vía área difícil durante la evaluación preanestésica y la preparación de los pacientes quirúrgicos es fundamental para reducir el índice de morbimortalidad. Objetivo: Analizar la relación entre los test predictores de vía aérea difícil y los hallazgos bajo laringoscopia directa en los pacientes que son intervenidos en la sala de operaciones del Hospital General Esmeraldas Sur Delfina Torres de Concha. La institución de salud en mención no registra previamente un estudio de estas características. Materiales y Métodos: El diseño de investigación que se aplicó fue cualitativo, de corte transversal con enfoque descriptivo. En consecuencia, se observaron y se tomaron datos del formulario de anestesiología de 150 historias clínicas de pacientes que fueron derivados a cirugía desde febrero de 2019 hasta julio de 2019. Las variables examinadas correspondieron a paciente adulto, vía aérea difícil, test predictores de VAD y laringoscopia directa. Resultados: Mostraron que el test que alertó más casos de VAD es el de protrusión mandibular con el 59,30%, seguido de la distancia tiromentoniana con el 40,00%. Asimismo, los hallazgos bajo laringoscopia derivaron en procedimientos de intubación difícil, guardando relación con otros test predictores de VAD. Conclusiones: La combinación de varios test de VAD facultan a los médicos a planificar respuestas oportunas ante la presencia de problemas(AU)

The combination of predictive tests of the difficult airway during the pre-anesthetic evaluation and the preparation of surgical patients is essential to reduce the morbidity and mortality rate. Objective: To analyze the relationship between the difficult airway predictive tests and the findings under direct laryngoscopy in patients who are operated on in the operating room of the Hospital General Esmeraldas Sur Delfina Torres de Concha. The aforementioned health institution has not previously registered a study of these characteristics. Materials and Methods: The research design that was applied was qualitative, cross-sectional with a descriptive approach. Consequently, data from the anesthesiology form of 150 medical records of patients who were referred for surgery from February 2019 to July 2019 were observed and collected. The variables examined corresponded to adult patients, difficult airway, VAD predictor tests and direct laryngoscopy. Results: They showed that the test that alerted more cases of VAD is mandibular protrusion with 59.30%, followed by thyromental distance with 40.00%. Likewise, the findings under laryngoscopy led to difficult intubation procedures, being related to other VAD predictive tests. Conclusions: The combination of several VAD tests empower physicians to plan timely responses to the presence of problems(AU)

High resolution crystal structure of NaTrxh from Nicotiana alata and its interaction with the S-RNase.

Thioredoxins are regulatory proteins that reduce disulfide bonds on target proteins. NaTrxh, which belongs to the plant thioredoxin family h subgroup 2, interacts and reduces the S-RNase enhancing its ribonuclease activity seven-fold, resulting an essential protein for pollen rejection inNicotiana.Here, the crystal structure of NaTrxh at 1.7 Å by X-ray diffraction is reported. NaTrxh conserves the typical fold observed in other thioredoxins from prokaryotes and eukaryotes, but it contains extensions towards both N- and C-termini.The NaTrxh N-terminal extension participates in the reduction of S-RNase, and in the structure reported here, this is orientated towards the reactive site. The interaction between SF11-RNase and the NaTrxh N-terminal was simulated and the short-lived complex observed lasted for a tenth of ns. Moreover, we identified certain amino acids as SF11-RNase-E155 and NaTrxh-M104 as good candidates to contribute to the stability of the complex. Furthermore, we simulated the reduction of the C153-C186 SF11-RNase disulfide bond and observed subtle changes that affect the entire core, which might explain the increase in the ribonuclease activity of S-RNase when it is reduced by NaTrxh.

NaTrxh is an essential protein for pollen rejection in Nicotiana by increasing S-RNase activity.

