Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Más filtros










Base de datos
Intervalo de año de publicación
1.
Cytogenet Genome Res ; 142(1): 14-20, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-24192547

RESUMEN

The fate of cultivated primary hematopoietic stem cells (HSCs) with respect to genetic instability and telomere attrition has not yet been described in great detail. Thus, knowledge of the genetic constitution of HSCs is important when interpreting results of HSCs in culture. While establishing a cell culture model for myelodysplastic syndrome with a deletion in 5q by performing RPS14 knockdown, we found surprising data that may be of importance for any CD34+ cell culture experiments. We performed cytogenetic analyses and telomere length measurement on transduced CD34+ cells and untransduced control cells to observe the effects of long-term culturing. Initially, CD34+ cells had a normal median telomere length of about 12 kb and showed no signs of chromosomal instability. During follow-up, the median telomere length seemed to decrease and, simultaneously, increased chromosomal instability could be observed - in modified and control cells. One culture showed a clonal monosomy 7 - independent of prior RPS14 knockdown. During further culturing, it seemed that the telomeres re-elongated, and chromosomes stabilized, while TERT expression was not elevated. In summary, irrespective of our results of RPS14 knockdown in the long-term culture of CD34+ cells, it becomes clear that cell culture artefacts inducing telomere shortening and chromosomal instability have to be taken into account and regular cytogenetic analyses should always be performed.


Asunto(s)
Artefactos , Técnicas de Cultivo de Célula , Inestabilidad Cromosómica/genética , Cromosomas Humanos Par 5/genética , Células Madre Hematopoyéticas/ultraestructura , Proteínas Ribosómicas/genética , Acortamiento del Telómero/genética , Antígenos CD34/análisis , Células Cultivadas , Deleción Cromosómica , Cromosomas Humanos Par 5/ultraestructura , Ensayo de Unidades Formadoras de Colonias , Reparación del ADN , Sangre Fetal/citología , Genes Reporteros , Células Madre Hematopoyéticas/citología , Humanos , Hibridación Fluorescente in Situ , Células K562 , Cariotipificación , Síndromes Mielodisplásicos/genética , Síndromes Mielodisplásicos/patología , Reacción en Cadena de la Polimerasa , Interferencia de ARN , ARN Interferente Pequeño/genética , Proteínas Ribosómicas/biosíntesis , Proteínas Ribosómicas/deficiencia , Proteínas Ribosómicas/fisiología , Telomerasa/metabolismo , Transducción Genética
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA