Asunto(s)
Proteínas/química , Proteínas/metabolismo , Sustitución de Aminoácidos/genética , Bacteriófago T4/enzimología , Bacteriófago T4/genética , Entropía , Cinética , Ligandos , Espectroscopía de Resonancia Magnética , Modelos Moleculares , Movimiento (Física) , Muramidasa/química , Muramidasa/genética , Muramidasa/metabolismo , Conformación Proteica , Pliegue de Proteína , Proteínas/genéticaRESUMEN
The global fold of maltose-binding protein in complex with the substrate beta-cyclodextrin was determined by solution NMR methods. The two-domain protein is comprised of a single polypeptide chain of 370 residues, with a molecular mass of 42 kDa. Distance information in the form of H(N)-H(N), H(N)-CH(3) and CH(3)-CH(3) NOEs was recorded on (15)N, (2)H and (15)N, (13)C, (2)H-labeled proteins with methyl protonation in Val, Leu, and Ile (C(delta1) only) residues. Distances to methyl protons, critical for the structure determination, comprised 77 % of the long-range restraints. Initial structures were calculated on the basis of 1943 NOEs, 48 hydrogen bond and 555 dihedral angle restraints. A global pair-wise backbone rmsd of 5.5 A was obtained for these initial structures with rmsd values for the N and C domains of 2.4 and 3.8 A, respectively. Direct refinement against one-bond (1)H(N)-(15)N, (13)C(alpha)-(13)CO, (15)N-(13)CO, two-bond (1)H(N)-(13)CO and three-bond (1)H(N)-(13)C(alpha) dipolar couplings resulted in structures with large numbers of dipolar restraint violations. As an alternative to direct refinement against measured dipolar couplings we have developed an approach where discrete orientations are calculated for each peptide plane on the basis of the dipolar couplings described above. The orientation which best matches that in initial NMR structures calculated from NOE and dihedral angle restraints exclusively is used to refine further the structures using a new module written for CNS. Modeling studies from four different proteins with diverse structural motifs establishes the utility of the methodology. When applied to experimental data recorded on MBP the precision of the family of structures generated improves from 5.5 to 2.2 A, while the rmsd with respect to the X-ray structure (1dmb) is reduced from 5.1 to 3.3 A.
Asunto(s)
Proteínas Bacterianas/química , Proteínas Bacterianas/metabolismo , Proteínas Portadoras/química , Proteínas Portadoras/metabolismo , Proteínas de Escherichia coli , Resonancia Magnética Nuclear Biomolecular/métodos , Pliegue de Proteína , beta-Ciclodextrinas , Secuencias de Aminoácidos , Simulación por Computador , Cristalografía por Rayos X , Ciclodextrinas/química , Ciclodextrinas/metabolismo , Enlace de Hidrógeno , Modelos Moleculares , Datos de Secuencia Molecular , Peso Molecular , Proteínas de Unión Periplasmáticas , Conformación Proteica , Protones , Sensibilidad y Especificidad , Programas Informáticos , SolucionesRESUMEN
Perdeuteration of all non-exchangeable proton sites can significantly increase the size of proteins and protein complexes for which NMR resonance assignments and structural studies are possible. Backbone 1H, 15N, 13CO, 13C alpha and 13C beta chemical shifts and aliphatic side-chain 13C and 1H(N)/15N chemical shifts for human carbonic anhydrase II (HCA II), a 259 residue 29 kDa metalloenzyme, have been determined using a strategy based on 2D, 3D and 4D heteronuclear NMR experiments, and on perdeuterated 13C/15N-labeled protein. To date, HCA II is one of the largest monomeric proteins studied in detail by high-resolution NMR. Of the backbone resonances, 85% have been assigned using fully protonated 15N and 3C/15N-labeled protein in conjunction with established procedures based on now standard 2D and 3D NMR experiments. HCA II has been perdeuterated both to complete the backbone resonance assignment and to assign the aliphatic side-chain 13C and 1H(N)/15N resonances. The incorporation of 2H into HCA II dramatically decreases the rate of 13C and 1H(N)T2 relaxation. This, in turn, increases the sensitivity of several key 1H/13C/15N triple-resonance correlation experiments. Many otherwise marginal heteronuclear 3D and 4D correlation experiments, which are important to the assignment strategy detailed herein, can now be executed successfully on HCA II. Further analysis suggests that, from the perspective of sensitivity, perdeuteration should allow other proteins with rotational correlation times significantly longer than HCA II (tau c = 11.4 ns) to be studied successfully with these experiments. Two different protocols have been used to characterize the secondary structure of HCA II from backbone chemical-shift data. Secondary structural elements determined in this manner compare favorably with those elements determined from a consensus analysis of the HCA II crystal structure. Finally, having outlined a general strategy for assigning backbone and side-chain resonances in a perdeuterated large protein, we propose a strategy whereby this information can be used to glean more detailed structural information from the partially or fully protonated protein equivalent.
