Energy efficiency in winemaking industry: Challenges and opportunities.

The United Nations has issued a warning over the limited time for climate disaster prevention. In the last two decades, several countries have set targets to reduce fossil fuel usage and greenhouse gas emissions. These goals are tracked through the adoption of energy systems that prioritise efficiency and low-carbon alternatives, in alignment with the Sustainable Development Goals outlined by the United Nations. In the winemaking sector, the wine produced in the European Union comprised 65 % of the worldwide total from 2014 to 2018, with vineyards making up 4.7 % of its farms in 2020. Electricity is the primary source of energy used in vineries, accounting for around 90 % of the total energy consumption. The energy consumption associated with winemaking is mostly attributed to two key processes: fermentation, which accounts for 45 % to 90 % of the entire energy consumption, and bottling and storage, which contribute around 18 % of the overall energy consumption. The aim of this article is to provide an integrated review of energy efficiency in wineries through examining 144 academic publications. The selected publications cover various aspects, including sustainable energy utilisation in the wine industry, thermal performance analysis of buildings, energy efficiency assessment of systems and technologies, and the integration of renewable energy sources. A link has been established between the geographic distribution of academic publications and wine-producing countries. In relation to European publications, it is observed that research funding is associated with the energy directives of the European Union. It can also be concluded that wine customers are pushing for environmentally friendly practices. However, not everyone in the winemaking sector is moving in the same direction or at the same pace. To identify areas for improvement, winemakers must have supporting tools to manage energy use. Systems optimisation, monitoring, and accounting can be used to decrease energy consumption in winemaking processes or equipment. Progresses on sustainable energy use through greater energy efficiency and share of renewable energies in the wineries can contribute to the reduction of greenhouse gas emissions, and consequently, brings the wine industry closer to climate neutrality.

Incident Use of Hydroxychloroquine for the Treatment of Rheumatoid Arthritis and Systemic Lupus Erythematosus During the COVID-19 Pandemic.

OBJECTIVE: We studied whether the use of hydroxychloroquine (HCQ) for COVID-19 resulted in supply shortages for patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). METHODS: We used US claims data (IQVIA PHARMETRICS® Plus for Academics [PHARMETRICS]) and hospital electronic records from Spain (Institut Municipal d'Assistència Sanitària Information System [IMASIS]) to estimate monthly rates of HCQ use between January 2019 and March 2022, in the general population and in patients with RA and SLE. Methotrexate (MTX) use was estimated as a control. RESULTS: More than 13.5 million individuals (13,311,811 PHARMETRICS, 207,646 IMASIS) were included in the general population cohort. RA and SLE cohorts enrolled 135,259 and 39,295 patients, respectively, in PHARMETRICS. Incidence of MTX and HCQ were stable before March 2020. On March 2020, the incidence of HCQ increased by 9- and 67-fold in PHARMETRICS and IMASIS, respectively, and decreased in May 2020. Usage rates of HCQ went back to prepandemic trends in Spain but remained high in the United States, mimicking waves of COVID-19. No significant changes in HCQ use were noted among patients with RA and SLE. MTX use rates decreased during HCQ approval period for COVID-19 treatment. CONCLUSION: Use of HCQ increased dramatically in the general population in both Spain and the United States during March and April 2020. Whereas Spain returned to prepandemic rates after the first wave, use of HCQ remained high and followed waves of COVID-19 in the United States. However, we found no evidence of general shortages in the use of HCQ for both RA and SLE in the United States.

Human-Aware Collaborative Robots in the Wild: Coping with Uncertainty in Activity Recognition.

This study presents a novel approach to cope with the human behaviour uncertainty during Human-Robot Collaboration (HRC) in dynamic and unstructured environments, such as agriculture, forestry, and construction. These challenging tasks, which often require excessive time, labour and are hazardous for humans, provide ample room for improvement through collaboration with robots. However, the integration of humans in-the-loop raises open challenges due to the uncertainty that comes with the ambiguous nature of human behaviour. Such uncertainty makes it difficult to represent high-level human behaviour based on low-level sensory input data. The proposed Fuzzy State-Long Short-Term Memory (FS-LSTM) approach addresses this challenge by fuzzifying ambiguous sensory data and developing a combined activity recognition and sequence modelling system using state machines and the LSTM deep learning method. The evaluation process compares the traditional LSTM approach with raw sensory data inputs, a Fuzzy-LSTM approach with fuzzified inputs, and the proposed FS-LSTM approach. The results show that the use of fuzzified inputs significantly improves accuracy compared to traditional LSTM, and, while the fuzzy state machine approach provides similar results than the fuzzy one, it offers the added benefits of ensuring feasible transitions between activities with improved computational efficiency.

