RESUMEN
Uganda is the only country in sub-Saharan Africa whose onchocerciasis elimination programme extensively uses vector control and biannual treatment with ivermectin. The purpose of this study was to assess the impact of combined strategies on interrupting onchocerciasis transmission in the Kashoya-Kitomi focus. Mass Drug Administration annually (13 years) followed by biannual treatments (6 years) and ground larviciding (36 cycles in 3 years) with temephos (Abate®, EC500) against Simulium neavei were conducted. Routine fly catches were conducted for over seven years in six catching sites and freshwater crabs Potamonautes aloysiisabaudiae were examined for immature stages of Simulium neavei. Epidemiological assessments by skin snip were performed in 2004 and 2013. Collection of dry blood spots (DBS) from children <10 years for IgG4 antibodies analysis were done in 2010 and 2013. Treatment coverage with ivermectin improved with introduction of biannual treatment strategy. Microfilaria prevalence reduced from 85% in 1991 to 62% in 2004; and to only 0.5% in 2013. Crab infestation reduced from 59% in 2007 to 0% in 2013 following ground larviciding. Comparison of total fly catches before and after ground larviciding revealed a drop from 5334 flies in 2007 to 0 flies in 2009. Serological assays conducted among 1,362 children in 2010 revealed 11 positive cases (0.8%; 95% CI: 0.4%-1.2%). However, assessment conducted on 3246 children in 2013 revealed five positives, giving point prevalence of 0.15%; 95% CI: 0.02%-0.28%. Four of the five children subjected to O-150 PCR proved negative. The data show that transmission of onchocerciasis has been interrupted based on national and WHO Guidelines of 2012 and 2016, respectively.
Asunto(s)
Antiparasitarios/uso terapéutico , Control de Insectos/métodos , Insecticidas , Oncocercosis/prevención & control , Animales , Niño , Humanos , Insectos Vectores , Ivermectina/uso terapéutico , Microfilarias/efectos de los fármacos , Onchocerca volvulus , Oncocercosis/transmisión , Simuliidae/efectos de los fármacos , Temefós , Uganda/epidemiologíaRESUMEN
Many blackfly species (Diptera: Simuliidae) are economically important insect pests, both as nuisance biters and as vectors of pathogens of medical and veterinary relevance. Among the important blackfly pest species in North America is Simulium vittatum Zetterstedt sensu lato. The objective of this study was to identify compounds excreted by mammalian hosts that are attractive to host-seeking S. vittatum females. The attractiveness of putative compounds to colonized S. vittatum was tested through electrophysiological (electroantennography; n = 58 compounds) and behavioural (Y-tube assays; n = 7 compounds in three concentrations) bioassays. Five compounds were significantly attractive to host-seeking S. vittatum females: 1-octen-3-ol; 2-heptanone; acetophenone; 1-octanol, and naphthalene. These candidate compounds might be useful as attractants in traps that could be developed for use in alternative or complementary management tactics in programmes to suppress nuisance blackfly populations, or for the collection of samples in which to study the transmission ecology of pathogens transmitted by blackflies of the S. vittatum complex.
Asunto(s)
Quimiotaxis , Insectos Vectores/efectos de los fármacos , Insectos Vectores/fisiología , Feromonas/farmacología , Simuliidae/efectos de los fármacos , Simuliidae/fisiología , Animales , FemeninoRESUMEN
Ivermectin resistance is common in trichostrongylid nematodes of livestock, such as Haemonchus contortus. This anthelmintic is the only drug approved for mass administration to control onchocerciasis caused by the nematode parasite, Onchocerca volvulus. In parts of West Africa up to 18 rounds of ivermectin treatment have been administered to communities and there are reports of poor parasitological responses to treatment. Understanding ivermectin resistance and ivermectin selection is an important step to reduce selection pressure for resistance, and to develop molecular markers which can be used to monitor the development of resistance and its spread. Here we report evidence that ivermectin selection changes the frequency of beta-tubulin alleles in both the sheep parasite, H. contortus, and the human parasite, O. volvulus. In O. volvulus we have been able to look at the frequency of beta-tubulin alleles in O. volvulus obtained before any ivermectin was used in humans in Africa, and following its widespread use. In H. contortus, we have been able to look at the frequency of beta-tubulin alleles in a strain which has not seen any anthelmintic selection and in an ivermectin selected strain derived from the unselected strain. We have found ivermectin selects on beta-tubulin in both of these nematode species. In the case of O. volvulus, we had previously reported that ivermectin selects for specific single nucleotide polymorphisms in the O. volvulus beta-tubulin gene. This polymorphism results in three amino acid changes in the H3 helix of beta-tubulin, as well as deletions in an associated intron. We report a simple PCR assay to detect the amplicon length polymorphism, resulting from these intronic deletions, which can be used to monitor the frequency of the beta-tubulin allele selected for by ivermectin in O. volvulus.
