Characterization of the biosurfactant production and enzymatic potential of bacteria isolated from an oil-contaminated saline soil / Caracterización de la producción de biosurfactantes y potencial enzimático de bacterias aisladas de un suelo salino contaminado con aceite

Biosurfactants are amphiphilic compounds with extensive applications in oily contaminated environments to remove hydrocarbons. Moreover, enzymes such as laccase and manganese peroxidase are responsible for the oxidation of a variety of phenolic compounds and aromatic amines. Therefore, in the present study, bacteria with the potential to produce biosurfactants and enzymes (namely, laccase, manganese peroxidase, and endoglucanase carboxymethyl cellulose (CMCase)) were isolated from petroleum oil-contaminated soil. From 15 isolated bacteria, three isolates were selected as the best producers of biosurfactants according to the related tests, such as tests for surface tension reduction. These three bacteria indicated tolerance to a salinity test and were classified as resistant and very resistant. The isolates 3, 12, 13, and 14 showed positive results for the degradation of guaiacol, phenol red, and carboxymethylcellulose, as well as the decoloration of methylene blue by the creation of a clear halo around the bacterial colony. Upon the quantitation of the laccase and manganese peroxidase activities, 22.58 U/L and 21.81 U/L, respectively, were measured by isolate 13. Furthermore, CMCase activity was recorded with 0.057436 U/ml belonging to isolate 14. Bacterial strains with appreciable laccase, peroxidase, CMCase activity, and biosurfactant production potentials were identified through 16S rDNA sequence analysis as Bacillus sp. (isolate 3), Bacillus toyonensis (isolate 12), Bacillus cereus (isolate 13), and Bacillus tropicus (isolate 14), and their nucleotide sequences were deposited in the GenBank. The potentials for the industrial applicability of the biosurfactants and enzymes abound, and production needs to be optimized by the selected bacterial strains.(AU)

Influence of nonthermal argon plasma on the micro-shear bond strength between resin cement and translucent zirconia.

Background: Considering the potential of translucent zirconia for application in esthetic restorations, it is necessary to find effective methods with the least adverse effects to increase its bond strength to resin cement. Aims: This study aimed to test if different conservative surface treatments and cement types could affect the micro-shear bond strength (µSBS), failure mode, and bonding interface between resin cement and translucent zirconia. Materials and Methods: In this in vitro experimental study, translucent zirconia blocks were divided into four groups based on the surface treatment they received: no treatment, argon plasma, primer (Pr), and Pr + plasma. Each group was further divided into two subgroups based on the applied cement: PANAVIA F2 and Duo-Link cement. Fourteen cement columns with a diameter of 1 mm were placed on each block (n = 14); all the specimens were immersed in 37°C water for 24 h. Afterward, µSBS was evaluated (P < 0.05), and the mode of failure was determined by a stereomicroscope (×10). The cement-zirconia interface and the surface hydrophilicity (contact angle) were also evaluated. Statistical Analysis: Two-way analysis of variance (ANOVA) was used to evaluate the effect of surface preparation, cement types, and incubator, simultaneously (P < 0.05). The bond strengths after incubation were analyzed by one-way ANOVA (P < 0.05). Failure mode, contact angle, and cement-zirconia interface were analyzed descriptively. Results: The highest bond strength was seen in Pr surface treatment for Duo-Link cement; however, this group was not significantly different from Pr and PANAVIA F2 cement and Pr + plasma and Duo-Link cement (P = 0.075) groups. All plasma specimens in the incubator failed prematurely. The mode of failure in all specimens was adhesive. The lowest and highest contact angles were seen in Pr + plasma and the control groups, respectively. Conclusion: The use of Pr could successfully improve the bond strength of resin cement to translucent zirconia while plasma was not an acceptable and durable substitute.

Effect of pretreatment of root dentin surface with cold atmospheric plasma on improving the bond strength of fiber post and resin cement: In vitro study.

