RESUMEN
The sandfly Lutzomyia whitmani (Antunes & Coutinho) is an important vector for cutaneous leishmaniasis throughout much of Brazil, and has recently been shown to consist of more than one mitochondrial lineage. It has frequently been asserted that the degree of adaptation of L. whitmani to human environments varies across its range. As a standardized test of indoor feeding for three geographically distant populations of L. whitmani, catches inside experimental chicken sheds of varying degrees of wall closure (0%, 33%, 67% and 98%) were compared. Each increment in shed closure reduced catches of females (relative to the most open shed) by a similar degree for each population: geometric mean catches dropped by 11-40% with 33% closure, by 41-62% with 67% closure, and by 69-100% with 98% closure. Geometric mean catches of males from the two more northerly populations also decreased with increasing shed closure, by 18% and 22% for 33% closure, 58% and 69% for 67% closure, 91% and 93% for 98% closure. Males from the most southerly population showed significantly different behaviour, with 33% closure causing a 54% increase in geometric mean catch, 67% closure causing a 6% increase, and 98% closure causing a 32% reduction. For this southerly population, sex ratios became more male biased with increasing density in more closed sheds, suggesting aggregation driven by intra-specific communication. Lutzomyia intermedia (Lutz & Neiva) was relatively more likely than L. whitmani to approach baits in the three more closed sheds, rather than the most open shed, offering a behavioural explanation for observed differences in indoor biting rates between the species.
Asunto(s)
Psychodidae , Animales , Conducta Animal , Femenino , Humanos , MasculinoRESUMEN
A randomized ten-year follow-up study involving 91 Chagas patients and 41 uninfected controls was undertaken to determine the effectiveness of nitroderivative therapy. Anti-Trypanosoma cruzi antibodies were consistently lower one year after treatment than 10 years thereafter (P < 0.001). The blood of all treated and 93.7% of untreated Chagas patients yielded polymerase chain reaction (PCR) product from probes annealing to T. cruzi nuclear DNA, indicating active infection. Competitive PCR showed means +/- standard deviations of 20.1+/-22.6 T. cruzi/ml of blood from untreated and 13.8+/-14.9 from treated Chagas patients, but the differences between means were not statistically significant (P > 0.05). Electrocardiograms recorded a gamut of alterations several-fold more frequent in Chagas patients, regardless of treatment, than in uninfected controls (P < 0.001). These results show that nitroderivative therapy for T. cruzi infections is unsatisfactory and cannot be recommended since it fails to eradicate the parasite or change the progression of heart disease in chronic Chagas patients.
Asunto(s)
Anticuerpos Antiprotozoarios/sangre , Cardiomiopatía Chagásica/tratamiento farmacológico , Tripanocidas/uso terapéutico , Trypanosoma cruzi/inmunología , Adulto , Animales , Estudios de Casos y Controles , Enfermedad Crónica , Cartilla de ADN , Progresión de la Enfermedad , Electrocardiografía , Ensayo de Inmunoadsorción Enzimática , Técnica del Anticuerpo Fluorescente , Estudios de Seguimiento , Pruebas de Hemaglutinación , Humanos , Masculino , Persona de Mediana Edad , Nifurtimox/uso terapéutico , Nitroimidazoles/uso terapéutico , Reacción en Cadena de la Polimerasa , Resultado del TratamientoAsunto(s)
Enfermedad de Chagas/diagnóstico , Leishmania braziliensis , Leishmania infantum , Leishmaniasis Cutánea/diagnóstico , Leishmaniasis Visceral/diagnóstico , Animales , Anticuerpos Antiprotozoarios/sangre , Humanos , Leishmania braziliensis/inmunología , Leishmania infantum/inmunología , Sensibilidad y Especificidad , Pruebas Serológicas/métodos , Trypanosoma cruzi/inmunologíaRESUMEN