Novel short-term national strategies to promote the use of renewable hydrogen in road transport: A life cycle assessment of passenger car fleets partially fuelled with hydrogen.

This work presents an energy analysis combined with a comparative environmental life cycle assessment (LCA) of eight different passenger car fleets that use renewable hydrogen and a conventional fuel (natural gas or gasoline) under the same total energy input and the same hydrogen-to-mixture energy ratio. The fleets under comparison involve vehicles that use the two fuels separately or in a mixture. Using Italy as an illustrative country, this research work aims to help policy-makers implement well-supported strategies to promote the use of hydrogen in road transport in the short term. The proposed strategies achieve a carbon footprint reduction between 7 % and 35 % with respect to their conventional fleet benchmark. Within the current context, the results suggest the energy and environmental suitability of using hydrogen blends as short-term solutions, involving vehicles that require minor modifications with respect to current compressed natural gas vehicles and gasoline vehicles, while paving the way for pure hydrogen mobility.

A Fuzzy Inference System for the Assessment of Indoor Air Quality in an Operating Room to Prevent Surgical Site Infection.

Indoor air quality in hospital operating rooms is of great concern for the prevention of surgical site infections (SSI). A wide range of relevant medical and engineering literature has shown that the reduction in air contamination can be achieved by introducing a more efficient set of controls of HVAC systems and exploiting alarms and monitoring systems that allow having a clear report of the internal air status level. In this paper, an operating room air quality monitoring system based on a fuzzy decision support system has been proposed in order to help hospital staff responsible to guarantee a safe environment. The goal of the work is to reduce the airborne contamination in order to optimize the surgical environment, thus preventing the occurrence of SSI and reducing the related mortality rate. The advantage of FIS is that the evaluation of the air quality is based on easy-to-find input data established on the best combination of parameters and level of alert. Compared to other literature works, the proposed approach based on the FIS has been designed to take into account also the movement of clinicians in the operating room in order to monitor unauthorized paths. The test of the proposed strategy has been executed by exploiting data collected by ad-hoc sensors placed inside a real operating block during the experimental activities of the "Bacterial Infections Post Surgery" Project (BIPS). Results show that the system is capable to return risk values with extreme precision.

Sustainability Assessment of Combined Animal Fodder and Fuel Production from Microalgal Biomass.

We present a comparative environmental and social life cycle assessment (ELCA and SLCA) of algal fuel and fodder co-production (AF + fodder) versus algal fuel and energy co-production (AF + energy). Our ELCA results indicate that fodder co-production offers an advantage in the following categories: climate change (biogenic land use and land use change total), ecotoxicity, marine eutrophication, ionizing radiation, photochemical ozone creation, and land use. By contrast, the AF + energy system yields lower impacts in the other 11 out of 19 Environmental Footprint impact categories. Only AF + fodder offers greenhouse gas reduction compared to petroleum diesel (-25%). Our SLCA results indicate that AF + fodder yields lower impacts in the following categories: fair salaries, forced labor, gender wage gap, health expenditure, unemployment, and violation of employment laws and regulations. AF + energy performs favorably in the other three out of nine social indicators. We conclude that the choice of co-products has a strong influence on the sustainability of algal fuel production. Despite this, none of the compared systems are found to yield a consistent advantage in the environmental or social dimension. It is, therefore, not possible to recommend a co-production strategy without weighing environmental and social issues.

The role of hydrogen in heavy transport to operate within planetary boundaries.

Green hydrogen, i.e., produced from renewable resources, is attracting attention as an alternative fuel for the future of heavy road transport and long-distance driving. However, the benefits linked to zero pollution at the usage stage can be overturned when considering the upstream processes linked to the raw materials and energy requirements. To better understand the global environmental implications of fuelling heavy transport with hydrogen, we quantified the environmental impacts over the full life cycle of hydrogen use in the context of the Planetary Boundaries (PBs). The scenarios assessed cover hydrogen from biomass gasification (with and without carbon capture and storage [CCS]) and electrolysis powered by wind, solar, bioenergy with CCS, nuclear, and grid electricity. Our results show that the current diesel-based-heavy transport sector is unsustainable due to the transgression of the climate change-related PBs (exceeding standalone by two times the global climate-change budget). Hydrogen-fuelled heavy transport would reduce the global pressure on the climate change-related PBs helping the transport sector to stay within the safe operating space (i.e., below one-third of the global ecological budget in all the scenarios analysed). However, the best scenarios in terms of climate change, which are biomass-based, would shift burdens to the biosphere integrity and nitrogen flow PBs. In contrast, burden shifting in the electrolytic scenarios would be negligible, with hydrogen from wind electricity emerging as an appealing technology despite attaining higher carbon emissions than the biomass routes.

