Knowledge, Attitude, and Practices toward the Novel Coronavirus Infection in Dental Laboratories in the Kingdom of Saudi Arabia: Cross-Sectional Study.

This study aimed to assess dental technologists/technicians knowledge, attitudes, and practice during the COVID-19 pandemic in Saudi Arabia. This cross-sectional study was conducted among workers in dental laboratories in Saudi Arabia. A validated questionnaire was developed based on previous literature and distributed online. The questionnaire assessed the study population's knowledge, attitude, and practice regarding infection control protocol for COVID-19. Descriptive statistics of categorical variables were conducted. The response rate was 58.3%, where most respondents are male at a rate of 97%. Most of the participants (82%) were aware of the various infection control measures for COVID-19 in a dental laboratory. Forty-eight participants showed a level of knowledge range between 80 and 100%, while 27 and 30 participants reported moderate and low level of knowledge, respectively. Only 67% of the participants strongly agree that dental cast and fabricated/repaired restorations should be transferred in a very close and protective package to control infection. About 70% of participants indicated they always wear protective gear/goggles and should be warned while working in the dental laboratory. As the pumice slurry was utilized frequently to clean and polish the prostheses, 66% of participants agreed that pumice slurry should be always changed regularly. Regarding COVID-19 infection control procedures, dental technologists/technicians showed good knowledge, attitude, and practice; however, considering how infectious the virus is, more measures should be taken into consideration. Effective communication and collaboration between the laboratory and the dental clinic are essential for infection control.

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth.

In the realm of regenerative medicine and therapeutic applications, stem cell research is rapidly gaining traction. Dental pulp stem cells (DPSCs), which are present in both deciduous and permanent teeth, have emerged as a vital stem cell source due to their accessibility, adaptability, and innate differentiation capabilities. DPSCs offer a readily available and abundant reservoir of mesenchymal stem cells, showcasing impressive versatility and potential, particularly for regenerative purposes. Despite their promise, the main hurdle lies in effectively isolating and characterizing DPSCs, given their representation as a minute fraction within dental pulp cells. Equally crucial is the proper preservation of this invaluable cellular resource. The two predominant methods for DPSC isolation are enzymatic digestion (ED) and outgrowth from tissue explants (OG), often referred to as spontaneous growth. This protocol concentrates primarily on the enzymatic digestion approach for DPSC isolation, intricately detailing the steps encompassing extraction, in-lab processing, and cell preservation. Beyond extraction and preservation, the protocol delves into the differentiation prowess of DPSCs. Specifically, it outlines the procedures employed to induce these stem cells to differentiate into adipocytes, osteoblasts, and chondrocytes, showcasing their multipotent attributes. Subsequent utilization of colorimetric staining techniques facilitates accurate visualization and confirmation of successful differentiation, thereby validating the caliber and functionality of the isolated DPSCs. This comprehensive protocol functions as a blueprint encompassing the entire spectrum of dental pulp stem cell extraction, cultivation, preservation, and characterization. It underscores the substantial potential harbored by DPSCs, propelling forward stem cell exploration and holding promise for future regenerative and therapeutic breakthroughs.

Psychological impact of COVID-19 lockdown period on students of healthcare colleges.

Background: The coronavirus disease 2019 (COVID-19) pandemic globally caused depression, anxiety, and post-traumatic stress. Mental health was affected by social separation, curfews, school and business closures, economic deterioration, and government objectives. This study intends to assess the influence of the post-COVID-19 lockdown period on the psychological health of students enrolled in health colleges at King Saud University, Saudi Arabia. Materials and Methods: A cross-sectional study was conducted among students of healthcare colleges at King Saud University. We used a validated questionnaire of the Generalized Anxiety Disorder Assessment (GAD-7) and the Perceived Stress Scale (PSS-10). The questionnaire was sent to participants via google forms link through WhatsApp, e-mail, and other social media platforms. A total of 311 participants responded to the questionnaire. Descriptive analysis and a Chi-square test were performed for qualitative data analysis. Results: Among study participants, males were 50.4% and females were 49.6%. About 63% of the students attended a training session on COVID-19 prevention strategies. About 37% of the participants were from the College of Medicine, 27% were from the College of Dentistry, 21% from the College of Pharmacy, 5% from the College of Applied Medical Sciences, and 10% from the College of Nursing. The average anxiety score was 9.84, and the perceived stress score (PSS) was 23.95. 44.1% of students reported mild anxiety, and 1.6% suffered severe anxiety. High stress was perceived by 24.1% of the students, and 75.2% perceived a moderate stress level. No significant association was reported between gender, stress, and anxiety. Similarly, no association was found between stress and the year of study. However, a significant association was found between the year of study and anxiety (P = 0.015). Conclusion: To the limitation of the study, the analysis revealed that COVID significantly influences students' psychological health. Such findings can be used to develop psychological interventions and susceptible populations and execute appropriate mental health policies and programs targeting student communities.

