Antitumor Effect of Epigallocatechin Gallate and Vincristine in Mice with L5178Y Lymphoma.

The main objective of research into new therapies is the search for more efficacy and fewer toxic effects in cancer treatments. On one hand, vincristine (VCR) is a chemotherapeutic used in different kinds of tumors. On the other hand, epigallocatechin gallate (EGCG) is a green tea metabolite that has shown an antineoplastic effect in diverse investigations, so the objective of this work is to evaluate the antitumor effects of the EGCG/VCR combination on tumor volume and survival. To achieve this objective, the solid model of lymphoma L5178Y was used in BALB/c mice with different doses of VCR, EGCG, and their combination allowed tumor growth and survival time recording. After tumor collection, measurements, and immunohistochemistry for p53, Bcl2, and Cyclin D1 were performed. The results showed that the EGCG/vincristine combination had a greater antitumor effect than those effects of vincristine and EGCG. It can be attributed to the fact that the greatest inhibition of Bcl2 was present in gathering of EGCG harvest with vincristine. Therefore, the combination of EGCG with vincristine has a better antineoplastic effect by inhibiting tumor development and increasing survival on both substances independently.

Microaerobic condition as pretreatment for improving anaerobic digestion: A review.

Pretreatment of waste before anaerobic digestion (AD) has been extensively studied during the last decades. One of the biological pretreatments studied is the microaeration. This review examines this process, including parameters and applications to different substrates at the lab, pilot and industrial scales, to guide further improvement in large-scale applications. The underlying mechanisms of accelerating hydrolysis and its effects on microbial diversity and enzymatic production were reviewed. In addition, modelling of the process and energetic and financial analysis is presented, showing that microaerobic pretreatment is commercially attractive under certain conditions. Finally, challenges and future perspectives were also highlighted to promote the development of microaeration as a pretreatment before AD.

A Comparative Review of Established Diets for Prevention of Cardiovascular Disease and Newer Dietary Strategies.

As part of a population-based approach to combating obesity, the American Heart Association has published specific dietary guidelines for the management of obesity and cardiovascular disease prevention. These guidelines give a primary view of healthy dietary changes and goals which may reduce cardiovascular risk. The American Heart Association guideline on Cardiovascular Prevention focuses on the benefits of a Plant-Based Diet and the Mediterranean diet. In addition to these recommendations, several other diets exist with variable long-term cardiovascular outcomes. In recent years, the ketogenic and intermittent fasting diets have been emerging and have garnered their own respective followings as weight loss strategies, and we will include them in our discussion of the potential long-term benefits related to cardiovascular risks. As the guidelines emphasize, all of the diets we will cover throughout this review must be discussed at the level of the individual patient with their primary care provider, and cannot be exercised without informed consent regarding the potential outcomes. Further research is required, and caution is advised before prescribing any of these diets to patients in the long-term, due to the potential to exacerbate cardiovascular risk factors.

Bioremediation potential of Sargassum sp. biomass to tackle pollution in coastal ecosystems: Circular economy approach.

During the past years, the ecological integrity and biodiversity of marine ecosystems have been highly threatened due to the controlled or uncontrolled release of high concentrations of pollutants generated through anthropogenic activities. The occurrence of environmentally related hazardous pollutants, such as toxic elements, and recalcitrant compounds in various environmental matrices has raised increasing concern. Different technologies have been developed for efficient removal and complete mitigation or degradation of these toxic elements from the aquatic environment. Among them, biosorption and bioaccumulation by renewable and biodegradable sources are of supreme interest and have not been reviewed much. For instance, the invasive seaweed Sargassum sp. has been spotted as a cost-effective natural material to capture targeted pollutants from the coastal ecosystem, which is currently becoming a pressing problem, around the globe, due to its unusual proliferation near tropical shores. This review is an effort to cover the left behind gap to present the multifunctional potentialities of Sargassum sp. biomass. Herein, salient information is given to highlight the potential of Sargassum sp. biomass for environmental decontamination with particular focus to coastal ecosystems. Bioremediation mechanisms, challenges of implementation and factors involved in adsorption and absorption of pollutants by seaweeds are also discussed in this review. Against this background, a circular economy perspective is given for the integrated use of the algal raw material. The up-taken pollutants can be recovered and reintegrated into the value chain of industrial processes, while residual biomass is refined to obtain added-value products as bioactive compounds with potential applications for biofuel, agriculture, cosmetics, nutraceutical, pharmaceutical industries among others, to make the most of renewable resources.

