Microbial Diversity Analysis Using 16S rRNA Gene Amplicon Sequencing of Rhizosphere Soils from Double-Cropping Rice and Rice-Shrimp Farming Systems in Soc Trang, Vietnam.

Different rice farming systems affect the soil microbial communities. Here, we report the results of 16S rRNA gene amplicon sequencing of soils collected from intensive rice cultivation and rice-shrimp farming systems in Soc Trang, Vietnam. The dominant phyla in these systems were Firmicutes, Actinobacteriota, Chloroflexi, Myxococcota, and Acidobacteriota.

Rice husk and melaleuca biochar additions reduce soil CH 4 and N 2O emissions and increase soil physicochemical properties.

Background: Biochar is a promising material in mitigating greenhouse gases (GHGs) emissions from paddy fields due to its remarkable structural properties. Rice husk biochar (RhB) and melaleuca biochar (MB) are amendment materials that could be used to potentially reduce emissions in the Vietnamese Mekong Delta (VMD). However, their effects on CH 4 and N 2O emissions and soil under local water management and conventional rice cultivation have not been thoroughly investigated. Methods: We conducted a field experiment using biochar additions to the topsoil layer (0-20 cm). Five treatments comprising 0 t ha -1 (CT0); 5 t ha -1 (RhB5) and 10 t ha -1 (RhB10), and 5 t ha -1 (MB5) and 10 t ha -1 (MB10) were designed plot-by-plot (20 m 2) in triplicates. Results: The results showed that biochar application from 5 to 10 t ha -1 significantly decreased cumulative CH 4 (24.2 - 28.0%, RhB; 22.0 - 14.1%, MB) and N 2O (25.6 - 41.0%, RhB; 38.4 - 56.4%, MB) fluxes without a reduction in grain yield. Increasing the biochar application rate further did not decrease significantly total CH 4 and N 2O fluxes but was seen to significantly reduce the global warming potential (GWP) and yield-scale GWP in the RhB treatments. Biochar application improved soil Eh but had no effects on soil pH. Whereas CH 4 flux correlated negatively with soil Eh ( P < 0.001; r 2 = 0.552, RhB; P < 0.001; r 2 = 0.502, MB). The soil physicochemical properties of bulk density, porosity, organic matter, and anaerobically mineralized N were significantly improved in biochar-amended treatments, while available P also slightly increased. Conclusions: Biochar supplementation significantly reduced CH 4 and N 2O fluxes and improved soil mineralization and physiochemical properties toward beneficial for rice plant. The results suggest that the optimal combination of biochar-application rates and effective water-irrigation techniques for soil types in the MD should be further studied in future works.