In vitro comparison of antifungal activity of conventional alcohol sprays and antimicrobial photodynamic therapy on acrylic denture resin.

BACKGROUND: Traditionally, alcohol sprays are used for disinfection of acrylic-base denture surfaces. A limited number of studies have assessed the role of antimicrobial photodynamic therapy (aPDT) in this regard; however, it remains debatable whether conventional alcohol sprays are superior to aPDT in terms of antifungal activity or vis versa. OBJECTIVE: The aim of the present in vitro study is to compare the antifungal activity of conventional alcohol sprays and aPDT on acrylic denture resin. METHODS: Individuals wearing complete dentures at least on one arch were included. Dentures were randomly divided into three groups. Groups 1-3 were disinfected with an alcohol-based antiseptic spray and aPDT, respectively. Assessment of oral yeast growth was done using swab samples. The culture mediums were incubated at 37∘C for 72 hours and viewed through a microscope. The numbers of colony forming units (CFU/ml) were determined. P< 0.05 were considered statistically significant. RESULTS: At baseline, the mean CFU/ml in Groups 1-3 were comparable. After disinfection, a statistically significant reduction in microbial CFU/ml was observed in Groups 1 (P< 0.05) and 2 (P< 0.05) compared with baseline. In Group 3, there was no difference in CFU/ml throughout the study. After disinfection, there was no difference in microbial CFU/ml in dentures in Groups 1 and 2. CONCLUSION: Conventional alcohol sprays are as effective as aPDT towards reducing oral yeasts CFU/ml on acrylic denture resin.

Knowledge of the monkeypox 2022 outbreak among dental hygienists and students in Saudi Arabia: A cross-sectional study.

OBJECTIVE: This study aimed to assess the knowledge of monkeypox infection among dental hygiene professionals and students in Saudi Arabia. METHODS: A cross-sectional study was conducted among a convenience sample of dental hygiene professionals (n = 259). The questionnaire was developed based on previous literature, and descriptive analysis and a Chi-square test were performed. RESULTS: A total of 159 dental hygienists responded to the questionnaire. Results indicated that only 1.7% had good knowledge, 7.7% had moderate knowledge and 90.6% had low knowledge of the monkeypox outbreak. The mean knowledge scores varied among dental hygiene students, practitioners and faculty members. Significant group differences were observed for some questions (p < 0.05). CONCLUSION: Our study concludes that dental hygienists had moderate to low level of knowledge of the monkeypox infection and its implications for oral health and patient care, indicating a need for more education on the subject.

Andersen health care utilization model: A survey on factors affecting the utilization of dental health services among school children.