Comparative life cycle sustainability assessment of renewable and conventional hydrogen.

Hydrogen is gaining interest as a strategic element towards a sustainable economy. In this sense, sound decision-making processes in the field of hydrogen energy require thorough analyses integrating economic, environmental and social indicators from a life-cycle perspective. For this purpose, Life Cycle Sustainability Assessment (LCSA) constitutes an appropriate methodology jointly handling indicators related to the three traditional dimensions of the sustainability concept. In this work, the sustainability performance of renewable hydrogen from both wind-powered electrolysis and biomass gasification was benchmarked against that of conventional hydrogen from steam methane reforming under a set of five life-cycle indicators: global warming, acidification, levelised cost, child labour, and health expenditure. The results led to identify the stage of driving-energy/biomass production as the main source of impact. When compared to conventional hydrogen, the life-cycle sustainability performance of renewable hydrogen was found to underperform under social and economic aspects. Nevertheless, the expected enhancement in process efficiency would significantly improve the future performance of renewable hydrogen in each of the three main sustainability dimensions.

Prospective carbon footprint comparison of hydrogen options.

The Life Cycle Assessment methodology is often used to evaluate the environmental performance of hydrogen energy systems. However, even though hydrogen is usually seen as a strategic energy carrier for the future energy sector, there is a lack of case studies assessing its prospective life-cycle performance. In order to contribute to filling this gap, this work addresses a carbon footprint comparison of hydrogen options from a prospective standpoint. Four relevant hydrogen production pathways (steam methane reforming, grid-powered alkaline electrolysis, wind-powered alkaline electrolysis, and biomass gasification) under three time scenarios (reference, year 2030, and year 2050) are assessed, taking into account the expected evolution of key technical parameters such as efficiencies, lifespans, and the grid electricity mix. The results show a favourable carbon footprint of renewable hydrogen from biomass gasification and wind electrolysis, with a relatively steady near-zero carbon footprint. Despite the unfavourable carbon footprint results of conventional hydrogen from steam methane reforming and hydrogen from grid electrolysis, the latter is associated with a rapid trend towards a suitable long-term carbon footprint.

Linear discriminant analysis and principal component analysis to predict coronary artery disease.

Coronary artery disease is one of the most prevalent chronic pathologies in the modern world, leading to the deaths of thousands of people, both in the United States and in Europe. This article reports the use of data mining techniques to analyse a population of 10,265 people who were evaluated by the Department of Advanced Biomedical Sciences for myocardial ischaemia. Overall, 22 features are extracted, and linear discriminant analysis is implemented twice through both the Knime analytics platform and R statistical programming language to classify patients as either normal or pathological. The former of these analyses includes only classification, while the latter method includes principal component analysis before classification to create new features. The classification accuracies obtained for these methods were 84.5 and 86.0 per cent, respectively, with a specificity over 97 per cent and a sensitivity between 62 and 66 per cent. This article presents a practical implementation of traditional data mining techniques that can be used to help clinicians in decision-making; moreover, principal component analysis is used as an algorithm for feature reduction.

Robust eco-efficiency assessment of hydrogen from biomass gasification as an alternative to conventional hydrogen: A life-cycle study with and without external costs.

Hydrogen is a key product for the decarbonisation of the energy sector. Nevertheless, because of the high number of technical options available for hydrogen production, their suitability needs to be thoroughly evaluated from a life-cycle perspective. The standardised concept of eco-efficiency is suitable for this purpose since it relates, with a life-cycle perspective, the environmental performance of a product system to its value. Hence, this work benchmarks the eco-efficiency performance of renewable hydrogen produced through biomass gasification against conventional hydrogen from the steam reforming of natural gas. For the eco-efficiency assessment, the harmonised environmental indicators of global warming, acidification and cumulative non-renewable energy demand were individually used, while the product system value was based on the levelised cost of hydrogen with/without internalisation of the external socio-environmental costs associated with climate change and human health. On the one hand, when the environmental and economic performances are separately considered, hydrogen from biomass gasification performs significantly better than hydrogen from steam methane reforming under environmental aspects (e.g., greenhouse gas emissions saving of 98%), whereas the opposite conclusion was found from an economic standpoint (levelised cost of 3.59 € and 2.17 € per kilogramme of renewable and fossil hydrogen, respectively). On the other hand, when combining life-cycle environmental and economic indicators under the umbrella of the eco-efficiency assessment, it is concluded that the renewable hydrogen option outperforms the conventional one, which is further remarked when implementing socio-environmental externalities. In this regard, a relative eco-efficiency score above 14 was estimated for the renewable hydrogen option when benchmarked against conventional hydrogen.