Asunto(s)
Resistencia a Medicamentos , Filaricidas/uso terapéutico , Hemoncosis/tratamiento farmacológico , Haemonchus/efectos de los fármacos , Ivermectina/uso terapéutico , Onchocerca volvulus/efectos de los fármacos , Oncocercosis/tratamiento farmacológico , Tubulina (Proteína)/genética , África Occidental , Animales , Filaricidas/farmacología , Frecuencia de los Genes , Hemoncosis/parasitología , Haemonchus/genética , Humanos , Ivermectina/farmacología , Microfilarias/genética , Microfilarias/aislamiento & purificación , Onchocerca volvulus/genética , Onchocerca volvulus/crecimiento & desarrollo , Oncocercosis/parasitología , Polimorfismo de Longitud del Fragmento de Restricción , Polimorfismo Conformacional Retorcido-Simple , Ovinos/parasitología , Piel/patologíaRESUMEN
The species composition and population dynamics of adult mosquitoes in a wetland near Iuka, MS, were analyzed over a 6-yr period (1997-2002) and reverse transcription-polymerase chain reaction (PCR) detection rates of arboviruses determined during five of those years. Blood meals of three likely vector species were identified using a PCR-based method that allows identification of the host to species. Culex erraticus (Dyar & Knab) composed 51.9% of the population during the 6-yr period with 295 females collected per trap night. Eastern equine encephalomyelitis (EEE) virus was detected in six genera of mosquitoes [Coquillettidia perturbans (Walker), Culex restuans Theobald, Culex salinarius Coquillett, Culex erraticus (Dyar & Knab), Anopheles crucians Wiedemann, Anopheles quadrimaculatus Say, Aedes vexans (Meigen), Ochlerotatus triseriatus Say, and Psorophora ferox Humboldt) with positive pools occurring in 1998, 1999, and 2002. Culiseta melanura Coquillett occurred at a low level (< 1%) and was not infected. Saint Louis encephalitis virus was detected once in a single pool of Cx. erraticus in 1998. Neither West Nile virus nor LaCrosse virus was found. Minimum infection rates per 1000 females tested of competent vectors of EEE virus were variable and ranged from 0.14 for Cx. erraticus to 40.0 for Oc. triseriatus. Thirty-nine species of birds were identified in the focus with blood-engorged mosquitoes found to contain meals (n = 29) from eight avian species. The majority of meals was from the great blue heron, Ardea herodias L. (n = 55%), but when bird abundance data were adjusted for avian mass, the brown-headed cowbird, Molothrus ater (Boddaert); blue jay, Cyanocitta cristata (L.); and northern mockingbird, Mimus polyglottos (L.), were overrepresented as hosts.