BACKGROUND: Children's quality of life, academic performance, and future achievement can all be negatively affected by poor dental health. The present study aimed to assess the need for dental health services and the factors influencing their utilization using the Andersen health care utilization model among school children. METHODS: The current cross-sectional study was conducted among schoolchildren aged 13 to 15 in Bangalore, India (n = 1100). A questionnaire was developed using the concepts of the Andersen healthcare usage model. The parents of the children filled out the questionnaire. The factors were investigated using bivariate analysis and multivariate logistic regression analysis. RESULTS: About 78.1% of the children did not utilize dental health services. Regarding the reasons for not visiting a dentist, 65.8% said they did not have a dental problem, and 22.2% said they could not afford it. Bivariate analysis showed that age, gender, education level, occupation of the family's head of household, monthly family income, socioeconomic status, perceived oral health problems, accessibility of dental health facilities, and parental attitudes toward their children's oral health were significantly associated with using dental health services (p<0.05). Multiple regression analysis showed dental health service utilization was directly related to age (OR = 2.206), education, family size (OR = 1.33), and brushing frequency twice a day (OR = 1.575) with no significant relationship between distance to reach the dental facility, the number of dental visits, and socioeconomic status. CONCLUSION: Dental health service utilization was low in the past year. The age, number of family members, parent's education level, travel time to the dental facility, the child's oral health behaviors, and positive parental attitude all play a role in a children's utilization of dental health service.

Perception of COVID-19 Booster Dose Vaccine among Healthcare Workers in India and Saudi Arabia.

Background: COVID-19 vaccines were made available to the public by the end of 2020. However, little is known about COVID-19 booster dose (CBD) vaccine perception among healthcare workers (HCW) worldwide. The present study aims to assess the perception of CBD vaccines among healthcare workers in India and Saudi Arabia (SA). Methods: A cross-sectional study was conducted among HCWs in two countries, India and SA. Data were gathered through the use of a self-administered questionnaire. A convenience sampling technique was utilized to collect the data. Results: A total of 833 HCW responses were collected from the two countries, with 530 participants from India and 303 participants from SA responding to the questionnaire. Among them, 16% from India and 33% from SA were unwilling to take a CBD (p < 0.005). The primary reasons for not being willing were concerns about whether the vaccine would be effective (32%) and concerns about probable long-term side effects (31%). Concerns about not knowing enough about the vaccination (30%) and the possibility of long-term side effects (28%) were the primary concerns in SA. Regression analysis showed that males, urban residents, and post-graduates were more willing to take the CBD. Conclusion: There is a good perception of CBD and some hesitancy in receiving the booster dose among HCWs in both countries. The introduction of personalized education, risk communication, and deliberate policy could help to reduce the number of people who are unwilling to take a booster shot.

Efficacy of antimicrobial photodynamic therapy versus antiviral therapy in the treatment of herpetic gingivostomatitis among children: Aa randomized controlled clinical trial.

AIM: The aim of the present study was to evaluate the effect of antimicrobial photodynamic therapy (aPDT) as an adjunctive treatment to topical antiviral therapy for the treatment of children having herpetic gingivostomatitis. MATERIALS AND METHODS: 45 individuals (age group 12-18 years) with herpetic gingivostomatitis (HG) were divided into three groups on the basis of provision of treatment. (a) Group A: topical anti-viral therapy (TAT) (n = 14, mean age = 17.0 years) (b) Group B: antimicrobial photodynamic therapy (aPDT) (n = 15, mean age =17.7 years) and (c) Group C: topical anti-viral therapy + adjunctive aPDT (n = 16, mean age = 18.0 years) respectively. Pain scores [visual analogue scale (VAS) and McGill Pain Questionnaire (MPQ)] were assessed and HSV-1 was quantified. ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA) was used to compute the pro-inflammatory cytokine including interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-α). The analysis of the mean values and inter group comparisons were evaluated with the Mann-Whitney test. The Friedman test was used to establish the comparison of the changes observed in HSV quantification, pain scores, and pro-inflammatory cytokines. ANOVA tests were employed for the quantification of differences observed at follow-ups. The assessments for the clinical trial were done at baseline, immediate after post-op, two, and four weeks, and three and six months respectively. RESULTS: According to the analysis of the data obtained after the clinical assessment, the three groups reported a decrease in the pain scores, HSV-1 quantification and levels of the pro-inflammatory cytokines. However, Group C (TAT + aPDT) reported improvement in the observed parameters which was statistically significant in comparison to Group A (TAT) and Group B (aPDT) respectively. CONCLUSION: Antimicrobial photodynamic therapy (aPDT) in conjunction with topical antiviral therapy (TAT) helped in reducing the pain scores and pro-inflammatory cytokine levels in herpetic gingivostomatitis among children.

