RESUMEN
Senecavirus A (SVA) is a non-enveloped, single-stranded, positive-sense RNA virus belonging to the Picornaviridae family. Senecavirus A is constantly associated with outbreaks of vesicular disease in pigs and has been reported in several countries since its first large-scale outbreak in 2014. Senecavirus A's clinical disease and lesions are indistinguishable from other vesicular foreign animal diseases (FAD). Therefore, an FAD investigation needs to be conducted for every SVA case. For this reason, SVA has been attributed as the cause of an alarming increase in the number of yearly FAD investigations performed by the United States Department of Agriculture (USDA). The objectives of this study were to estimate the seroprevalence of SVA antibodies in breeding and growing pig farms in the United States and to determine the farm-level risk factors associated with seropositivity. A total of 5,794 blood samples were collected from 98 and 95 breeding and growing pig farms in 17 states. A farm characteristics questionnaire was sent to all farms, to which 80% responded. The responses were used to conduct logistic regression analyses to assess the risk factors associated with SVA seropositivity. The estimated farm-level seroprevalences were 17.3% and 7.4% in breeding and growing pig farms, respectively. Breeding farms had 2.64 times higher odds of SVA seropositivity than growing pig farms. One key risk factor identified in breeding farms was the practice of rendering dead animal carcasses. However, the adoption of a higher number of farm biosecurity measures was associated with a protective effect against SVA seropositivity in breeding farms.
RESUMEN
Eosinophilic myocarditis is a human condition that has been rarely documented in animals. We now report two unrelated porcine cases of idiopathic eosinophilic granulomatous myocarditis that resembled the human disease and which were associated with sudden death. The most relevant gross finding in both cases was marked cardiomegaly, accompanied by raised, multifocal to coalescent small white nodules (1-2 mm) and poorly demarcated multifocal pale areas in the epicardium. Histologically, there were multifocal to coalescent areas of cardiomyocyte loss with replacement by an intense inflammatory infiltrate of eosinophils and epithelioid macrophages, and proliferation of fibrous connective tissue. Immunohistochemistry for porcine circovirus type 2 (PCV2) and Toxoplasma gondii, in-situ hybridization and quantitative polymerase chain reaction tests for PCV2 and porcine circovirus type 3 and aerobic bacterial culture on myocardium samples were negative.
Asunto(s)
Infecciones por Circoviridae , Circovirus , Miocarditis , Enfermedades de los Porcinos , Animales , Infecciones por Circoviridae/veterinaria , ADN Viral/análisis , Humanos , Hibridación in Situ/veterinaria , Miocarditis/complicaciones , Miocarditis/veterinaria , Porcinos , Enfermedades de los Porcinos/patologíaRESUMEN
Senecavirus A (SVA) infection in pigs causes vesicular disease and results in a short viremia and transient shedding of the virus, mainly in oral fluids and feces. Here we describe the consistent prolonged shedding of SVA in the semen of 2 boars, and persistence of SVA within the tonsils and testes of 3 adult boars. Two SVA-infected boars that were identified on a Minnesota sow farm in 2017 shed SVA RNA in semen for >3 mo after an outbreak of vesicular disease had occurred on the farm. SVA was isolated from 1 semen sample collected 9 d after clinical disease began on the farm. The third SVA-infected boar was identified on an Indiana sow farm in 2020. All boars had SVA RNA detected in the testes and tonsils by RT-rtPCR, with lower Ct values obtained for the testes than from the tonsils. All boars had multifocal lymphocytic orchitis with segmental degeneration and atrophy of the germinal epithelium within the seminiferous tubules. One boar also had areas of seminiferous tubule collapse and interstitial fibrosis within the testes. In all boars, in situ hybridization demonstrated the presence of SVA mRNA within cells located basally in the seminiferous tubules of the testes, and within the basal surface epithelial cells, crypt epithelial cells, and subepithelial and parafollicular lymphocytes and histiocytes of the tonsil.
Asunto(s)
Picornaviridae , Enfermedades de los Porcinos , Animales , Femenino , Masculino , Picornaviridae/genética , ARN , Porcinos , Enfermedades de los Porcinos/epidemiología , Viremia/veterinariaRESUMEN
The enteric pathogen Lawsonia intracellularis is one of the main causes of diarrhea and compromised weight gain in pigs worldwide. Traditional cell-line cultures have been used to study L. intracellularis pathogenesis. However, these systems fail to reproduce the epithelial changes observed in the intestines of L. intracellularis-infected pigs, specifically, the changes in intestinal cell constitution and gene expression. A more physiologically accurate and state-of-the-art model is provided by swine enteroids derived from stem cell-containing crypts from healthy pigs. The objective of this study was to verify the feasibility of two-dimensional swine enteroids as in vitro models for L. intracellularis infection. We established both three- and two-dimensional swine enteroid cultures derived from intestinal crypts. The two-dimensional swine enteroids were infected by L. intracellularis in four independent experiments. Enteroid-infected samples were collected 3 and 7 d postinfection for analysis using real-time quantitative PCR and L. intracellularis immunohistochemistry. In this study, we show that L. intracellularis is capable of infecting and replicating intracellularly in two-dimensional swine enteroids derived from ileum.