We have detected antibodies, in the sera of Chagas disease, Kala-azar and Mucocutaneous leishmaniasis patients, that bind multiple antigens shared between the three causative agents. The Chagas disease sera showed 98 to 100% positive results by ELISA when the Leishmania braziliensis and Leishmania chagasi antigens were used, respectively. The Kala-azar sera showed 100% positive results with Trypanosoma cruzi or L. braziliensis antigens by immunofluorescence assays. The antibodies in the sera of Mucocutaneous leishmaniasis patients showed 100% positive results by ELISA assays with T. cruzi or L. chagasi antigens. Furthermore, the direct agglutination of L. chagasi promastigotes showed that 95% of Kala-azar and 35% of Mucocutaneous leishmaniasis sera agglutinated the parasite in dilutions above 1:512. In contrast, 15% of Chagas sera agglutinated the parasite in dilutions 1:16 and below. Western blot analysis showed that the Chagas sera that formed at least 24 bands with the T. cruzi also formed 13 bands with the L. chagasi and 17 bands with the L. braziliensis. The Kala-azar sera that recognized at least 29 bands with the homologous antigen also formed 14 bands with the T. cruzi and 10 bands with the L. braziliensis antigens. Finally, the Mucocutaneous leishmaniasis sera that formed at least 17 bands with the homologous antigen also formed 10 bands with the T. cruzi and four bands with the L. chagasi antigens. These results indicate the presence of common antigenic determinants in several protozoal proteins and, therefore, explain the serologic cross-reactions reported here.
Asunto(s)
Anticuerpos Antiprotozoarios/inmunología , Enfermedad de Chagas/inmunología , Leishmaniasis Mucocutánea/inmunología , Leishmaniasis Visceral/inmunología , Animales , Anticuerpos Antiprotozoarios/sangre , Enfermedad de Chagas/sangre , Reacciones Cruzadas , Humanos , Leishmania braziliensis/inmunología , Leishmania infantum/inmunología , Leishmaniasis Mucocutánea/sangre , Leishmaniasis Visceral/sangre , Trypanosoma cruzi/inmunologíaRESUMEN
Os autores apresentam um caso de leishmaniose tegumentar americana causada por Leishmânia viannia brasiliensis que de um grupo de seis pacientes tratados com a associaçäo glucantime e alopurinol desenvolveu quadro de nefrite intersticial aguda acompanhado de exantema cutâneo. Questionam se näo houve potencializaçäo na interaçäo das drogas com relaçäo ao efeito desejável
Asunto(s)
Persona de Mediana Edad , Humanos , Masculino , Lesión Renal Aguda/inducido químicamente , Alopurinol/efectos adversos , Quimioterapia Combinada , Leishmaniasis Mucocutánea/complicaciones , Hidroclorotiazida/uso terapéutico , Leishmaniasis Mucocutánea/tratamiento farmacológico , Metildopa/uso terapéutico , Penicilina G Procaína/uso terapéuticoRESUMEN
Fourteen marmosets (Callithrix penicillata) were inoculated intradermally with promastigotes and/or amastigotes of Leishmania (Viannia) braziliensis (L. (V) b.) strains MHOM/BR/83/LTB-300 MHOM/BR/85/LTB-12 MHOM/BR/81/LTB-179 and MHOM/BR/82/LTB-250. The evolution of subsequent lesions was studied for 15 to 75 weeks post-inoculation (PI). All but 3 of the L. (V) b. injected marmosets developed a cutaneous lesion at the point of inoculation after 3 to 9 weeks, characterized by the appearance of subcutaneous nodules containing parasites. Parasites were isolated by culture (Difco Blood Agar) from all 11 positive animals. The maximum size of the lesions was variable and ranged between 37 mm2 to 107 mm2. Ulceration of primary nodules became evident after 3 to 12 weeks in all infected marmosets, but was faster and larger in 5 of the 11 animals. The active lesions persisted in 9 out of 11 Callithrix until the end of the observation period, which varied from 15-75 weeks. In 3 animals spontaneous healing of their lesions (13 to 25 