INTRODUCTION: Achieving stable adhesion between fiber post and interradicular dentin is a challenging process in the restoration of endodontically treated teeth. This study was conducted to investigate the effect of surface pretreatment with cold atmospheric plasma (CAP) on improving the bond strength between them. MATERIALS AND METHODS: Forty-eight single-canal mandibular premolars were cut 1 mm above the cementoenamel junction to keep the root length of 14 mm or more. After endodontic treatment and preparation of the post space, the teeth were divided into four groups regarding the pretreatment of dentin surfaces, including normal saline, ethylenediaminetetraacetic acid (EDTA), CAP, and CAP + EDTA groups. The data were analyzed using paired and independent t-test and one-way analysis of variance and the significance level was set at p < .05. RESULTS: The bond strength was significantly higher in the coronal third than in the apical third in all the groups. Moreover, the bond strength was significantly higher in the CAP + EDTA-treated group. The bond strength increased significantly in the CAP group compared to the normal saline group. In addition, the bond strength increased significantly in the CAP or EDTA groups compared to the control group. The lowest bond strength belonged to the control group (normal saline). CONCLUSION: The surface pretreatment with CAP (alone or in combination with EDTA) played a significant role in improving the bond strength of fiber post and root canal dentin.

Effect of Nonthermal Plasma on Shear Bond Strength of Translucent Zirconia in Layering Ceramic.

Background: Today, various methods are used to increase the bond strength of zirconia in layering ceramics. This study evaluated the effects of nonthermal argon plasma on zirconia shear bond strength to layering porcelain. Materials and Method. In this experimental study, 42 square blocks of zirconia were prepared and randomly divided into three groups (n = 14) according to the applying surface treatment: (1) the control group (without any surface treatment), (2) the plasma-treated group with argon nonthermal plasma, and (3) the air abrasion group with 50 µm Al2O3 particles. All samples were layered with porcelain. One sample from each group was evaluated by electron microscopy (SEM) to examine the cross-sectional area of the zirconia-ceramic bond. The rest of the specimens were subjected to thermocycling with 5,000 baths to imitate the aging process in the mouth and then were tested for shear bond strength. The failure pattern of the samples was examined by stereomicroscope. Bond strength data were analyzed by one-way ANOVA test in three groups and Tamhane post hoc test in pairs. The significance level of p-value was considered 0.05. Results: The shear bond strength of the plasma-treated group was significantly higher than the control group (p = 0.032) but the shear bond strength between the sandblast and the plasma-treated group was not significantly different (p = 0.656). The shear bond strength between the sandblast and the control group was also not significant (p = 0.202). Regarding the mode of failure, failures were mostly adhesive and then mixed. Examination of the samples under SEM showed that the bond area is the thickest in the sandblast group and also the surface roughness is the highest in the sandblast group and the lowest in the control group. Conclusion: This study demonstrated that the use of nonthermal argon plasma treatment is an effective way to enhance the quality and quantity of shear bond strength between layering porcelain and zirconia.

Characterization of the biosurfactant production and enzymatic potential of bacteria isolated from an oil-contaminated saline soil.

Biosurfactants are amphiphilic compounds with extensive applications in oily contaminated environments to remove hydrocarbons. Moreover, enzymes such as laccase and manganese peroxidase are responsible for the oxidation of a variety of phenolic compounds and aromatic amines. Therefore, in the present study, bacteria with the potential to produce biosurfactants and enzymes (namely, laccase, manganese peroxidase, and endoglucanase carboxymethyl cellulose (CMCase)) were isolated from petroleum oil-contaminated soil. From 15 isolated bacteria, three isolates were selected as the best producers of biosurfactants according to the related tests, such as tests for surface tension reduction. These three bacteria indicated tolerance to a salinity test and were classified as resistant and very resistant. The isolates 3, 12, 13, and 14 showed positive results for the degradation of guaiacol, phenol red, and carboxymethylcellulose, as well as the decoloration of methylene blue by the creation of a clear halo around the bacterial colony. Upon the quantitation of the laccase and manganese peroxidase activities, 22.58 U/L and 21.81 U/L, respectively, were measured by isolate 13. Furthermore, CMCase activity was recorded with 0.057436 U/ml belonging to isolate 14. Bacterial strains with appreciable laccase, peroxidase, CMCase activity, and biosurfactant production potentials were identified through 16S rDNA sequence analysis as Bacillus sp. (isolate 3), Bacillus toyonensis (isolate 12), Bacillus cereus (isolate 13), and Bacillus tropicus (isolate 14), and their nucleotide sequences were deposited in the GenBank. The potentials for the industrial applicability of the biosurfactants and enzymes abound, and production needs to be optimized by the selected bacterial strains.