Intraoperative radiotherapy (IORT) for surgically resected brain metastases: outcome analysis of an international cooperative study.

BACKGROUND AND OBJECTIVE: The ideal delivery of radiation to the surgical cavity of brain metastases (BMs) remains the subject of debate. Risks of local failure (LF) and radiation necrosis (RN) have prompted a reappraisal of the timing and/or modality of this critical component of BM management. IORT delivered at the time of resection for BMs requiring surgery offers the potential for improved local control (LC) afforded by the elimination of delay in time to initiation of radiation following surgery, decreased uncertainty in target delineation, and the possibility of dose escalation beyond that seen in stereotactic radiosurgery (SRS). This study provides a retrospective analysis with identification of potential predictors of outcomes. METHODS: Retrospective data was collected on patients treated with IORT immediately following surgical resection of BMs at three institutions according to the approval of individual IRBs. All patients were treated with 50kV portable linear accelerator using spherical applicators ranging from 1.5 to 4.0 cm. Statistical analyses were performed using IBM SPSS with endpoints of LC, DBC, incidence of RN, and overall survival (OS) and p < 0.05 considered significant. RESULTS: 54 patients were treated with IORT with a median age of 64 years. The most common primary diagnosis was non-small cell lung cancer (40%) with the most common location in the frontal lobe (38%). Median follow-up was 7.2 months and 1-year LC, DBC, and OS were 88%, 58%, and 73%, respectively. LMD was identified in 2 patients (3%) and RN present in 4 patients (7%). The only predictor of LC was extent of resection with 1-year LC of 94% for GTR versus 62% for STR (p = 0.049). CONCLUSIONS: IORT is a safe and effective means of delivering adjuvant radiation to the BM resection cavities with high rates of LC and low incidence of RN. Further studies are warranted directly comparing LC outcomes to SRS.

Bleeding assessment in female patients with the Hermansky-Pudlak syndrome-A case series.

INTRODUCTION: The Hermansky-Pudlak syndrome (HPS) is an autosomal recessive rare disorder characterized by oculocutaneous albinism, bleeding diathesis, chronic granulomatous colitis and/or pulmonary fibrosis. HPS is the most common single-gene disorder in Puerto Rico with a prevalence of 1:1,800 in the Northwest of the island. Risk of menorrhagia and post-partum hemorrhage (PPH) in cases of women with HPS have been described in the medical literature, but data regarding comprehensive description of bleeding diathesis remains lacking. For this reason, we aim to identify bleeding events using the International Society on Thrombosis and Hemostasis Bleeding Assessment Tool (ISTH-BAT), a standardized quantitative tool that translates the range of severity of bleeding symptoms into a cumulative bleeding score (BS). OBJECTIVE: To use the ISTH-BAT in HPS in order to describe bleeding symptoms and allow for comparison with other inherited bleeding disorders. METHODS: Puerto Rican females and adult participants with HPS based on genetic linkage were enrolled. The ISTH-BAT was administered and results were identified using descriptive statistical analysis. RESULTS: Questionnaire answers of twelve women with HPS-1 and HPS-3 were evaluated. Participants' mean BS was HPS-1 (11.4) and HPS-3 (8.0) Participants with HPS-1 and HPS-3 reported abnormal bleeding events that presented during dental extractions, menorrhagia, surgical interventions, gastrointestinal, oral cavity and post-partum. Patients with history of pulmonary fibrosis (PF) showed a higher mean bleeding score than those who had no history of PF. CONCLUSIONS: Female patients with HPS type 1 and 3 experienced abnormal bleeding events according to the ISTH-BAT bleeding score. Bleeding medications were inconsistently used and varied independently from healthcare professionals. The benefits of this study were to understand the history of bleeding complications in patients with HPS type 1 and 3 using an international validated system. The results of this study will help design strategies to improve the care we provide to this population.

Bioreactors for Cardiac Tissue Engineering.