Role of antimicrobial photodynamic therapy in reducing subgingival oral yeasts colonization in patients with peri-implant mucositis.

OBJECTIVE: The aim was to assess the role of antimicrobial photodynamic therapy (aPDT) in reducing subgingival oral yeasts colonization (OYC) in patients with peri-implant mucositis (PIM). METHODS: Patients diagnosed with PIM were included. Patient demographics were recorded and implant placement and prosthetic rehabilitation protocols were retrieved from patients' records. Peri-implant clinical parameters (modified plaque index [mPI], modified bleeding index [mBI] probing depth [PD]) and subgingival OYC and were assessed using standard techniques. All patients were randomly divided into test- and control-groups. In the test-group, patients underwent mechanical debridement (MD) of implant surfaces and supra and sub-gingival peri-implant sulci peri-implant immediately followed by a single session of aPDT. In the control-group, patients underwent MD alone. Peri-implant clinical parameters and OYC were re-assessed at 3-months' follow-up. Sample-size estimation was done on data from a pilot investigation and group-comparisons were done using the paired t- and Mann Whitney U-tests. Correlation between age, mPI, mBI, PD and OYC at baseline and 3-months' follow-up was assessed using regression analysis models. A statistically significant difference between the groups was recorded when P-values were less than 0.01. RESULTS: Thirty-four individuals (17 and 17 in the test- and control groups, respectively) were included. There was no significant difference in the mean age, scores of mPI, mBI, PD and OYC among patients in the test- and control-groups at baseline. At 3-months of follow-up, there was a statistically significant reduction in scores of mPI (P<0.001), mBI (P<0.001), PD (P<0.001) and OYC (P<0.001) among patients in the test- compared with the control-groups. There was no significant correlation between age, mPI, mBI, PD and OYC in both groups. CONCLUSION: In the short term, a single session of aPDT as an adjunct to MD is effective in reducing peri-implant soft tissue inflammation and OYC in patients with PIM.

Antimicrobial efficacy and topographical alterations of photodynamic therapy versus conventional antimicrobials on contaminated zirconia ceramic in vitro.

PURPOSE: The aim of this in vitro study was to assess the efficacy of antimicrobial photodynamic therapy (aPDT), neodymium-doped yttrium aluminium garnet (Nd:YAG) laser, and two other disinfecting agents against P. gingivalis and T. forsythia that colonized over zirconia ceramics and to evaluate if the disinfecting protocols result in zirconia surface alteration. MATERIALS AND METHODS: The experiment was conducted on pre-sintered, commercially available Yttria Stabilized Zirconia (Y-TZP) ceramic blocks. The bacterial strains of P. gingivalis and T. forsythia were used to contaminate the zirconia specimens. The infected zirconia specimens were randomly divided into 4 groups (n=20/group): aPDT, Nd:YAG laser, hydrogen peroxide (H2O2), and chlorhexidine groups. The viability of bacteria was assayed using the MTT protocol. The surface roughness (Ra) of all zirconia specimens was estimated using a profilometer and a drop-shape analyzer was used to evaluate the contact angle using a special sessile drop method. RESULTS: A statistically significant reduction by the aPDT group is noticed for P. gingivalis and T. forsythia species compared to other disinfection methods (p<0.05). The second highest reduction was seen for chlorhexidine followed by Nd:YAG laser. The least reduction was demonstrated for the hydrogen peroxide group. Statistically, there was no significant difference in Ra scores between the four groups. However, the contact angles were significantly reduced from the zirconia specimens after the aPDT method which indicates a hydrophilicity increase compared to other groups. The SFE scores of all decontamination protocols from highest to lowest were aPDT (41.68), chlorhexidine (39.83), Nd:YAG (34.52), and H2O2 (29.88). CONCLUSIONS: Antimicrobial photodynamic therapy demonstrated a high antibacterial efficacy over zirconia ceramic surface without altering surface topography.

Effectiveness of photodynamic therapy on bonding strength and failure modes of fiber-posts in c-shaped treated root canals.

