Plasticity in human pitch perception induced by tonotopically mismatched electro-acoustic stimulation.

Under normal conditions, the acoustic pitch percept of a pure tone is determined mainly by the tonotopic place of the stimulation along the cochlea. Unlike acoustic stimulation, electric stimulation of a cochlear implant (CI) allows for the direct manipulation of the place of stimulation in human subjects. CI sound processors analyze the range of frequencies needed for speech perception and allocate portions of this range to the small number of electrodes distributed in the cochlea. Because the allocation is assigned independently of the original resonant frequency of the basilar membrane associated with the location of each electrode, CI users who have access to residual hearing in either or both ears often have tonotopic mismatches between the acoustic and electric stimulation. Here we demonstrate plasticity of place pitch representations of up to three octaves in Hybrid CI users after experience with combined electro-acoustic stimulation. The pitch percept evoked by single CI electrodes, measured relative to acoustic tones presented to the non-implanted ear, changed over time in directions that reduced the electro-acoustic pitch mismatch introduced by the CI programming. This trend was particularly apparent when the allocations of stimulus frequencies to electrodes were changed over time, with pitch changes even reversing direction in some subjects. These findings show that pitch plasticity can occur more rapidly and on a greater scale in the mature auditory system than previously thought possible. Overall, the results suggest that the adult auditory system can impose perceptual order on disordered arrays of inputs.

Treatment outcomes for adolescent substance abuse at 4- and 7-month assessments.

This randomized clinical trial evaluated individual cognitive-behavioral therapy (CBT), family therapy, combined individual and family therapy, and a group intervention for 114 substance-abusing adolescents. Outcomes were percentage of days marijuana was used and percentage of youths achieving minimal use. Each intervention demonstrated some efficacy, although differences occurred for outcome measured, speed of change, and maintenance of change. From pretreatment to 4 months, significantly fewer days of use were found for the family therapy alone and the combined interventions. Significantly more youths had achieved minimal use levels in the family and combined conditions and in CBT. From pretreatment to 7 months, reductions in percentage of days of use were significant for the combined and group interventions, and changes in minimal use levels were significant for the family, combined, and group interventions.

Consonant identification under maskers with sinusoidal modulation: masking release or modulation interference?

The present study investigated the effect of envelope modulations in a background masker on consonant recognition by normal hearing listeners. It is well known that listeners understand speech better under a temporally modulated masker than under a steady masker at the same level, due to masking release. The possibility of an opposite phenomenon, modulation interference, whereby speech recognition could be degraded by a modulated masker due to interference with auditory processing of the speech envelope, was hypothesized and tested under various speech and masker conditions. It was of interest whether modulation interference for speech perception, if it were observed, could be predicted by modulation masking, as found in psychoacoustic studies using nonspeech stimuli. Results revealed that masking release measurably occurred under a variety of conditions, especially when the speech signal maintained a high degree of redundancy across several frequency bands. Modulation interference was also clearly observed under several circumstances when the speech signal did not contain a high redundancy. However, the effect of modulation interference did not follow the expected pattern from psychoacoustic modulation masking results. In conclusion, (1) both factors, modulation interference and masking release, should be accounted for whenever a background masker contains temporal fluctuations, and (2) caution needs to be taken when psychoacoustic theory on modulation masking is applied to speech recognition.

Providing low- and mid-frequency speech information to listeners with sensorineural hearing loss.

The present study examined the benefits of providing amplified speech to the low- and mid-frequency regions of listeners with various degrees of sensorineural hearing loss. Nonsense syllables were low-pass filtered at various cutoff frequencies and consonant recognition was measured as the bandwidth of the signal was increased. In addition, error patterns were analyzed to determine the types of speech cues that were, or were not, transmitted to the listeners. For speech frequencies of 2800 Hz and below, a positive benefit of amplified speech was observed in every case, although the benefit provided was very often less than that observed in normal-hearing listeners who received the same increase in speech audibility. There was no dependence of this benefit upon the degree of hearing loss. Error patterns suggested that the primary difficulty that hearing-impaired individuals have in using amplified speech is due to their poor ability to perceive the place of articulation of consonants, followed by a reduced ability to perceive manner information.