The advances in biotechnology, biomechanics, and biomaterials can be used to develop organ models that aim to accurately emulate their natural counterparts. Heart disease, one of the leading causes of death in modern society, has attracted particular attention in the field of tissue engineering. To avoid incorrect prognosis of patients suffering from heart disease, or from adverse consequences of classical therapeutic approaches, as well as to address the shortage of heart donors, new solutions are urgently needed. Biotechnological advances in cardiac tissue engineering from a bioreactor perspective, in which recapitulation of functional, biochemical, and physiological characteristics of the cardiac tissue can be used to recreate its natural microenvironment, are reviewed. Detailed examples of functional and preclinical applications of engineered cardiac constructs and the state-of-the-art systems from a bioreactor perspective are provided. Finally, the current trends and future directions of the field for its translation to clinical settings are discussed.

Thermo-separating polymer-based aqueous two-phase systems for the recovery of PEGylated lysozyme species.

Fractionation of native, mono and di-PEGylated lysozyme was performed in 36 different polymer-polymer aqueous two-phase systems using UCON as a phase-forming component. After a discrete partition analysis, dextran 75 kDa-UCON, volume ratio 3, tie-line length 35% w/w; ficoll 70 kDa-UCON, volume ratio 1, tie-line length 45% w/w and a PEG 8 kDa-UCON volume ratio 3, tie-line length 65% w/w systems were selected for optimization via salt addition and to observe the behavior of the lysozyme species in mixtures. The dextran-UCON and the PEG-UCON systems with 75 mM NaCl showed effectiveness in separating 75% and 87% of mono-PEGylated lysozyme from the rest of the lysozyme species in the top and bottom phases, respectively. These results are an advancement in incorporating these extractions in different processes since the use of UCON simplifies the removal of the polymers, providing the opportunity of recycling it to the operation.

A Text Mining Approach to the Text Difficulty of Latin American Peace Agreement / "Una aproximación por minería de texto a la dificultad textual de los acuerdos de paz en Latinoamérica"

Abstract A peace agreement was recently subjected to a plebiscite as a solution to finish the Colombian armed conflict. With 62.57% of abstention, 18.44% of the Colombian electorate rejected this agreement. This paper aims to propose a methodological approach that shows how to linguistically analyze peace agreements as political products that are acceptable or not according to their text difficulty. Given the socio-political similarities of the armed conflicts of Colombia, Guatemala, and El Salvador, we scrutinized with sufficient computational detail these peace agreements. The results revealed that the text difficulty of these accords was more appropriate for a person with at least 19 years of education, suggesting that these sort of texts are not written for broader and less-educated audiences.

Resumen El gobierno de Colombia sometió recientemente a plebiscito un acuerdo de paz para dar por finalizado su conflicto armado. Para este plebiscito la abstención electoral fue del 62.57% y solo el 18.44% de los votantes rechazó la implementación del acuerdo. El objetivo de este trabajo es proponer una aproximación metodológica que muestre cómo analizar los acuerdos de paz como productos políticos que pueden ser aceptables o no según su dificultad textual. Dadas las semejanzas socio-políticas de los conflictos armados en Colombia, Guatemala y El Salvador, en este trabajo se muestra con suficiente detalle computacional el análisis por minería de texto de los acuerdos de paz celebrados en estos países. Los resultados revelaron que la dificultad textual de estos acuerdos exige un nivel educativo de al menos 19 años de educación formal, lo que sugiere que ese tipo de documentos no suelen redactarse para audiencias más numerosas con menores niveles de educación formal.

Electrochemical Biosensors: A Solution to Pollution Detection with Reference to Environmental Contaminants.

