Tocotrienols reach the brain and play roles in the attenuation of body weight gain and improvement of cognitive function in high-fat diet-treated mice.

Obesity induces severe disorders such as type 2 diabetes and cardiovascular events, and the number of people with obesity is increasing all over the world. Furthermore, it is possible that obesity increases the risk of cognitive dysfunction via the acceleration of oxidative damage. Tocotrienols, which are part of the vitamin E family, have antioxidant and anti-obesity effects. However, the effects of tocotrienols on high-fat diet-treated mice have not been completely elucidated. In this study, we assessed changes in body weight, spatial reference memory acquisition, liver lipid droplet size, blood brain barrier-related protein expressions and antioxidative defense systems in high-fat diet-treated mice in the presence or absence of tocotrienols. The results showed that tocotrienols significantly inhibited body weight gain and lipid droplet synthesis. Although the amount was very small, it was confirmed that tocotrienols surely reached the brain in the perfused brain. Treatment with tocotrienols was tended to improve cognitive function in the control mice. However, tocotrienols did not modulate blood brain barrier-related protein expressions or antioxidative defense systems. These results indicate that treatment with tocotrienols could be effective for the prevention of obesity and cognitive dysfunction. Further extended research is needed to elucidate the relationship between anti-obesity and antioxidant effects of tocotrienols, especially in the brain.

Risk factors for cancellation after dispatch of rapid response cars for prehospital emergency care: a single-center, case-control study.

AIM: The objective of this study is to identify the risk factors for cancellation after dispatch of rapid response cars (RRC) for prehospital emergency care. METHODS: We retrospectively extracted data from all RRC cases dispatched from our hospital between April 2017 and March 2019. A total of 1,440 cases were included in our study and divided into either the "cancelled" group (n = 723) or the "treated" group (n = 717), based on the occurrence of cancellation. The variables obtained from the request calls for RRC included patient characteristics, distance from the hospital to the scene, and reasons for RRC request. The variables were compared between the two groups and logistic regression analysis was carried out to identify the risk factors for RRC cancellation. RESULTS: Multivariable analysis showed that distance from the hospital to the scene (odds ratio [OR] 1.25; 95% confidence interval (CI), 1.21-1.28), suspicion of cardiopulmonary arrest with no witness information (OR 7.61; 95% CI, 4.13-14.00), dyspnea (OR 2.22; 95% CI, 1.19-4.11), and suicide by hanging (OR 3.49; 95% CI, 1.37-8.89) were independent risk factors for cancellation. CONCLUSIONS: In our study, a greater distance from the hospital to the scene, suspicion of cardiopulmonary arrest with no witness information, dyspnea, and suicide by hanging were identified as independent risk factors for cancellation after dispatch of RRC. Evaluating the risk factors for cancellation at individual facilities could help hospitals adjust their dispatch criteria to allocate limited medical resources more effectively.

Improvement of Bio-Oil and Nitrogen Recovery from Microalgae Using Two-Stage Hydrothermal Liquefaction with Solid Carbon and HCl Acid Catalysis.

Bio-oil production from microalgae by using hydrothermal liquefaction (HTL) has been conducted extensively in the last decade. In this work, we conducted two-stage HTL of a microalga (Fistulifera solaris, JPCC DA0580) in the presence of 5.0 g/L carbon solid acid or a 0.02-0.50 M HCl catalyst to increase bio-oil yield and nitrogen recovery into the aqueous phase (AP). The first stage (HTL 1), to hydrolyze proteins, carbohydrates, and lipids and elute nitrogen components into the AP, was conducted at 100-250 °C for 30-120 min. The second stage (HTL 2), to produce the bio-oil, was conducted at 280-320 °C for 0-30 min. The best conditions to obtain a high bio-oil yield and NH4 + recovery in the AP were 200 °C and 30 min of residence time for HTL 1 and 320 °C and 0 min residence time for HTL 2. We found that 0.50 M HCl decreased the bio-oil yield while greatly increasing NH4 + in the AP and decreasing the nitrogen content in the bio-oil. This was probably due to the catalytic effect of HCl promoting hydrolysis of protein and deamination of amino acids during HTL 1. The fractions of water-soluble products were greatly increased by performing HTL 2 in neutral conditions while this maintained low nitrogen content in the bio-oil. From GC-MS analyses of the bio-oil, it was observed that, by using 0.50 M HCl, peak intensities of all the GC peaks decreased and MS spectra of amines decreased. The carbon solid acid had an insignificant influence on bio-oil and NH4 + yields.

Fabrication of two-dimensional close-packed shell structure in ceramic thin films.

TiO2 thin films with a periodical two-dimensional close-packed hemispherical structure were prepared on Si substrates using pulsed laser deposition and close-packed monolayer polystyrene colloidal crystals as a template. Compared with conventional methods, which use a top-down approach, this route supports low-cost production of a periodic structure. Additionally, it is applicable to various ceramics for use in applications related to photonic crystals, surface self-cleaning materials, data storage media, bioassays, and so on.