Asunto(s)
Infecciones por Desulfovibrionaceae/veterinaria , Lawsonia (Bacteria) , Organoides/metabolismo , Enfermedades de los Porcinos/microbiología , Animales , Infecciones por Desulfovibrionaceae/microbiología , Infecciones por Desulfovibrionaceae/patología , Inmunohistoquímica , Mucosa Intestinal/patología , Intestinos/patología , Porcinos , Enfermedades de los Porcinos/patologíaRESUMEN
Senecavirus A (SVA) is an emerging picornavirus that causes vesicular disease (VD) in swine. The virus has been circulating in swine in the United Stated (USA) since at least 1988, however, since 2014 a marked increase in the number of SVA outbreaks has been observed in swine worldwide. The factors that led to the emergence of SVA remain unknown. Evolutionary changes that accumulated in the SVA genome over the years may have contributed to the recent increase in disease incidence. Here we compared full-genome sequences of historical SVA strains (identified before 2010) from the USA and global contemporary SVA strains (identified after 2011). The results from the genetic analysis revealed 6.32â% genetic divergence between historical and contemporary SVA isolates. Selection pressure analysis revealed that the SVA polyprotein is undergoing selection, with four amino acid (aa) residues located in the VP1 (aa 735), 2A (aa 941), 3C (aa 1547) and 3D (aa 1850) coding regions being under positive/diversifying selection. Several aa substitutions were observed in the structural proteins (VP1, VP2 and VP3) of contemporary SVA isolates when compared to historical SVA strains. Some of these aa substitutions led to changes in the surface electrostatic potential of the structural proteins. This work provides important insights into the molecular evolution and epidemiology of SVA.
Asunto(s)
Enfermedades Transmisibles Emergentes , Infecciones por Picornaviridae/veterinaria , Picornaviridae/genética , Enfermedades de los Porcinos/virología , Sustitución de Aminoácidos/genética , Animales , Enfermedades Transmisibles Emergentes/veterinaria , Enfermedades Transmisibles Emergentes/virología , Brotes de Enfermedades , Evolución Molecular , Variación Genética , Genoma Viral , Filogenia , Infecciones por Picornaviridae/epidemiología , Porcinos , Enfermedades de los Porcinos/epidemiología , Estados Unidos/epidemiología , Proteínas Virales/genética , Proteínas Estructurales Virales/genéticaRESUMEN
Next generation sequencing (NGS) can be applied to identify and characterize the entire set of microbes within a sample. However, this platform does not provide a morphological context or specific association between the viral or bacterial sequences detected and the histological lesions. This limitation has generated uncertainty whether the sequences identified by NGS are actually contributing or not for the clinical outcome. Although in situ hybridization (ISH) and immunohistochemistry (IHC) can be used to detect pathogens in tissue samples, only ISH has the advantage of being rapidly developed in a context of an emerging disease, especially because it does not require development of specific primary antibodies against the target pathogen. Based on the sequence information provided by NGS, ISH is able to check the presence of a certain pathogen within histological lesions, by targeting its specific messenger RNA, helping to build the relationship between the pathogen and the clinical outcome. In this mini review we have compiled results of the application of NGS-ISH to the investigation of challenging diagnostic cases or emerging pathogens in pigs, that resulted in the detection of porcine circovirus type 3, porcine parvovirus type 2, Senecavirus A, and Mycoplasma hyorhinis.
RESUMEN
Lawsonia intracellularis, an obligate intracellular bacterium, is an important enteric pathogen in pig herds and horse farms worldwide. The hallmark feature of L. intracellularis infection is the proliferation of epithelial cells in intestinal crypts. A major limitation to the study of L. intracellularis infection is the lack of an in vitro model that reproduces the changes observed in proliferative enteropathy. Here we investigated the suitability of mouse enteroids as a model to study L. intracellularis infection. Mouse enteroids were microinjected with L. intracellularis, filter-sterilized L. intracellularis culture supernatant, or sterile cell culture media (DMEM). L. intracellularis antigen was detected in mouse enteroids by immunohistochemistry and was located mostly in the basal region of the epithelium. There was no differential growth of enteroids among treatment groups, and cellular proliferation was not increased in L. intracellularis-infected enteroids in relation to non-infected enteroids based on immunofluorescence staining. L. intracellularis infection did not induce changes in gene expression of Ki-67 (proliferation marker), Sox9 (marker for transit amplifying cells) and Muc2 (marker for goblet cells). These results indicate that although L. intracellularis antigen is detectable in mouse enteroids, indicating susceptibility to infection, mouse enteroids fail to replicate the cellular proliferation and gene expression changes observed in proliferative enteropathy. Nevertheless, we have successfully demonstrated that mouse enteroids can be used to model days-long intracellular pathogen infection, serving as potential models for the study of other pathogens of interest in veterinary medicine.
Asunto(s)
Infecciones por Desulfovibrionaceae/veterinaria , Enfermedades Intestinales/veterinaria , Lawsonia (Bacteria)/fisiología , Organoides/microbiología , Enfermedades de los Porcinos/microbiología , Animales , Infecciones por Desulfovibrionaceae/microbiología , Modelos Animales de Enfermedad , Humanos , Ratones , PorcinosRESUMEN
Conjunctivitis is an uncommon finding in commercial swine herds, and the etiology of the disease is rarely studied. We investigated cases of conjunctivitis in 3 wean-to-finish swine farms. Eye swabs and tissues were obtained from clinically affected pigs (8-22 wk of age), from unaffected pigs in contact with affected pen-mates, and from age-matched pigs from an unaffected herd. Real-time PCR (rtPCR) testing for Mycoplasma hyorhinis demonstrated consistent detection and high bacterial load in samples from affected herds (clinically affected animals and non-clinical pen-mates). Ct values in affected pigs were 18.9-25.3; values were 36.4-38.6 in unaffected pigs from unaffected herds. Additionally, M. hyorhinis was identified within inflamed palpebral conjunctivae by in situ hybridization. The association of rtPCR and in situ detection of M. hyorhinis, along with the lack of detection of other potential pathogens and noninfectious causes, suggests the involvement of M. hyorhinis in the etiology and pathogenesis of the reported swine conjunctivitis.
