Wobble tRNA modification and hydrophilic amino acid patterns dictate protein fate.

Regulation of mRNA translation elongation impacts nascent protein synthesis and integrity and plays a critical role in disease establishment. Here, we investigate features linking regulation of codon-dependent translation elongation to protein expression and homeostasis. Using knockdown models of enzymes that catalyze the mcm5s2 wobble uridine tRNA modification (U34-enzymes), we show that gene codon content is necessary but not sufficient to predict protein fate. While translation defects upon perturbation of U34-enzymes are strictly dependent on codon content, the consequences on protein output are determined by other features. Specific hydrophilic motifs cause protein aggregation and degradation upon codon-dependent translation elongation defects. Accordingly, the combination of codon content and the presence of hydrophilic motifs define the proteome whose maintenance relies on U34-tRNA modification. Together, these results uncover the mechanism linking wobble tRNA modification to mRNA translation and aggregation to maintain proteome homeostasis.

Measured and derived data at the exhaust of a batch industrial dryer.

This work reports the measured and derived quantities of an experimental campaign conducted in an existing laundry to record the conditions of the exhaust air of a batch industrial natural gas-fired dryer for cotton flat fabrics, like bed linens or tablecloths. The load of fabrics is 85 kg, referred to the dry fabric. The measured quantities are temperature and velocity in the center of a squared exhaust duct, which are reported along with their measurement uncertainty. The derived quantity is the exhaust air mass flow rate along with its combined uncertainty. In particular, these data can be used to study the feasibility of waste heat recovery strategies, such as that studied in the related research article "Optimal cascade phase change regenerator for waste heat recovery in a batch industrial dryer" [1].

Experimental data of the aqueous NH3 and CO2 absorption at temperatures from 15 °C to 35 °C, NH3 concentrations from 5% to 15% and CO2 loadings from 0.2 to 0.6 measured with the Wetted Wall Column.

The absorption between aqueous NH3 and CO2 is studied using the Wetted Wall Column in order to show the effect of the solvent condition on the rate of reaction. A total of 27 different cases are investigated in the region defined by temperatures from 15 °C to 35 °C, NH3 concentrations from 5% to 15% and CO2 loadings from 0.2 to 0.6. The paper reports the data measured during the experiments, the experimental apparatus description and the experimental procedure. The data here presented are both the raw data measured with their uncertainty and the final value of the overall mass transfer coefficient. The overall mass transfer coefficient is the result of the raw data treatment explained in the research paper related to this data. The data here reported are analyzed in the paper by Lillia et al. (2018) [1].