1.
Prog Clin Biol Res
; 397: 315-25, 1998.
Artículo
en Inglés
| MEDLINE
| ID: mdl-9575572
Asunto(s)
Proteínas de Fase Aguda , Lípido A/metabolismo , Lipopolisacáridos/metabolismo , Glicoproteínas de Membrana , Péptidos/metabolismo , Secuencia de Aminoácidos , Sitios de Unión , Proteínas Portadoras/metabolismo , Técnicas In Vitro , Prueba de Limulus , Datos de Secuencia Molecular , Peso Molecular
2.
Science
; 259(5093): 361-5, 1993 Jan 15.
Artículo
en Inglés
| MEDLINE
| ID: mdl-8420003
RESUMEN
Endotoxin [lipopolysaccharide (LPS)], the major antigen of the outer membrane of Gram-negative bacteria, consists of a variable-size carbohydrate chain that is covalently linked to N,O-acylated beta-1,6-D-glucosamine disaccharide 1,4'-bisphosphate (lipid A). The toxic activity of LPS resides in the lipid A structure. The structural features of synthetic peptides that bind to lipid A with high affinity, detoxify LPS in vitro, and prevent LPS-induced cytokine release and lethality in vivo were defined. The binding thermodynamics were comparable to that of an antigen-antibody reaction. Such synthetic peptides may provide a strategy for prophylaxis and treatment of LPS-mediated diseases.