Comparative rhythmic transcriptome profiling of human and mouse striatal subregions.

The human striatum can be subdivided into the caudate, putamen, and nucleus accumbens (NAc). In mice, this roughly corresponds to the dorsal medial striatum (DMS), dorsal lateral striatum (DLS), and ventral striatum (NAc). Each of these structures have some overlapping and distinct functions related to motor control, cognitive processing, motivation, and reward. Previously, we used a "time-of-death" approach to identify diurnal rhythms in RNA transcripts in these three human striatal subregions. Here, we identify molecular rhythms across similar striatal subregions collected from C57BL/6J mice across 6 times of day and compare results to the human striatum. Pathway analysis indicates a large degree of overlap between species in rhythmic transcripts involved in processes like cellular stress, energy metabolism, and translation. Notably, a striking finding in humans is that small nucleolar RNAs (snoRNAs) and long non-coding RNAs (lncRNAs) are among the most highly rhythmic transcripts in the NAc and this is not conserved in mice, suggesting the rhythmicity of RNA processing in this region could be uniquely human. Furthermore, the peak timing of overlapping rhythmic genes is altered between species, but not consistently in one direction. Taken together, these studies reveal conserved as well as distinct transcriptome rhythms across the human and mouse striatum and are an important step in understanding the normal function of diurnal rhythms in humans and model organisms in these regions and how disruption could lead to pathology.

Diurnal Alterations in Gene Expression Across Striatal Subregions in Psychosis.

BACKGROUND: Psychosis is a defining feature of schizophrenia and highly prevalent in bipolar disorder. Notably, individuals with these illnesses also have major disruptions in sleep and circadian rhythms, and disturbances of sleep and circadian rhythms can precipitate or exacerbate psychotic symptoms. Psychosis is associated with the striatum, though to our knowledge, no study to date has directly measured molecular rhythms and determined how they are altered in the striatum of subjects with psychosis. METHODS: We performed RNA sequencing and both differential expression and rhythmicity analyses to investigate diurnal alterations in gene expression in human postmortem striatal subregions (nucleus accumbens, caudate, and putamen) in subjects with psychosis (n = 36) relative to unaffected comparison subjects (n = 36). RESULTS: Across regions, we found differential expression of immune-related transcripts and a substantial loss of rhythmicity in core circadian clock genes in subjects with psychosis. In the nucleus accumbens, mitochondrial-related transcripts had decreased expression in subjects with psychosis, but only in those who died at night. Additionally, we found a loss of rhythmicity in small nucleolar RNAs and a gain of rhythmicity in glutamatergic signaling in the nucleus accumbens of subjects with psychosis. Between-region comparisons indicated that rhythmicity in the caudate and putamen was far more similar in subjects with psychosis than in matched comparison subjects. CONCLUSIONS: Together, these findings reveal differential and rhythmic gene expression differences across the striatum that may contribute to striatal dysfunction and psychosis in psychotic disorders.

Heterostructured γ-Fe2O3/FeTiO3 magnetic nanocomposite: An efficient visible-light-driven photocatalyst for the degradation of organic dye.

The catalyst recovery is the major concern in commercialization of photocatalysts for the industrial effluent treatment process. To overcome this major issue, Fe2O3 based magnetic photocatalytic heterostructure É£-Fe2O3/FeTiO3 nanocomposite was synthesized by hydrothermal method. Fe2O3 is the cheapest visible active magnetic photocatalytic material, but it has the limitation of fast e-/h + recombination. Titanium (Ti) was loaded on γ-Fe2O3 to overcome this issue. The loaded Ti has grown as FeTiO3 on the surface of É£-Fe2O3 nanocrystals and emerged as heterostructure É£- Fe2O3/FeTiO3 nanocomposites, which was confirmed by XRD and TEM results. The loading concentration of Ti on γ-Fe2O3 was optimized to achieve the maximum photocatalytic efficiency without compromising the magnetic property of γ-Fe2O3 to facilitate the magnetic separation. DRS-UV spectra revealed the strong visible light response of γ- Fe2O3/FeTiO3 nanocomposite. The photocatalytic efficiencies of the synthesized materials were evaluated using methylene blue (MB) as a model pollutant under sunlight. The built-in electric field between p-n junction between FeTiO3 and Fe2O3 and type II charge transfer mechanism extended the lifetime of the charge carriers at the heterojunction of γ- Fe2O3/FeTiO3, which was confirmed by PL spectra. The vibrating sample magnetometer (VSM) study revealed the decreasing magnetization, coercivity (Hc), and retentivity (Mr) of γ-Fe2O3 with increasing concentration of Ti. 92% of the used-up 20 wt% Ti loaded γ-Fe2O3/FeTiO3 magnetic nanocomposite was recovered from the treated wastewater using an electromagnet. Both magnetic properties and efficiency of the nanocomposite increased up to 20 wt% of Ti loading, beyond that decreased due to the increasing composition of antiferromagnetic FeTiO3 and the increasing number of defect sites as recombination centers. Hence, 20 wt% loading of Ti was concluded as the optimum to enhance the efficiency and to retain the magnetic properties. This work aims the commercialization of magnetic photocatalytic materials for the industrial effluent treatment.

