RESUMEN
The by-catch fauna of the shrimp fishery includes a number of marine invertebrates that are discarded because they do not have commercial value. In order to try to add some value to these materials, we analyzed the chemical composition of the starfish Luidia senegalensis collected in the Brazilian coast as a consequence of the trawling fishery method. In order to access their chemical composition, we used a combination of solid phase extraction (SPE) followed by ultra-high performance liquid chromatography coupled to electrospray ionization ion trap tandem mass spectrometry (UPLC-ESI-IT-MSn). Luidia senegalensis contains asterosaponins, which are sulphated glycosilated steroids, containing five and six sugar moieties, in addition to polyhydroxysteroids. This study helped us to support the presence of important and potentially bioactive compounds in invertebrates associated to the by-catch fauna of the shrimp fishery, using a fast and efficient method.
RESUMEN
We investigated modulation by ATP, Mg²âº, Naâº, K⺠and NH4⺠and inhibition by ouabain of (Naâº,Kâº)-ATPase activity in microsomal homogenates of whole zoeae I and decapodid III (formerly zoea IX) and whole-body and gill homogenates of juvenile and adult Amazon River shrimps, Macrobrachium amazonicum. (Naâº,Kâº)-ATPase-specific activity was increased twofold in decapodid III compared to zoea I, juveniles and adults, suggesting an important role in this ontogenetic stage. The apparent affinity for ATP (K(M) = 0.09 ± 0.01 mmol L⻹) of the decapodid III (Naâº,Kâº)-ATPase, about twofold greater than the other stages, further highlights this relevance. Modulation of (Naâº,Kâº-ATPase activity by K⺠also revealed a threefold greater affinity for K⺠(K0.5 = 0.91 ± 0.04 mmol L⻹) in decapodid III than in other stages; NH4⺠had no modulatory effect. The affinity for Na⺠(K0.5 = 13.2 ± 0.6 mmol L⻹) of zoea I (Naâº,Kâº)-ATPase was fourfold less than other stages. Modulation by Naâº, Mg²âº and NH4⺠obeyed cooperative kinetics, while K⺠modulation exhibited Michaelis-Menten behavior. Rates of maximal Mg²âº stimulation of ouabain-insensitive ATPase activity differed in each ontogenetic stage, suggesting that Mg²âº-stimulated ATPases other than (Naâº,Kâº)-ATPase are present. Ouabain inhibition suggests that, among the various ATPase activities present in the different stages, Naâº-ATPase may be involved in the ontogeny of osmoregulation in larval M. amazonicum. The NH4âº-stimulated, ouabain-insensitive ATPase activity seen in zoea I and decapodid III may reflect a stage-specific means of ammonia excretion since functional gills are absent in the early larval stages.