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1.
J Hazard Mater ; 419: 126516, 2021 10 05.
Artículo en Inglés | MEDLINE | ID: mdl-34218189

RESUMEN

Microbial bioremediation has gained attention as a cheap, efficient, and sustainable technology to manage the increasing environmental pollution. Since microorganisms in nature are not evolved to degrade pollutants, there is an increasing demand for developing safer and more efficient pollutant-scavengers for enhanced bioremediation. In this review, we introduce the strategies and technologies developed in the field of synthetic biology and their applications to the construction of microbial scavengers with improved efficiency of biodegradation while minimizing the impact of genetically engineered microbial scavengers on ecosystems. In addition, we discuss recent achievements in the biodegradation of fastidious pollutants, greenhouse gases, and microplastics using engineered microbial scavengers. Using synthetic microbial scavengers and multidisciplinary technologies, toxic pollutants could be more easily eliminated, and the environment could be more efficiently recovered.


Asunto(s)
Ecosistema , Contaminantes Ambientales , Biodegradación Ambiental , Ingeniería Genética , Plásticos
2.
Commun Biol ; 4(1): 205, 2021 02 15.
Artículo en Inglés | MEDLINE | ID: mdl-33589718

RESUMEN

In bacterial biotechnology, instead of producing functional proteins from plasmids, it is often necessary to deliver functional proteins directly into live cells for genetic manipulation or physiological modification. We constructed a library of cell-penetrating peptides (CPPs) capable of delivering protein cargo into bacteria and developed an efficient delivery method for CPP-conjugated proteins. We screened the library for highly efficient CPPs with no significant cytotoxicity in Escherichia coli and developed a model for predicting the penetration efficiency of a query peptide, enabling the design of new and efficient CPPs. As a proof-of-concept, we used the CPPs for plasmid curing in E. coli and marker gene excision in Methylomonas sp. DH-1. In summary, we demonstrated the utility of CPPs in bacterial engineering. The use of CPPs would facilitate bacterial biotechnology such as genetic engineering, synthetic biology, metabolic engineering, and physiology studies.


Asunto(s)
Biotecnología , Péptidos de Penetración Celular/metabolismo , Proteínas de Escherichia coli/metabolismo , Escherichia coli/metabolismo , Microbiología Industrial , Methylomonas/metabolismo , Animales , Células CHO , Péptidos de Penetración Celular/genética , Cricetulus , Electroporación , Escherichia coli/genética , Proteínas de Escherichia coli/genética , Regulación Bacteriana de la Expresión Génica , Ingeniería Genética , Células HEK293 , Humanos , Methylomonas/genética , Biblioteca de Péptidos , Plásmidos/genética , Plásmidos/metabolismo , Prueba de Estudio Conceptual , Transporte de Proteínas
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