RESUMEN
The influence of a vaccine based on the MB-7 strain of hepatitis A virus (VP-MB-7) designed at the "Vector" Center of Virology and Biotechnology was studied. VP-MB-7 was found to provoke no allergic response and to have an activating effect on the specific and non-specific responses of cell and humoral immunity similar to those evoked by hepatitis A vaccine "Hep-A-in Vac".
Asunto(s)
Anticuerpos de Hepatitis A/biosíntesis , Vacunas contra la Hepatitis A/administración & dosificación , Vacunas contra la Hepatitis A/inmunología , Virus de la Hepatitis A Humana/inmunología , Hepatitis A/prevención & control , Vacunación , Animales , Recuento de Células , Evaluación Preclínica de Medicamentos , Cobayas , Hepatitis A/sangre , Inmunidad Celular , Recuento de Linfocitos , Ratones , Ratones Endogámicos BALB C , Bazo/inmunología , Subgrupos de Linfocitos T/inmunología , Linfocitos T Colaboradores-Inductores/inmunologíaRESUMEN
A study was undertaken to prove that a vaccine drug based on the fast-growing MB-7 (MB-7 VD) of hepatitis A virus (HAV) is safe. It was established that MB-7 VD, when once injected to white outbred mice and when 4 times injected to Hartley guineas pigs, did not cause any hematological and biochemical changes in the peripheral blood or in the condition of the central nervous system of experimental animals, which shows that MB-7 VD is free of any toxic properties.
Asunto(s)
Vacunas contra la Hepatitis A/administración & dosificación , Hepatitis A/prevención & control , Vacunación , Animales , Recuento de Células Sanguíneas , Evaluación Preclínica de Medicamentos , Eritrocitos/inmunología , Cobayas , Hemoglobinas/análisis , Hepatitis A/sangre , Hepatitis A/inmunología , Vacunas contra la Hepatitis A/inmunología , Linfocitos/inmunología , RatonesRESUMEN
Measles predominates among childhood droplet infections in many countries. Immunization of all human beings sensitive to this infection is the only radical measure in controlling measles. The quality of a vaccine is primarily determined by the properties of the virus strains and cell cultures and technology of production. Now live measles vaccine is produced in or country on the basis of fibroblasts from Japanese quail embryo. The production of live measles vaccine in the primary cell cultures has a number of drawbacks caused by the nonstandard pattern of the substrate and the probability of contamination. The use the certified human diploid cells deposited in liquid nitrogen in sufficient quantities is promising. The authors have elaborated a new technology of live measles vaccine production by using the Leningrad-16 virus strain on the basis of attested L-68 diploid cell culture from the human fetal lung. Experimental batches of vaccine were obtained and attested in accordance with the present requirements for immunobiological products.
Asunto(s)
Embrión de Mamíferos/virología , Pulmón , Vacuna Antisarampión/biosíntesis , Sarampión/prevención & control , Animales , Anticuerpos Antivirales/análisis , Células Cultivadas/virología , Embrión de Mamíferos/citología , Embrión de Mamíferos/efectos de los fármacos , Cobayas , Humanos , Sarampión/inmunología , Sarampión/virología , Vacuna Antisarampión/uso terapéutico , Virus del Sarampión/crecimiento & desarrollo , Virus del Sarampión/inmunología , Virus del Sarampión/patogenicidadRESUMEN
In the past 5 years, the investigators of the "VECTOR" SRB VB and the L.A. Tarasevich State Institute of Standardization and Control of Medical Biological Preparations have jointly designed sera reference panels containing anti-HIV-1 IgG, anti-HCV IgG, and anti-HAV IgM which have been approved as national standard panels. The panels are intended for use in controlling the specificity and stability of the most widely used ELISA diagnostic kits and immunoblot test systems during production, control, and application stages. Some problems of development and production of these panels, including the representation of different sera in the panels and the selection of specific IgG concentrations in the different sera in the panel are described. The authors also attract attention to the stabilization of the specific characteristics of panel sera during storage and transportation.
Asunto(s)
Ensayo de Inmunoadsorción Enzimática/normas , VIH-1/inmunología , Hepacivirus/inmunología , Hepatovirus/inmunología , Juego de Reactivos para Diagnóstico/normas , Anticuerpos Anti-VIH/análisis , Anticuerpos contra la Hepatitis B/análisis , Anticuerpos contra la Hepatitis C/análisis , Humanos , Inmunoglobulina G/análisis , Inmunoglobulina M/análisis , Control de Calidad , Estándares de Referencia , Federación de RusiaAsunto(s)
Antineoplásicos/farmacología , Factor de Necrosis Tumoral alfa/farmacología , Sustitución de Aminoácidos , Animales , Antineoplásicos/farmacocinética , Arginina/genética , Ensayos de Selección de Medicamentos Antitumorales , Estabilidad de Medicamentos , Humanos , Neoplasias Laríngeas/patología , Leucemia Monocítica Aguda/patología , Ratones , Conejos , Proteínas Recombinantes/genética , Proteínas Recombinantes/farmacocinética , Proteínas Recombinantes/farmacología , Células Tumorales Cultivadas , Factor de Necrosis Tumoral alfa/genética , Factor de Necrosis Tumoral alfa/farmacocinéticaAsunto(s)
Anticuerpos Anti-VIH/sangre , Sueros Inmunes , Técnicas para Inmunoenzimas/normas , Juego de Reactivos para Diagnóstico/normas , Intervalos de Confianza , Humanos , Técnicas para Inmunoenzimas/instrumentación , Técnicas para Inmunoenzimas/estadística & datos numéricos , Distribución Normal , Control de Calidad , Juego de Reactivos para Diagnóstico/estadística & datos numéricos , Sensibilidad y Especificidad , U.R.S.S.RESUMEN
Materials on the study of relationship between the activity of immunoreactive insulin and the degree of an increase in the level of anti-influenza antibodies, induced by vaccine strains, are presented. A short-term decrease in the activity of insulin, more pronounced in donors immunized with adsorbed chemical influenza vaccine, and an increase in the antibody level, mainly in the group of donors receiving killed influenza vaccine, have been noted.
Asunto(s)
Donantes de Sangre , Vacunas contra la Influenza/inmunología , Insulina/sangre , Humanos , Inmunización , Anticuerpos Insulínicos/análisis , Factores de Tiempo , Vacunas Atenuadas/inmunologíaRESUMEN
The article discusses the 10-year experience in the use of radioisotop diagnosis in the practice of an intensive therapy department. The results of the examination of over 300 patients who had been operated on under conditions of extracorporeal circulation are analyzed. With the use of radioactive indicators some new aspects of the pathophysiology of acute circulatory disorders were revealed, among others those related to the problem of blood deposition, the correlation between the functioning of the right and left parts of the heart; the diagnostic possibilities in appraising the causes of the disorders of circulation, functioning of the parenchymatous organs, and tissue blood flow were widened; the therapeutic tactics in the development of hypovolemia, acute cardiac failure, and disturbances in tissue perfusion could be elaborated.