Effect of conventional and power office bleaching with diode laser and led light on enamel microhardness

Aim: The present study aimed to asses enamel microhardness after office bleaching with diode laser and LED light compared to the conventional bleaching procedure. Methods: Thirty-nine human premolar teeth were collected and randomly divided into three groups regarding of the bleaching technique. Group 1: Snow O bleaching gel with LED light-curing unit; Group 2: Snow L bleaching gel with diode laser irradiation; and Group 3: Opalescence Boost bleaching gel with no light source in group 3. Enamel surface changes were evaluated in one tooth in each study group and one intact tooth as a reference under a scanning electron microscope (SEM). In the remaining samples (n=12), enamel microhardness was determined by Vickers microhardness test before and after bleaching. Data were analyzed with repeated-measures ANOVA to compare microhardness changes, followed by post hoc Tukey tests at the 0.05 significance level. Results: Enamel microhardness decreased in all the groups after bleaching, with the maximum decrease in microhardness in the Snow O bleaching group with LED light, which was significantly higher than the other groups (P=0.002). The two other groups did not exhibit any significant difference in microhardness decrease (P>0.05). Conclusion:Based on the limitations of this study, it can be concluded power bleaching with 980nm diode laser was less time-consuming compare to conventional bleaching procedure and yielded better outcomes in terms of enamel surface microhardness compared to the use of an LED light-curing unit

Effect of glazing and polishing on opalescence and fluorescence of dental ceramics.

OBJECTIVE: Tooth enamel has opalescence and fluorescence, which should be mimicked by esthetic dental restorations. The purpose of this study was to compare the effects of glazing and polishing on the opalescence and fluorescence of dental ceramics. MATERIALS AND METHODS: Twenty-four discs were fabricated of feldspathic, IPS e.max, zirconia, and Enamic ceramics with 10 mm diameter and 0.5 and 1 mm thicknesses (n = 12). Of the discs fabricated with 0.5 and 1 mm thicknesses, half of them were glazed and the remaining half were polished (n = 6). Opalescence was calculated as the difference in yellow-blue (CIE ∆b*) and red-green (CIE ∆a*) color axes between the transmitted and reflected colors. The fluorescence of specimens was measured by a novel technique. Data were analyzed using two-way analysis of variance at a 0.05 level of significance. RESULTS: In all groups (except for the Enamic ceramic), the mean opalescence of polished specimens (e.max = 2.704, feldspathic = 1.67, zirconia = 3.143) was higher than that of glazed specimens (e.max = 2.163, feldspathic = 1.016, zirconia = 2.690). The mean opalescence of glazed Enamic specimens (2.140) was higher than that of polished specimens (1.308). The fluorescence of glazed and polished specimens was not significantly different. CONCLUSION: Surface treatment (glazing/polishing) affects the opalescence, but not the fluorescence of dental ceramics evaluated in this study.

Forced Eruption: Alternative Treatment Approach to Restore Teeth with Subgingival Structure.

