RESUMEN
A new, cheaper and more sensitive method for the quantitative determination of DNAase produced by S. aureus is described. The method permits the determination of DNAase activity in a wider range of titers. The method is based on the detection of the depolymerizing action of staphylococcal nuclease on DNA dyed with ethidium bromide. In this work 22 S. aureus strains isolated from monkeys and 12 strains isolated from humans have been used. The amount of produced by these strains has been determined. The DNAase results of this determination have shown that among S. aureus strains isolated from monkeys and humans the occurrence of strains with both high and low DNAase activity can be observed.
Asunto(s)
Desoxirribonucleasas/análisis , Haplorrinos/microbiología , Staphylococcus aureus/enzimología , Animales , Desoxirribonucleasas/aislamiento & purificación , Humanos , Métodos , Staphylococcus aureus/aislamiento & purificaciónRESUMEN
Protein A content in Staphylococcus aureus isolated from 6 species of monkeys at the Sukhumi Monkey Nursery has been studied. Protein A has been detected in 73% of the studied strains. One strain isolated from a rhesus macaque has been found to release high amounts of protein A into the environment.
Asunto(s)
Haplorrinos/microbiología , Proteína Estafilocócica A/análisis , Staphylococcus aureus/aislamiento & purificación , Animales , Pruebas de Hemaglutinación , Staphylococcus aureus/análisisAsunto(s)
Haplorrinos/microbiología , Staphylococcus/clasificación , Animales , Técnicas de Tipificación Bacteriana , Chlorocebus aethiops/microbiología , Femenino , Variación Genética , Macaca fascicularis/microbiología , Masculino , Staphylococcus/aislamiento & purificación , Staphylococcus/fisiologíaAsunto(s)
Coagulasa/análisis , Papio/microbiología , Staphylococcus aureus/fisiología , Enfermedades de los Animales/microbiología , Animales , Tipificación de Bacteriófagos , Femenino , Intestinos/microbiología , Masculino , Sistema Respiratorio/microbiología , Staphylococcus aureus/clasificación , Staphylococcus aureus/aislamiento & purificaciónRESUMEN
The biological properties of staphylococci isolated from 38 healthy M. rhesus and 28 dead ones were studied. Of 90 isolated staphylococcal strains, 62 proved to be S. aureus strains belonging to 6 different ecological variants.
Asunto(s)
Variación Genética , Macaca mulatta/microbiología , Macaca/microbiología , Staphylococcus aureus/fisiología , Animales , Tipificación de Bacteriófagos , Ecología , Femenino , Masculino , Infecciones Estafilocócicas/microbiología , Staphylococcus aureus/clasificación , Staphylococcus aureus/aislamiento & purificaciónRESUMEN
Cytogenetic consequence of irradiation was studied in epithelial monkey kidney cells in 3.5-9.0 years after repeated daily small dose irradiation (2.99-4.9 r every day) and in 6.0-12.5 years after a single irradiation (550-652 r). The number of reconstructed chromosomes has been found to exceed spontaneous chromosome exchanges. The reconstruction of non-stable type has been also observed. Cytogenic consequency of irradiation was due to the experiment conditions and to the total dose of irradiation.
Asunto(s)
Cromosomas/efectos de la radiación , Riñón/efectos de la radiación , Animales , Inversión Cromosómica , Radioisótopos de Cobalto , Relación Dosis-Respuesta en la Radiación , Epitelio/efectos de la radiación , Femenino , Rayos gamma , Macaca mulatta , Masculino , Ploidias , Translocación GenéticaAsunto(s)
Células Cultivadas , Mitosis , Animales , Autorradiografía , Epitelio , Haplorrinos , Riñón , Macaca mulatta , Timidina/metabolismoRESUMEN
Using 48 Macaca rhesus undergone acute single does and prolonged small dose daily irradiation, a long-term preservation of reconstructions in the pair of marker nucleolus-forming chromosomes has been shown. The animals were examined in different periods from irradiation, ranging from 3 months to 16.5 years. The frequency of retained reconstructions in the long arms of chromosomes was reliably above the frequency of alterations in the short arms. Reconstructions in the long arms of the marker chromosome were seen in the heterochromatin area after differential chromosome staining. Alterations in the functionally active area of the short arms of the marker chromosome retained extremely rarely being observed only in monkeys that survived through long-term small-dose irradiation.