Asunto(s)
Conjuntivitis/veterinaria , Brotes de Enfermedades/veterinaria , Infecciones por Mycoplasma/veterinaria , Mycoplasma hyorhinis/aislamiento & purificación , Enfermedades de los Porcinos/epidemiología , Animales , Conjuntivitis/epidemiología , Conjuntivitis/microbiología , Hibridación in Situ/veterinaria , Minnesota/epidemiología , Infecciones por Mycoplasma/epidemiología , Infecciones por Mycoplasma/microbiología , Ohio/epidemiología , Oklahoma/epidemiología , Reacción en Cadena en Tiempo Real de la Polimerasa/veterinaria , Porcinos , Enfermedades de los Porcinos/microbiologíaRESUMEN
Lawsonia intracellularis is an obligate intracellular bacterium that causes proliferative enteropathy in various animal species. While cellular proliferation of intestinal cells is recognized as the hallmark of L. intracellularis infection in vivo, it has not been demonstrated in in vitro models. In order to assay the effect of L. intracellularis, various cell lines were infected with pathogenic and non-pathogenic passages of the bacterium. Because of the high proliferative rate of these cell lines, serum deprivation, which is known to reduce proliferation, was applied to each of the cell lines to allow the observation of proliferation induced by L. intracellularis. Using antibodies for Ki-67 and L. intracellularis in dual immunofluorescence staining, we observed that L. intracellularis was more frequently observed in proliferating cells. Based on wound closure assays and on the amount of eukaryotic DNA content measured over time, we found no indication that cell lines infected with L. intracellularis increased proliferation and migration when compared to non-infected cells (p > 0.05). Cell arrest due to decreased serum in the culture media was cell-line dependent. Taken together, our findings provide data to support and expand previous subjective observations of the absence of in vitro proliferation caused by L. intracellularis in cell cultures and confirm that cell lines infected by L. intracellularis fail to serve as adequate models for understanding the cellular changes observed in proliferative enteropathy-affected intestines.
Asunto(s)
Infecciones por Desulfovibrionaceae/microbiología , Enfermedades Intestinales/veterinaria , Lawsonia (Bacteria)/inmunología , Animales , Línea Celular , Proliferación Celular , Células Epiteliales/microbiología , Enfermedades Intestinales/microbiología , Intestinos/microbiología , MamíferosRESUMEN
Rotavirus groups A, B, and C (RVA, RVB, and RVC, respectively) have been the most prevalent and pathogenic in pigs. To date, immunohistochemistry is only available for RVA because of the lack of commercial antibodies for RVB and RVC. We developed a novel in situ hybridization RNA-based chromogenic technique (ISH-RNA) to detect and subtype RVA, RVB, and RVC. We evaluated 33 samples that were reverse-transcription PCR positive for RVA, RVB, and/or RVC. ISH-RNA was able to detect as few as 103 RV RNA copies/mL. The new ISH-RNA test can be useful for routine investigation of rotavirus enteritis in order to guide strategies for control of the infection in pigs, but a full validation study needs to be completed. Pathogenesis studies may be conducted using ISH-RNA based on the identification of replicating virus.
Asunto(s)
Hibridación in Situ/veterinaria , Infecciones por Rotavirus/veterinaria , Rotavirus/aislamiento & purificación , Enfermedades de los Porcinos/diagnóstico , Animales , Hibridación in Situ/métodos , Infecciones por Rotavirus/diagnóstico , Infecciones por Rotavirus/virología , Sus scrofa , Porcinos , Enfermedades de los Porcinos/virologíaRESUMEN
Lawsonia intracellularis is among the most important enteric pathogens of swine and antibiotic alternatives are needed to help mitigate the negative effects of infection. Zinc is an essential trace mineral known to be crucial for maintaining intestinal barrier function and proper immune response. In this study, we investigated the porcine host response to L. intracellularis infection when supplemented with a zinc-amino acid complex, a form of zinc that can lead to greater bioavailability when compared to traditional inorganic forms of zinc. Our results show that a zinc-amino acid complex supplementation with a final concentration of 125 ppm of zinc in feed significantly (p < 0.05) decreased the number of animals with lesions and severity of lesions caused by L. intracellularis. Animals supplemented with the zinc-amino acid complex also exhibited a significantly (p < 0.05) earlier onset of seroconversion as well as an increased number of T cells in infected and non-infected intestinal tissue. This study demonstrated that this zinc-amino acid complex aids the host in responding to L. intracellularis infection and may be a new approach to help minimize negative effects of disease.