In self-incompatible Solanaceae, the pistil protein S-RNase contributes to S-specific pollen rejection in conspecific crosses, as well as to rejecting pollen from foreign species or whole clades. However, S-RNase alone is not sufficient for either type of pollen rejection. We describe a thioredoxin (Trx) type h from Nicotiana alata, NaTrxh, which interacts with and reduces S-RNase in vitro. Here, we show that expressing a redox-inactive mutant, NaTrxhSS , suppresses both S-specific pollen rejection and rejection of pollen from Nicotiana plumbaginifolia. Biochemical experiments provide evidence that NaTrxh specifically reduces the Cys155 -Cys185 disulphide bond of SC10 -Rnase, resulting in a significant increase of its ribonuclease activity. This reduction and increase in S-RNase activity by NaTrxh helps to explain why S-RNase alone could be insufficient for pollen rejection.

Variability in the Phytochemical Contents and Free Radical-Scavenging Capacity of Capsicum annuum var. glabriusculum (Wild Piquin Chili).

The variability in the phytochemical concentrations of Capsicum annuum var. glabriusculum has not been extensively analyzed among wild populations and ecologic niches in its phylogeographic area. This study aimed to determine the variations in the phytochemical and antioxidant contents of the wild Piquin chili. The total flavonoid content of hydroalcoholic extracts (0.06 to 0.70 mg equivalent of quercetin per gram of dry weight (mg QE/g DW)), free radical-scavenging capacity for 2,2-diphenyl-1-picrylhydrazyl (DPPH. ) radicals (0.55 to 8.55 mm TE/g DW), amount of 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+ ) in aqueous extracts (18.13 to 107.6 mm TE/g DW) and pungency (21,760 to 88,476 Scoville heat units) were highly variable. By analyzing the spatial distribution using the first three principal components, correlations between the phytochemical content and the free radical-scavenging capacity (in both extracts) and flavonoid and phenolic contents (in the hydroalcoholic extract) were observed. Consistent with the statistical analysis, the spatial analysis showed intraregional differences in composition patterns, with an emphasis on central and coastal areas. Flavonoid contents, polyphenol contents and free radical-scavenging activity were the phytochemical components that mainly contributed to the diversity of the population.

Aceptación de la Medicina Tradicional en Codesa y Tabiazo del Cantón Esmeraldas / Acceptance of Traditional Medicine in Codesa and Tabiazo of Esmeraldas Canton

Fundamentación: La medicina tradicional es de uso frecuente en comunidades afrodescendientes e indígenas, pero si no se disponen de amplios conocimientos sobre la misma, pueden generar efectos nocivos en la salud. Objetivo: Determinar la aceptación de la población sobre la medicina tradicional en el sector Codesa y la parroquia Tabiazo, del cantón Esmeraldas. Método: Se realizó un estudio mixto (CUAN/cual), con alcance descriptivo, donde se detalló el nivel de aceptación de la medicina tradicional en dos grupos de estudio, para valorar el conocimiento, uso y satisfacción de esta. Se empleó un cuestionario auto- desarrollado en el que se identificó la población que aplica la medicina tradicional, sus factores socioculturales y las limitantes económicas que los lleva a su uso. En el estudio se incluyeron dos grupos poblacionales que fueron seleccionados a través de muestreo tipo probabilístico por racimos, del área urbana y rural, con 150 participantes respectivamente de cada área. Resultados: El 92% del área urbana posee conocimientos sobre la medicina tradicional, frente al 98% del sector rural. El 90% del área urbana acepta la medicina tradicional, mientras el área rural lo hace en el 96%. Sobre la práctica de la medicina alternativa, el 84% del sector urbano y el 91% del rural presentan prácticas relacionadas con la medicina ancestral. Entre los principales factores que inducen en estas prácticas se encuentran el aspecto cultural, con el 30%, el 27% por bajo costo y el 43% por nivel de efectividad y pocos efectos secundarios. Conclusión: Ambos sectores aceptan y aplican la medicina tradicional. En el área rural se da en mayor frecuencia, por factores influyentes como el conocimiento, la cultura y el nivel de accesibilidad.