The management of teeth with deep caries, fracture, or perforation in the cervical third of the root is an integral part of dental practice. Orthodontic extrusion preserves the natural root system and may convert the tooth indicated for extraction into useful tooth with good prognosis and a low risk of failure. Orthodontic tooth eruption can be an alternative to treatment such as surgical crown lengthening, especially in esthetic areas, and provides more favorable conditions for prosthodontic coronal restorations by guaranteeing proper sealing and esthetics and preserving periodontal tissue health. The aim of this case report was to explain a multidisciplinary approach that successfully preserve and treat the teeth with subgingival carious lesion. This innovative method is cost-effective and can be easily done with the equipment available in any office.

Evaluation of Microhardness of Two Bulk-fill Composite Resins Compared to a Conventional Composite Resin on surface and in Different Depths.

Statement of the Problem: One of the problems with light-cured composite resins is the limitation and inadequate depth of curing and polymerization, resulting in low surface microhardness and restoration failure. Purpose: The present study aimed to compare the surface microhardness of two different bulk-fill composite resins and one conventional composite resin using the Vickers microhardness test. Materials and Method: In the present in vitro study, 108 samples from two different bulk-fill composite resins (Tetric N Ceram and Xtrafil) and one conventional composite resin (Filtek Z250) were prepared in metallic molds (2×4×10 mm) (n=36 for each composite resin). Six samples from each composite resin (n=6) underwent a hardness measurement test at specific depths (0.1, 1, 2, 3, 4 and 5mm). The samples were then stored at 37ºC for 24 hours, followed by a microhardness test at the depths mentioned above. Results: In all the composite resin samples, microhardness decreased with an increase in depth. The highest microhardness was recorded in Filtek Z250, followed by Xtrafil, with no significant difference. The lowest microhardness was recorded in Tetric N Ceram bulk-fill. Both bulk-fill composite resins at all the depths exhibited depth-to-surface standard microhardness (>80%). Conclusion: According to the results, both evaluated bulk-fill composite resins exhibited favorable surface microhardness up to a depth of 5 mm.

Evaluating the relation of posterior occlusal plane to ala-tragal line according to age and sex.

INTRODUCTION: Occlusal plane orientation affects the esthetic, function, and success of removable or fixed prostheses. The most prevalent index for determination of this plane is the ala-tragus line while there are controversies in the exact location of tragus reference point.

Microhardness change of human dental enamel due to power bleaching with different gels.

AIM: Since the introduction of bleaching treatments in the office, different lights have been suggested to accelerate the bleaching reaction. This study aimed to evaluate the microhardness of tooth enamel after office bleaching using different materials.

AMELX and ENAM Polymorphisms and Dental Caries.

Introduction: The variety of the genetic factors playing role in development of dental caries calls for further research in this regard. The aim of the present study was to investigate the differences between caries-free adults and adults with dental caries in terms of polymorphism of caries-related genes (AMELX and ENAM). Methods: The present case-control study was performed on 81 adults aged 18-24 years, 41 caries free, and 40 with a DMFT ≥ 4. A questionnaire containing background and demographic information (such as age, gender, time and type of latest dental check-ups, parent's education, oral self-care, and the place of residence in the first 12 years of life) was completed by participants at the time of examination. The blood sample was taken from each participant in the EDTA tube, and PCR was performed. Gene diversity of AMELX and ENAM genes was compared between the two groups. Results: Regarding AMELX gene, in the caries-free group 33 (80.5%) and in the group with DMFT ≥ 4, 33 (82.5%) students had TT genotype, but this difference was insignificant. For ENAM gene, in the caries-free group 34 (82.9%) and in the group with DMFT ≥ 4, 39 (97.5%) students had TT genotype, but this difference was insignificant (P value = 0.048, CI 95%:0.02-1.27, and OR = 0.145). Conclusion: There was no relationship between TT and TC genotypes of single nucleotide polymorphism of AMELX and ENAM gene and susceptibility to dental caries, but with increasing sample size, there may be a relationship between SNP of ENAM gene and being caries free.