Asunto(s)
Arbovirus/aislamiento & purificación , Culex , Culicidae , Animales , Arbovirus/clasificación , Arbovirus/genética , Secuencia de Bases , Aves/parasitología , Clima , Culex/fisiología , Culex/virología , Culicidae/fisiología , Culicidae/virología , Cartilla de ADN , Ambiente , Conducta Alimentaria , Mississippi , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
Onchocerca volvulus exists in at least two strains in West Africa, while its black-fly vectors consist of sibling species, dwelling in the savanna and forest/transition zones. In transition and degraded forest zones both parasite strains and different sibling species of the vector can be sympatric. The strain of parasite in infected humans and in vector black-flies was determined in two bioclimes along the Bandama river of Côte d'Ivoire. The upper Bandama is located in the savanna bioclime while the Middle Bandama is located in a degraded forest zone. At both sites, savanna-dwelling sibling species of the Simulium damnosum sensu lato species complex predominated. The severe-strain of O. volvulus was the predominant strain at both sites. However, severe-strain parasites represented a significantly larger proportion of those found in the vector population than in the human population in the degraded forest of the Middle Bandama. These data suggest that in degraded forest areas recently invaded by savanna-dwelling species of S. damnosunz s.l. transmission of the severe-strain of the parasite might be more efficient than transmission of the mild-strain.
Asunto(s)
Insectos Vectores/parasitología , Onchocerca volvulus/crecimiento & desarrollo , Oncocercosis/parasitología , Simuliidae/parasitología , Animales , Clima , Côte d'Ivoire/epidemiología , ADN de Helmintos/química , ADN de Helmintos/genética , Ecosistema , Humanos , Onchocerca volvulus/genética , Oncocercosis/epidemiología , Reacción en Cadena de la Polimerasa , Simuliidae/anatomía & histologíaRESUMEN
Deletion and insertion mutagenesis was used to analyze the targeting sequence of the nuclear encoded apicoplast protein, the ribosomal protein small subunit 9 of Toxoplasma gondii. Previous studies have shown that nuclear encoded apicoplast proteins possess bipartite leaders having characteristic signal sequences followed by serine/threonine rich transit sequences. Deletion analysis demonstrated that the first 55 amino acids of the rps9 leader were sufficient for apicoplast targeting. Insertional mutagenesis tagging the leader sequence with a hemagglutinin (HA) tag was used to study the events involved in the targeting pathway. Transfectants with insertions near the N-terminus of the transit displayed HA tagged precursors outside of the apicoplast, in the perinuclear region. In contrast, transfectants with the HA tag inserted near the carboxyl end of the transit-like region had apicoplast labeling. Western blot analysis of HA tagged stable isolates suggested that processing of the HA tagged leaders was a multi-step process, with processing occurring both outside of and at or within the apicoplast.
Asunto(s)
Péptidos/metabolismo , Plastidios/genética , Plastidios/metabolismo , Señales de Clasificación de Proteína , Proteínas Protozoarias/metabolismo , Proteínas Ribosómicas/metabolismo , Toxoplasma/genética , Animales , Western Blotting , Línea Celular , Electroporación , Eliminación de Gen , Humanos , Microscopía Fluorescente , Mutagénesis Insercional , Péptidos/genética , Plásmidos/genética , Subunidades de Proteína , Transporte de Proteínas , Proteínas Protozoarias/genética , Proteínas Ribosómicas/genética , Toxoplasma/crecimiento & desarrollo , Toxoplasma/metabolismo , TransfecciónRESUMEN
The cuticle of parasitic nematodes consists primarily of a network of collagen molecules. The enzyme responsible for collagen maturation is prolyl 4-hydroxylase, making this enzyme a central activity in cuticle biosynthesis and a potentially important chemotherapeutic target. Adult and embryonic Brugia malayi are shown to be susceptible to inhibitors of vertebrate prolyl 4-hydroxylase, with exposed parasites exhibiting pathologies consistent with a disruption in cuticle biosynthesis. A full-length cDNA (Ov-phy-1) encoding a catalytically active alpha-subunit of Onchocerca volvulus prolyl 4-hydroxylase was isolated and characterized. The derived amino acid sequence of Ov-phy-1 encoded a peptide that was most similar to the two Caenorhabditis elegans prolyl 4-hydroxylase homologues and to the isoform II enzymes of vertebrates. Expressed sequence tag (EST) analysis and developmental polymerase chain reaction (PCR) studies demonstrated that Ov-phy-1 was expressed in L3 and adult parasites. The gene encoding the Ov-phy-1 open reading frame contained 11 introns, similar in structure to the gene encoding human prolyl 4-hydroxylase isoform I. Genomic Southern blot, EST and genomic PCR studies demonstrated that the O. volvulus genome contained between three and eight genes closely related to Ov-phy-1. Co-expression of Ov-phy-1 with the O. volvulus homologue of protein disulfide isomerase in a baculovirus system resulted in the production of enzymatically active O. volvulus prolyl 4-hydroxylase. In vitro production of enzymatically active O. volvulus prolyl 4-hydroxylase should facilitate identification of specific inhibitors of the parasite enzyme.