Background: Traditional medicine is frequently used in Afro-descendant and indigenous communities, but if there is not extensive knowledge about it, it can generate harmful effects on health. Objective: To determine the acceptance of the population on traditional medicine in the Codesa sector and the Tabiazo parish, in the Esmeraldas canton. Method: A mixed study was carried out (QUAN / qual), with a descriptive scope, where the level of acceptance of traditional medicine in two study groups was detailed, to assess the knowledge, use and satisfaction with it. A self-developed questionnaire was used to identify the population that applies traditional medicine, their socio-cultural factors and the economic limitations that lead to its use. The study included two population groups that were selected through probabilistic sampling by cluster, from the urban and rural areas, with 150 participants, respectively, from each area. Results: 92% of the urban area has knowledge about traditional medicine, compared to 98% of the rural sector. 90% of the urban area accepts traditional medicine, while the rural area does so at 96%. On the practice of alternative medicine, 84% of the urban sector and 91% of the rural sector present practices related to ancestral medicine. Among the main factors that induce these practices are the cultural aspect, with 30%; 27% for its low cost and 43% for the level of effectiveness and few side effects. Conclusion: Both sectors accept and apply traditional medicine. In the rural area it occurs more frequently, due to influential factors such as knowledge, culture and level of accessibility.

Detection of Zika virus in Aedes mosquitoes from Mexico.

Background: We report on the results of an entomovirological surveillance system of Aedes populations performed by the Ministry of Health of the central state of San Luis Potosí, Mexico. Methods: Indoor adult Aedes aegypti and Aedes albopictus pools collected at San Martín, Tamazunchale, Ciudad Valles, Metlapa, Ebano, Tamuin and Axtla during the dry season of 2016 were examined for the presence of dengue (DENV), chikungunya (CHIKV) and Zika (ZIKV) viruses using real-time PCR. Results: Both Ae. aegypti and Ae. albopictus were found to be infected with ZIKV in the absence of confirmed symptomatic human cases. Conclusions: The entomovirological surveillance system analysed here identified both Ae. aegypti and Ae. albopictus infected with ZIKV which triggered an immediate aggressive vector control campaign.

Complete Genome Sequence of Zika Virus Isolated in Mexico, 2016.

Zika virus belongs to the genus Flavivirus, and its spread remains an international public health emergency. In this report, we describe the obtainment and molecular characterization of a complete viral genome through the direct metagenomic analysis from saliva from an autochthonous transmission case in Mexico.

Anti-inflammatory and anti-oxidant effect of Calea urticifolia lyophilized aqueous extract on lipopolysaccharide-stimulated RAW 264.7 macrophages.

ETHNOPHARMACOLOGICAL RELEVANCE: Calea urticifolia leaves are traditionally used as a remedy to treat gastric ulcers, diabetes, and inflammation by the Xi'uy ancient native community of San Luis Potosi, Mexico. AIM OF THE STUDY: The aim was to assess the effects of the aqueous extract of the Mexican plant C. urticifolia as anti-inflammatory and anti-oxidant using lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and to provide evidence on the phenolic compounds. MATERIALS AND METHODS: RAW 264.7 macrophages were stimulated with 1µg/mL of LPS and treated with 10, 25 50, 75 y 100µg/mL of Calea urticifolia lyophilized aqueous extract (CuAqE). Nitric oxide (NO) release, tumor necrosis factor alpha, prostaglandin E2 production, inducible nitric oxide synthase (iNOS), cyclooxygenase-2, nuclear factor-κB (NF-κB) p65, NF-κB p50 expression and reactive oxygen species (ROS) were measured; other pro-inflammatory proteins were measured with membrane antibody array. Phenolic compounds were analyzed by LC-ESI-MS. RESULTS: Inflammation was inhibited by suppressing iNOS/NO pathway through inhibiting nucleus translocation of NF-κB p65 and p50 sub-units. ROS production was significantly (P<0.05) inhibited in a dose-dependent manner in LPS-stimulated macrophages. Moreover, the expression of inflammatory markers was suppressed (34.5-88.3%) by CuAqE. A mix of caffeoylquinic acid derivatives and flavonoid-glycosides were found in CuAqE. CONCLUSION: Phenolic compounds in CuAqE such as caffeoylquinic acid derivatives and flavonoid glycosides could be responsible for inhibiting LPS-induced inflammation and oxidative stress by iNOS/NO pathway through suppressing NF-κB signaling pathway and by inhibition of ROS production in RAW 264.7 macrophages. Therefore, these results support the traditional knowledge of C. urticifolia tea such as an anti-inflammatory and antioxidant agent.

