RESUMEN
A serosurvey of antibodies against selected flaviviruses and alphaviruses in 384 bats (representing 10 genera and 14 species) was conducted in the Caribbean island of Trinidad. Sera were analysed using epitope-blocking enzyme-linked immunosorbent assays (ELISAs) specific for antibodies against West Nile virus (WNV), Venezuelan equine encephalitis virus (VEEV) and eastern equine encephalitis virus (EEEV), all of which are zoonotic viruses of public health significance in the region. Overall, the ELISAs resulted in the detection of VEEV-specific antibodies in 11 (2.9%) of 384 bats. Antibodies to WNV and EEEV were not detected in any sera. Of the 384 sera, 308 were also screened using hemagglutination inhibition assay (HIA) for antibodies to the aforementioned viruses as well as St. Louis encephalitis virus (SLEV; which also causes epidemic disease in humans), Rio Bravo virus (RBV), Tamana bat virus (TABV) and western equine encephalitis virus (WEEV). Using this approach, antibodies to TABV and RBV were detected in 47 (15.3%) and 3 (1.0%) bats, respectively. HIA results also suggest the presence of antibodies to an undetermined flavivirus(es) in 8 (2.6%) bats. Seropositivity for TABV was significantly (P<0.05; χ2) associated with bat species, location and feeding preference, and for VEEV with roost type and location. Differences in prevalence rates between urban and rural locations were statistically significant (P<0.05; χ2) for TABV only. None of the aforementioned factors was significantly associated with RBV seropositivity rates.
Asunto(s)
Infecciones por Alphavirus/epidemiología , Alphavirus/inmunología , Infecciones por Flavivirus/epidemiología , Flavivirus/inmunología , Infecciones por Alphavirus/sangre , Animales , Anticuerpos Antivirales/sangre , Quirópteros/virología , Virus de la Encefalitis Equina del Este , Virus de la Encefalitis Equina Venezolana , Ensayo de Inmunoadsorción Enzimática , Femenino , Infecciones por Flavivirus/sangre , Humanos , Masculino , Estudios Seroepidemiológicos , Trinidad y Tobago/epidemiología , Fiebre del Nilo OccidentalRESUMEN
Itacaiunas and Curionopolis viruses were isolated from Culicoides midges in Parauapebas municipality, Pará state, Brazil, in 1984 and 1985, respectively. Itacaiunas virus infected newborn mice and mosquito cells (C6/36), but did not replicate in some mammalian cell lineages; while Curionopolis virus infected only mice. Neither virus showed a serological relationship with any of the 195 known arboviruses circulating in Brazil, nor against 38 other rhabdoviruses isolated worldwide. Both virus particles are bullet-shaped and similar in morphology to that observed for other members of the family Rhabdoviridae. Partial nucleotide sequencing of the N protein showed that those two viruses constitute a separate clade in the family Rhabdoviridae, which we propose to be a new genus, designated Bracorhabdovirus.
Asunto(s)
Ceratopogonidae/virología , Rhabdoviridae/clasificación , Animales , Animales Recién Nacidos , Encéfalo/ultraestructura , Encéfalo/virología , Brasil , Ratones , Microscopía Electrónica , Rhabdoviridae/aislamiento & purificación , Rhabdoviridae/ultraestructuraRESUMEN
In 1998, an epizootic of yellow fever (YF) killed many howler monkeys (Alouatta spp.) in eastern Amazonia near the city of Altamira. An infection level with YF virus of approximately 3.6% was determined from analysis of 456 females of Haemagogus janthinomys Dyar, the main enzootic YF vector in South America. One month later, a second study of 164 females captured in the same place led to infection levels of 0.8% for parous and 2.9% for nulliparous females. These results lead to the conclusion that vertical transmission, one of the key elements in the epidemiology of YF, occurs in South America as it does in Africa.