Asunto(s)
Genes de Helminto , Onchocerca volvulus/genética , Procolágeno-Prolina Dioxigenasa/genética , Secuencia de Aminoácidos , Animales , Southern Blotting , Brugia Malayi/efectos de los fármacos , Inhibidores Enzimáticos/farmacología , Femenino , Humanos , Isoenzimas/genética , Datos de Secuencia Molecular , Onchocerca volvulus/embriología , Onchocerca volvulus/enzimología , Sistemas de Lectura Abierta , Filogenia , Reacción en Cadena de la Polimerasa , Procolágeno/metabolismo , Procolágeno-Prolina Dioxigenasa/antagonistas & inhibidores , Procolágeno-Prolina Dioxigenasa/clasificación , Pironas/farmacología , Proteínas Recombinantes/metabolismo , Alineación de SecuenciaRESUMEN
Pneumocystis carinii pneumonia (PCP) remains the most common opportunistic infection among human immunodeficiency virus-infected persons. Despite this, little is known concerning the transmission dynamics of this infection. In the absence of a reliable method to isolate and culture P. carinii from environmental samples, it has not been possible to assess the importance of person-to-person transmission in the epidemiology of PCP. A molecular viability assay was developed for the human form of P. carinii (P. carinii f sp hominis) that is applicable to both clinical specimens and environmental samples. This assay will enable the evaluation of the spread and persistence of viable human P. carinii in the environment.
Asunto(s)
Infecciones Oportunistas Relacionadas con el SIDA/microbiología , ADN Bacteriano/análisis , Pneumocystis/genética , Neumonía por Pneumocystis/microbiología , Infecciones Oportunistas Relacionadas con el SIDA/genética , Adulto , Líquido del Lavado Bronquioalveolar/microbiología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Datos de Secuencia Molecular , Pneumocystis/aislamiento & purificación , Neumonía por Pneumocystis/epidemiología , Neumonía por Pneumocystis/transmisión , ARN Mensajero/análisis , ARN Ribosómico 18S , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Análisis de Secuencia de ADN , Esputo/microbiologíaRESUMEN
Onchocerciasis in Sudan exists in three distinct foci which exhibit differing clinical presentations. Previous studies have demonstrated that a tandemly repeated Onchocerca sequence family with a unit repeat length of 150 bp (the O-150 family) is a useful marker for deducing relationships among different O. volvulus populations. In the current study, the O-150 repeat families of O. volvulus from Sudan were analyzed and compared to each other and to those of parasites from West Africa. Similar to West African and American O. volvulus, the O-150 families of the Sudanese parasites could be divided into clusters within which little or no intracluster variation was evident, suggesting that the O-150 family in these parasites was subject to the forces of concerted evolution. Statistical analysis of the O-150 families from the different Sudanese parasite isolates, employing a nested algorithm based on an analysis of variance, revealed that O. volvulus endemic to the northern focus at Abu Hamed were significantly different from all other O. volvulus populations examined to date. In contrast, parasites from the southern and eastern foci of Sudan were indistinguishable from those endemic to the West African savanna. The significance of these data are discussed in light of knowledge of the biogeography and biology of transmission of O. volvulus in Africa.