Channel weights for speech recognition in cochlear implant users.

The purpose of this study was to develop and validate a method of estimating the relative "weight" that a multichannel cochlear implant user places on individual channels, indicating its contribution to overall speech recognition. The correlational method as applied to speech recognition was used both with normal-hearing listeners and with cochlear implant users fitted with six-channel speech processors. Speech was divided into frequency bands corresponding to the bands of the processor and a randomly chosen level of corresponding filtered noise was added to each channel on each trial. Channels in which the signal-to-noise ratio was more highly correlated with performance have higher weights, and conversely, channels in which the correlations were smaller have lower weights. Normal-hearing listeners showed approximately equal weights across frequency bands. In contrast, cochlear implant users showed unequal weighting across bands, and varied from individual to individual with some channels apparently not contributing significantly to speech recognition. To validate these channel weights, individual channels were removed and speech recognition in quiet was tested. A strong correlation was found between the relative weight of the channel removed and the decrease in speech recognition, thus providing support for use of the correlational method for cochlear implant users.

Disrupting defensive family interactions in family therapy with delinquent adolescents.

This study compared the immediate impact of therapist reframing, reflection, and elicit-structure interventions on family-member defensive communications in the initial session of family therapy with a delinquent adolescent. Defensive statements included family-member statements that criticized, blamed, or disagreed with other family members. Sequences of behaviors following defensive family-member statements were examined to determine which therapist interventions were the most effective in disrupting defensive family interactions. Thus, every sequence included a defensive family-member behavior, a therapist intervention, and a family-member response (sequence: family defensive-->therapist intervention-->family response). Results indicated that therapist reframing is more effective than other therapist interventions in reducing family-members' defensive statements. Moreover, adolescents responded more favorably to reframes than did fathers.

Binaural speech recognition and the Stenger effect.

The present investigation examined the effects of systematically altering the balance between speech presentation levels to the 2 ears of 12 listeners with bilateral asymmetrical sensorineural hearing impairments. Speech-recognition scores for /VCV/ speech stimuli were obtained from each participant in quiet for 9 conditions ranging from monaural poorer ear only to monaural better ear only, with 7 intermediate conditions in which the sound balance between ears was varied in 5-dB steps. High-pass spectral shaping was provided to the poorer ear, and unshaped amplification was provided to the better ear. The results suggested that, as a group, varying the sound level in the better ear within -20 to +10 dB of the centered position did not significantly change the speech recognition for these participants. No evidence of binaural interference was obtained. Findings also showed that in binaural listening situations, the Stenger effect has little influence upon speech-recognition scores. Even when the listeners were unaware of speech being presented to the better ear, their speech-recognition score reflected the better ear's abilities.

Speech audibility for listeners with high-frequency hearing loss.

This study investigated whether there are limitations on the benefit of providing audible speech information to listeners with high-frequency hearing loss. In a group of 10 listeners with various degrees of high-frequency hearing loss, speech recognition was tested across a wide range of presentation levels. For each of these listeners with hearing loss, recognition performance reached an asymptote of < 100%. When the spectrum of the speech for this asymptotic performance level was compared with the listener's pure-tone thresholds, it was seen that providing audible speech to high-frequency regions (> or = 3000 Hz), where hearing loss exceeds 55 dB HL, tended to produce little or no improvement in recognition scores. In contrast, providing audible speech to lower frequency regions for a listener with a flat, severe-to-profound hearing loss did show improvement with increasing speech audibility, despite this listener's thresholds being greater than 55 dB HL. The present study adds further support to the idea that attempting to provide amplification to regions with severe high-frequency hearing loss (> or = 3000 Hz) may not necessarily benefit many individuals with hearing loss.

Limiting spectral resolution in speech for listeners with sensorineural hearing loss.