AIM: The present study aimed to assess the push-out bond strength (PBS) and failure modes of fiber-posts after photodynamic therapy (PDT) and Erbium yttrium scandium gallium garnet (Er,Cr:YSGG) in c-shaped canals. METHODS: Sixty molars with c-shaped root configuration were studied. The endodontic prepared teeth specimen were equally randomized into four groups: i) photodynamic therapy (PDT); ii) Er,Cr:YSGG; iii) chlorhexidine and; iv) control group. Post root filling, 10 mm space was prepared for insertion of post in the root canals. The push-out bond strength was evaluated using Universal testing machine. Modes of failure were evaluated as adhesive, cohesive and admixed. Mean values were computed between groups using Kruskal-Wallis test followed by Bonferroni post hoc test. Comparison between two thirds of root sections were analyzed using Friedman test. P-value was set at 0.05 or less. RESULTS: Specimens treated with PDT reported the highest mean PBS (8.56 ± 2.12 MPa), while the lowest PBS was observed in NaOCl group (6.92 ± 2.21 MPa). Thirteen failures were observed at the adhesive post interface, whereas 22 failures were found at the interface forming between the dentin surface and adhesive. Similarly, 9 failures were categorized as mixed. For PDT group, a sum of 3 failures and 4 failures were noted at the apical and cervical points. CONCLUSION: The specimens treated with PDT showed the highest push out bond strength with least number of failures.in c-shaped root canals.

Nanoparticle incorporated dentin bonding agent to caries effected dentin treated by photodynamic therapy, laser or chlorhexidine.

PURPOSE: This study aims to evaluate the microtensile bond strength (µTBS) and failure modes of graphene nanoplatelets doped with hydroxyapatite (GNP-HA) modified self-etch dentin bonding agent (DBA) to caries effected dentin (CAD) after various conventional and laser disinfection protocols. MATERIALS AND METHODS: Graphene nanoplatelets and HA nanoparticles were doped and incorporated in DBA. Fifty carious human molar teeth were used for the experiment. CAD specimens were divided into five groups (n=10/group) into CAD undergoing no disinfection treatment (Group-CAD), 2% chlorhexidine disinfection (Group-CHX), 6% NaOCl disinfection (Group-NaOCl), methylene blue assisted PDT (Group-MB-PDT), and Er,Cr:YSGG laser application (Group-Er,Cr:YSGG). Following the respective disinfection techniques, the GNP-HA modified adhesive was applied to all treated CAD surface. Subsequently µTBS and failure modes were analyzed. RESULTS: Representative scanning electron microscope images (SEM images) of the synthesized GNP-HA indicated flakes of graphene with varying size lengthwise (>500 nm) with 1-2 nm width. The doped HA showed round and spherical morphology conjugated over the surface of the GNP measuring 40 -100 nm in diameter. CAD group that underwent no treatment represented the highest µTBS (29.43 MPa). Within the tested specimens, MB-PDT (22.37 MPa) and Er,Cr:YSGG (21.85 MPa) showed the highest µTBS values than CHX (15.69 MPa) and NaOCl (11.46 MPa). However, there was no significant difference between MB-PDT and Er,Cr:YSGG (p>0.05) and a significant difference existed between CHX and NaOCl (p<0.05). Equal distribution of cohesive and adhesive failures was noted for CAD, CHX and NaOCl treated specimens. A majority of adhesive failures existed in the MB-PDT groups. While admixed and adhesive failures were higher in Er,Cr:YSGG treated samples. CONCLUSIONS: The outcome of this study suggests that CAD restored by GNP-HA modified self-etch adhesive showed highest bond strength after MB-PDT and Er,Cr:YSGG protocols with low amount of adhesive failure.

Antimicrobial resistance, mechanisms and its clinical significance.