Asunto(s)
Variación Genética , Onchocerca volvulus/genética , Oncocercosis/parasitología , África Occidental , Análisis de Varianza , Animales , Secuencia de Bases , Clonación Molecular , Análisis por Conglomerados , Secuencia de Consenso , ADN de Helmintos/química , Humanos , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , Alineación de Secuencia , Sudán , Secuencias Repetidas en Tándem , YemenRESUMEN
PCR detection methods are useful in studies of organisms not amenable to culture. Inhibitors in environmental samples can interfere with such assays. We describe a magnetic bead DNA capture protocol that removes inhibitors from outdoor air samples, maintaining the sensitivity of a 16S Pneumocystis carinii mitochondrial rRNA gene-based PCR.
Asunto(s)
Microbiología del Aire , ADN de Hongos/análisis , Monitoreo del Ambiente/métodos , Pneumocystis/aislamiento & purificación , Reacción en Cadena de la Polimerasa/métodos , Filtración , Magnetismo , Pneumocystis/genética , Cemento de Policarboxilato , Sensibilidad y EspecificidadRESUMEN
Thirteen-year cicadas of brood XIX from northern Arkansas, Missouri, and southern Illinois (lineage A) are known to be genetically different at two marker loci (mitochondrial DNA and abdominal color) from 13-year cicadas to the south (lineage B) that emerge in the same year. Because 17-year cicadas from all broods (year classes) are indistinguishable from lineage A at these two marker loci, previous workers suggested that the lineage A cicadas of 13-year brood XIX were derived from 17-year cicadas by life-cycle switching (allochrony). Data presented here show that, over the same northern geographic range, lineage A is also present in 13-year cicadas belonging to brood XXIII (which always emerges four years later than brood XIX). Detailed sampling along the putative life-cycle-switching boundary in 13-year brood XXIII revealed a previously unsuspected broad zone of overlap where populations contained individuals of both lineages A and B. Despite this sympatry, and previous reports of a lack of behavioral barriers to interbreeding, a strong correlation between mitochondrial haplotype and abdominal color suggests that assortative mating has taken place. Lineage A 13-year cicadas from both broods XIX and XXIII are only found within a gap in the spatial distribution of 17-year cicadas. This, in combination with the lack of differentiation between lineage A 13- and 17-year cicadas at the marker loci and new behavioral data for 13-year brood XIX, suggests a recent derivation of all northern 13-year cicadas from the 17-year cicadas via life-cycle switching. We discuss the implications of these allochronic shifts for speciation.
Asunto(s)
Relojes Biológicos/genética , Evolución Biológica , Hemípteros/clasificación , Hemípteros/genética , Periodicidad , Conducta Sexual Animal , Animales , Conducta de Elección , ADN Mitocondrial/genética , Evolución Molecular , Femenino , Geografía , Hemípteros/fisiología , Estadios del Ciclo de Vida , Masculino , Datos de Secuencia Molecular , Filogenia , Reproducción , Especificidad de la Especie , Estados UnidosRESUMEN
The northernmost focus for Onchocerca volvulus Leuckhart (Nematoda: Onchocercidae), the causative agent of human onchocerciasis, is found along the Nile near the town of Abu Hamed in Sudan. The vector for O. volvulus at this focus is a single monomorphic population of Simulium (Edwardsellum) damnosum Theobald. This black fly population is limited to a small area between the fourth and fifth cataracts of the Nile River that is isolated geographically from all other populations of S. damnosum sensu lato. Phylogenies produced from cytological analyses and sequence data derived from the NADH dehydrogenase subunit 4 and 16S rRNA genes indicate that Abu Hamed black flies are similar to, but distinct from, the savanna-dwelling sibling species of S. damnosum s.l., Simulium (Edwardsellum) damnosum sensu strictu Theobald, and S. (Edwardsellum) sirbanum Vajime & Dunbar. The DNA sequence and the cytological data support the hypothesis that the black fly population present in Abu Hamed may represent a new sibling species of S. damnosum s.l. We propose that this population be informally designated as the hamedense form of the Simulium damnosum complex.