Consonant recognition was measured as a function of the degree of spectral resolution of the speech stimulus in normally hearing listeners and listeners with moderate sensorineural hearing loss. Previous work (Turner, Souza, and Forget, 1995) has shown that listeners with sensorineural hearing loss could recognize consonants as well as listeners with normal hearing when speech was processed to have only one channel of spectral resolution. The hypothesis tested in the present experiment was that when speech was limited to a small number of spectral channels, both normally hearing and hearing-impaired listeners would continue to perform similarly. As the stimuli were presented with finer degrees of spectral resolution, and the poorer-than-normal spectral resolving abilities of the hearing-impaired listeners became a limiting factor, one would predict that the performance of the hearing-impaired listeners would then become poorer than the normally hearing listeners. Previous research on the frequency-resolution abilities of listeners with mild-to-moderate hearing loss suggests that these listeners have critical bandwidths three to four times larger than do listeners with normal hearing. In the present experiment, speech stimuli were processed to have 1, 2, 4, or 8 channels of spectral information. Results for the 1-channel speech condition were consistent with the previous study in that both groups of listeners performed similarly. However, the hearing-impaired listeners performed more poorly than the normally hearing listeners for all other conditions, including the 2-channel speech condition. These results would appear to contradict the original hypothesis, in that listeners with moderate sensorineural hearing loss would be expected to have at least 2 channels of frequency resolution. One possibility is that the frequency resolution of hearing-impaired listeners may be much poorer than previously estimated; however, a subsequent filtered speech experiment did not support this explanation. The present results do indicate that although listeners with hearing loss are able to use the temporal-envelope information of a single channel in a normal fashion, when given the opportunity to combine information across more than one channel, they show deficient performance.

Proportional frequency compression of speech for listeners with sensorineural hearing loss.

This study examined proportional frequency compression as a strategy for improving speech recognition in listeners with high-frequency sensorineural hearing loss. This method of frequency compression preserved the ratios between the frequencies of the components of natural speech, as well as the temporal envelope of the unprocessed speech stimuli. Nonsense syllables spoken by a female and a male talker were used as the speech materials. Both frequency-compressed speech and the control condition of unprocessed speech were presented with high-pass amplification. For the materials spoken by the female talker, significant increases in speech recognition were observed in slightly less than one-half of the listeners with hearing impairment. For the male-talker materials, one-fifth of the hearing-impaired listeners showed significant recognition improvements. The increases in speech recognition due solely to frequency compression were generally smaller than those solely due to high-pass amplification. The results indicate that while high-pass amplification is still the most effective approach for improving speech recognition of listeners with high-frequency hearing loss, proportional frequency compression can offer significant improvements in addition to those provided by amplification for some patients.

Administration of daniplestim and granulocyte colony-stimulating factor for the mobilization of hematopoietic progenitor cells in nonhuman primates.

We investigated the effects of administering a high-affinity interleukin-3 receptor agonist (daniplestim) and granulocyte colony-stimulating factor (G-CSF) on the mobilization of primitive hematopoietic progenitor cells into peripheral blood (PB). Groups of five rhesus monkeys were treated with 100 mg/kg of daniplestim for 5 days followed by 10 microg/kg of G-CSF for 5 days (D/G), daniplestim and G-CSF administered concurrently for 10 days (D+G), or G-CSF alone for 10 days. Phenotypic PB analysis indicated that the number of CD34+ cells in the G-CSF group had increased to 28 x 10(6)/L by Day 3 and then declined. In contrast, CD34+ cell counts of up to 68 x 10(6)/L were maintained until Day 10 in both the D/G and D+G groups. On Day 5, the total number of colony-forming cells in the PB had increased 15-fold in the D+G group and eightfold in both the D/G group and the G-CSF groups. By Day 7, the numbers of colony-forming units granulocyte/macrophage were comparable in all three groups, and 45-fold increases in the numbers of burst-forming units-erythroid and 12-fold increases in the numbers of multipotent colony-forming units were seen in both the D+G and the D/G groups. The frequency of circulating primitive progenitor cells in long-term stromal cultures was highest with D+G and lowest with G-CSF alone. These results indicate that the combination of daniplestim and G-CSF produces higher and more sustained levels of circulating stem cells than does G-CSF alone. D+G may offer advantages over D/G because it generates more long- and short-term clonogenic cells.

Quantifying the contribution of audibility to recognition of compression-amplified speech.