Energy balance and life cycle assessment of a microalgae-based wastewater treatment plant: A focus on alternative biogas uses.

The techno-environmental performance of a medium-scale wastewater treatment system using high-rate algal ponds was evaluated through mass and energy balances and life cycle assessment. The system involves wastewater primary treatment, microalgae-based secondary treatment, thermal hydrolysis with steam explosion of microalgae, anaerobic co-digestion of pre-treated microalgal biomass and primary sludge, and biogas cogeneration. Furthermore, two scenarios based on alternative biogas uses were considered: (i) biogas for heat and electricity, and (ii) biogas for heat, electricity, and biomethane. Pumping wastewater to the primary settler arose as the main source of electricity consumption. When compared to conventional activated sludge plants, a large decrease in the energy consumption was observed for the secondary treatment. Moreover, a favourable life-cycle performance was generally found for the microalgae-based systems when displacing conventional energy products. Finally, the preference for a specific scenario on biogas use was found to be highly conditioned by the techno-environmental aspects prioritised by decision-makers.

Molecular identification and physiological characterization of yeasts, lactic acid bacteria and acetic acid bacteria isolated from heap and box cocoa bean fermentations in West Africa.

Yeast, lactic acid bacteria (LAB) and acetic acid bacteria (AAB) populations, isolated from cocoa bean heap and box fermentations in West Africa, have been investigated. The fermentation dynamicswere determined by viable counts, and 106 yeasts, 105 LAB and 82 AAB isolateswere identified by means of rep-PCR grouping and sequencing of the rRNA genes. During the box fermentations, the most abundant species were Saccharomyces cerevisiae, Candida ethanolica, Lactobacillus fermentum, Lactobacillus plantarum, Acetobacter pasteurianus and Acetobacter syzygii, while S. cerevisiae, Schizosaccharomyces pombe, Hanseniaspora guilliermondii, Pichia manshurica, C. ethanolica, Hanseniaspora uvarum, Lb. fermentum, Lb. plantarum, A. pasteurianus and Acetobacter lovaniensis were identified in the heap fermentations. Furthermore, the most abundant species were molecularly characterized by analyzing the rep-PCR profiles. Strains grouped according to the type of fermentations and their progression during the transformation process were also highlighted. The yeast, LAB and AAB isolates were physiologically characterized to determine their ability to grow at different temperatures, as well as at different pH, and ethanol concentrations, tolerance to osmotic stress, and lactic acid and acetic acid inhibition. Temperatures of 45 °C, a pH of 2.5 to 3.5, 12% (v/v) ethanol and high concentrations of lactic and acetic acid have a significant influence on the growth of yeasts, LAB and AAB. Finally, the yeastswere screened for enzymatic activity, and the S. cerevisiae, H. guilliermondii, H. uvarumand C. ethanolica species were shown to possess several enzymes that may impact the quality of the final product.

Efeito agudo de saltos intermitentes na arquitetura muscular do Vasto Lateral em atletas de voleibol / Acute effect of intermittent jumps on the Vastus Lateralis muscle architecture in volleyball players