Asunto(s)
Simuliidae/clasificación , Animales , Clasificación , Femenino , Genes de Insecto , Humanos , NADH Deshidrogenasa/genética , ARN Ribosómico 16S/análisis , Simuliidae/enzimología , Simuliidae/genética , SudánRESUMEN
Although the mechanisms underlying the host inflammatory response in ocular onchocerciasis have been examined, the role of particular parasite proteins in this process remains largely unexplored. Recently, it was found that one of the most abundant expressed sequence tags in Onchocerca volvulus infective larvae encoded a protein with similarities to a component of vespid venom. This clone was designated O. volvulus Activation associated Secreted Protein -1 (Ov-asp-1). We report the characterization of three members of a family of proteins, designated the Ov-ASP family, of which Ov-ASP-1 is a member. Sequence based and phylogenetic analyses suggest that these proteins form a filarial specific protein family related to both the vespid venom antigen 5 and the vertebrate CRISP/Tpx family of proteins. The three members of the Ov-ASP family exhibit distinct patterns of expression in the life cycle of O. volvulus. Genomic Southern blot analyses indicate that several genes encoding sequences related to the Ov-asp family are present in the genome of O. volvulus. Recombinant proteins expressed from full length cDNAs encoding two members of the Ov-asp family were found to induce an angiogenic response after injection into corneas of naive mice, and vessel formation was associated with only minor inflammatory cell infiltration. These data suggest that Ov-ASP proteins may directly induce an angiogenic response and may therefore contribute to corneal neovascularization in onchocercal keratitis.
Asunto(s)
Antígenos Helmínticos , Proteínas del Helminto/fisiología , Neovascularización Patológica , Onchocerca volvulus/patogenicidad , Oncocercosis Ocular/parasitología , Venenos de Avispas/genética , Animales , Córnea/irrigación sanguínea , Proteínas del Helminto/química , Proteínas del Helminto/genética , Proteínas del Helminto/farmacología , Ratones , Ratones Endogámicos BALB C , Datos de Secuencia Molecular , Onchocerca volvulus/metabolismo , Oncocercosis Ocular/patología , Filogenia , Proteínas Recombinantes de Fusión/farmacología , Análisis de Secuencia de ADN , Venenos de Avispas/metabolismoRESUMEN
Despite recent declines in incidence, Pneumocystis carinii pneumonia (PCP) remains the most commonly occurring opportunistic illness among persons with AIDS in the United States. While P. carinii DNA has been detected in patient respiratory specimens and in air samples collected from various indoor environments housing PCP patients, the viability of these organisms is unknown. For this reason, we have developed and evaluated a molecular viability assay for P. carinii. This method is based upon the detection of P. carinii mRNA by a reverse transcription-PCR that employs specific primers from a member of the heat shock protein 70 family. Under optimal assay conditions, these primers were capable of detecting as few as 100 viable trophozoites as determined by ethidium bromide staining, while no signal was obtained from 10(6) trophozoites killed by heat, desiccation, or UV radiation. This assay was also capable of distinguishing P. carinii from other common fungi present in the air. Therefore, this molecular viability assay may be useful in conjunction with standard bioaerosol collection devices and procedures for the detection of viable P. carinii collected from various indoor environments. It may also be useful in confirming the presence of viable trophozoites in respiratory specimens collected by noninvasive techniques from putatively infected individuals.
Asunto(s)
Pneumocystis/genética , Pneumocystis/aislamiento & purificación , Contaminación del Aire Interior , Secuencia de Bases , Southern Blotting , Humanos , Datos de Secuencia Molecular , Pneumocystis/fisiología , Neumonía por Pneumocystis/transmisión , ARN de Hongos/análisis , ARN Mensajero/análisis , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Sensibilidad y EspecificidadRESUMEN
Onchocerca volvulus, the filarial parasite that causes onchocerciasis or river blindness, contains three distinct genomes. These include the nuclear genome, the mitochondrial genome and the genome of an intracellular endosymbiont of the genus Wolbachia. The nuclear genome is roughly 1.5x10(8) bp in size, and is arranged on four chromosome pairs. Analysis of expressed sequence tags from different life-cycle stages has resulted in the identification of transcripts from roughly 4000 O. volvulus genes. Several of these transcripts are highly abundant, including those encoding collagen and cuticular proteins. Analysis of several gene sequences from O. volvulus suggests that the nuclear genes of O. volvulus are relatively compact and are interrupted relatively frequently by small introns. The intron-exon boundaries of these genes generally follow the GU-AG rule characteristic of the splice donor and acceptors of other vertebrate organisms. The nuclear genome also contains at least one repeated sequence family of a 150 bp repeat which is arranged in tandem arrays and appears subject to concerted evolution. The mitochondrial genome of O. volvulus is remarkably compact, only 13747 bp in size. Consistent with the small size of the genome, four gene pairs overlap, eight contain no intergenic regions and the remaining gene pairs are separated by small intergenic domains ranging from 1 to 46 bp. The protein-coding genes of the O. volvulus mitochondrial genome exhibit a striking codon bias, with 15/20 amino acids having a single codon preference greater than 70%. Intraspecific variation in both the nuclear and mitochondrial genomes appears to be quite limited, consistent with the hypothesis that O. volvulus has suffered a genetic bottleneck in the recent past.