weeks, PI) was observed but with cryptic parasitism. In another 2 infected animals there was regression followed by reactivation of the cutaneous lesions. The appearance of smaller satellite lesions adjacent to primary ones, as well as metastatic lesions to the ear lobes, were documented in 2 animals. Promastigotes of L. (Leishmania) amazonensis (L. (L) a.) MHOM/BR/77/LTB-16 were inoculated in 1 marmoset. This animal remained chronically infected for 6 months and the lesion developed in a similar manner to L. (V) b. infected marmosets. No significant differences in clinical and parasitological behaviour were observed between promastigote or amastigote derived infections of the 2 species.(ABSTRACT TRUNCATED AT 250 WORDS)
Asunto(s)
Leishmania braziliensis/fisiología , Leishmaniasis Cutánea/parasitología , Animales , Anticuerpos Antiprotozoarios/análisis , Callithrix , Modelos Animales de Enfermedad , Leishmania braziliensis/inmunología , Leishmaniasis Cutánea/inmunologíaAsunto(s)
Alopurinol/uso terapéutico , Antiprotozoarios/uso terapéutico , Leishmaniasis Mucocutánea/tratamiento farmacológico , Meglumina/uso terapéutico , Compuestos Organometálicos/uso terapéutico , Adulto , Anciano , Quimioterapia Combinada , Humanos , Antimoniato de Meglumina , Persona de Mediana EdadRESUMEN
The characterization and identification to species and subspecies of 20 stocks of Leishmania isolated from the region of Três Braços, Bahia, Brazil, are described: 17 stocks were from patients and three from dogs. The following techniques were used (i) biological (growth in culture, hamster tissues and phlebotomine gut), (ii) biochemical (isoenzyme and kinetoplast DNA analysis) and (iii) immunological (using monoclonal antibodies). All except two stocks belong to the L. braziliensis complex. One of these two corresponded to L. mexicana amazonensis but the other, while clearly in the mexicana complex, showed slight differences from the L. mexicana amazonensis reference strain on isoenzyme analysis. Two stocks from different lesions in the same patient and with different growth characteristics in hamster tissues were both identified as L. braziliensis braziliensis. All the fully characterized stocks of the L. braziliensis complex were identified as L. braziliensis braziliensis. L. braziliensis guyanensis was not identified. Dog and human stocks of L. braziliensis braziliensis were indistinguishable. From these findings and other evidence, L. braziliensis braziliensis seems to be the predominant species transmitted in Três Braços.
Asunto(s)
Enfermedades de los Perros/parasitología , Leishmania braziliensis/aislamiento & purificación , Leishmania/aislamiento & purificación , Leishmaniasis Mucocutánea/parasitología , Animales , Anticuerpos Monoclonales/inmunología , Brasil , Cricetinae , ADN Mitocondrial/análisis , Perros , Electroforesis en Gel de Almidón , Técnica del Anticuerpo Fluorescente , Humanos , Isoenzimas/análisis , Leishmania braziliensis/análisis , Leishmania braziliensis/enzimología , Leishmania mexicana/análisis , Leishmania mexicana/enzimología , Leishmania mexicana/aislamiento & purificación , Leishmaniasis/parasitología , Leishmaniasis/veterinaria , Leishmaniasis Mucocutánea/veterinariaAsunto(s)
Leishmania/clasificación , Leishmaniasis Mucocutánea/parasitología , Animales , Brasil , Cricetinae , HumanosAsunto(s)
Infección de Laboratorio/etiología , Leishmaniasis Mucocutánea/etiología , Adulto , Animales , Cricetinae , Humanos , MasculinoRESUMEN
Estudo de um caso de xenodiagnostico em paciente com doenca de Chagas agudo. A suscetibilidade do D. maximus para a cepa de Barreiras do T. cruzi foi estudada com 100 ninfas do terceiro estagio alimentadas em uma paciente com Doenca de Chagas agudo. A taxa de infeccao nestes triatomineos foi tao alta que nao foi possivel identificar um grupo refratario para a criacao