Asunto(s)
Compuestos de Metilmercurio/toxicidad , Nervio Trigémino/efectos de los fármacos , Fosfatasa Ácida/metabolismo , Adenosina Trifosfatasas/metabolismo , Fosfatasa Alcalina/metabolismo , Animales , Masculino , Intoxicación por Mercurio/enzimología , Ratas , Succinato Deshidrogenasa/metabolismo , Nervio Trigémino/enzimologíaAsunto(s)
Fosfatasa Ácida/metabolismo , Encéfalo/enzimología , Quelantes/farmacología , Compuestos de Metilmercurio/farmacología , Succímero/farmacología , Compuestos de Sulfhidrilo/farmacología , Tiofenos/farmacología , Animales , Encéfalo/efectos de los fármacos , Cinética , Masculino , Compuestos de Metilmercurio/antagonistas & inhibidores , Especificidad de Órganos , Ratas , Ratas Endogámicas , Valores de Referencia , Factores de TiempoRESUMEN
The effect of methylmercury chloride (MMC) toxicity and its antagonism by chelating agents (N-acetyl-DL-homocysteine thiolactone and 2,3-dimercaptosuccinic acid) on succinic dehydrogenase (SDH) activity of fore-, mid-, and hindbrain and trigeminal ganglia of rats is reported in this study. A dose of 10 mg MMC/kg body weight was injected subcutaneously for 2, 7, and 15 days. The chelating agents were also injected subcutaneously in two separate groups of MMC-treated animals except in group 3. In the latter case MMC was injected in two groups of rats for 7 days, and thereafter antagonists were administered daily (40 mg/kg) for 1 week. The result shows inhibition of the enzyme with MMC in all groups and its restoration by N-acetyl-DL-homocysteine thiolactone; 2,3-dimercaptosuccinic acid, however, further reduced the enzyme level. The significance of inhibition of the enzyme in relation to tissue respiration and ATP production is discussed and the capacities of antagonists in the restoration of the SDH level are also analyzed.
Asunto(s)
Encéfalo/enzimología , Quelantes/farmacología , Reactivadores Enzimáticos , Ganglios Espinales/enzimología , Compuestos de Metilmercurio/farmacología , Succinato Deshidrogenasa/antagonistas & inhibidores , Animales , Masculino , Compuestos de Metilmercurio/antagonistas & inhibidores , Ratas , Succímero/farmacología , Succinato Deshidrogenasa/metabolismo , Tiofenos/farmacologíaAsunto(s)
Adenosina Trifosfatasas/antagonistas & inhibidores , Encéfalo/efectos de los fármacos , Compuestos de Metilmercurio/toxicidad , Animales , Encéfalo/enzimología , ATPasa de Ca(2+) y Mg(2+)/antagonistas & inhibidores , Quelantes/farmacología , Masculino , Compuestos de Metilmercurio/antagonistas & inhibidores , Ratas , Ratas Endogámicas , ATPasa Intercambiadora de Sodio-Potasio/antagonistas & inhibidoresRESUMEN
The study deals with alkaline phosphatase (ALK) changes in methylmercury chloride (MMC) and the antagonists, N-acetyl-DL-homocysteine thiolactone (NAHT) and 2,3-dimercaptosuccinic acid (DSA) treated rats. A daily dose of 10 mg/kg of MMC was given to the animals for two days, seven days, and fifteen days. The animals were sacrificed on third day, eighth day, fifteen day (kept for one week without treatment) and sixteenth day. The antagonists (40 mg/kg body weight) were also applied simultaneously except in third group animals where these agents were administered from 8th-14th days. Study reveals progressive decreased activity of the enzyme in all the animal groups with increasing the duration of the dose except two days treated animals. NAHT restored the enzyme level in all the groups except in fifteen days MMC treated animals, while DSA was less effective in all the groups except in two days MMC treated animals. The significance of inhibition of the enzyme in relation to methylmercury toxication has been discussed in detail.