Asunto(s)
Genoma , Onchocerca volvulus/genética , Animales , ADN Mitocondrial/química , Expresión Génica , Variación GenéticaRESUMEN
The Onchocerciasis Control Programme in West Africa (OCP) has succeeded in eliminating blinding onchocerciasis as a public health problem throughout much of West Africa. The efforts of the OCP are now turning towards surveillance, with the goal of rapidly detecting and controlling outbreaks of infection in the onchocerciasis-free zone. With this goal in mind, cutaneous application of a solution of diethylcarbamazine (the DEC-patch test) was evaluated in 1996-99 as a method to detect patent Onchocerca volvulus infection in children and adolescents, a sentinel population for the detection of recrudescence. In an analysis of 1887 individuals in Côte d'Ivoire and Burkina Faso, the DEC-patch test produced prevalence estimates comparable to those obtained by skin snip. The sensitivity of the DEC-patch assay was marginally greater in children and adolescents than in adults, and was greater in individuals who had received prior Mectizan treatment. These data suggest that the DEC-patch test may be a useful tool for detecting recrudescence of O. volvulus infection in a sentinel population of children and young adults within the onchocerciasis-free zone created by the OCP.
Asunto(s)
Dietilcarbamazina , Filaricidas , Oncocercosis Ocular/diagnóstico , Administración Cutánea , Adolescente , Adulto , Niño , Dietilcarbamazina/administración & dosificación , Filaricidas/administración & dosificación , Humanos , Reacción en Cadena de la Polimerasa/normas , Recurrencia , Sensibilidad y Especificidad , Pruebas Cutáneas/normasRESUMEN
West African populations of Onchocerca volvulus endemic to the rain forest and savanna bioclimes of West Africa differ in their ability to induce ocular disease in infected individuals. In recent years, both clinical- and animal-model-based studies have implicated particular parasite antigens in the development of ocular onchocerciasis. To test the hypothesis that the difference in pathogenic potential of blinding and nonblinding parasites might be reflected in qualitative differences in antigens that have been implicated in the development of ocular onchocerciasis, we compared the sequences of two parasite antigens implicated in the development of ocular disease in blinding- and nonblinding-strain parasites. The results demonstrated a high level of homogeneity between the parasite strains in these genes. The study was extended to include additional nuclear genes encoding antigens that are commonly recognized by individuals infected with O. volvulus and to the mitochondrial genome of the parasite. The results demonstrate a high degree of homogeneity in both the nuclear and the mitochondrial genomes among O. volvulus isolates collected from several different sites in Africa and in the Americas. This high degree of genetic homogeneity may reflect the passage of the parasite through a recent genetic bottleneck.