Asunto(s)
Aminoácidos/metabolismo , Infecciones por Desulfovibrionaceae/inmunología , Lawsonia (Bacteria)/fisiología , Sus scrofa/inmunología , Enfermedades de los Porcinos/inmunología , Zinc/metabolismo , Aminoácidos/administración & dosificación , Alimentación Animal/análisis , Animales , Dieta/veterinaria , Suplementos Dietéticos/análisis , Agua Potable/análisis , Femenino , Masculino , Porcinos , Zinc/administración & dosificaciónRESUMEN
Mycoplasmal pneumonia is an important disease in the pig industry. Due to the controversial role of Mycoplasma hyorhinis in this disease, confirmation of the presence of this bacterium and the identification of its roles in respiratory disease remain major challenges. The objectives of this study were to evaluate the presence of M. hyorhinis in early cases of mycoplasmal pneumonia and to determine the usefulness of fluorescent in situ hybridization (FISH) for the diagnosis of respiratory mycoplasmosis in naturally infected pigs. Ninety M. hyopneumoniae and/or M. hyorhinis-infected lung tissue samples based on diagnostic mosaic (DM) were used. The average age of the animals was 116 and 57 days (P<0.01) for groups 1 (positive-M. hyopneumoniae only) and 2 (positive-M. hyorhinis only), respectively. These findings suggest that development of lesions caused by M. hyorhinis occurs earlier than for M. hyopneumoniae. Using the DM as the gold standard, the sensitivity and specificity of FISH for M. hyopneumoniae were 75 and 100%, respectively, and were 40 and 73.3% for the immunohistochemistry (IHC). The sensitivity and specificity of FISH for M. hyorhinis were 76.7 and 100%, respectively. These findings demonstrate that FISH can be a useful tool for diagnosing mycoplasmosis. Viral antigens (PCV2 or influenza A) were detected in 53.3% (16/30) of the samples in group 2 (M. hyorhinis-PCR positive) and 13.3% (4/30) of the samples in group 1 (M. hyopneumoniae-PCR positive). This finding indicates that the association of M. hyorhinis and viral infection in nursery pigs likely starts due to a viral immunosuppressive condition.(AU)
A pneumonia micoplásmica causada por bactérias do gênero Mycoplasma é uma enfermidade de grande importância para indústria suinícola, sendo ainda controverso o papel desempenhado por Mycoplasma hyorhinis nessa doença. A confirmação da presença dessas bactérias bem como a identificação de seus papéis em doenças respiratórias continua sendo um grande desafio. Os objetivos desse estudo foram comparar diferentes técnicas, em especial a de hibridização fluorescente in situ (FISH), para diagnóstico de micoplasmoses respiratória em suínos naturalmente infectados e avaliar a presença do M. hyorhinis em casos precoces de pneumonia micoplásmica. Foram utilizadas 90 amostras de tecido pulmonar infectado para cada um ou ambos os agentes (M. hyopneumoniae e M. hyorhinis) determinados pelo mosaico de diagnóstico (sinais clínicos, lesões macroscópicas e microscópicas e pela PCR). No grupo de animais positivos pela PCR apenas para M. hyorhinis (Grupo 2) a média da idade foi de 57,32 dias e no grupo apenas positivo para M. hyopneumoniae (Grupo 1) a média foi de 116,31 dias (P<0,01). Estes achados sugerem que a colonização e o aparecimento de lesões causadas pelo M. hyorhinis seja mais precoce do que aquelas causadas pelo M. hyopneumoniae. As alterações microscópicas foram estatisticamente (P<0,01) mais intensas no grupo 1 do que no grupo 2. Usando o mosaico de diagnóstico como padrão ouro, a sensibilidade e especificidade na FISH para M. hyopneumoniae foi de 75 e 100%, respectivamente, e 40 e 73,3%, na imuno-histoquímica. A sensibilidade e especificidade da FISH para M. hyorhinis foi de 76,7 e 100%. Esses valores demonstram que a FISH pode ser uma ferramenta útil para diagnóstico de micoplasmoses. Foi detectada a presença de agentes virais (PCV2 ou influenza) em 53,3% das amostras do grupo 2 (M. hyorhinis) e em 13,3% das amostras do grupo 1 (M. hyopneumoniae).(AU)
Asunto(s)
Animales , Sus scrofa/microbiología , Mycoplasma hyopneumoniae , Mycoplasma hyorhinis , Neumonía Porcina por Mycoplasma/diagnóstico , Infecciones por Mycoplasma/diagnóstico , Infecciones por Mycoplasma/veterinaria , Hibridación Fluorescente in Situ/veterinariaRESUMEN
Seneca Valley virus (SVV) is the causative agent of an emerging vesicular disease in swine, which is clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. In addition, SVV has been associated with neonatal mortality in piglets. While a commercial SVV qRT-PCR is available, commercial antibodies are lacking to diagnose SVV infections by immunohistochemistry (IHC). Thus, a novel in situ hybridization technique-RNAscope (ISH) was developed to detect SVVRNA in infected tissues. From a total of 78 samples evaluated, 30 were positive by qRT-PCR and ISH-RNA, including vesicular lesions of affected sows, ulcerative lesions in the tongue of piglets and various other tissues with no evidence of histological lesions. Nineteen samples were negative for SVV by qRT-PCR and ISH-RNA. The Ct values of the qRT-PCR from ISH-RNA positive tissues varied from 12.0 to 32.6 (5.12 x 106 to 5.31 RNA copies/g, respectively). The ISH-RNA technique is an important tool in diagnosing and investigating the pathogenesis of SVV and other emerging pathogens.
