1.
Characterization of high molecular weight impurities in synthetic phosphorothioate oligonucleotides.
Bioorg Med Chem Lett
; 16(3): 607-14, 2006 Feb.
Artículo
en Inglés
| MEDLINE
| ID: mdl-16274991
RESUMEN
Phosphorothioate oligonucleotides manufactured by standard phosphoramidite techniques using 2'-deoxyadenosine- or 2'-O-(2-methoxyethyl)-5-methylcytosine-loaded solid supports contain branched impurities consisting of two chains linked through the exocyclic amino group of the 3'-terminal nucleoside of one chain and the 3'-terminal hydroxyl group of another via a P(O)SH group. These impurities are not produced when a universal, non-nucleoside derivatized support is used.
Asunto(s)
Oligonucleótidos/síntesis química , Compuestos Organofosforados/química , Tionucleótidos/química , 5-Metilcitosina/química , Cromatografía Líquida de Alta Presión , Desoxiadenosinas/química , Estructura Molecular , Peso Molecular , Espectrofotometría Ultravioleta
2.
Nucleosides Nucleotides Nucleic Acids
; 23(1-2): 149-60, 2004.
Artículo
en Inglés
| MEDLINE
| ID: mdl-15043143
RESUMEN
Multiple phosphorothioate oligonucleotides containing a 3'-terminal negative charge were synthesized and characterized. Influence of the added negative charge on activation of duplexes by RNase H was investigated. No additional help in recruitment of RNase H was observed.