Asunto(s)
Antígenos Helmínticos/genética , Heterogeneidad Genética , Genoma , Onchocerca volvulus/genética , Oncocercosis Ocular/parasitología , África , Américas , Animales , Antígenos Helmínticos/química , Secuencia de Bases , Núcleo Celular/química , ADN Complementario/química , ADN de Helmintos/química , ADN Mitocondrial/química , Epítopos/química , Epítopos/genética , Variación Genética , Proteínas del Helminto/química , Proteínas del Helminto/genética , Humanos , Datos de Secuencia Molecular , Onchocerca volvulus/inmunología , Onchocerca volvulus/patogenicidad , Oncocercosis Ocular/inmunología , Reacción en Cadena de la Polimerasa , Polimorfismo de Longitud del Fragmento de Restricción , Alineación de Secuencia , VirulenciaRESUMEN
Steroids and retinoids are important regulators of development in invertebrates and vertebrates. The central mediators of action of these compounds are their cognate receptors, which together form a family of proteins known as the nuclear receptor family. Previous studies have demonstrated that the genome of Onchocerca volvulus encodes at least three members of the nuclear receptor family. Here, the characterization of one member of this family from O. volvulus, designated OvNR-2, is described. OvNR-2 was found to be most similar to a number of vertebrate retinoic acid receptors and to the Drosophila melanogaster EiP78c protein. Modeling studies suggest that OvNR-2 forms a boot shaped ligand-binding cavity of a shape and size that can bind steroids. Expression of the mRNA corresponding to OvNR-2 is tightly regulated in adult parasites, appearing only in the extended intrauterine microfilariae. The protein derived from expression of the OvNR-2 cDNA in a bacterial system is recognized by serum antibodies in a majority of individuals infected with O. volvulus.
Asunto(s)
Onchocerca volvulus/genética , Receptores Citoplasmáticos y Nucleares/química , Receptores Citoplasmáticos y Nucleares/genética , Secuencia de Aminoácidos , Animales , ADN Complementario/aislamiento & purificación , Femenino , Hibridación in Situ , Modelos Moleculares , Datos de Secuencia Molecular , Onchocerca volvulus/metabolismo , Filogenia , Conformación Proteica , ARN de Helminto/metabolismo , ARN Mensajero/metabolismo , Receptores Citoplasmáticos y Nucleares/metabolismo , Alineación de Secuencia , Transcripción GenéticaRESUMEN
We developed a DNA assay for bloodmeal identification in haematophagous insects. Specific host cytochrome B gene sequences were amplified by PCR and classified on the basis of their mobility in a heteroduplex assay. In the blackfly Simulium damnosum s.l. (Diptera: Simuliidae), human cytochrome B DNA sequences were identifiable up to 3 days following ingestion of the bloodmeal. In the tsetse Glossina palpalis (Diptera: Glossinidae) collected from tsetse traps in Ivory Coast, bloodmeals were identified as taken from domestic pigs on the basis of their heteroduplex pattern and DNA sequence. Evidently the cytochrome B sequence shows sufficient interspecific variation to distinguish between mammalian host samples, while exhibiting minimal intraspecific variation. The stability of DNA in bloodmeals, for several days post-ingestion by haematophagous insects, allows PCR-HDA assays to be used reliably for host identification.
Asunto(s)
Culex , Grupo Citocromo b/genética , Ácidos Nucleicos Heterodúplex , Simuliidae , Moscas Tse-Tse , Animales , Sangre , Bovinos , Femenino , Humanos , Ratones , Sigmodontinae , PorcinosRESUMEN
In persons with onchocerciasis, topical application of the anthelminthic diethylcarbamazine (DEC) induces clinical and histologic responses similar to acute papular onchodermatitis, including recruitment of eosinophils to the skin. To determine whether the eosinophil chemokine eotaxin is likely to be associated with eosinophil recruitment in onchodermatitis, DEC was applied to a 5-cm2 area on the skin of infected persons, and biopsies were taken from lesions 24 h later. Histologic analysis showed elevated dermal and epidermal eosinophils compared with tissue from an adjacent (untreated) site. Reverse transcription-polymerase chain reaction showed that eotaxin gene expression in DEC-treated skin was elevated 2- to 17-fold compared with control tissue. Eotaxin immunoreactivity was noted in mononuclear cells and eosinophils in the perivascular region of the dermis and in lymphatic and vascular endothelial cells. Together, these observations are consistent with a role for eotaxin in recruitment of eosinophils to the dermis in early stage onchocercal skin disease.