Asunto(s)
Infecciones por Picornaviridae/veterinaria , Picornaviridae , Enfermedades de los Porcinos/diagnóstico , Enfermedades de los Porcinos/virología , Animales , Animales Recién Nacidos , Femenino , Corazón/virología , Inmunohistoquímica , Hibridación in Situ , Ganglios Linfáticos/metabolismo , Ganglios Linfáticos/patología , Ganglios Linfáticos/virología , Miocardio/metabolismo , Miocardio/patología , Necrosis/metabolismo , Necrosis/patología , Necrosis/virología , Senos Paranasales/metabolismo , Senos Paranasales/patología , Senos Paranasales/virología , Infecciones por Picornaviridae/diagnóstico , Infecciones por Picornaviridae/metabolismo , Infecciones por Picornaviridae/patología , ARN Viral/metabolismo , Reacción en Cadena en Tiempo Real de la Polimerasa , Sensibilidad y Especificidad , Bazo/metabolismo , Bazo/patología , Bazo/virología , Porcinos , Enfermedades de los Porcinos/metabolismo , Enfermedades de los Porcinos/patología , Lengua/metabolismo , Lengua/patología , Lengua/virologíaRESUMEN
The objectives of this study were to characterize Brachyspira hyodysenteriae isolates and to evaluate the antimicrobial susceptibility patterns of strains obtained from pigs in Brazil based on the minimal inhibitory concentration test (MIC). The MIC was performed for 22 B. hyodysenteriae isolates obtained from 2011 to 2013 using the following antimicrobial drugs: tylosin, tiamulin, valnemulin, doxycycline, lincomycin and tylvalosin. Outbreaks of swine dysentery were diagnosed based on clinical presentation, bacterial isolation, gross and microscopic lesions, duplex PCR for B. hyodysenteriae and B. pilosicoli and nox gene sequencing. All obtained MIC values were consistently higher or equal to the microbiological cut-off described in the literature. The MIC 90 values for the tested drugs were 8µg/ml for doxycycline, >4µg/ml for valnemulin, 8µg/ml for tiamulin, 32µg/ml for tylvalosin, >64µg/ml for lincomycin and >128µg/ml for tylosin. These results largely corroborate those reported in the literature. Tiamulin, doxycycline and tylvalosin showed the lowest MIC results. All of the samples subjected to phylogenetic analysis based on the nox gene sequence exhibited similar results, showing 100% identity to B. hyodysenteriae. This is the first study describing the MIC pattern of B. hyodysenteriae isolated in Brazil.(AU)
Os objetivos deste trabalho foram a caracterização de isolados de Brachyspira hyodysenteriae e avaliar os padrões de sensibilidade antimicrobiana de isolados obtidos a partir de suínos no Brasil com base no teste de concentração inibitória mínima (MIC). A MIC foi realizada em 22 isolados de B. hyodysenteriae obtidos entre 2011 a 2013 usando os seguintes antimicrobianos: tilosina, tiamulina, valnemulina, doxiciclina, lincomicina e tilvalosina. Surtos de disenteria suína foram diagnosticados com base na apresentação clínica, isolamento bacteriano, lesões macroscópicas e microscópicas, PCR duplex para B. hyodysenteriae e B. pilosicoli e sequenciamento do gene nox. Todos os valores de MIC obtidos foram consistentemente mais elevados ou igual ao ponto de corte microbiológica descrito na literatura. Os valores de MIC 90 para os fármacos testados foram de 8 µg / mL para a doxiciclina, > 4 µg/ml de valnemulina, 8 µg / mL para a tiamulina, 32 µg / ml para tilvalosina, > 64 µg / ml para a lincomicina e > 128 µg / ml de tilosina. Estes resultados corroboram em grande parte com os relatados na literatura. Tiamulina, doxiciclina e tilvalosina apresentaram os menores resultados de MIC. Todas as amostras submetidas à análise filogenética com base na sequência do gene nox exibiram resultados semelhantes, indicando 100% de identidade com B. hyodysenteriae. Este é o primeiro estudo que descreve o padrão MIC de B. hyodysenteriae isoladas no Brasil.(AU)
Asunto(s)
Pruebas de Sensibilidad Microbiana/veterinaria , Brachyspira hyodysenteriae/aislamiento & purificación , NADPH Oxidasas , Reacción en Cadena de la Polimerasa/veterinaria , Disentería/veterinariaRESUMEN
BACKGROUND: Senecavirus A (SVA), a member of the family Picornaviridae, genus Senecavirus, is a recently identified single-stranded RNA virus closely related to members of the Cardiovirus genus. SVA was originally identified as a cell culture contaminant and was not associated with disease until 2007 when it was first observed in pigs with Idiopathic Vesicular Disease (IVD). Vesicular disease is sporadically observed in swine, is not debilitating, but is significant due to its resemblance to foreign animal diseases, such as foot-and-mouth disease (FMD), whose presence would be economically devastating to the United States. IVD disrupts swine production until foreign animal diseases can be ruled out. Identification and characterization of SVA as a cause of IVD will help to quickly rule out infection by foreign animal diseases. METHODS: We have developed and characterized an indirect ELISA assay to specifically identify serum antibodies to SVA. Viral protein 1, 2 and 3 (VP1, VP2, VP3) were expressed, isolated, and purified from E. coli and used to coat plates for an indirect ELISA. Sera from pigs with and without IVD symptoms as well as a time course following animals from an infected farm, were analyzed to determine the antibody responses to VP1, VP2, and VP3. RESULTS: Antibody responses to VP2 were higher than VP1 and VP3 and showed high affinity binding on an avidity ELISA. ROC analysis of the SVA VP2 ELISA showed a sensitivity of 94.2% and a specificity of 89.7%. Compared to IFA, the quantitative ELISA showed an 89% agreement in negative samples and positive samples from 4-60 days after appearance of clinical signs. Immune sera positive for FMDV, encephalomyocarditis virus, and porcine epidemic diarrhea virus antibodies did not cross-react. CONCLUSIONS: A simple ELISA based on detection of antibodies to SVA VP2 will help to differentially diagnose IVD due to SVA and rule out the presence of economically devastating foreign animal diseases.