The increasing environmental pollution with particular reference to emerging contaminants, toxic heavy elements, and other hazardous agents is a serious concern worldwide. Considering this global issue, there is an urgent need to design and develop strategic measuring techniques with higher efficacy and precision to detect a broader spectrum of numerous contaminants. The development of precise instruments can further help in real-time and in-process monitoring of the generation and release of environmental pollutants from different industrial sectors. Moreover, real-time monitoring can also reduce the excessive consumption of several harsh chemicals and reagents with an added advantage of on-site determination of contaminant composition prior to discharge into the environment. With key scientific advances, electrochemical biosensors have gained considerable attention to solve this problem. Electrochemical biosensors can be an excellent fit as an analytical tool for monitoring programs to implement legislation. Herein, we reviewed the current trends in the use of electrochemical biosensors as novel tools to detect various contaminant types including toxic heavy elements. A particular emphasis was given to screen-printed electrodes, nanowire sensors, and paper-based biosensors and their role in the pollution detection processes. Towards the end, the work is wrapped up with concluding remarks and future perspectives. In summary, electrochemical biosensors and related areas such as bioelectronics, and (bio)-nanotechnology seem to be growing areas that will have a marked influence on the development of new bio-sensing strategies in future studies.

Bio-based materials with novel characteristics for tissue engineering applications - A review.

Recently, a wider spectrum of bio-based materials and materials-based novel constructs and systems has been engineered with high interests. The key objective is to help for an enhanced/better quality of life in a secure way by avoiding/limiting various adverse effects of some in practice traditional therapies. In this context, different methodological approaches including in vitro, in vivo, and ex vivo techniques have been exploited, so far. Among them, bio-based therapeutic constructs are of supreme interests for an enhanced and efficient delivery in the current biomedical sector of the modern world. The development of new types of novel, effective and highly reliable materials-based novel constructs for multipurpose applications is essential and a core demand to tackle many human health related diseases. Bio-based materials possess several complementary functionalities, e.g. unique chemical structure, bioactivity, non-toxicity, biocompatibility, biodegradability, recyclability, etc. that position them well in the modern world's materials sector. In this context, the utilization of biomaterials provides extensive opportunities for experimentation in the field of interdisciplinary and multidisciplinary scientific research. With an aim to address the global dependence on petroleum-based polymers, researchers have been redirecting their interests to the engineering of biological materials for targeted applications in different industries including cosmetics, pharmaceuticals, and other biotechnological or biomedical applications. Herein, we reviewed biotechnological advancements at large and tissue engineering from a biomaterials perspective in particular and envision directions of future developments.

Assessment of microalgae and nitrifiers activity in a consortium in a continuous operation and the effect of oxygen depletion

Background: Industrial wastewaters with a high content of nitrogen are a relevant environmental problem. Currently, treatments to remove nitrogen are not efficient, so is necessary to develop alternative methods. The objective of this study is to investigate a consortium of microalgae - nitrifying, that due to the symbiosis between them could be an interesting alternative. Results: In this study, it was possible to obtain a consortium of nitrifying bacteria (NB) and microalgae (MA) capable of operating with low requirements of dissolved oxygen, using aerobic sludge from wastewater treatment plants. During the operation, this consortium presents removal percentages above 98% of ammonia, even at concentrations of DO of 0.5 mg O2 L-1. It is estimated that the removal was caused both by the action of nitrifying bacteria and microalgae. It was determined that approximately 60% of the ammonia feed was oxidized to nitrate by nitrifying bacteria, while the algae assimilated 40% of the nitrogen feed at steady state. A methodology for measuring the specific activities of nitrifying bacteria and microalgae by comparing the rates in the variation inorganic nitrogen compounds was established with satisfactory results. An average specific activity of 0.05 and 0.02 g NH4 + gVSS-1 d-1 for nitrifying bacteria and microalgae was determined, respectively. Conclusions: The consortium it can be obtained in a single continuous operation, and has a high capacity for nitrogen removal with low oxygen content. The consortium could prove to be a more economical method compared to traditional.

Valores personales en estudiantes y profesionales de enfermería / Personal Values among Students and Nurses / Valores pessoais em estudantes e profissionais de enfermagem

El estudio explora el contraste intergeneracional entre valores personales en estudiantes y profesionales de enfermería y su ajuste a las predicciones de la teoría del cambio de valores. La muestra estuvo formada por 369 estudiantes y profesionales de enfermería distribuidos en tres grupos: estudiantes (n = 150), profesionales menores de 40 años (n = 114), y profesionales entre 41 y 60 años (n = 105). Los participantes informaron de sus valores en orden de prioridad en un cuestionario abierto. Los informes se organizaron en categorías de valores para analizar las diferencias entre grupos. Se encuentran dos tendencias en el informe de valores personales, que se ajustan en algunos casos a lo predicho por la teoría del cambio de valores y, en otros casos, la contradicen. Se discute la importancia de estos hallazgos y la necesidad de potenciar una formación orientada hacia los valores de la profesión de enfermería. El estudio contribuye al conocimiento del cambio de valores personales en profesionales de enfermería.