OBJECTIVE: The speech-recognition advantages of wide-dynamic range compression (WDRC) hearing aids are believed to be a direct result of improved audibility. The main objective of this study was to compare the relationship between increasing audibility and recognition for compression-amplified versus linearly amplified speech, using a quantitative measure of audibility. A second objective was to explore the adequacy of the Aided Articulation Index (Stelmachowicz, Lewis, Kalberer, & Creutz, 1994) in describing performance with WDRC-amplified speech. DESIGN: Sixteen listeners with mild to severe sensorineural hearing loss were tested on recognition of nonsense syllables that had been digitally processed with linear or WDRC amplification. Speech recognition scores were obtained for three input levels that differed in audibility relative to the listeners' hearing thresholds. An Aided Audibility Index (AAI), ranging from 0.0 (inaudible) to 1.0 (fully audible), was calculated for each listener and condition. This importance-weighted index represents the portion of the speech signal that is available to the listener. The relationship between audibility and recognition was then examined for each type of amplification. RESULTS: At low and moderate input levels, AAI values and corresponding recognition scores were higher for the compression-amplified than for the linearly amplified speech. At high input levels, AAIs and recognition scores were essentially the same for both types of amplification. Recognition scores increased monotonically as a function of the AAI for both linear and WDRC amplifiers. There was no significant difference between the functions for linearly amplified versus compression-amplified speech. In other words, a given increase in audibility resulted in the same increase in recognition for both types of amplification. CONCLUSIONS: Increasing the amount of audible speech information plays the same role in recognition for compression-amplified as for linearly amplified speech. This suggests that, at least for the conditions tested here, compression does not introduce detrimental changes to the speech signal that offset the benefits of improved audibility. Accuracy of the compression-amplified AAI can be improved by substituting measured speech ranges for the estimated speech ranges used in the standard AAI calculation. In the absence of measured speech ranges, the standard AAI provides a close approximation.

Does hearing aid benefit increase over time?

In a recent tutorial for the journal, Palmer et al. [J. Acoust. Soc. Am. 103, 1705-1721 (1998)] reviewed the literature on the potential for increases in hearing aid benefit over time (acclimatization). Their review might leave some readers with the impression that acclimatization has implications for the fitting and selection of hearing aids today. We (Turner and Bentler), along with two other researchers in the field (Humes and Cox), conducted a similar review of the literature a few years earlier [Turner et al., Ear and Hearing 17, 14S-28S (1996)] and found little evidence of a robust effect. The bulk of the existing evidence, including the most recent studies on this topic, support earlier conclusions, i.e., that there is no evidence for the existence of a strong acclimatization effect in current hearing aid use.

In utero transplantation of human fetal haemopoietic cells in NOD/SCID mice.

We have previously demonstrated that high levels of allogeneic, donor-derived mouse haemopoietic progenitor cells engraft following in utero transplantation in NOD/SCID mice. To evaluate whether the fetal NOD/SCID haemopoietic microenvironment supports the growth and development of human fetal haemopoietic progenitor cells, we injected fetal liver mononuclear cells (FL) or fetal bone marrow (FBM) derived CD34+ cells into NOD/SCID mice on day 13/14 of gestation. At 8 weeks of age 12% of FBM recipients and 10% of FL recipients were found to have been successfully engrafted with CD45+ human cells. CD45+ cells were present in the BM of all chimaeric animals; 5/6 recipients showed engraftment of the spleen, and 4/6 recipients had circulating human cells in the peripheral blood (PB). The highest levels of donor cells were found in the BM, with up to 15% of the nucleated cells expressing human specific antigens. Multilineage human haemopoietic engraftment, including B cells (CD19), myelomonocytic cells (CD13/33) and haemopoietic progenitor cells (CD34), was detected in the BM of chimaeric mice. In contrast, no human CD3+ cells were detected in any of the tissues evaluated. When the absolute number of engrafted human cells in the PB, BM and spleens of chimaeric mice was determined, a mean 16-fold expansion of human donor cells was observed. Although multilineage engraftment occurs in these fetal recipients, both the frequency and the levels of engraftment are lower than those previously reported when human cells are transplanted into adult NOD/SCID recipients.