Asunto(s)
Anticuerpos Antivirales/sangre , Ensayo de Inmunoadsorción Enzimática/veterinaria , Infecciones por Picornaviridae/veterinaria , Picornaviridae/inmunología , Enfermedades de los Porcinos/virología , Animales , Clonación Molecular , Ensayo de Inmunoadsorción Enzimática/métodos , Escherichia coli/genética , Escherichia coli/metabolismo , Técnica del Anticuerpo Fluorescente Indirecta/veterinaria , Regulación Viral de la Expresión Génica , Picornaviridae/aislamiento & purificación , Infecciones por Picornaviridae/diagnóstico , Infecciones por Picornaviridae/virología , Sensibilidad y Especificidad , Porcinos , Enfermedades de los Porcinos/diagnóstico , Proteínas Virales/genética , Proteínas Virales/inmunología , Proteínas Virales/metabolismoRESUMEN
Senecavirus A (SVA) is an emerging picornavirus that has been associated with vesicular disease and neonatal mortality in swine. Many aspects of SVA infection biology and pathogenesis, however, remain unknown. Here the pathogenesis of SVA was investigated in finishing pigs. Animals were inoculated via the oronasal route with SVA strain SD15-26 and monitored for clinical signs and lesions associated with SVA infection. Viraemia was assessed in serum and virus shedding monitored in oral and nasal secretions and faeces by real-time reverse transcriptase quantitative PCR (RT-qPCR) and/or virus isolation. Additionally, viral load and tissue distribution were assessed during acute infection and following convalescence from disease. Clinical signs characterized by lethargy and lameness were first observed on day 4 post-inoculation (pi) and persisted for approximately 2-10 days. Vesicular lesions were first observed on day 4 pi on the snout and/or feet, affecting the coronary bands, dewclaws, interdigital space and heel/sole of SVA-infected animals. A short-term viraemia was observed between days 3 and 10 pi, whereas virus shedding was detected between days 1 and 28 pi in oral and nasal secretions and faeces. Notably, RT-qPCR and in situ hybridization (ISH) performed on tissues collected on day 38 pi revealed the presence of SVA RNA in the tonsils of all SVA-infected animals. Serological responses to SVA were characterized by early neutralizing antibody responses (day 5 pi), which coincided with decreased levels of viraemia, virus shedding and viral load in tissues. This study provides significant insights into the pathogenesis and infectious dynamics of SVA in swine.
Asunto(s)
Picornaviridae/patogenicidad , Enfermedades de los Porcinos/virología , Viremia/veterinaria , Animales , Anticuerpos Neutralizantes/sangre , Anticuerpos Antivirales/sangre , Picornaviridae/genética , Picornaviridae/aislamiento & purificación , Picornaviridae/fisiología , Porcinos , Enfermedades de los Porcinos/sangre , Enfermedades de los Porcinos/patología , Carga Viral , Viremia/sangre , Viremia/patología , Viremia/virología , Virulencia , Esparcimiento de VirusRESUMEN
Nodular lung lesions in swine are frequently due to abscesses or granulomatous pneumonia. Although tumours are rarely reported in modern pig farming, they should be considered as a differential diagnosis when nodular lung lesions are found. A first-parity sow exhibiting respiratory signs was euthanized. Several whitish firm nodules, not encapsulated, ranging in diameter from 0.5 to 5 cm were present in all lung lobes. Microscopically, the nodules were composed of dense neoplastic cells, mainly in Antoni types A and B patterns, infiltrative and with development of emboli. All neoplastic cells stained positively by immunohistochemistry for vimentin and S-100 protein, with variable immunostaining for glial fibrillary acidic protein and stained negative for cytokeratin. Based on the gross, histological and immunohistochemical features, the tumor was diagnosed as malignant peripheral nerve sheath tumour.