Asunto(s)
Anhidrasas Carbónicas/química , Proteínas/química , Aminoácidos/química , Isótopos de Carbono , Anhidrasas Carbónicas/genética , Deuterio , Escherichia coli/genética , Humanos , Hidrógeno , Estructura Molecular , Isótopos de Nitrógeno , Estructura Secundaria de Proteína , Protones , Proteínas Recombinantes/química , Proteínas Recombinantes/genéticaRESUMEN
The perdeuteration of aliphatic sites in large proteins has been shown to greatly facilitate the process of sequential backbone and side-chain 13C assignments and has also been utilized in obtaining long-range NOE distance restraints for structure calculations. To obtain the maximum information from a 4D 15N/15N-separated NOESY, as many main-chain and side-chain 1HN/15N resonances as possible must be assigned. Traditionally, only backbone amide 1HN/15N resonances are assigned by correlation experiments, whereas slowly exchanging side-chain amide, amino, and guanidino protons are assigned by NOEs to side-chain aliphatic protons. In a perdeuterated protein, however, there is a minimal number of such protons. We have therefore developed several gradient-enhanced and sensitivity-enhanced pulse sequences, containing water-flipback pulses, to provide through-bond correlations of the aliphatic side-chain 1HN/15N resonances to side-chain 13C resonances with high sensitivity: NH2-filtered 2D 1H-15N HSQC(H2N-HSQC), 3D H2N(CO)C gamma/beta and 3D H2N(COC gamma/beta)C beta/alpha for glutamine and asparagine side-chain amide groups; 2D refocused H(N epsilon/zeta)C delta/epsilon and H(N epsilon/zeta C delta/epsilon)C gamma/delta for arginine side-chain amino groups and non-refocused versions for lysine side-chain amino groups; and 2D refocused H(N epsilon)C zeta and nonrefocused H(N epsilon, eta)C zeta for arginine side-chain guanidino groups. These pulse sequences have been applied to perdeuterated 13C-/15N-labeled human carbonic anhydrase II (2H-HCA II). Because more than 95% of all side-chain 13C resonances in 2H-HCA II have already been assigned with the C(CC)(CO)NH experiment, the assignment of the side-chain 1HN/15N resonances has been straightforward using the pulse sequences mentioned above. The importance of assigning these side-chain HN protons has been demonstrated by recent studies in which the calculation of protein global folds was simulated using only 1HN-1HN NOE restraints. In these studies, the inclusion of NOE restraints to side-chain HN protons significantly improved the quality of the global fold that could be determined for a perdeuterated protein [R.A. Venters et al. (1995) J. Am. Chem. Soc., 117, 9592-9593].
Asunto(s)
Aminoácidos , Conformación Proteica , Proteínas/química , Secuencia de Aminoácidos , Arginina , Asparagina , Deuterio , Glutamina , Guanidina , Guanidinas , Humanos , Hidrógeno , Enlace de Hidrógeno , Espectroscopía de Resonancia Magnética/métodos , Isótopos de Nitrógeno , Pliegue de Proteína , Estructura Secundaria de Proteína , Sensibilidad y EspecificidadRESUMEN
The protein human carbonic anhydrase II (HCA II) has been isotopically labeled with 2H, 13C and 15N for high-resolution NMR assignment studies and pulse sequence development. To increase the sensitivity of several key 1H/13C/15N triple-resonance correlation experiments, 2H has been incorporated into HCA II in order to decrease the rates of 13C and 1HN T2 relaxation. NMR quantities of protein with essentially complete aliphatic 2H incorporation have been obtained by growth of E. coli in defined media containing D2O, [1,2-13C2, 99%] sodium acetate, and [15N, 99%] ammonium chloride. Complete aliphatic deuterium enrichment is optimal for 13C and 15N backbone NMR assignment studies, since the 13C and 1HN T2 relaxation times and, therefore, sensitivity are maximized. In addition, complete aliphatic deuteration increases both resolution and sensitivity by eliminating the differential 2H isotopic shift observed for partially deuterated CHnDm moieties.
Asunto(s)
Espectroscopía de Resonancia Magnética/métodos , Proteínas/química , Isótopos de Carbono , Anhidrasas Carbónicas/química , Anhidrasas Carbónicas/genética , Deuterio , Escherichia coli/genética , Humanos , Espectrometría de Masas , Estructura Molecular , Isótopos de NitrógenoRESUMEN
A 3D optimized, refocused HNCA experiment is described. It is demonstrated to yield a dramatic increase in sensitivity when applied to [13C, 15N]-labeled human carbonic anhydrase II, a 29-kDa protein. The reasons for the gain in sensitivity are discussed, and 3 distinct areas for further development are indicated.