Identification of Asian genotype of chikungunya virus isolated in Mexico.

We identified 25 autochthonous chikungunya virus cases in Mexico, initially detected by RT-PCR targeting the E1 gene and propagated in C6/36 Aedes albopictus cells, in 2014. To determine the type of virus found, in a previous report, the genomes of 2 CHIKV strains were fully sequenced. Genome sequence analysis revealed that these isolates from Mexico belonged to the Asian genotype, and a phylogenetic association with the circulating strain in the British Virgin Islands was also established in the same year. This was further supported by changes in specific amino acids, E2-V368A and 6K-L20M. For these reasons, it can be inferred that the route of virus entry to Mexico was held across the countries in the Caribbean and Central America. The presence of E1-A226V mutation associated with more efficient replication in the salivary gland of the A. albopictus mosquito was not observed. Interestingly, a newly acquired NSP4-S399C mutation was observed; however, the significance of changes in amino acid found in non-structural proteins in autochthonous strains remains to be elucidated.

Complete genome sequences of chikungunya virus strains isolated in Mexico: first detection of imported and autochthonous cases.

The mosquito-borne chikungunya virus, an alphavirus of the Togaviridae family, is responsible for acute polyarthralgia epidemics. Here, we report the complete genome sequences of two chikungunya virus strains, InDRE04 and InDRE51, identified in the Mexican states of Jalisco and Chiapas in 2014. Phylogenetic analysis showed that both strains belong to the Asian genotype.

Quiste perineural simulando masa anexial en estudio de paciente infértil / Perinerural cyst simulating an adnexal mass in the study of an infertile patient

Los quistes perineurales o de Tarlov son por lo general hallazgos incidentales durante un estudio de imagen que no requieren tratamiento a menos que produzcan síntomas. Tienen una incidencia baja en la población general y esto puede deberse a un error en su diagnóstico, ya que simulan otras lesiones quísticas de la pelvis.Presentamos el caso de una paciente de 38 años en estudio por infertilidad primaria que en control ecográfico, se aprecia lesión quística pélvica de 4 cm, de situación medial, y aparentemente independiente de anejos y útero. Tras dicho hallazgo, se vitrifican los embriones y se deriva al servicio de medicina interna y de neurocirugía para completar estudio.La TC abdomino-pélvica informa de la presencia formaciones quísticas que dependen de los agujeros de conjunción sacros y que se confirman en la RMN. Dichas formaciones son compatibles con el diagnóstico de quiste meníngeo extradural.Al no presentar clínica, se decide tratamiento conservador. La paciente regresa a la unidad de reproducción para completar tratamiento de infertilidad (AU)

Perineural or Tarlov cysts are usually detected as incidental findings during an imaging study and do not require treatment unless symptomatic. The incidence of perineural cysts in the general population is low. Because these cysts simulate other cystic lesions of the pelvis, their low incidence may be due to diagnostic error.We present the case of a 38-year-old woman who was under investigation for primary infertility. An ultrasound scan revealed a 4-cm pelvic cystic lesion, situated medially and apparently independently from the annexes and uterus. Subsequently, the embryos were vitrified and were sent to the internal medicine and neurosurgery services for examination.The abdominopelvic CT scan showed the presence of cystic formations in the intervertebral foramina of sacrum, which were confirmed by magnetic resonance imaging. These formations were compatible with a diagnosis of extradural meningeal cyst.Because the patient was asymptomatic, we decided to provide conservative treatment. The patient was referred back to the fertility unit to complete the fertility treatment (AU)

Biochemical characteristics and risk factors for insulin resistance at different levels of obesity.

