Asunto(s)
Osteomielitis , Humanos , Lactante , Recién Nacido , Osteomielitis/diagnóstico , Osteomielitis/terapiaRESUMEN
In the population of peripheral blood lymphocytes from systemic lupus erythematosus (SLE) patients activity of gamma-glutamyl transpeptidase (GGTP) was lower than in healthy individuals. However, when erythrocyte-rosette forming cells (E-RFC) and non erythrocyte-rosette forming cells (non E-RFC) were separated by E-RFC method it was found that in SLE patients the activity of GGTP was increased in E-RFC and decreased in the population of non E-RFC. The altered activity of GGTP may be due to impaired surface membranes and changes in activation of these cells in SLE patients.
Asunto(s)
Lupus Eritematoso Sistémico/enzimología , Linfocitos/enzimología , gamma-Glutamiltransferasa/sangre , Adulto , Femenino , Humanos , Lupus Eritematoso Sistémico/inmunología , Linfocitos/inmunología , Formación de RosetaRESUMEN
Non-esterified cholesterol was determined enzymatically with the cholesterol oxidase-peroxidase reaction using phenol and 4-aminophenazone as chromogens. This assay as done in the supernatant containing the lipoprotein-X. Other serum lipoproteins were removed by precipitation with heparin and zinc acetate followed by centrifugation. No standardization was required, the lipoprotein-X concentration being calculated with the use of the constant.
Asunto(s)
Colestasis/sangre , Lipoproteína X/sangre , Colesterol/sangre , Colesterol Oxidasa , Femenino , Peroxidasa de Rábano Silvestre , Humanos , MasculinoAsunto(s)
Factores de Edad , Aspirina/farmacología , Plaquetas/metabolismo , Indometacina/farmacología , Prostaglandina-Endoperóxido Sintasas/sangre , Prostaglandinas/biosíntesis , Vitamina E/farmacología , Adulto , Anciano , Plaquetas/enzimología , Femenino , Humanos , Peróxidos Lipídicos/sangre , Masculino , Persona de Mediana EdadAsunto(s)
Enfermedades del Desarrollo Óseo/complicaciones , Rótula/anomalías , Adolescente , Niño , Preescolar , Humanos , MasculinoRESUMEN
Turbidimetric determination of the cholestasis characterizing lipoprotein-X is based on the separation from the serum investigated of interfering lipoproteins by precipitation with the appropriate concentration of heparin and zinc acetate followed by centrifugation. In the supernatant the abnormal lipoprotein-X, which is not precipitated under these conditions, was determined by measuring the turbidity after addition of calcium chloride and heparin.