Frequency-weighting functions for broadband speech as estimated by a correlational method.

The relative contributions of various regions of the frequency spectrum to speech recognition were assessed with a correlational method [K. A. Doherty and C. W. Turner, J. Acoust. Soc. Am. 100, 3769-3773 (1996)]. The speech materials employed were the 258-item set of the Nonsense Syllable Test. The speech was filtered into four frequency bands and a random level of noise was added to each band on each trial. A point biserial correlation was computed between the signal-to-noise ratio in each band on the trials and the listener's responses, and these correlations were then taken as estimates of the relative weights for each frequency band. When the four bands were presented separately, the correlations for each band were approximately equal; however, when the four bands were presented in combination, the correlations were quite different from one another, implying that in the broadband case listeners relied much more on some bands than on others. It is hypothesized that these differences reflect the way in which listeners combine and attend to speech information across various frequency regions. The frequency-weighting functions as determined by this method were highly similar across all subjects, suggesting that normal-hearing listeners use similar frequency-weighting strategies in recognizing speech.

High-frequency audibility: benefits for hearing-impaired listeners.

The present study was a systematic investigation of the benefit of providing hearing-impaired listeners with audible high-frequency speech information. Five normal-hearing and nine high-frequency hearing-impaired listeners identified nonsense syllables that were low-pass filtered at a number of cutoff frequencies. As a means of quantifying audibility for each condition, Articulation Index (AI) was calculated for each condition for each listener. Most hearing-impaired listeners demonstrated an improvement in speech recognition as additional audible high-frequency information was provided. In some cases for more severely impaired listeners, increasing the audibility of high-frequency speech information resulted in no further improvement in speech recognition, or even decreases in speech recognition. A new measure of how well hearing-impaired listeners used information within specific frequency bands called "efficiency" was devised. This measure compared the benefit of providing a given increase in speech audibility to a hearing-impaired listener to the benefit observed in normal-hearing listeners for the same increase in speech audibility. Efficiencies were calculated using the old AI method and the new AI method (which takes into account the effects of high speech presentation levels). There was a clear pattern in the results suggesting that as the degree of hearing loss at a given frequency increased beyond 55 dB HL, the efficacy of providing additional audibility to that frequency region was diminished, especially when this degree of hearing loss was present at frequencies of 4000 Hz and above. A comparison of analyses from the "old" and "new" AI procedures suggests that some, but not all, of the deficiencies of speech recognition in these listeners was due to high presentation levels.

Functional magnetic resonance imaging measures of blood flow patterns in the human auditory cortex in response to sound.

Functional Magnetic Resonance Imaging (fMRI) holds exciting potential as a research and clinical tool for exploring the human auditory system. This noninvasive technique allows the measurement of discrete changes in cerebral cortical blood flow in response to sensory stimuli, allowing determination of precise neuroanatomical locations of the underlying brain parenchymal activity. Application of fMRI in auditory research, however, has been limited. One problem is that fMRI utilizing echo-planar imaging technology (EPI) generates intense noise that could potentially affect the results of auditory experiments. Also, issues relating to the reliability of fMRI for listeners with normal hearing need to be resolved before this technique can be used to study listeners with hearing loss. This preliminary study examines the feasibility of using fMRI in auditory research by performing a simple set of experiments to test the reliability of scanning parameters that use a high resolution and high signal-to-noise ratio unlike that presently reported in the literature. We used consonant-vowel (CV) speech stimuli to investigate whether or not we could observe reproducible and consistent changes in cortical blood flow in listeners during a single scanning session, across more than one scanning session, and in more than one listener. In addition, we wanted to determine if there were differences between CV speech and nonspeech complex stimuli across listeners. Our study shows reproducibility within and across listeners for CV speech stimuli. Results were reproducible for CV speech stimuli within fMRI scanning sessions for 5 out of 9 listeners and were reproducible for 6 out of 8 listeners across fMRI scanning sessions. Results of nonspeech complex stimuli across listeners showed activity in 4 out of 9 individuals tested.

Multichannel compression, temporal cues, and audibility.

