Protective effects of Citrullus lanatus seed ethanol extract on aluminum chloride-induced testosterone, testicular and hematological changes in an experimental male rat model.

The study was done to ascertain the protective potentials of ethanol seed extract of Citrullus lanatus on aluminum chloride-induced reproductive and hematological toxicities. Thirty mature male rats were used for the study. They were assigned into five groups (n = 6). Group 1 was treated daily with aluminum chloride (100 mg kg-1) per os for 8 weeks. Group 2 was treated with aluminum chloride (100 mg kg-1) and C. lanatus seed extract (CLSE) 200 mg kg-1 per os simultaneously for 8 weeks. Group 3 was served as a normal control and given distilled water as a placebo per os daily for 8 weeks. Group 4 was only treated with CLSE (200 mg kg-1) for eight weeks. Group 5 was only treated with aluminum chloride (100 mg kg-1) per os for 8 weeks and then treated with CLSE (200 mg kg-1) per os for another 4 weeks. Testosterone level, testicular weight, sperm motility, gonadal sperm, and extragonadal sperm reserves showed significant increases in group 2 compared to groups 1 and 5. Optimum histoarchitectural protection of the seminiferous tubules was observed in group 2, which did not differ from normal ones. For the hematological parameters, optimum protection was also observed in group 2 compared to other groups. From the results, ethanol seed extract of C. lanatus demonstrated protective potentials against aluminum's harmful effects on the male reproductive system and hematology in an experimental male rat model.

Ameliorative effects of betaine and ascorbic acid on endocrine and erythrocytic parameters of sexually-maturing female Japanese quails during the dry season.

This study investigated the ameliorative effects of betaine and ascorbic acid on some endocrine and erythrocytic parameters in female Japanese quails (Coturnix coturnix japonica) reared during the dry season. A total of 372 fourteen- day-old female quails sourced commercially was kept in cages for 56 days. After seven days acclimation, all birds were weighed and allotted by complete random design to four groups with 3 replicates per group. Every group having 93 quails, comprised of 31 birds per replicate. Experimental groups were birds fed: Control (basal); ascorbic acid (AA), at 200 mg/Kg; betaine (BET) at 2 g/kg and combination of AA (200 mg/Kg) + BET (2 g/kg) of diets. Daily dry-bulb temperature (DBT), relative humidity (RH) and temperature-humidity index (THI) measured at 08:00 h, 13:00 h and 17:00 h fluctuated widely and exceeded the zone of thermal comfort for Japanese quails. Serum levels of catalase (CAT), reduced glutathione (GSH), cortisol, sex hormones (luteinizing hormone, LH and estradiol) and erythrocyte parameters (packed cell volume, PCV; red blood count, RBC; haemoglobin concentration, Hb; mean corpuscular volume (MCV), hemoglobin (MCH) were obtained at 28, 49 and 70 days of age. In female quails, AA ± BET increased (P < 0.05) CAT and GSH, but decreased (P < 0.05) cortisol levels when compared with control values at varying ages. There were higher (P < 0.05) values of LH in quails fed dietary AA + BET (28 and 49 day-old) and estradiol in those which consumed either BET or AA + BET (28, 49 and 70 day-old) and AA (at 70 day-old). At 49 day-old, either BET or AA + BET increased (P < 0.05) RBC count, but lowered (P < 0.05) MCV and MCH. In conclusion, betaine and ascorbic acid supplementation improved activities of serum sex and stress hormones, and erythrocytic parameters of Japanese quails during the dry season.

Effects of betaine and ascorbic acid supplementation on serum gonadotropin, testicular histological analysis and sperm quality in male Japanese quails during the dry season.

The study investigated the effects of betaine and ascorbic acid supplementation on serum gonadotropin, testicular histological features and sperm quality in male Japanese quails reared during the dry season. Two hundred and forty 14 day-old male Japanese quails, obtained commercially and housed in cages, were assigned randomly to four dietary treatments for 56 days. Each treatment comprised of 3 replicates (20 birds per replicate). Birds in Control group were fed only basal diet, while those in AA group consumed supplemental ascorbic acid at (200 mg/kg); BET group, betaine (2 g/kg) and AA + BET, a combination of AA (200 mg/kg) and betaine (2 g/kg) in diets. Feeds and water were offered to all birds ad libitum. The prevailing environmental conditions during the study predominantly exceeded the zone of thermal comfort for Japanese quails with DBT ranging from 25.0 to 37.0 °C; RH, 48.0-92.0% and THI, 69.8-91.0. At 28, 49 and 70 day-old, serum concentrations of malondialdhyde (MDA), superoxide dismutase (SOD), gonadotropins [luteinizing hormone (LH) and follicle stimulating hormones (FSH)] and testicular histological analyses were evaluated. Sperm quality was assessed in 70 day - old quails as sperm motility (SM), vitality (SV), total abnormalities (STA) and reserve (SR). Supplemental AA + BET decreased (P < 0.05) MDA, but improved (P < 0.05) SOD in 28, 49 and 70 day-old quails. Serum LH and FSH activities were enhanced (P < 0.05) in birds fed AA ± BET diets, but those which consumed BET or AA + BET showed enhanced (P < 0.05) testicular histological architecture and spermatogenic activities. Birds fed AA ± BET showed improved SM, SV and SR (P < 0.05), but lower STA in 70 days old male quails. It is concluded that supplementation of betaine and ascorbic acid enhances male reproductive potentials in Japanese quails during the dry season.