Genotoxic effects of tobacco use in residents of hilly areas and foot hills of Western Ghats, Southern India.

Smoking and smokeless tobacco consumption is a significant risk factor that provokes genetic alterations. The present investigation was to evaluate the biomarkers of genotoxicity including micronucleus (MN), chromosome aberrations (CA) and DNA strand breaks among tobacco consumers and control individuals residing in hilly areas of Western Ghats, Tamilnadu, South India. This study included 268 tobacco consumers with equal number of controls. The tobacco consumers were divided into Group I (<10 years of tobacco consumption with an age range from 15 to 35 years) and group II (>10 years consumption above 35 years of age). Chromosome aberration (CA) and comet assay were performed using blood and micronucleus assay from exfoliated buccal epithelial cells obtained from tobacco consumers and controls. Elevated levels of CA were found in group II (Chromatid type: 2.39 ± 1.13 and chromosome type: 1.44 ± 1.24) exposed subjects, high micronucleus and DNA damage (TL:4.48 ± 1.24 and TM:3.40 ± 1.58) levels were significantly (p < 0.05) observed in both smoking and smokeless tobacco consumers when comparison with group I and controls. This study also observed a lack of awareness among the tobacco consumers about the harmful health effects of tobacco. Tobacco consumption contributes to the significant alteration in genetic materials. In addition, a high rate of spontaneous abortion was also seen in the studied population.

Utilization of marine fisheries wastes for the production of the freshwater fish Cyprinus carpio.

The present study was aimed to assess the effects of complete replacement of fish meal with fisheries waste meals on survival, growth performance, digestive enzyme activities, and muscle compositions of the freshwater fish Cyprinus carpio. The proximate composition and mineral contents of three different fisheries wastes, such as Rastrelliger kanagurta, Sphyraena barracuda, and Fenneropenaeus indicus were analyzed. Based on the nutrient content of these fisheries waste, one control fish meal diet and three different complete fish meal replacement diets (diet 1, diet 2, and diet 3 formulated with R. kanagurta, S. barracuda, and F. indicus waste meals, respectively) were formulated. Fingerlings C. carpio were fed with these diets for a period of 6 weeks. Results from feeding experiments showed insignificant (p > 0.05) differences in survival, growth, and feed intake of C. carpio fed with control and three different fisheries waste diets. However, the digestive enzyme activity and muscle biochemical compositions were significantly (p < 0.05) altered in F. indicus waste meal fed C. carpio compared to other fisheries waste meal and control diets fed fish groups. Therefore, the present study suggests that R. kanagurta, S. barracuda, and F. indicus waste meals can be considered as alternative feed ingredients for fish meal to formulate low-cost feeds for C. carpio culture.

Evaluation of clinical and cytogenetic parameters in rheumatoid arthritis patients for effective diagnosis.

BACKGROUND: Rheumatoid arthritis is the commonest inflammatory joint disease, affecting nearly 1% of the adult population worldwide. Early and accurate diagnosis and prognosis of rheumatoid arthritis (RA) have become increasingly important. In the present study, we aimed to elucidate the relationships between hematological, biochemical, immunological and cytogenetic parameters in rheumatoid arthritis patients and healthy normal controls. METHODS: The study group comprised of 126 RA patients and equal number of healthy normal control subjects. The blood was collected and analyzed for biochemical, immunological, enzymatic and cytogenetic parameters. RESULTS: Results of the present study indicated that 20% of RA patient's hematological, 31% of biochemical and 70% immunological parameters had a significant difference from the controls and reference range. The RF and anti-CCP antibody levels were also positive in 70% of RA patients. A significant increase in minor chromosomal abnormalities was also observed in patients as compared to controls. CONCLUSION: The knowledge about autoimmune diseases is very low among the South Indian population. The present study has thus helped in understanding the RA disease in a better way based on a pattern of various clinical markers of the disease condition which might help in planning therapeutic intervention strategies and create awareness about the disease management among RA patients of the population studied.