Asunto(s)
Anhidrasas Carbónicas/química , Isoenzimas/química , Espectroscopía de Resonancia Magnética/métodos , Isótopos de Carbono , Humanos , Matemática , Isótopos de Nitrógeno , Conformación ProteicaRESUMEN
Uniform double labeling of proteins for NMR studies can be prohibitively expensive, even with an efficient expression and purification scheme, due largely to the high cost of [13C6, 99%]glucose. We demonstrate here that uniformly (greater than 95%) 13C and 15N double-labeled proteins can be prepared for NMR structure/function studies by growing cells in defined media containing sodium [1,2-13C2, 99%]acetate as the sole carbon source and [15N, 99%]ammonium chloride as the sole nitrogen source. In addition, we demonstrate that this labeling scheme can be extended to include uniform carbon isotope labeling to any desired level (below 50%) by utilizing media containing equal amounts of sodium [1-13C, 99%]acetate and sodium [2-13C, 99%]acetate in conjunction with unlabeled sodium acetate. This technique is less labor intensive and more straightforward than labeling using isotope-enriched algal hydrolysates. These labeling schemes have been used to successfully prepare NMR quantities of isotopically enriched human carbonic anhydrase II. The activity and the 1H NMR spectra of the protein labeled by this technique are the same as those obtained from the protein produced from media containing labeled glucose; however, the cost of the sodium [1,2-13C2, 99%]acetate growth media is considerably less than the cost of the [13C6, 99%]glucose growth media. We report here the first published 13C and 15N NMR spectra of human carbonic anhydrase II as an important step leading to the assignment of this 29-kDa zinc metalloenzyme.
Asunto(s)
Acetatos , Anhidrasas Carbónicas/química , Ácido Acético , Isótopos de Carbono , Humanos , Espectroscopía de Resonancia Magnética , Isótopos de NitrógenoRESUMEN
Analysis of biological fluids by proton magnetic resonance spectroscopy is often complicated by dynamic range problems created from the large water resonance. Gel filtration chromatography is found to be a simple and nondestructive method for exchanging D2O for H2O and for removing low molecular weight molecules from both plasma and urine, significantly improving subsequent one- and two-dimensional MRS spectra.
Asunto(s)
Agua Corporal/análisis , Lípidos/análisis , Orina/análisis , Agua Corporal/fisiología , Cromatografía en Gel , Humanos , Lípidos/sangre , Espectroscopía de Resonancia Magnética , ProtonesRESUMEN
A new rhombic EPR signal was recently discovered in the partially reduced type 2 copper-depleted Rhus vernicifera laccase (Reinhammar, B. (1983) J. Inorg. Biochem., in press). The signal originates from one of the type 3 Cu(II) ions that becomes EPR-detectable as a result of the selective reduction of the other copper ion in the exchange-coupled Cu(II)-Cu(II) pair. The 14N and 1H and 63,65Cu electron nuclear double resonance (ENDOR) of this uncoupled Cu(II) now have been collected and represent the first ENDOR measurements of a type 3 copper site. The data indicate that the copper is coordinated by at least three nitrogenous ligands, at least one of which is an imidazole. H/D exchange suggests a nearby H2O or OH-, perhaps as a fourth ligand. A similar EPR signal is seen for CuB of reduced cytochrome c oxidase under turnover conditions. The 14N ENDOR, and, therefore, the structure, of this site corresponds extremely closely to that of the laccase type 3 (Cu(II).
Asunto(s)
Cobre , Complejo IV de Transporte de Electrones/análisis , Oxidorreductasas/análisis , Espectroscopía de Resonancia por Spin del Electrón , LacasaRESUMEN
The effect of compression rate on onset of high-pressure convulsions has been studied in 14 vertebrate species, as well as in 10 mouse strains and 4 rat strains. Compression rate effects were observed in 9 of the 14 species. They appear to be independent of exposure temperature, correlate only very loosely with phylogenetic position, and appear to reflect species-specific compensatory mechanisms grafted onto an underlying convulsion-producing effect of high hydrostatic pressure. Five vertebrate species distributed among three of the four classes tested failed to show a significant degree of compression rate dependence of high-pressure neurological syndrome (HPNS) convulsion thresholds. The implications of this finding for the formulation of hypotheses regarding the biophysical basis for HPNS convulsions has been discussed. Comparison of intrinsic HPNS susceptibility in different species, in the light of these findings, requires that the comparison be made at a common compression rate. Four of the five lower vertebrate species fall consistently into the category showing high HPNS convulsion threshold pressures regardless of the compression rate employed, whereas the two primates and the one carnivore tested equally consistently fall in the low convulsion threshold pressure category. The data suggest a parallel between the degree of brain development and the relative HPNS susceptibility of a given species and contrast with the inverse relations observed during maturation of newborn mice and rats. The results are compared with data for other convulsants and suggest grouping HPNS and pentylenetetrazole seizures as against electroshock, hyperoxic, flurothyl, strychine, or picrotoxin convulsions.