Asunto(s)
Alouatta , Culicidae/virología , Insectos Vectores/virología , Enfermedades de los Monos/transmisión , Fiebre Amarilla/veterinaria , Virus de la Fiebre Amarilla/aislamiento & purificación , Animales , Brasil , Reservorios de Enfermedades/veterinaria , Femenino , Humanos , Transmisión Vertical de Enfermedad Infecciosa , Masculino , Ratones , Paridad , Fiebre Amarilla/transmisiónRESUMEN
This report describes a new hamster model for West Nile (WN) virus encephalitis. Following intraperitoneal inoculation of a New York isolate of WN virus, hamsters had moderate viremia of 5 to 6 days in duration, followed by the development of humoral antibodies. Encephalitic symptoms began 6 days after infection; about half the animals died between the seventh and 14th days. The appearance of viral antigen in the brain and neuronal degeneration also began on the sixth day. WN virus was cultured from the brains of convalescent hamsters up to 53 days after initial infection, suggesting that persistent virus infection occurs. Hamsters offer an inexpensive model for studying the pathogenesis and treatment of WN virus encephalitis.
Asunto(s)
Modelos Animales de Enfermedad , Mesocricetus , Fiebre del Nilo Occidental/virología , Virus del Nilo Occidental/fisiología , Animales , Anticuerpos Antivirales/inmunología , Antígenos Virales/análisis , Cricetinae , Femenino , Humanos , Técnicas para Inmunoenzimas , Etiquetado Corte-Fin in Situ , Viremia , Fiebre del Nilo Occidental/sangre , Fiebre del Nilo Occidental/inmunología , Fiebre del Nilo Occidental/patología , Virus del Nilo Occidental/genética , Virus del Nilo Occidental/crecimiento & desarrollo , Virus del Nilo Occidental/inmunologíaRESUMEN
A total of 187 different species of arboviruses and other viruses in vertebrates were identified at the Evandro Chagas Institute (IEC) from 1954 to 1998, among more than 10,000 arbovirus strains isolated from humans, hematophagous insects, and wild and sentinel vertebrates. Despite intensive studies in the Brazilian Amazon region, especially in Pará State, very little is known about most of these viruses, except for information on date, time, source, and method of isolation, as well as their capacity to infect laboratory animals. This paper reviews ecological and epidemiological data and analyzes the impact of vector and host population changes on various viruses as a result of profound changes in the natural environment. Deforestation, mining, dam and highway construction, human colonization, and urbanization were the main manmade environmental changes associated with the emergence and/or reemergence of relevant arboviruses, including some known pathogens for humans.
Asunto(s)
Infecciones por Arbovirus/epidemiología , Arbovirus/aislamiento & purificación , Vectores de Enfermedades , Ecosistema , Monitoreo del Ambiente/normas , Animales , Infecciones por Arbovirus/virología , Brasil/epidemiología , Conservación de los Recursos Naturales , Ecología , Monitoreo Epidemiológico , Humanos , UrbanizaciónRESUMEN
Oropouche (ORO) virus is an emerging infectious agent that has caused numerous outbreaks of an acute febrile (dengue-like) illness among humans in Brazil, Peru, and Panama. Diagnosis of ORO virus infection is based mainly on serology. Two different antigens, hamster serum antigen (HSA) and Vero cell lysate antigen (VCLA), are currently used in enzyme immunoassays (EIAs) in Brazil and Peru, respectively, to investigate the epidemiology of ORO virus infection. Both antigens involve use of infectious virus, and for this reason their use is restricted. Consequently, the frequency and distribution of ORO virus infection are largely unexplored in other countries of South America. This report describes the use of a bacterially expressed recombinant nucleocapsid (rN) protein of ORO virus in EIAs for the diagnosis of ORO virus infection. The data revealed that the purified rN protein is comparable to the authentic viral N protein in its antigenic characteristics and is highly sensitive and specific in EIAs. Among 183 serum samples tested, a high degree of concordance was found between rN protein-based EIA and HSA- and VCLA-based EIAs for the detection of both ORO virus-specific immunoglobulin M (IgM) and IgG antibodies. The high sensitivity, specificity, and safety of the rN protein-based EIA make it a useful diagnostic technique that can be widely used to detect ORO virus infection in South America.