O aumento do ângulo de penação de fibras musculares durante contração causa uma redução da geração de força do músculo. O objetivo deste estudo foi verificar a relação entre o ângulo de penação do músculo Vasto lateral (VL) com o rendimento em um teste de saltos verticais intermitentes. Foi testado um grupo de 18 atletas do sexo masculino da Confederação Brasileira de voleibol do Exército Brasileiro. Duas imagens longitudinais do VL, a 50% do comprimento da coxa, foram adquiridas com um Ultrassom modo-B. Posteriormente, os indivíduos realizaram um teste único do tipo Counter Movement Jump para avaliar a altura máxima do salto vertical. A tarefa de saltos verticais intermitentes compreendeu quatro séries de 15 segundos de saltos máximos. Imediatamente após a tarefa, duas imagens do ângulo de penação foram novamente adquiridas na mesma região. Duas medidas do ângulo de penação foram feitas em cada imagem, totalizando oito medições por indivíduo. Os resultados mostraram que houve redução significativa da altura dos saltos no decorrer da tarefa (35,77 ± 4,67 cm; 31,94 ± 4,51 cm; 27,04 ± 3,93 cm e 22,60 ± 3,66 cm para os intervalos de 0-15 s, 15-30 s, 30-45 s e 45-60 s, respectivamente). O ângulo de penação do VL não apresentou diferença significativa antes e após a realização da tarefa (12,72 ± 2,26º e 13,71 ± 3,40º, respectivamente). A correlação de Pearson entre as medidas do ângulo de penação no pré e pós teste intermitente e entre as alturas em todos os intervalos foram fracas (0,034

Increased pennation angle of the muscle fibers during contraction causes a reduced muscle force generation. The aim of this study was to investigate the relationship between the pennation angle of the vastus lateralis muscle (VL) with the performance of an intermittent vertical jumps test. We tested a group of 18 male athletes of the Brazilian Army Confederation of Volleyball. Two longitudinal images of the VL, at 50% of thigh length were acquired with a B-mode ultrasound. Soon after, subjects performed a Counter Movement Jump test to evaluate the maximum vertical jump height. The intermittent vertical jumps test comprised four sets of 15 s maximum jumps. Immediately after, the two images of the pennation angle were again obtained in the same region. Two pennation angle measurements were made in each image, totaling eight measurements per individual. The results of this study showed that there was a significant reduction of the jumps heights (35.77 ± 4.67 cm; 31.94 ± 4.51 cm; 27.04 ± 3.93 cm and 22.60 ± 3 66 cm intervals for 0-15 s, 15-30 s, 30-45 s and 45-60 s, respectively). The VL pennation angle did not present a significant difference before and after the test (12.72 ± 2.26 ° and 13.71 ± 3.40º respectively). The Pearson correlation between the pennation angle measurements before and after the intermittent test and among the heights in all intervals were weak (0.034

Análise do impacto do tênis e coturno fornecidos pelo exército brasileiro durante a marcha / Analysis of shock absortion during gait wearing tennis shoes and military boots from Brazilian Army

O objetivo do presente estudo foi comparar a absorção de impacto durante a marcha descalço e com tênis e coturno fornecidos pelo Exército Brasileiro. A amostra foi composta por 20 soldados saudáveis recém-incorporados sem sintomas ou lesões musculoesqueléticas ou neurológicas que interferissem na marcha. Os sujeitos foram instruídos a caminhar em uma plataforma de madeira de 10 metros de comprimento por um metro de largura, com duas plataformas de força (Bertec, EUA) embutidas no centro. O componente vertical da força de reação do solo foi avaliado durante a marcha nas situações tênis, coturno e descalço. O sistema de cinemetria (Qualysis, Suécia) foi utilizado para medir a velocidade da marcha em cada situação de teste. O primeiro pico de força (PPF), o tempo para atingir o PPF (T_PPF), pico transiente de impacto (IPF) e a taxa de aceitação do peso (TAP) entre 10% e 30%, 30% e 50%, 50% e 70%, 70% e 90% e 10% e 90% do PPF foram avaliadas. O T_PPF foi em média 1s menor (p < 0,0001), o IPF e a TAP entre 10% e 30% do PPF foram maiores (p < 0,0001, ambos) descalço comparados a marcha com tênis e coturno. Não foi observado diferença estatística nas outras variáveis estudadas nas três condições de teste, bem como em nenhuma variável na comparação entre tênis e coturno. Os calçados avaliados são eficientes em reduzir o impacto durante a marcha por atrasar em média 1 s o tempo em que o PPF foi atingido, reduzir o IPF e a TAP nos primeiros 30% do PPF

