RESUMEN
Classical monoamines are well-known modulators of sensorimotor neural networks. However, the role of trace amines and their receptors in sensorimotor function remains unexplored. Using trace amine-associated receptor 5 knockout (TAAR5-KO) mice, that express beta-galactosidase mapping its localization, we observed TAAR5 expression in the Purkinje cells of the cerebellum and the medial vestibular nucleus, suggesting that TAAR5 might be involved in the vestibular and motor control. Accordingly, in various behavioral tests, TAAR5-KO mice demonstrated lower endurance, but better coordination and balance compared to wild-type controls. Furthermore, we found specific changes in striatal local field potentials and motor cortex electrocorticogram, such as a decrease in delta and an increase in theta oscillations of power spectra, respectively. The obtained data indicate that TAAR5 plays a considerable role in regulation postural stability, muscle force, balance, and motor coordination during active movements, likely via modulation of monoaminergic systems at different levels of sensorimotor control involving critical brain areas such as the brainstem, cerebellum, and forebrain.
Asunto(s)
Destreza Motora , Receptores Acoplados a Proteínas G/genética , Receptores Acoplados a Proteínas G/fisiología , Corteza Sensoriomotora/fisiología , Animales , Conducta Animal , Tronco Encefálico , Cerebelo/metabolismo , Electrofisiología , Perfilación de la Expresión Génica , Regulación de la Expresión Génica , Masculino , Ratones , Ratones Noqueados , Neurofisiología , Fenotipo , Corteza Sensoriomotora/metabolismo , Núcleos Vestibulares/metabolismoRESUMEN
AIM: Development of primers for the detection of leptospirae in clinical material including urine. MATERIALS AND METHODS: Study of specificity and sensitivity of primers complementary to colA gene in standard PCR by using DNA preparation of cultures of pathogenic and saprophytic leptospirae, biological materials from healthy humans and dogs, including contaminated with pathogenic leptospirae culture. RESULTS: Specific interaction of these primers with DNA of pathogenic leptospirae of 14 serogroups was established. Sensitivity of the technique was 50 cells in 1 ml of sample. CONCLUSION: The primers described fulfill the requirements for the sensitivity and specificity and can be recommended for the detection of leptospirae in both serum and urine.
Asunto(s)
Colagenasas/genética , Leptospira/aislamiento & purificación , Leptospirosis/diagnóstico , Reacción en Cadena de la Polimerasa/métodos , Animales , Cartilla de ADN/genética , ADN Bacteriano/genética , ADN Bacteriano/aislamiento & purificación , Perros , Humanos , Leptospira/genética , Leptospirosis/sangre , Leptospirosis/orina , Sensibilidad y EspecificidadRESUMEN
We analyzed restriction fragment length polymorphism of lipL32 gene encoding outer membrane protein in three Leptospira genomic species (L. interrogans, L. kirschneri, and L. borgpetersenii) using Bsa29 I and Bam HI restriction endonucleases. It was found that restriction profiles of the studied gene were similar at both the intraspecific and serogroup levels; variability was revealed only at the interspecific level, which can be explained by relatively low variability of lipL32 gene.