Asunto(s)
Neoplasias Pulmonares/veterinaria , Neurilemoma/veterinaria , Enfermedades de los Porcinos/diagnóstico , Animales , Diagnóstico Diferencial , Femenino , Inmunohistoquímica/veterinaria , Neoplasias Pulmonares/diagnóstico , Neoplasias Pulmonares/patología , Neurilemoma/diagnóstico , Neurilemoma/patología , Proteínas S100/química , Porcinos , Enfermedades de los Porcinos/patología , Vimentina/químicaRESUMEN
Lawsonia intracellularis infection causes proliferative enteropathy (PE) in many mammalian species, with porcine and equine proliferative enteropathy (PPE and EPE) known worldwide. Hamsters are a well-published animal model for PPE infection studies in pigs. There is no laboratory animal model for EPE infection studies and it is not known whether there is species-specificity for equine or porcine isolates of L. intracellularis in animal models. The objective of this study was to determine whether it is possible to generate typical EPE lesions in hamsters after inoculation with an equine strain of L. intracellularis (EPE strain) and whether it is comparatively possible to generate PPE lesions in rabbits after inoculation with a porcine strain of L. intracellularis (PPE strain). In 2 separate trials, 4-week-old and 3-week-old weanling golden Syrian hamsters were challenged with EPE strains and compared to uninfected (both trials) and PPE-infected controls (Trial 2 only). Concurrently, 6 female New Zealand white juvenile rabbits were infected with PPE strain and observed concomitantly to 8 similar rabbits infected with EPE strain for a different experiment. Hamsters and rabbits were observed for 21 to 24 days post-infection (DPI), depending on the experiment. Neither infected species developed clinical signs. The presence of disease was assessed with diagnostic techniques classically used for pigs and horses: immune-peroxidase monolayer assay on sera; quantitative polymerase chain reaction (qPCR) detection of molecular DNA in feces; and hematoxylin and eosin (H&E) stain and immunohistochemistry (IHC) on intestinal tissues. Our results showed that EPE-challenged hamsters do not develop infection when compared with PPE controls (IHC, P = 0.009; qPCR, P = 0.0003). Conversely, PPE-challenged rabbits do not develop typical intestinal lesions in comparison to EPE-challenged rabbits, with serological response at 14 DPI being significantly lower (P = 0.0023). In conclusion, PPE and EPE strains appear to have different host-specificities for hamsters and rabbits, respectively.
L'infection par Lawsonia intracellularis provoque une entéropathie proliférative chez de nombreuses espèces de mammifères; celle des porcins (EPP) et des équidés (EEP) sont connues mondialement. Les hamsters sont un modèle animal bien connu pour l'étude de l'EPP. Il n'existe pas de modèle animal de laboratoire pour étudier l'EEP, et on ne sait pas s'il y a spécificité d'espèce pour les isolats équins ou porcins de L. intracellularis dans des modèles animaux. L'objectif de la présente étude était de déterminer s'il est possible de générer des lésions typiques d'EEP chez les hamsters après inoculation d'une souche équine de L. intracellularis (souche EEP) et s'il est également possible de générer des lésions d'EPP chez des lapins après inoculation d'une souche porcine de L. intracellularis (souche EPP). Dans 2 essais séparés, des hamsters dorés syriens sevrés âgés de 4 semaines et de 3 semaines ont été inoculés avec des souches EEP, et ont été comparés à des témoins non infectés (les deux essais) et à des témoins infectés avec EPP (essai 2 seulement). Parallèlement, 6 jeunes lapines Nouvelle-Zélande ont été infectées par la souche EEP et observées de façon concomitante à 8 lapins similaires infectés par la souche EPP pour une expérience différente. Les hamsters et les lapins ont été observés pendant 21 à 24 jours après l'infection (JAI), en fonction de l'expérience. Aucune des espèces infectées n'a développé de signes cliniques. La présence de maladie a été évaluée par des techniques classiques de diagnostic utilisées pour les porcs et les chevaux : l'essai par immuno-peroxydase sur monocouche pour les sérums; la détection par réaction d'amplification en chaîne par la polymérase quantitative (qPCR) de l'ADN moléculaire dans les selles; la coloration hématoxyline-éosine et l'immunohistochimie (IHC) sur des tissus intestinaux. Nos résultats ont montré que les hamsters inoculés avec EEP ne développent pas d'infection comparativement aux EPP témoins (IHC P = 0,009; qPCR P = 0,0003). À l'inverse, les lapins inoculés avec EPP ne développent pas des lésions intestinales typiques comparativement aux lapins inoculés avec EEP, avec une réponse sérologique à 14 JAI significativement plus faible (P = 0,0023). En conclusion, les souches d'EPP et d'EEP semblent avoir des spécificités d'hôte différentes chez les hamsters et les lapins, respectivement.(Traduit par Dr. J.M. Dhillon).
Asunto(s)
Infecciones por Desulfovibrionaceae/veterinaria , Enfermedades de los Caballos/microbiología , Enfermedades Intestinales/veterinaria , Lawsonia (Bacteria)/aislamiento & purificación , Enfermedades de los Porcinos/microbiología , Animales , Cricetinae , ADN Bacteriano/química , ADN Bacteriano/genética , Infecciones por Desulfovibrionaceae/inmunología , Infecciones por Desulfovibrionaceae/microbiología , Modelos Animales de Enfermedad , Heces/microbiología , Femenino , Enfermedades de los Caballos/inmunología , Caballos , Inmunohistoquímica/veterinaria , Enfermedades Intestinales/inmunología , Enfermedades Intestinales/microbiología , Lawsonia (Bacteria)/genética , Mesocricetus , Reacción en Cadena de la Polimerasa/veterinaria , Conejos , Distribución Aleatoria , Especificidad de la Especie , Organismos Libres de Patógenos Específicos , Estadísticas no Paramétricas , Porcinos , Enfermedades de los Porcinos/inmunologíaRESUMEN
The objective of this study was to demonstrate the susceptibility of rabbits to Lawsonia intracellularis obtained from a case of clinical equine proliferative enteropathy (EPE). This is a preliminary step toward developing a rabbit infection model for studying pathogenesis and therapy of EPE in horses. Nine does were equally assigned to 3 groups. Animals in 2 groups (Group 1 and Group 2) were orally inoculated with different doses of cell-cultured L. intracellularis. Controls (Group 3) were sham-inoculated. Feces and blood were collected before the rabbits were infected and at 7, 14, and 21 days post-infection (DPI). Serum immunoglobulin G (IgG) titers were measured using an immunoperoxidase monolayer assay (IPMA) and fecal samples were analyzed with quantitative polymerase chain reaction (qPCR). A doe from each group was euthanized at 7, 14, and 21 DPI for collection and evaluation of intestinal samples. Tissues were stained by routine hematoxylin and eosin (H&E) method and immunohistochemistry (IHC) with L. intracellularis-specific mouse monoclonal antibody. At 14 DPI, serologic responses were detected in both infected groups, which maintained high titers through to 21 DPI. Lawsonia intracellularis DNA was detected in the feces of Group 2 on 7 DPI and in both infected groups on 14 DPI. Gross lesions were apparent in Group 1 and Group 2 on 14 DPI. Immunohistochemistry confirmed L. intracellularis antigen within cells of rabbits in Group 1 and Group 2 on 7, 14, and 21 DPI. No lesions, serologic response, shedding, or IHC labeling were found in Group 3 rabbits. This study describes an EPE rabbit model that simulates natural infection, as typical lesions, immune response, and fecal shedding were present.
Cette étude visait à démontrer la susceptibilité des lapins à Lawsonia intracellularis obtenu d'un cas clinique d'entéropathie proliférative équine (EPE). Ceci est une étape préliminaire dans le développement d'un modèle d'infection chez le lapin pour étudier la pathogénie et le traitement de l'EPE chez les chevaux. Neuf lapines ont été assignées également à 3 groupes. Les animaux dans deux groupes (Groupe 1 et Groupe 2) ont été inoculés oralement avec différentes doses de L. intracellularis cultivés sur cellules. Les témoins (Groupe 3) étaient faussement inoculés. Des fèces et du sang ont été prélevés avant que les lapins soient infectés et aux jours 7, 14 et 21 post-infection (DPI). Les titres sériques d'immunoglobulines G (IgG) ont été mesurés par une épreuve d'immunoperoxydase en monocouche (IPMA) et les échantillons de fèces ont été analysés par réaction quantitative d'amplification en chaîne par la polymérase (qPCR). Une lapine de chaque groupe a été euthanasiée 7, 14 et 21 DPI pour prélèvement et évaluation d'échantillons intestinaux. Les tissus étaient colorés à l'aide d'hématoxyline et éosine (H&E) et en immunohistochime (IHC) avec un anticorps monoclonal de souris spécifique à L. intracellularis. Au jour 14 post-infection, une réponse sérologique a été détectée chez les animaux des deux groupes infectés, et des titres élevés ont été maintenus jusqu'à 21 DPI. De l'ADN de L. intracellularis fut détecté dans les fèces du Groupe 2 au jour 7 PI et dans les 2 groupes infectés au jour 14 PI. Des lésions macroscopiques étaient apparentes dans le Groupe 1 et le Groupe 2 au jour 14 PI. L'immunohistochime a confirmé la présence d'antigène de L. intracellularis à l'intérieur des cellules de lapins dans les Groupes 1 et 2 aux jours 7, 14 et 21 PI. Aucune lésion, réponse sérologique, excrétion, ou marquage en IHC n'ont été trouvés chez les lapins du Groupe 3. La présente étude décrit un modèle lapin d'EPE qui imite l'infection naturelle, étant donné la présence de lésions typiques, de réponse immunitaire et d'excrétion fécale.(Traduit par Docteur Serge Messier).
Asunto(s)
Infecciones por Desulfovibrionaceae/veterinaria , Enteritis/veterinaria , Enfermedades de los Caballos/microbiología , Lawsonia (Bacteria) , Conejos , Animales , Enteritis/microbiología , Enteritis/patología , Heces/microbiología , Femenino , Caballos , Enfermedades del Yeyuno/microbiología , Enfermedades del Yeyuno/patología , Enfermedades del Yeyuno/veterinaria , Yeyuno/patología , Reacción en Cadena de la PolimerasaRESUMEN
Lawsonia intracellularis is an obligate intracellular bacterium and the causative agent of proliferative enteropathy (PE). The disease is endemic in pigs, emerging in horses and has also been reported in a variety of other animal species, including nonhuman primates. Comparing the whole genome sequences of a homologous porcine L. intracellularis isolate cultivated for 10 and 60 passages in vitro, we identified a 18-kb prophage-associated genomic island in the passage 10 (pathogenic variant) that was lost in the passage 60 (non-pathogenic variant). This chromosomal island comprises 15 genes downstream from the prophage DLP12 integrase gene. The prevalence of this genetic element was evaluated in 12 other L. intracellularis isolates and in 53 infected animals and was found to be conserved in all porcine isolates cultivated for up to 20 passages and was lost in isolates cultivated for more than 40 passages. Furthermore, the prophage region was also present in 26 fecal samples derived from pigs clinically affected with both acute and chronic forms of the disease. Nevertheless, equine L. intracellularis isolates evaluated did not harbor this genomic island regardless of the passage in vitro. Additionally, fecal samples from 21 clinically affected horses and four wild rabbits trapped in horse farms experiencing PE outbreaks did not show this prophage-associated island. Although the presence of this prophage-associated island was not essential for a virulent L. intracellularis phenotype, this genetic element was porcine isolate-specific and potentially contributed to the ecological specialization of this organism for the swine host.