Antimicrobial agents play a key role in controlling and curing infectious disease. Soon after the discovery of the first antibiotic, the challenge of antibiotic resistance commenced. Antimicrobial agents use different mechanisms against bacteria to prevent their pathogenesis and they can be classified as bactericidal or bacteriostatic. Antibiotics are one of the antimicrobial agents which has several classes, each with different targets. Consequently, bacteria are endlessly using methods to overcome the effectivity of the antibiotics by using distinct types of mechanisms. Comprehending the mechanisms of resistance is vital for better understanding and to continue use of current antibiotics. Which also helps to formulate synthetic antimicrobials to overcome the current mechanism of resistance. Also, encourage in prudent use and misuse of antimicrobial agents. Thus, decline in treatment costs and in the rate of morbidity and mortality. This review will be concentrating on the mechanism of actions of several antibiotics and how bacteria develop resistance to them, as well as the method of acquiring the resistance in several bacteria and how can a strain be resistant to several types of antibiotics. This review also analyzes the prevalence, major clinical implications, clinical causes of antibiotic resistance. Further, it evaluates the global burden of antimicrobial resistance, identifies various challenges and strategies in addressing the issue. Finally, put forward certain recommendations to prevent the spread and reduce the rate of resistance growth.

Bactericidal and clinical efficacy of photochemotherapy in acute necrotizing ulcerative gingivitis.

AIM: This study was designed to investigate the clinical and microbiological parameters with photochemotherapy (PCT) against conventional debridement (CD) among patients with necrotizing ulcerative gingivitis (NUG). MATERIALS AND METHODS: Patients with a diagnosis of NUG were divided into two groups: 'Group-PCT' that underwent indocyanine green-mediated PCT with adjunctive mechanical debridement, and Group-CD that underwent mechanical debridement with adjunctive 3 % hydrogen peroxide/chlorhexidine rinse. Clinical inflammatory gingival parameters including full-mouth plaque scores (FMPS), bleeding scores (FMBS), and probing depth (PD) were measured. Counts of Treponema microdentium, Fusobacterium nucleatum and Prevotella intermedia were assessed using polymerase chain reaction technique. All assessments were done at baseline, 3 weeks and 6 weeks. RESULTS: Twenty-six participants were contacted and agreed to participate in the trial. The mean age of participants in group-PCT was 24.5 years while the mean age of patients in group-CD was 26.7 years. FMPS showed significant but equal reduction in both the groups at 3 weeks and 6 weeks (p < 0.01). The reduction in mean FMBS was higher in the group-PCT at 6 weeks follow-up (p < 0.05). All bacterial levels reduced from baseline to follow-up with both PCT and CD groups (p < 0.05). Group-PCT showed significantly reduced counts of T. microdentium and F. nucleatum at 3 weeks and 6 weeks compared with group-CD (p = 0.024). Equal reduction was shown for P. intermedia between both the groups at 3 and 6 weeks, respectively. CONCLUSION: Application of adjunctive photochemotherapy was both clinically and microbiologically effective in the treatment of NUG.

Effect of Non-Surgical Periodontal Therapy on the Serum Sialic Acid Levels in Diabetic Patients with Periodontitis.