The study explores the generational contrast between personal values in students and nurses and their adjustment to the predictions of the theory of changing values. The sample was comprised of 369 students and nurses who were divided into three groups: students (n = 150), nurses under age 40 (n = 114), and nurses between 41 and 60 years of age (n = 105). The participants reported their values on an open questionnaire, in order of priority. The reports were organized into value categories to analyze the differences between groups. Two trends were found in the report on personal values: in some cases, they fit what is predicted by the theory of changing values; in others, they contradict it. The importance of these findings and the need to empower training oriented towards the values of the nursing profession is discussed. The study contributes to what we know about the change in personal values among nursing professionals.

Este estudo explora o contraste intergeracional entre valores pessoais em estudantes e profissionais de enfermagem e seu ajuste às predições da teoria da mudança de valores. A amostra esteve conformada por 369 estudantes e profissionais de enfermagem distribuídos em três grupos: estudantes (n = 150), profissionais menores de 40 anos (n = 114) e profissionais entre 41 e 60 anos (n = 105). Os participantes informaram sobre seus valores em ordem de prioridade num questionário aberto. Os relatórios foram organizados por categorias de valores para analisar as diferenças entre grupos. Constataram-se duas tendências no relatório de valores pessoais que se ajustam, em alguns casos, ao dito pela teoria da mudança de valores; em outros casos, contradizem-na. Discute-se a importância dessas constatações e a necessidade de potencializar uma formação orientada aos valores da profissão de enfermagem. O estudo contribui para o conhecimento da mudança de valores pessoais em profissionais de enfermagem.

Occult HBV infection: a faceless enemy in liver cancer development.

The hepatitis B virus (HBV) represents a worldwide public health problem; the virus is present in one third of the global population. However, this rate may in fact be higher due to occult hepatitis B virus infection (OBI). This condition is characterized by the presence of the viral genome in the liver of individuals sero-negative for the virus surface antigen (HBsAg). The causes of the absence of HBsAg in serum are unknown, however, mutations have been identified that produce variants not recognized by current immunoassays. Epigenetic and immunological host mechanisms also appear to be involved in HBsAg suppression. Current evidence suggests that OBI maintains its carcinogenic potential, favoring the progression of fibrosis and cirrhosis of the liver. In common with open HBV infection, OBI can contribute to the establishment of hepatocellular carcinoma. Epidemiological data regarding the global prevalence of OBI vary due to the use of detection methods of different sensitivity and specificity. In Latin America, which is considered an area of low prevalence for HBV, diagnostic screening methods using gene amplification tests for confirmation of OBI are not conducted. This prevents determination of the actual prevalence of OBI, highlighting the need for the implementation of cutting edge technology in epidemiological surveillance systems.

Value Change and Post-modernism: A Preliminary Study of a German Sample

This study explores the reporting of personal values in a sample of German participants through means of a questionnaire using open questions. The sample consisted of a total of 255 participants (60% female and 40% male) with an age range of between 18 and 100 years old. The participants indicated their personal values openly and in order of priority. The results showed that participants primarily considered personal values to be those related to basic life concerns (e.g. family, friendship, health) and to social relations (e.g. honesty, reliability, empathy). A significant trend was found among middle-age participants (31-60 years old) to report values of a political-social nature, whilst older generations (61+ years old) tended to give less importance to values related to social relations. The results were discussed making reference to the socio-cultural change related to post-modernism and were compared and contrasted with the results of major value surveys obtained in sociological studies (AU)

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Congenital ranula.

This article presents 3 cases of infants with congenital ranula, a rare salivary gland pathology. Pathophysiology is discussed, differential diagnosis, and different treatment choices are explained.