Asunto(s)
Anticuerpos Antivirales/sangre , Infecciones por Bunyaviridae/diagnóstico , Técnicas para Inmunoenzimas , Proteínas de la Nucleocápside/inmunología , Virus Simbu/inmunología , Animales , Antígenos Virales/inmunología , Infecciones por Bunyaviridae/virología , Chlorocebus aethiops , Cricetinae , Humanos , Inmunoglobulina G/sangre , Inmunoglobulina M/sangre , Mesocricetus , Proteínas de la Nucleocápside/genética , Proteínas Recombinantes/inmunología , Células VeroRESUMEN
This report describes Trocara virus, a newly recognized member of the genus Alphavirus, that has been isolated from Aedes serratus mosquitoes collected at two widely separated sites in the Amazon Basin. Biological, antigenic and genetic characteristics of the new virus are given. Results of these studies indicate that Trocara virus is the first member of a newly discovered antigenic complex within the family Togaviridae genus Alphavirus. The public health and veterinary importance of Trocara virus is still unknown.
Asunto(s)
Aedes/virología , Alphavirus/genética , Alphavirus/aislamiento & purificación , Alphavirus/ultraestructura , Animales , Brasil , Pruebas de Fijación del Complemento , Cricetinae , Cartilla de ADN , Pruebas de Hemaglutinación , Ratones , Microscopía Electrónica , Perú , Reacción en Cadena de la Polimerasa , ARN Viral/aislamiento & purificación , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
The authors report the clinical, laboratorial and epidemiological aspects of a human case of jungle yellow fever. The patient suffered from fever, chills, sweating, headaches, backaches, myalgia, epigastric pains, nausea, vomiting, diarrhea and prostration. He was unvaccinated and had been working in areas where cases of jungle yellow fever had been confirmed. Investigations concerning the yellow fever virus were performed. Blood samples were collected on several days in the course of the illness. Three of these samples (those obtained on days 5, 7 and 10) were inoculated into suckling mice in attempt to isolate virus and to titrate the viremia level. Serological surveys were carried out by using the IgM Antibodies Capture Enzyme Linked Immunosorbent Assay (MAC-ELISA), Complement Fixation (CF), Hemagglutination Inhibition (HI) and Neutralization (N) tests. The yellow fever virus, recovered from the two first samples and the virus titration, showed high level of viremia. After that, specific antibodies appeared in all samples. The interval between the end of the viremia and the appearance of the antibodies was associated with the worsening of clinical symptoms, including bleeding of the mucous membrane. One must be aware of the risk of having a urban epidemics in areas where Aedes aegypti is found in high infestation indexes.
Asunto(s)
Fiebre Amarilla/virología , Adulto , Aedes/virología , Animales , Brasil , Humanos , Masculino , Ratones , Pruebas Serológicas , Viremia/diagnóstico , Viremia/virología , Fiebre Amarilla/diagnóstico , Fiebre Amarilla/inmunología , Fiebre Amarilla/terapia , Virus de la Fiebre Amarilla/aislamiento & purificaciónRESUMEN
Descrevem-se os resultados de inquérito sorológico para pesquisa de anticorpos de Hantavírus conduzido de fevereiro de 1986 a agosto de 1990, em grupos populacionais humanos dos Estados de Säo Paulo e Paraná, que apresentavam estreito contato com ratos urbanos ou com roedores e outros pequenos mamíferos silvestres. OBJETIVO. Identificar infecçäo humana causada por Hantavírus, agentes etiológicos da febre hemorrágica com síndrome renal. MÉTODOS. Os soros de 1.063 pessoas foram examinados com técnica imunoenzimática para detecçäo de anticorpos IgG e IgM utilizando-se antígenos dos vírus Hantaan, Seoul e Puumala. Nos soros positivos realizou-se teste de neutralizaçäo com reduçäo em placa para os mesmos antígenos e imunofluorescência indireta para o antígeno do vírus Hantaan. RESULTADOS. Observou-se presença de anticorpos anti-Hantavirus em 32 pessoas, evidenciados por testes imunoenzimáticos e de imunofluorescência. Em cinco delas foram positivos os testes de neutralizaçäo com reduçäo em placa (três para o vírus Scoul e dois para o vírus Pumala). Uma dessas pessoas apresentou alto título em todos os testes para o vírus Seoul. Trata-se de um morador da zona rural da regiäo do vale do Ribeira, que nasceu, sempre viveu no local e nunca viajou para fora do Estado de Säo Paulo. CONCLUSOES. Há evidências sorológicas de infecçäo causada por Hantavírus em moradores de área rural da Ilha do Amparo, Baia de Paranaguá, e da regiäo do Vale do Ribeira, Estado de Säo Paulo, em portuários de Paranaguá, Estado do Paraná, e em doentes internados com suspeita de leptospirose no Hospital Emílio Ribas, Säo Paulo
Asunto(s)
Ratas , Humanos , Animales , Infecciones por Hantavirus/diagnóstico , Brasil , Ensayo de Inmunoadsorción Enzimática , Técnica del Anticuerpo Fluorescente , Pruebas de NeutralizaciónRESUMEN
Here in is described the clinical and laboratorial findings of a laboratory-acquired infection caused by the virus SP H 114202 (Arenavirus, family Arenaviridae) a recently discovered agent responsible for a viral hemorrhagic fever. The patient was sick for 13 days. The disease had an abrupt onset characterized by high fever (39 degree C.), headache, chills and myalgias for 8 days. In addition, on the 3rd day, the patient developed nausea and vomiting, and in the 10th, epigastralgia, diarrhea and gengivorrhagia. Leucopenia was seen within the 1st week of onset, with counts as low as 2,500 white cells per mm3. Counts performed after the 23rd day of the onset were within normal limits. With the exception of moderate lymphocytosis, no changes were observed in differential counts. An increase in the titer of antibodies by complement fixation, neutralization and ELISA (IgM) was detected. Suckling mice and baby hamsters were inoculated intracerebrally with 0.02 ml of blood samples collected in the 2nd and 7th days of disease. Attempts to isolate the virus were also made in Vero cells. No virus was isolated. This virus was isolated before in a single occasion in São Paulo State, in 1990, from the blood of a patient with hemorrhagic fever with a fatal outcome. The manipulation of the virus under study, must be done carefully, since the transmission can occur through aerosols.
Asunto(s)
Infecciones por Arenaviridae/virología , Arenaviridae/aislamiento & purificación , Infección de Laboratorio/virología , Adulto , Animales , Infecciones por Arenaviridae/diagnóstico , Humanos , Infección de Laboratorio/diagnóstico , Masculino , RatonesRESUMEN
We report the first outbreak of dengue fever caused by dengue 2 (DEN 2) in Araguaina, Tocantins State. Four hundred people of 74 families, living at S. João, Araguaina Sul and Neblina districts were questioned and then bled, in order to obtain sera to test for anti-dengue antibodies. If a person was sick, a small quantity of blood was collected for virus isolation. The main clinical picture of disease was characterized by fever, headache, myalgias, arthralgias and skin rash. Were obtained 1,105 (56 females and 45 males of Culex quinquefasciatus and 567 females and 437 males of Aedes aegypti) mosquitoes from larvae collected in Araguaina. The females of Aedes aegypti obtained from larvae were allowed to feed on 8 febrile patients. The diagnosis of infection was made by both virus isolation into Aedes albopictus (C6/36) cells, and serology, by Hemagglutination-inhibition (HI) and IgM capture ELISA (MAC ELISA). No virus was isolated from mosquitoes. Although five strains of DEN 2 were obtained from humans, and another 111 infections were diagnosed serologically (IgM positive). The positivity rate of the samples was 27.75% (111 of 400), while that of the families was 66.2% (45 of 72), where at least one member of the each family was infected. It was also detected 26.1% of asymptomatic infections. All age groups were affected. Therefore, the infection was more frequent in females (33.5%) than males (23.8%).(ABSTRACT TRUNCATED AT 250 WORDS)
Asunto(s)
Dengue/epidemiología , Adolescente , Adulto , Distribución por Edad , Brasil/epidemiología , Niño , Preescolar , Virus del Dengue/aislamiento & purificación , Femenino , Humanos , Lactante , Recién Nacido , Masculino , Persona de Mediana Edad , Distribución por SexoRESUMEN
The conditions of maintenance of YF virus in brazilian Amazonia are not yet elucidated. Generally, the presence of the virus is attested by human cases of sylvatic origin. During a survey done at the exact place where a man have probably been contaminated, it was possible for the first time in South America, to estimate the mean parity rate of a population of the potential vector Haemagogus janthinomys, from which the YF virus was actually isolated. The survival rate (Ts = 0.96), the biting rate (0.60 mosquitoes/man x hour), and the infection rate (1.71%) were also determinated for the same mosquitoes and have values compatible with the probable conditions of the human contamination. However, more data are needed, in particular in relation with other possible human contaminations and/or circulation of the YF virus in the monkey population (extension and duration of the epizootic episode), in order to know what maintenance cycle is prevalent in this region: a low level transmission, with the mosquito being a "vector-reservoir", or a "constantly moving epizootic wave".