OBJECTIVE: To establish the biochemical characteristics of nonobese, overweight, and obese children as well as to determine the risk factors associated with insulin resistance in nonobese children and with non-insulin resistance in obese children in the age strata of 6 to 11 years. METHODS: A total of 3512 healthy children were enrolled in a cross-sectional study. In the absence of obesity, fasting hyperinsulinemia and hypertriglyceridemia defined nonobese, insulin-resistant (NO-IR) children. In the absence of metabolic abnormalities of fasting insulin and triglycerides levels, obese children were defined as obese, not insulin-resistant (O-NIR) children. RESULTS: The gender- and age-adjusted prevalence of NO-IR and O-NIR was 6.6% and 21.3%, respectively. In the age-, gender-, and birth weight-adjusted analysis, family history of hypertension (FHH) in both maternal and paternal branches (odds ratio [OR]: 1.514; 95% confidence interval [CI]: 1.2-3.9; P = .04) was associated with NO-IR children. In the analysis adjusted by gender, age, waist circumference (WC), BMI, FHH, and family history of diabetes, high birth weight was associated with NO-IR children (OR: 1.319; 95% CI: 1.2-2.1; P = .04). Finally, in the gender-, age-, family history-, and birth weight-adjusted analysis, a WC lower than the 95th percentile was associated with a lower odds of insulin resistance among obese children (OR: 0.96; 95% CI: 0.91-0.98; P < .0005). CONCLUSIONS: FHH and high birth weight are associated with NO-IR children, and a low WC is associated with lower odds of O-IR children.

Atherogenic indices and prehypertension in obese and non-obese children.

We evaluate the relationship between different lipoproteins and atherogenic indices with pre-hypertension in 297 obese and 942 non-obese children with Tanner stage 1 enrolled in a multicentre, community-based cross-sectional study. Height, weight, fasting glucose and insulin levels, total cholesterol, triglycerides, high-density lipoprotein cholesterol (HDL-c), low-density lipoprotein cholesterol (LDL-c), non-HDL-c, LDL/HDL-c, triglycerides/cholesterol and total cholesterol/HDL-c ratios were measured. Mean age was 8.4 ± 1.2 years; pre-hypertension was identified in 104 (8.4%) participants, 46 (15.5%) obese and 58 (6.1%) non-obese children. The pre-hypertensive non-obese children show a high proportion of family history of hypertension (41.6 and 24.7%, p = 0.002) and elevation of insulin at a relatively low body mass index. The triglycerides:HDL-c ratio, but not other lipoproteins or atherogenic indices, was associated with pre-hypertension in obese (1.15, 95% confidence intervals 1.06-1.26) and non-obese children (1.38 95% confidence intervals 1.22-1.57). The triglycerides:HDL-c ratio is related to pre-hypertension in children; the family history of hypertension seems to be a risk factor in developing pre-hypertension.

Hidroadenoma papilífero vulvar. Presentación de un caso / Hidroadenoma papilliferum: a case report

El hidroadenoma papilífero (HP) es un tumor benigno de la vulva infrecuente que crece principalmente en la piel de la región anogenital de mujeres adultas. Puede plantear problemas de diagnóstico diferencial con los quistes y abscesos de la glándula de Bartolino, con el lipoma y con lesiones vulvares premalignas/y malignas. Se ha sugerido que pudiera derivar de glándulas análogas a las mamarias dispuestas a lo largo de la región vulvar y perineal femenina. Presentamos un caso de HP que se extirpó en una mujer de 32 años que tenía el antecedente de un parto por cesárea 5 meses atrás (AU)

Hidroadenoma papilliferum (HP) is a rare benign tumor of the vulva that grows mainly in the skin of the anogenital region of adult women. The differential diagnosis between this neoplasm and Bartholin cysts and abscesses, lipoma and some premalignant and malignant vulvar lesions can be difficult. HP may arise from mammary-like glands located in the vulvar and perineal region of women. We report a case of HP that was removed in a 32-year-old woman who had undergone a cesarean delivery 5 months previously (AU)