Sialic acid (SA), a family of acetylated derivatives of neuraminic acid, an acute phase reactant by itself. It usually occurs as a terminal component at the non-reducing end of carbohydrate chains of glycoproteins and glycolipids. SA participates in multiple physiological functions, such as cell-to-cell interactions, cell migration and proliferation. Diabetes mellitus (DM) is a chronic metabolic disorder characterized by rise in blood glucose level. Periodontitis is a chronic inflammatory disease of the periodontal tissue, leading to destruction of bone surrounding the tooth and ultimately tooth loss. There is a two way relationship between diabetes mellitus and periodontitis. Periodontitis is the sixth complication of diabetes along with retinopathy, nephropathy, neuropathy, macrovascular disease, and altered wound healing. Inflammatory mediators like interleukin-6 and tumor necrosis factor-alpha produced during periodontal inflammation can interfere with the actions of insulin receptors and worsen the glycemic control of diabetic patients. Periodontitis is a major cause of tooth loss, affecting over 300 million people and bacteria associated with periodontitis are also linked with systemic problems like endocarditis, atherosclerosis. Recent work has highlighted a major role for the host sugar sialic acid in the biofilm physiology and host-pathogen interactions of T. forsithya, a key periodontal pathogen. There exists a need for a biomarker, for early detection of disease evolution and more robust therapy efficacy measurements. Serum sialic acids were estimated in Indian population by diphenylamine method and Thiobarbituric acid method. The average values were 68 ± 2.6 mg percent by DPA method and 56 ± 5 mg percent by TBA (thiobarbituric acid assay) method. Age and sex showed no influence on serum sialic acid level. Objectives of the present study was to compare (TSSA) level in healthy subjects, subjects with (CMP) with and without (NIDDM) and its effect on non-surgical periodontal therapy. In the present study, the participants were divided into three groups: Group A, B and C. Group A consists of systemically healthy subjects, Group B consists of subjects with (CMP) while Group C consists of subjects with (CMP) with (NIDDM) and results of this study indicated that, at baseline, there were significant differences between Group A, B and Group C with respect to all the clinical parameters, including (GI), (OHI-S), (PPD), (CAL), (TSSA) and (HbA1c) levels. Thus (TSSA) level could be considered as novel biomarker in the progression of periodontal disease and diabetic status. Periodontitis could be considered as a potential, modifiable, and independent risk factor for the development of diabetes. Early detection of elevated (TSSA) level may help in interpreting the progression of periodontitis, risk of development of diabetes mellitus in future and also to prevent complications.

Analyzing Relationship between Patient and Doctor in Public Dental Health using Particle Memetic Multivariable Logistic Regression Analysis Approach (MLRA2).

In the developing technology managing patient and doctor relationship and communication process is one of the critical factors because the patient may take high risk surgery, treatment and diagnosis. Due to the importance of the patient-doctor relationship, in this paper investigate the various aspects of relation between the doctor and dental problem related patients. During the analyzing process, data has been collected from adults who are facing dental problems and other dental injuries in which data has been gathered from 423 individuals by conducting interviews. The gathered data is investigated with the help of particle-memetic multivariable logistic regression analysis method which examines the patient income, dental surgical details, injuries and other factors relationship has been investigated. From the analyzed data, how the patients are treated by doctors examined for improving the relationship between patient and doctor in public dental health aspects.

Pathogenic features of Streptococcus mutans isolated from dental prosthesis patients and diagnosed cancer patients with dental prosthesis.

Though S. mutans, virulence, and pathogenesis are characterized, reports are limited to the status of its carriage and virulence in patients with oral cancer and prosthesis. In this present study, we investigated the pathogenic characteristics of twenty strains of S. mutans of healthy subjects, fifteen of prosthesis patients, and eleven from oral cancer. The putative virulence gene and other factors, such as the ability to adhere to the oral epithelial cells, production of glycan and lactic acid of these strains were examined. Few of representative isolates of each group were used to see their activity on oral cancer cell line using cell cytotoxicity assay. The isolation rate of S. mutans was significantly more in carcinoma than prosthesis patients and control health group. The production of glycan and latic acid was together high in those isolates derived from prosthesis patients and patients with cancer. Adherence ability of strains isolated from prosthesis patients and cancer patients with oral prosthesis were significantly higher, compared to one isolated from a healthy individual. From our study results, we conclude that prosthesis patients and cancer patients with prosthesis carried a high number of S. mutans in their oral cavities. SIGNIFICANCE AND IMPACT OF STUDY: This study report that prosthesis patients and cancer patients with prosthesis carried a high number of S. mutans in their oral cavities. However, the S mutans are commensals still they have the capability to raise the severity of disease condition due to their ability to produce glycan and lactic acid. In our study, we proved that the adherence to buccal epithelial cells was significantly increased in S. mutans isolates of prosthesis patients and cancer patients. These indicate that in prosthesis patients as well as in cancerous patient's microbes had more potential to cause infection and increase the severity.

Occurrence of vancomycin-resistant Staphylococcus aureus in the oral cavity of patients with dental caries.