Although multichannel compression systems are quickly becoming integral components of programmable hearing aids, research results have not consistently demonstrated their benefit over conventional amplification. The present study examined two confounding factors that may have contributed to this inconsistency in results: alteration of temporal information and audibility of speech cues. Recognition of linearly amplified and multichannel-compressed speech was measured for listeners with mild-to-severe sensorineural hearing loss and for a control group of listeners with normal hearing. In addition to the standard speech signal, which provided both temporal and spectral information, the listener's ability to use temporal information in a multichannel compressed signal was directly tested using a signal-correlated noise (SCN) stimulus. This stimulus consisted of a time-varying speech envelope modulating a two-channel noise carrier. It preserved temporal cues but provided minimal spectral information. For each stimulus condition, short-term level measurements were used to determine the range of audible speech. Multichannel compression improved speech recognition under conditions where superior audibility was provided by the two-channel compression system over linear amplification. When audibility of both linearly amplified and multichannel-compressed speech was maximized, the multichannel compression had no significant effect on speech recognition score for speech containing both temporal and spectral cues. However, results for the SCN stimuli show that more extreme amounts of multichannel compression can reduce use of temporal information.

Sustained multilineage engraftment of allogeneic hematopoietic stem cells in NOD/SCID mice after in utero transplantation.

Substantial barriers exist to the engraftment of hematopoietic cells in mice after in utero transplantation. Although high levels of donor-derived hematopoiesis have been reported in SCID mice, the majority of chimeric recipients exhibit decreasing levels of donor cells over time. To directly test whether the natural killer cell and macrophage activity of the recipients represents a barrier to sustained engraftment, fetal NOD/SCID mice were injected on day 13.5 of gestation with an enriched congenic hematopoietic progenitor cell population. Forty-four percent of pups showed the presence of Ly5.1+ donor cells 4 weeks after transplantation. The mean number of donor-derived nucleated cells in the peripheral blood (PB) was 30%. Although the majority of circulating donor cells were lymphocytes, up to 15% expressed myelomonocytic markers. Serial PB samples from individual mice indicated that the percentage of circulating donor cells increased from 17% to 55% between 4 and 24 weeks. At 6 months posttransplantation, an increased frequency of multilineage, donor-derived cells was also observed in the bone marrow (BM) and the spleen of chimeric recipients. The engraftment of pluripotent hematopoietic stem cells was evaluated by transplanting BM from chimeric mice into irradiated congenic recipients. Irradiated secondary recipients also exhibited multilineage donor-derived hematopoiesis in the PB, BM, and spleen for up to 6 months. These results show that the in utero transplantation of lineage-depleted BM cells into NOD/SCID recipients produces a high frequency of sustained engraftment of allogeneic hematopoietic stem cells.

Formant transition duration and speech recognition in normal and hearing-impaired listeners.

Listeners with sensorineural hearing loss often have difficulty discriminating stop consonants even when the speech signals are presented at high levels. One possible explanation for this deficit is that hearing-impaired listeners cannot use the information contained in the rapid formant transitions as well as normal-hearing listeners. If this is the case, then perhaps slowing the rate of frequency change in formant transitions might assist their ability to perceive these speech sounds. In the present study, sets of consonant plus vowel (CV) syllables were synthesized corresponding to /ba, da, ga/ with formant transitions for each set ranging from 5 to 160 ms in duration. The listener's task was to identify the consonant in a three-alternative, closed-set response task. The results for normal-hearing listeners showed nearly perfect performance for transitions of 20 ms and longer, whereas the shortest transitions yielded poorer performance. A group of eight hearing-impaired listeners pure-tone averages (PTAs) ranging from 30 to 62 dB HL) was also tested. The hearing-impaired listeners tended to show poorer performance than the normals for transitions of all durations; however, the performance of a few hearing-impaired subjects was equal to that of normals for the shortest-duration transitions. A strong inverse relation was observed between degree of hearing loss and improvement in score as a function of transition duration. These results suggest that increasing the duration of formant transitions for listeners with more severe hearing losses may not provide a helpful solution to their speech recognition difficulties.