Habilidades matemáticas en alumnos de bajo nivel sociocultural / Mathematical skills in students from a low social and cultural stratum / Habilidades matemáticas em alunos de baixo nível sociocultural

La temática abordada es la calidad de la educación básica en México. Con el propósito de dar cuenta del proceso de desarrollo de diversas habilidades matemáticas en alumnos de primer grado de primaria, se realizó un estudio longitudinal en el cual participaron 169 alumnos de estrato sociocultural bajo. Se aplicó un instrumento referido a criterio y basado en análisis de tareas, que permitió analizar los aciertos y errores a lo largo del ciclo escolar 2004-2005. Los resultados indican que los alumnos ingresaron con deficiencias preacadémicas, que se desarrollaron algunas habilidades, pero que los niveles de aptitud matemática fueron bajos al finalizar el curso. Se discuten las implicaciones de los resultados y se proponen alternativas para la educación, basadas en el modelo conductual de desarrollo psicológico.

In this paper, the quality of primary education in Mexico is dealt with A longitudinal study was carried out with the aim of analyzing the process of development for various mathematical skills with first grade students; 169 students from a low social and cultural stratum participated. An instrument referred to criteria and based on task analysis was used to analyze the errors and the correct responses throughout the 2004-2005 school year Results show that students had enrolled with previous academic deficiencies and developed some skills; however, the levels of mathematical skills were still low at the end of the school term. The implications of these results are discussed and some alternatives for education based on a behavioural model of psychological development are proposed.

Neste artigo, é examinada a qualidade do ensino primária em México. Para dar conta do processo de desenvolvimento de várias habilidades matemáticas nos alunos do primeiro grau de ensino primária, foi realizado um estudo longitudinal em que participaram 169 alunos de estrato sociocultural baixo. Foi aplicado um instrumento referido a critério e baseado em análise de tarefas, que possibilitou a análise de acertos e erros durante o ciclo escolar 2004-2005. Os resultados sinalam que os alunos tenham deficiências pré-acadêmicas, que desenvolveram algumas habilidades, mais que os níveis de aptitude matemática foram baixos ao final do curso. São discutidas as implicações dos resultados e são propostas alternativas para a educação, baseadas no modelo condutista de desenvolvimento psicológico.

Habilidades lingüísticas en niños de estrato sociocultural bajo, al iniciar la primaria / Linguistic skills of children from a low socioeconomic level at the beginning of elementary education

El objetivo de este estudio fue evaluar las habilidades lingüísticas con que ingresan a la educación básica primaria alumnos de estrato socioeconómico bajo. Participaron 262 alumnos de primer grado, inscritos en ocho grupos de escuelas públicas del Estado de México, con una media de 5.7 años de edad. Se aplicó individualmente a cada alumno el Instrumento para Evaluar Habilidades Precurrentes para la Lectura (EPLE), de Vega (1991), al inicio del ciclo escolar 2004-2005. El análisis estadístico con el programa SPSS arrojó como resultado un puntaje promedio de 77 (DS=14), que corresponde al 57% de la calificación total del EPLE, lo que puede indicar que los alumnos ingresaron al primer grado de primaria con un nivel insatisfactorio de conductas lingüísticas. Se observan ligeras diferencias en las ejecuciones de los alumnos, a favor de quienes ingresaron con seis años de edad y con mayor número de años cursados en preescolar. Se discuten las implicaciones del bajo nivel lingüístico mostrado por los participantes y se proponen alternativas basadas en la investigación psicológica conductual.

The purpose of this study was to assess the linguistic skills of children from a low socioeconomic background who were beginning their elementary education. A total of 262 first-grade students with an average age of 5.7 years old and who were enrolled in 8 groups of public schools from Mexico State, participated in this project. The instrument used was the Precurrent Skills for Reading Assessment (PSRA) by Vega (1991), and was administered individually to each student at the beginning of the 2004-2005 school year. The statistical analysis using SPSS showed a mean score of 77 (SD=14), which corresponds to 57% of the total PSRA score. These results may indicate that first grade students have an unsatisfactory level of linguistic behaviour. Slight differences in the students’ performances were observed. A slightly better performance was shown by those first grade students who were 6 years old at the beginning of the school year and who had previously received pre-school education. The implications of the poor linguistic level shown by the participants are discussed, and some alternatives based on the behavioural research, are proposed.