Contamination of polychlorinated biphenyls in honey from the Brazilian state of Rio Grande do Sul.

Persistent organic pollutants are characterised by their chemical structure, environmental persistence and toxicity to human and wildlife populations. The production of these chemicals is regulated and restricted. However, they continue to be detected in the environment. In this study, the occurrence of 11 congeners of polychlorinated biphenyls (PCBs 28, 52, 77, 81, 101, 118, 126, 138, 153, 169, and 180) was investigated in 90 honey samples produced in the Brazilian state of Rio Grande do Sul. The samples were from different municipalities, production systems and floral origins. Extraction was performed using the modified QuEChERS method (Quick, Easy, Cheap, Effective, Rugged and Safe) followed by gas chromatography with micro-electron capture detector. The results showed the presence of four congeners (PCBs 28, 77, 81, 101) in 15 honey samples confirming the environmental contamination in Southern Brazil. Among the contaminated samples, no significant differences were identified regarding the production system and floral origin.

Analytical method validation to evaluate dithiocarbamates degradation in biobeds in South of Brazil.

In order to evaluate the efficiency of biobeds on DTC degradation, the aim of this study was to apply, optimize and validate a method to determine dithiocarbamate (mancozeb) in biobeds using gas chromatography-tandem mass spectrometry (GC-MS). The DTC pesticide mancozeb was hydrolysed in a tin (II) chloride solution at 1.5% in HCl (4 mol L-1), during 1 h in a water bath at 80 °C, and the CS2 formed was extracted in isooctane. After cooling, 1 mL of the organic layer was transferred to an auto sampler vial and analyzed by GC-MS. A complete validation study was performed and the following parameters were assessed: linearity of the analytical curve (r2), estimated method and instrument limits of detection and limits of quantification (LODm, LODi, LOQm and LOQi, respectively), accuracy (recovery%), precision (RSD%) and matrix effects. Recovery experiments were carried out with a standard spiking solution of the DTC pesticide thiram. Blank biobed (biomixture) samples were spiked at the three levels corresponding to the CS2 concentrations of 1, 3 and 5 mg kg-1, with seven replicates each (n = 7). The method presented satisfactory accuracy, with recoveries within the range of 89-96% and RSD ≤ 11%. The analytical curves were linear in the concentration range of 0.05-10 µg CS2 mL-1 (r2 > 0.9946). LODm and LOQm were 0.1 and 0.5 mg CS2 kg-1, respectively, and the calculated matrix effects were not significant (≤ 20%). The validated method was applied to 80 samples (biomixture), from sixteen different biobeds (collected at five sampling times) during fourteen months. Ten percent of samples presented CS2 concentration below the LOD (0.1 mg CS2 kg-1) and 49% of them showed results below the LOQ (0.5 mg CS2 kg-1), which demonstrates the biobeds capability to degrade DTC.