Oral streptococci are the major group of microbes isolated from oral microflora. They represent frequent pathogens of infective endocarditis (IE), and it is assumed that in most of the cases oral streptococci are acquired via mucosa layer of oral cavity. Staphylococcus aureus is also frequently isolated from IE as it accounts for 20%-30% of all cases. Vancomycin has been the most reliable therapeutic agent against infections caused by methicillin-resistant S. aureus (MRSA). The main objective of this study was to examine the occurrence of S. aureus species in dental caries specimens. Antimicrobial susceptibility testing of S. aureus to four antibiotics namely vancomycin, linezolid, teicoplanin, and daptomycin was performed. Detection of vancomycin resistance was conducted using polymerase chain reaction. Among the tested 150 strains, 98 were MRSA and of that 54 were vancomycin sensitive and 27 were resistant. All 98 MRSA strains were positive for mecA and 36 yielded pvl, whereas 13 carried vanA and only 2 were positive for vanB. Majority of the isolates showed sensitivity toward daptomycin and linezolid. Strains of S. aureus exhibiting decreased susceptibility to different antibiotics like vancomycin, daptomycin, and linezolid severely compromise the therapeutic alternatives and require a considerable amount of time, public awareness, and integrative health-care strategies to prevent the emergence of resistance to these compounds.

Pain and distress induced by elastomeric and spring separators in patients undergoing orthodontic treatment.

AIMS AND OBJECTIVES: The objective of the present investigation is to evaluate patients' pain perception and discomfort, the duration of pain and the level of self-medication over time during tooth separation, and the effectiveness of elastomeric and spring types of orthodontic separators in Saudi population. MATERIALS AND METHODS: The study group consisted of 30 female adolescent patients who had elastomeric/spring separators as part of their orthodontic treatment. A self-administrated questionnaire comprising 16 multiple choice questions and another with visual analog scale were used to record the patient's pain perceptions at 4 hours, 24 hours, 3 days, 5 days, and 7 days from the time of insertion. The level of pain and discomfort during these time periods were assessed by a visual analog scale. After a separation period of 7 days, the amount of separation was measured with a leaf gauge. Type and frequency of analgesic consumption was also recorded. The Statistical Package for the Social Sciences (SPSS) version 20 (IBM SPSS -Chicago, IL: SPSS Inc.,) was used for statistical analysis. RESULTS: The data showed significant increase in the level of pain at 4 hours, 24 hours, and 3 days from separator placement. The elastomeric separators produced significantly more separation than the spring separators and also caused maximum pain during the first 3 days after insertion. However, there was no significant difference between the score of pain between two separators at all time intervals. CONCLUSION: Both elastomeric and spring separators showed comparative levels of pain and discomfort during the early phase of separation. Elastomeric separators were found to be more effective in tooth separation than spring separators. However, further studies are necessary to substantiate this preliminary observation.

Sex determination using discriminant function analysis in Indigenous (Kurubas) children and adolescents of Coorg, Karnataka, India: A lateral cephalometric study.

Aim: To test the validity of sex discrimination using lateral cephalometric radiograph and discriminant function analysis in Indigenous (Kuruba) children and adolescents of Coorg, Karnataka, India. Methods and materials: Six hundred and sixteen lateral cephalograms of 380 male and 236 females of age ranging from 6.5 to 18 years of Indigenous population of Coorg, Karnataka, India called Kurubas having a normal occlusion were included in the study. Lateral cephalograms were obtained in a standard position with teeth in centric occlusion and lips relaxed. Each radiograph was traced and cephalometric landmarks were measured using digital calliper. Calculations of 24 cephalometric measurements were performed. Results: Males exhibited significantly greater mean angular and linear cephalometric measurements as compared to females (p < 0.05) (Table 5). Also, significant differences (p < 0.05) were observed in all the variables according to age (Table 6). Out of 24 variables, only ULTc predicts the gender. The reliability of the derived discriminant function was assessed among study subjects; 100% of males and females were recognized correctly. Conclusion: The final outcome of this study validates the existence of sexual dimorphism in the skeleton as early as 6.5 years of age. There is a need for further research to determine other landmarks that can help in sex determination and norms for Indigenous (Kuruba) population and also other Indigenous population of Coorg, Karnataka, India.