This study aimed at comparing shock absorption during gait while walking barefoot, tennis shoes and military boots. Twenty healthy soldiers without neurologic or musculoskeletal disorders were evaluated. Subjects walked 10 meters along a straight and level walkway at a comfortable speed in barefoot condition and using tennis shoes and military boots. Two force platforms (Bertec, EUA) were placed in serial at the middle of the walkway used to measure the ground reaction forces (GRF). The kinematic system (Qualysis, Sweden) was used to measure gait speed in each test condition. The first peak of force (FPF) of the vertical component of the GRF, time to reach to FPF (T_FPF), transient impact peak force (IPF) were evaluated and the weight acceptance rate at 10% to 30%, 30% to 50%, 50% to 70%, 70% to 90% and 10% to 90% of FPF. T_FPF was 1 s shorter (p < 0.0001), as well as IPF and weight acceptance rate at 10% to 30% in barefoot was higher (p < 0.0001, both) than tennis shoes and military boots gait patterns. None statistical difference was found in the others analyzed variables, even as in the tennis shoes and military boots comparisons. The analyzed footwear delayed the FPF impact during gait, reduce the IPF and the weight acceptance phase during 10% to 30% of FPF, being efficient in reduce impact forces during gait

Association between symptom severity and internal capsule volume in obsessive-compulsive disorder.

Neurobiological models support an involvement of white matter tracts in the pathophysiology of obsessive-compulsive disorder (OCD), but there has been little systematic evaluation of white matter volumes in OCD using magnetic resonance imaging (MRI). We investigated potential differences in the volume of the cingulum bundle (CB) and anterior limb of internal capsule (ALIC) in OCD patients (n=19) relative to asymptomatic control subjects (n=15). White matter volumes were assessed using a 1.5T MRI scanner. Between-group comparisons were carried out after spatial normalization and image segmentation using optimized voxel-based morphometry. Correlations between regional white matter volumes in OCD subjects and symptom severity ratings were also investigated. We found significant global white matter reductions in OCD patients compared to control subjects. The voxel-based search for regional abnormalities (with covariance for total white matter volumes) showed no specific white matter volume deficits in brain portions predicted a priori to be affected in OCD (CB and ALIC). However, large clusters of significant positive correlation with OCD severity scores were found bilaterally on the ALIC. These findings provide evidence of OCD-related ALIC abnormalities and suggest a connectivity dysfunction within frontal-striatal-thalamic-cortical circuits. Further studies are warranted to better define the role of such white matter alterations in the pathophysiology of OCD, and may provide clues for a more effectively targeting of neurosurgical treatments for OCD.

Regional gray matter abnormalities in obsessive-compulsive disorder: a voxel-based morphometry study.

BACKGROUND: Several structural magnetic resonance imaging (MRI) studies have investigated the presence of brain abnormalities in obsessive-compulsive disorder (OCD) but have not produced consistent findings. This might be partly related to their use of a regions-of-interest approach. We assessed gray matter volumes in 19 OCD subjects and 15 healthy volunteers, using voxel-based morphometry (VBM). METHODS: Images were acquired with a 1.5-T MRI scanner, spatially normalized, and segmented with optimized VBM. Statistical comparisons were performed with the general linear model. RESULTS: Significant findings were detected in regions predicted a priori to be implicated in OCD, including increased gray matter in OCD subjects relative to control subjects in posterior orbitofrontal and parahippocampal regions; decreased gray matter in OCD patients in the left anterior cingulate cortex; and inverse correlations between obsessive-compulsive symptom severity and gray matter in the medial thalamus (p < .001, uncorrected for multiple comparisons). Also, an unpredicted site of gray matter reduction in OCD patients in the right parietal associative cortex approached significance (p = .052, corrected for multiple comparisons). CONCLUSIONS: Our findings are consistent with previous studies implicating dysfunction of orbitofrontal, cingulate, thalamic, and temporolimbic regions in OCD and suggest that the involvement of the parietal cortex in the pathophysiology of OCD warrants further investigation.

Estudo experimental de 36 projéteis de arma de fogo quanto à esterilizaçäo e possibilidade de carreamento de bactérias após disparo / Experimental study of 36 gunshots in terms of sterilization and possibility of bacteria transport after shooting

Os autores estudaram experimentalmente três grupos de 12 disparos de armas de fogo calibre 38. O grupo 1 utilizou muniçao comum sem nenhum preparo e serviu como controle; no grupo 2, provocou-se contaminaçao da muniçao com bactérias de E. coli e S. aureus padronizadas; e no grupo 3, utilizou-se muniçao comum, mas transfixando tecido contaminado pelas mesmas bactérias. Nos grupos 1 e 2, nao foi observado crescimento bacteriano em nenhum meio de cultura, fortalecendo a hipótese da esterilizaçao do projétil pelo disparo. No grupo 3, houve crescimento bacteriano tanto num meio de cultura sólido quanto num líquido, demonstrando que um projétil se contamina ao atravessar tecido contaminado, carreando bactérias no seu trajeto.