Asunto(s)
Culicidae , Entomología , Insectos Vectores , Fiebre Amarilla/epidemiología , Virus de la Fiebre Amarilla/aislamiento & purificación , Adolescente , Animales , Brasil/epidemiología , Culicidae/clasificación , Culicidae/crecimiento & desarrollo , Culicidae/microbiología , Recolección de Datos , Reservorios de Enfermedades , Agua Dulce , Haplorrinos/microbiología , Humanos , Masculino , Árboles , Fiebre Amarilla/microbiología , Fiebre Amarilla/transmisiónRESUMEN
Thirteen (8.0%) of 162 blood donors from Uberaba-Minas Gerais State showed neutralizing antibodies to the vesiculovirus Piry. The previous residence in rural area (p less than 0.0001) and in the cities of the São Paulo State (p less than 0.05) were statistically more frequent in the Piry seropositive group.
Asunto(s)
Anticuerpos Antivirales/análisis , Donantes de Sangre , Rhabdoviridae/inmunología , Adulto , Brasil , Femenino , Humanos , Masculino , Persona de Mediana Edad , Prevalencia , Rhabdoviridae/aislamiento & purificaciónRESUMEN
The authors describe the occurrence of outbreaks caused by Oropouche virus (ORO) in the states of Maranhão and Goiás, Brazil in 1988. 36 strains of the virus were obtained from the intracerebral inoculation of the blood of 120 patients into 2-3 day-old infant mice. The illness was characterized by headache, fever, pain in the muscles, joints and back, photophobia, retrobulbar pain, nausea and dizziness. 128 of 197 people examined in Porto Franco, MA, had hemagglutination-inhibiting antibodies to the agent, while 106 of them had IgM antibodies by MAC ELISA test. All age groups were infected, although the incidence was higher among who had 10 to 19 years old. There was no difference, in relation to sex infections. Recurrence of symptoms was reported in 56% of sick people. Mice inoculated with 3624 Culicoides paraensis (Ceratopogonidae) and 1970 Culex (Cux.) quinquefasciatus (Culicidae) collected in Porto Franco resulted in one single isolation of ORO virus, from the Culicoides. These are the first confirmed cases of ORO infection in Maranhão and Goiás states.
Asunto(s)
Infecciones por Bunyaviridae/epidemiología , Brotes de Enfermedades , Adolescente , Adulto , Animales , Anticuerpos Antiidiotipos/análisis , Anticuerpos Antivirales/análisis , Brasil , Niño , Culex/microbiología , Ensayo de Inmunoadsorción Enzimática , Femenino , Humanos , Inmunoglobulina M/análisis , Masculino , Persona de Mediana Edad , Virus Simbu/inmunología , Virus Simbu/aislamiento & purificaciónRESUMEN
Five new phlebotomus fever virus serotypes (Bunyaviridae: Phlebovirus) are described. These viruses, designated Ambe, Ixcanal, Mariquita, Armero, and Durania, were isolated from sand flies (Diptera: Psychodidae) collected in Brazil, Colombia, and Guatemala. Two of the agents were recovered from pools of male sand flies. The new viruses are antigenically related to other members of the phlebotomus fever serogroup by immunofluorescence, but are distinct from the other 39 members of this serogroup by plaque reduction neutralization test.
Asunto(s)
Bunyaviridae/clasificación , Phlebovirus/clasificación , Psychodidae/microbiología , Animales , Animales Recién Nacidos , Antígenos Virales/análisis , Brasil , Colombia , Femenino , Técnica del Anticuerpo Fluorescente , Guatemala , Humanos , Masculino , Ratones , Pruebas de Neutralización , Phlebovirus/inmunología , Serotipificación , Clima Tropical , Células VeroRESUMEN
280 students, between 6 and 14 years old, residents in the Iguape county, southern coast of the State of São Paulo, were studied in order to identify the existence of a possible association between the prevalence of specific antibodies to the hepatitis B virus and the exposure to haematophagous mosquitoes, evaluated indirectly through the prevalence of antibodies to 17 arboviruses isolated in Brazil. The children were from 4 areas with different topographical characteristics: 89 of the children were from the urban zone of the town of Iguape, 89 were from the peri-urban zone, 30 were from the rural area with extensive banana plantations, and 72 were from the jungle zone. Previous studies had shown significantly higher prevalence of antibodies to different arboviruses in the cultivated zone and the jungle zone, when compared to the urban and peri-urban zones of Iguape. The detection of antibodies to the HBV surface antigen (HBs Ag) was done through the radioimmunoassay (Ausab, Abbott Laboratory). The cases considered positive were confirmed through the presence of anti-core HBV antibodies (anti-HBc-EIA Roche). A significantly higher prevalence of anti-HBV antibodies was observed in children from the jungle zone (26/72 = 36.1%) when compared to those from the urban zone (5/89 = 5.6%), peri-urban (6/89 = 6.7%) or from the cultivated zone (0/30 = 0%). The result suggest the existence of a common factor in the dissemination of the arboviruses and the hepatitis B virus, supporting the hypothesis that mosquitoes may play an important role in the HBV transmission in tropical forested region.
Asunto(s)
Anticuerpos Antivirales/análisis , Arbovirus/inmunología , Virus de la Hepatitis B/inmunología , Adolescente , Animales , Infecciones por Arbovirus/transmisión , Brasil , Niño , Culicidae/microbiología , Femenino , Hepatitis B/transmisión , Anticuerpos contra la Hepatitis B/análisis , Humanos , Masculino , Clima TropicalRESUMEN
The presence of IgM antibodies to Rocio in sera of two children from rural area of Ribeira Valley, Brazil, was detected by MAC-ELISA. This new arbovirus of the Flaviviridae family was responsible for an extensive encephalitis epidemic that occurred in the region in 1975-1977. Since 1980 no human disease caused by this virus has been diagnosed. An improvement on surveillance of Rocio infections and on the researches for virus identification in suspected vectors and reservoirs is necessary.
Asunto(s)
Brotes de Enfermedades , Infecciones por Togaviridae/epidemiología , Adolescente , Anticuerpos Antivirales/análisis , Brasil , Niño , Ensayo de Inmunoadsorción Enzimática , Flavivirus/inmunología , Humanos , Inmunoglobulina M/análisis , Tamizaje Masivo , Pruebas de Neutralización , Vigilancia de la PoblaciónAsunto(s)
Antígenos Virales/análisis , Artrópodos/microbiología , Rhabdoviridae/inmunología , Vertebrados/microbiología , Animales , Pruebas de Fijación del Complemento , Reacciones Cruzadas , Ensayo de Inmunoadsorción Enzimática , Técnica del Anticuerpo Fluorescente , Humanos , Pruebas de NeutralizaciónRESUMEN
The bioecological parameters which are of special importance in the epidemiology of Dengue, Yellow Fever, and other arboviruses are discussed. Three levels are retained: the nature of Aedes aegypti-man contacts, the susceptibility of the mosquito to the pathogen and multiplication of the latter, and the transmission. The trophic preferences, the density variations, the daily survival rate, the egg diapause, and man influences are the main vector-dependent ecological factors. Temperature and genetical nature of viral and mosquito strains are particularly important in susceptibility and multiplication studies. Efficacy of the oral transmission is also temperature-dependent and mainly genetically determined. The true natural role of transovarial transmission is not yet well understood. Thus, the breaking up and/or prevention of epidemics would be possible only with a thorough knowledge of the relation between the above biological factors and the epidemiological situation. A list is provided of the naturally or experimentally Aedes aegypti transmitted arboviruses (103), protozoans (5) and filaria (20).