Bacterial leakage assessment in root canals sealed with AH Plus sealer modified with silver nanoparticles.

BACKGROUND: Successful endodontic therapy requires prevention of bacterial leakage between the root canal filing and root-canal walls. Sealing quality of a root canal filling depends strongly on the sealing ability of the sealer used. The present study aimed to evaluate the bacterial leakage resistance of AH Plus sealer modified with silver nanoparticles. METHODS: Forty sound teeth were obturated using lateral compaction technique except for five teeth as the negative controls. After considering five teeth as the positive controls (filled without sealer), the remaining teeth were assigned to two groups (n = 15) in terms of the sealer used (AH Plus sealer and silver nanoparticle-modified AH Plus). Bacterial leakage was evaluated in saliva using the two-chamber technique in every 24 h. When all the samples in the test groups were contaminated, the study was terminated. The data were analyzed with log-rank statistical test. RESULTS: All samples in both experimental groups were contaminated during the 3-months period of observation. There was no significant difference in contamination time between study groups (P > 0.05). CONCLUSIONS: Silver nanoparticles used in tested concentration did not improve the bacterial leakage resistance of AH Plus sealer.

Rehardening of Eroded Enamel with CPP-ACFP Paste and CO2 Laser Treatment.

BACKGROUND: Diet and lifestyle can destroy tooth structure due to the dissolution of enamel by acidic beverages. The present study evaluated the effect of CO2 laser irradiation and CPP-ACFP (casein phosphopeptide and amorphous calcium phosphate with fluoride) paste on the remineralization of enamel eroded by carbonated soft drinks. METHODS: In the present in vitro study, 46 human sound premolar teeth were sectioned mesiodistally to achieve 84 samples. Fourteen samples were assigned to the positive control group (G1), and the remaining samples were immersed in 500 mL of cola drink for 2 minutes, followed by rinsing with distilled water for 10 seconds. This procedure was carried out three times to create erosive lesions. Then, the 60 eroded samples were randomly assigned to five groups of G2 to G6 in terms of the treatment as follows: negative control (G2), CO2 laser irradiation (G3), CPP-ACFP paste (G4), CO2 laser irradiation followed by CPP-ACFP paste application (G5), and CPP-ACFP paste application followed by CO2 laser irradiation (G6). The mean surface microhardness of the enamel surface was evaluated and determined at three points for each sample. Data were analyzed with one-way ANOVA and Tukey HSD tests (α = 0.05). RESULTS: The highest and the lowest hardness values were recorded in the G1 (314 ± 12 kg/mm2) and G2 (213.7 ± 12 kg/mm2) groups, respectively. ANOVA revealed significant differences between the study groups (P < 0.001). Two-by-two comparisons showed significant differences between the G2 group and the other groups, indicating the efficacy of all the treatment modalities in tooth remineralization and rehardening procedures (P < 0.05). Only in group G6, the enamel microhardness was not significantly different from the G1 positive control group (P > 0.05). CONCLUSION: Considering the parameters used in the present study, CO2 laser irradiation or CPP-ACFP paste application alone increased eroded enamel's surface hardness; however, their sequential application was more effective in rehardening the eroded enamel's surface to near-normal levels.

Evaluation of Antibacterial Effects of Fissure Sealants Containing Chitosan Nanoparticles.

