Asunto(s)
Adalimumab/efectos adversos , Erupciones por Medicamentos/etiología , Infliximab/efectos adversos , Piel/patología , Factor de Necrosis Tumoral alfa/antagonistas & inhibidores , Adulto , Enfermedad de Crohn/tratamiento farmacológico , Erupciones por Medicamentos/patología , Humanos , Linfocitos , Masculino , Psoriasis/tratamiento farmacológicoRESUMEN
Polymers of hyaluronic acid (Hyal) esters exhibit good tissue compatibility and are available in various geometrical configurations. These properties can be exploited for the design of innovative bioartificial liver support devices (BALSDs) using primary hepatocytes. In this paper, we report a preliminary investigation of the polymer feasibility of the ethyl and the benzyl Hyal ester in the form of films and non-woven fabrics for the in vitro culture of primary rat hepatocytes. Cell function was evaluated daily in Petri dishes with respect to the rate of ammonia elimination (AER) and urea synthesis (USR). Cells cultured in non-woven fabrics of the ethyl ester of Hyal (HYAFF7nw) exhibited an initial AER about 32% lower and synthesised urea 33% faster than that of cells on collagen films. After a week in culture, cells on collagen films retained only a minor fraction of their initial rates. Cells cultured in non-woven fabrics of HYAFF7nw retained about 62 and 44% of their initial AER and USR, respectively, and exhibited an AER approximately equal to and a USR 3.6 times greater than those of cells adherent to collagen. These results suggest that non-woven fabrics of HYAFF7nw are promising substrata for hepatocyte culture in BALSDs.
Asunto(s)
Materiales Biocompatibles , Técnicas de Cultivo de Célula/métodos , Hepatocitos/citología , Hepatocitos/metabolismo , Amoníaco/metabolismo , Animales , Adhesión Celular , Células Cultivadas , Ácido Hialurónico/análogos & derivados , Ensayo de Materiales , Microscopía Electrónica de Rastreo , Polímeros , Ratas , Propiedades de Superficie , Urea/metabolismoRESUMEN
We report the construction of a tiling path of around 650 clones covering more than 99% of human chromosome 14. Clone overlap information to assemble the map was derived by comparing fully sequenced clones with a database of clone end sequences (sequence tag connector strategy). We selected homogeneously distributed seed points using an auxiliary high-resolution radiation hybrid map comprising 1,895 distinct positions. The high long-range continuity and low redundancy of the tiling path indicates that the sequence tag connector approach compares favourably with alternative mapping strategies.
Asunto(s)
Cromosomas Humanos Par 14 , Cromosomas Artificiales Bacterianos , Clonación Molecular , Escherichia coli , Humanos , Mapeo Físico de Cromosoma , Mapeo de Híbrido por Radiación , Lugares Marcados de SecuenciaRESUMEN
The generation of sequencing data for the hemiascomycetous yeast random sequence tag project was performed using the procedures established at GENOSCOPE. These procedures include a series of protocols for the sequencing reactions, using infra-red labelled primers, performed on both ends of the plasmid inserts in the same reaction tube, and their analysis on automated DNA sequencers. They also include a package of computer programs aimed at detecting potential assignation errors, selecting good quality sequences and estimating their useful length.