Warming and drought weaken the carbon sink capacity of an endangered paleoendemic temperate rainforest in South America.

Measurements of ecosystem carbon (C) fluxes in temperate forests are concentrated in the Northern Hemisphere, leaving the functionally diverse temperate forests in the Southern Hemisphere underrepresented. Here, we report three years (February 2018-January 2021) of C fluxes, studied with eddy-covariance and closed chamber techniques, in an endangered temperate evergreen rainforest of the long-lived paleoendemic South American conifer Fitzroya cupressoides. Using classification and regression trees we analyzed the most relevant drivers and thresholds of daily net ecosystem exchange (NEE) and soil respiration. The annual NEE showed that the forest was a moderate C sink during the period analyzed (-287±38 g C m-2 year -1). We found that the capacity to capture C of the Fitzroya rainforests in the Coastal Range of southern Chile is optimal under cool and rainy conditions in the early austral spring (October-November) and decreases rapidly towards the summer dry season (January-February) and autumn. Although the studied forest type has a narrow geographical coverage, the gross primary productivity measured at the tower was highly representative of Fitzroya and other rainforests in the region. Our results suggest that C fluxes in paleoendemic cool F. cupressoides forests may be negatively affected by the warming and drying predicted by climate change models, reinforcing the importance of maintaining this and other long-term ecological research sites in the Southern Hemisphere.

Ecophysiological responses of Nothofagus obliqua forests to recent climate drying across the Mediterranean-Temperate biome transition in south-central Chile.

The forests of south-central Chile are facing a drying climate and a megadrought that started in 2010. This study addressed the physiological responses of five Nothofagus obliqua stands across the Mediterranean-Temperate gradient (35.9 ° -40.3° S) using carbon isotope discrimination (Δ13 C) and intrinsic water use efficiency (iWUE) in tree rings during 1967-2017. Moreover, δ18O was evaluated in the northernmost site to better understand the effects of the megadrought in this drier location. These forests have become more efficient in their use of water. However, trees from the densest stand are discriminating more against 13C, probably due to reduced photosynthetic rates associated with increasing competition. The strongest associations between climate and Δ13C were found in the northernmost stand, suggesting that warmer and drier conditions could have reduced 13C discrimination. Tree growth in this site has not decreased, and δ18O was negatively related to annual rainfall. However, a shift in this relationship was found since 2007, when both precipitation and δ18O decreased, while correlations between δ18O and growth increased. This implies that tree growth and δ18O are coupled in recent years, but precipitation is not the cause, suggesting that trees probably changed their water source to deeper and more depleted pools. Our research demonstrates that forests are not reducing their growth in central Chile, mainly due to a shift towards the use of deeper water sources. Despite a common climate trend across the gradient, there is a non-uniform response of N. obliqua forests to climate drying, being their response site specific. Keywords: Tree rings, stable isotopes, tree physiology, climate gradient, megadrought, climate change.

Low Growth Sensitivity and Fast Replenishment of Non-structural Carbohydrates in a Long-Lived Endangered Conifer After Drought.

There is an ongoing debate on whether a drought induced carbohydrate limitation (source limitation) or a direct effect of water shortage (sink limitation) limit growth under drought. In this study, we investigated the effects of the two driest summers recorded in southern Chile in the last seven decades, on the growth and non-structural carbohydrates (NSC) concentrations of the slow-growing conifer Fitzroya cupressoides. Specifically, we studied the seasonal variation of NSC in saplings and adults one and two years after the occurrence of a 2 year-summer drought at two sites of contrasting precipitation and productivity (mesic-productive vs. rainy-less productive). We also evaluated radial growth before, during and after the drought, and predicted that drought could have reduced growth. If drought caused C source limitation, we expected that NSCs will be lower during the first than the second year after drought. Conversely, similar NSC concentrations between years or higher NSC concentrations in the first year would be supportive of sink limitation. Also, due to the lower biomass of saplings compared with adults, we expected that saplings should experience stronger seasonal NSC remobilization than adults. We confirmed this last expectation. Moreover, we found no significant growth reduction during drought in the rainy site and a slightly significant growth reduction at the mesic site for both saplings and adults. Across organs and in both sites and age classes, NSC, starch, and sugar concentrations were generally higher in the first than in the second year following drought, while NSC seasonal remobilization was generally lower. Higher NSC concentrations along with lower seasonal NSC remobilization during the first post-drought year are supportive of sink limitation. However, as these results were found at both sites while growth decreased slightly and just at the mesic site, limited growth only is unlikely to have caused NSC accumulation. Rather, these results suggest that the post-drought dynamics of carbohydrate storage are partly decoupled from the growth dynamics, and that the rebuild of C reserves after drought may be a priority in this species.