OBJECTIVES: The present study evaluated the antimicrobial effects of fissure sealants containing chitosan nanoparticles. MATERIALS AND METHODS: Antibacterial effect of Master Dent fissure sealant alone and after incorporating chitosan nanoparticles was evaluated on Streptococcus mutans, sanguis, and Lactobacillus acidophilus. Biofilm growth was evaluated by determining colony counts. Antimicrobial effect was determined on days 3, 15, and 30 by counting microbial colonies using eluted components test. One-way ANOVA, Tukey HSD tests, t test, and two-way ANOVA were used for statistical analyses (α = 0.05). RESULTS: Biofilm inhibition test showed that fissure sealant containing 1 wt.% chitosan decreased colony counts significantly (P < 0.05). Eluted components test with S. mutans and sanguis showed significant decrease in colony counts during the first 15 days in chitosan containing group; however, from day 30, antimicrobial activity decreased noticeably, with no significant difference from control group (P > 0.05). Antimicrobial activity against L. acidophilus was maintained in chitosan group up to 30 days, and decrease in colony counts was significant (P < 0.05). CONCLUSION: According to the results of this study, incorporation of 1 wt.% chitosan into fissure sealant induced an antimicrobial activity. Antibacterial effect on L. acidophilus persisted for longer time (30 days) compared to the two other bacterial species (15 days).

Effects of Universal Adhesive on Shear Bond Strength of Resin Cement to Zirconia Ceramic with Different Surface Treatments.

AIM: This study aimed to assess shear bond strength (SBS) of resin cement to zirconia ceramic with different surface treatments by using Single Bond Universal. METHODS: In this in vitro study, 50 zirconia discs (2 × 6 mm) were divided into 5 groups of (I) sandblasting with silica-coated alumina (CoJet) + silane + Single Bond 2, (II) sandblasting with CoJet + Single Bond Universal, (III) sandblasting with alumina + Single Bond Universal, (IV) sandblasting with alumina + Z-Prime Plus, and (V) Single Bond Universal with no surface treatment. Resin cement was applied in plastic tubes (3 × 5 mm2), and after 10,000 thermal cycles, the SBS was measured by a universal testing machine. The mode of failure was determined under a stereomicroscope at × 40 magnification. Data were analyzed using one-way ANOVA. RESULTS: The maximum (6.56 ± 4.29 MPa) and minimum (1.94 ± 1.96 MPa) SBS values were noted in groups III and I, respectively. Group III had the highest frequency of mixed failure (60%). Group V had the maximum frequency of adhesive failure (100%). CONCLUSION: Single Bond Universal + sandblasting with alumina or silica-coated alumina particles is an acceptable method to provide a strong SBS between resin cement and zirconia.

Phosphate Ion Release and Alkalizing Potential of Three Bioactive Dental Materials in Comparison with Composite Resin.

AIM: Several new bioactive compounds were recently introduced to the market with favorable ion release, tooth remineralization, and alkalizing potential. This study sought to compare the phosphate ion release and alkalizing potential of three bioactive materials in comparison with composite resin. METHODS: Thirty-six discs (2 × 6 mm) were fabricated from Fuji II LC resin modified glass ionomer (RMGI), Activa BioActive, Cention N, and Z250 composite in plastic molds. The specimens were stored in distilled water for 24 and 48 h and 6 months. Half of the specimens were used to assess the phosphate ion release while the other half were used to assess the alkalizing potential 1 h after pH drop from 6.8 to 4. Phosphate ion release was quantified by a spectrophotometer while the pH value was measured by a pH meter. Data were analyzed using two-way ANOVA, one-way ANOVA, and Tukey's HSD test (for pairwise comparisons) at 0.05 level of significance. RESULTS: At 24 h, the maximum phosphate ion release in distilled water occurred in the Fuji II LC group followed by Cention N, Activa BioActive, and Z250. At 6 months, Cention N followed by Activa BioActive showed higher phosphate ion release than Fuji II LC and Z250. No significant difference was noted between Activa BioActive and Cention N at any time point. All materials, except for Z250, increased the pH of the environment. Fuji II LC had maximum alkalizing effect at all time points followed by Cention N and Activa BioActive. CONCLUSION: Use of bioactive compounds is a promising method to ensure phosphate ion release, and can have a positive effect on tooth remineralization over time. Also, bioactive compounds can alkalize an acidic environment.

Finite element stress analysis of composite resin veneers for diastema closure.