Synthesis of tri-metallic surface engineered nanobiochar from cynodon dactylon residues in a single step - Batch and column studies for the removal of copper and lead ions.

Superparamagnetic nanocomposites integrated with multiple metals, and surface engineered nanoparticles play a vital role in the removal of heavy metals. In the present study, amino-functional silica-coated magnetic nanocomposites with biochar synthesised from Cynodon dactylon plant residues are prepared in a single step reaction process. The synthesised nanocomposites are characterized using various analytical techniques such as FTIR to determine their functional entities, SEM, TEM, EDX and VSM to analyse the size (~50 nm), elements and magnetic nature of the nanocomposites. Characterization reveals that the prepared nanobiochar was coated with silica and a specific amine group. The magnetic saturation value of 50 emu/g confirms the prepared sorbent was superparamagnetic. Kinetics, isotherm and thermodynamics parameters are evaluated to study the metal interaction mechanism with the nanocomposites where the system follows pseudo-second-order kinetics and the four-parameter Fritz Schlunder model for both metal ions. The nanocomposites showed the enhanced adsorption capacity of copper (Cu(II)) ions with 220.4 mg/g and 185.4 mg/g for lead (Pb(II)) ions. The nanocomposites also showed the excessive reusing ability of 15 times with the maximum removal efficiency for Cu(II) and Pb(II) metal ions. Column studies are evaluated to demonstrate the vital performance in the removal of Cu(II) ions and the breakthrough point was inferred for the parameters such as concentration (100-300 mg/L), bed height (1-3 cm) and flow rate (2-4 mL/min). The breakthrough point was attained at 1400 min and the removal efficiency of about 64.58% was obtained.

Tooth shade variation in Indian population: An objective guide to age estimation.

INTRODUCTION: The color of the teeth is affected by chronological age due to the variations of the hard and soft tissue structure of the teeth. There are very few studies have assessed the shade of the Enamel and correlated it with the age changes. Hence the study aimed to assess the enamel shade with the aging in the individual. MATERIALS AND METHODS: The central incisors of 388 individuals (5-78 years) were assessed for tooth color using a VITA 3D master shade guide and the Hue, Value and Chroma were noted. Mean grey values of the teeth were obtained by image manipulation using Image J software and normalized using the values obtained from the 18% contrast grey card. Chi square tests and linear regression analysis is used to find associations with age and tooth shade variations.Results: The teeth had higher score of Hue, Value and Chroma in older individuals. The tooth shade tended to shift towards redder hue with older age group. Simple linear regression analysis showed a significant correlation of age with normalized grey value in association with the shade parameters. (r = 0.717, SEE = 12.322 years)Conclusion: Tooth color changes with age and the mean age in grey values and shade guides can be a useful tool for age estimation.

Diurnal rhythms across the human dorsal and ventral striatum.

The human striatum can be subdivided into the caudate, putamen, and nucleus accumbens (NAc). Each of these structures have some overlapping and some distinct functions related to motor control, cognitive processing, motivation, and reward. Previously, we used a "time-of-death" approach to identify diurnal rhythms in RNA transcripts in human cortical regions. Here, we identify molecular rhythms across the three striatal subregions collected from postmortem human brain tissue in subjects without psychiatric or neurological disorders. Core circadian clock genes are rhythmic across all three regions and show strong phase concordance across regions. However, the putamen contains a much larger number of significantly rhythmic transcripts than the other two regions. Moreover, there are many differences in pathways that are rhythmic across regions. Strikingly, the top rhythmic transcripts in NAc (but not the other regions) are predominantly small nucleolar RNAs and long noncoding RNAs, suggesting that a completely different mechanism might be used for the regulation of diurnal rhythms in translation and/or RNA processing in the NAc versus the other regions. Further, although the NAc and putamen are generally in phase with regard to timing of expression rhythms, the NAc and caudate, and caudate and putamen, have several clusters of discordant rhythmic transcripts, suggesting a temporal wave of specific cellular processes across the striatum. Taken together, these studies reveal distinct transcriptome rhythms across the human striatum and are an important step in helping to understand the normal function of diurnal rhythms in these regions and how disruption could lead to pathology.