Six hundred years of South American tree rings reveal an increase in severe hydroclimatic events since mid-20th century.

South American (SA) societies are highly vulnerable to droughts and pluvials, but lack of long-term climate observations severely limits our understanding of the global processes driving climatic variability in the region. The number and quality of SA climate-sensitive tree ring chronologies have significantly increased in recent decades, now providing a robust network of 286 records for characterizing hydroclimate variability since 1400 CE. We combine this network with a self-calibrated Palmer Drought Severity Index (scPDSI) dataset to derive the South American Drought Atlas (SADA) over the continent south of 12°S. The gridded annual reconstruction of austral summer scPDSI is the most spatially complete estimate of SA hydroclimate to date, and well matches past historical dry/wet events. Relating the SADA to the Australia-New Zealand Drought Atlas, sea surface temperatures and atmospheric pressure fields, we determine that the El Niño-Southern Oscillation (ENSO) and the Southern Annular Mode (SAM) are strongly associated with spatially extended droughts and pluvials over the SADA domain during the past several centuries. SADA also exhibits more extended severe droughts and extreme pluvials since the mid-20th century. Extensive droughts are consistent with the observed 20th-century trend toward positive SAM anomalies concomitant with the weakening of midlatitude Westerlies, while low-level moisture transport intensified by global warming has favored extreme rainfall across the subtropics. The SADA thus provides a long-term context for observed hydroclimatic changes and for 21st-century Intergovernmental Panel on Climate Change (IPCC) projections that suggest SA will experience more frequent/severe droughts and rainfall events as a consequence of increasing greenhouse gas emissions.

The Oldest, Slowest Rainforests in the World? Massive Biomass and Slow Carbon Dynamics of Fitzroya cupressoides Temperate Forests in Southern Chile.

Old-growth temperate rainforests are, per unit area, the largest and most long-lived stores of carbon in the terrestrial biosphere, but their carbon dynamics have rarely been described. The endangered Fitzroya cupressoides forests of southern South America include stands that are probably the oldest dense forest stands in the world, with long-lived trees and high standing biomass. We assess and compare aboveground biomass, and provide the first estimates of net primary productivity (NPP), carbon allocation and mean wood residence time in medium-age stands in the Alerce Costero National Park (AC) in the Coastal Range and in old-growth forests in the Alerce Andino National Park (AA) in the Andean Cordillera. Aboveground live biomass was 113-114 Mg C ha(-1) and 448-517 Mg C ha(-1) in AC and AA, respectively. Aboveground productivity was 3.35-3.36 Mg C ha(-1) year(-1) in AC and 2.22-2.54 Mg C ha(-1) year(-1) in AA, values generally lower than others reported for temperate wet forests worldwide, mainly due to the low woody growth of Fitzroya. NPP was 4.21-4.24 and 3.78-4.10 Mg C ha(-1) year(-1) in AC and AA, respectively. Estimated mean wood residence time was a minimum of 539-640 years for the whole forest in the Andes and 1368-1393 years for only Fitzroya in this site. Our biomass estimates for the Andes place these ecosystems among the most massive forests in the world. Differences in biomass production between sites seem mostly apparent as differences in allocation rather than productivity. Residence time estimates for Fitzroya are the highest reported for any species and carbon dynamics in these forests are the slowest reported for wet forests worldwide. Although primary productivity is low in Fitzroya forests, they probably act as ongoing biomass carbon sinks on long-term timescales due to their low mortality rates and exceptionally long residence times that allow biomass to be accumulated for millennia.