The fracture of restorations used for diastema closure is a clinical concern. The objective of this study was to perform a finite element-based comparative analysis of functional stress patterns in composite resin veneer restorations used for diastema closure to determine the influence of factors such as the preparation design, proximal extension, loading level, and vector of stress (loading angle). Three-dimensional finite element stress analysis was performed on a maxillary central incisor model to examine the stress distribution resulting from a nanofilled composite veneer restoration with 4 different extensions of unsupported composite (0.5, 1.0, 1.5, or 2.0 mm) and 2 different preparation designs (partial or full) under 3 loads (50, 150, or 250 N) and 3 loading angles (60°, 90°, or 125°). The maximum stress was found to be concentrated on the full-preparation design with a 0.5-mm mesial extension under a 250-N load and 60° loading angle. The minimum stress was found with the partial-preparation design with a 0.5-mm mesial extension under a 50-N load and 90° loading angle. Based on the results of the present study, a partial-preparation design is preferred when nanofilled composite resins are used for diastema closure.

Influence of resin cement on color stability of ceramic veneers: in vitro study.

STATEMENT OF THE PROBLEM: Long-term success of ceramic veneers depends on the color stability of resin cement used for their cementation. Color change of cement may be seen through the ceramic and compromise esthetics. PURPOSE: This study aimed to compare the color change of two resin cements and their visibility through the ceramic veneers after accelerated artificial aging. MATERIALS AND METHODS: In this in vitro study, color change (ΔE) was measured in the following groups (n = 10) before and after accelerated artificial aging: group 1, IPS e.max press high translucent ceramic discs; groups 2, Variolink NLC resin cement discs; group 3, Choice 2 resin cement discs; groups 4, Variolink NLC discs bonded to e.max ceramic discs; group 5, Choice 2 disc bonded to e.max ceramic discs. Color change was measured using a spectrophotometer according to International Commission on Illumination Lab (CIELab). Data were analyzed using one-way ANOVA and Tukey's post-hoc test. RESULTS: Group 2 showed the highest (ΔE = 10.4 ± 0.9) and group 1 the lowest (ΔE = 0.9 ± 0.4) color change. The color change of Variolink was significantly greater than Choice 2 either alone (p < .001) or through the ceramic discs (p < .004). The color change of both cements was lower through the ceramic veneer and this reduction was statistically significant (p < .001). CONCLUSION: Noticeable color change may be expected in areas of cement exposure for both cements studied. Also, in case of using Variolink cement, the color change may be seen through the ceramic veneers.

Effect of aging on color stability of amine-free resin cement through the ceramic laminate veneer.

BACKGROUND: This study aimed to assess the color change of two amine-free dual-cure resin cements following aging in comparison with a light-cure resin cement. MATERIALS AND METHODS: This in vitro, experimental study evaluated 6 groups (n = 10), including three groups of cements bonded to feldspathic porcelain and three groups of resin cements alone. Panavia V5 (Kuraray) and G-CEM LinkForce (GC) dual-cure resin cements and Choice 2 (Bisco) light-cure resin cements were bonded to porcelain according to the manufacturers' instructions. The color parameters of the groups were measured before and after 5000 thermal cycles by spectrophotometry. Data were analyzed using two-way ANOVA and Tukey's post hoc test (α =0.05). RESULTS: The color change (ΔE) of the cement groups alone was significantly greater than that of porcelain-bonded cements. The ΔE of G-CEM LinkForce cement group was significantly higher than that of Panavia V5 group (P = 0.020), and the ΔE of the latter group was significantly higher than that of Choice 2 cement group (P = 0.021). CONCLUSION: Considering the color change of cements evaluated in this study, Choice 2 and Panavia V5 cements bonded to porcelain showed acceptable color change, which indicates that they are suitable for cementation of esthetic dental restorations. Ceramic laminates have become an ideal esthetic treatment for anterior restorations. The resin cement discoloration can affect final appearance of these restorations. Besides that, exposed resin cement line can lead to the esthetic problem over time.