RESUMEN
Embryonic stem cells (ESCs) are self-renewing and pluripotent. In recent years, factors that control pluripotency, mostly nuclear, have been identified. To identify non-nuclear regulators of ESCs, we screened an endogenously labeled fluorescent fusion-protein library in mouse ESCs. One of the more compelling hits was the cell-cycle-associated protein 1 (CAPRIN1). CAPRIN1 knockout had little effect in ESCs, but it significantly altered differentiation and gene expression programs. Using RIP-seq and SLAM-seq, we found that CAPRIN1 associates with, and promotes the degradation of, thousands of RNA transcripts. CAPRIN1 interactome identified XRN2 as the likely ribonuclease. Upon early ESC differentiation, XRN2 is located in the nucleus and colocalizes with CAPRIN1 in small RNA granules in a CAPRIN1-dependent manner. We propose that CAPRIN1 regulates an RNA degradation pathway operating during early ESC differentiation, thus eliminating undesired spuriously transcribed transcripts in ESCs.
Asunto(s)
Proteínas de Ciclo Celular , Exorribonucleasas , Células Madre Embrionarias de Ratones , Animales , Ratones , Ciclo Celular , Proteínas de Ciclo Celular/metabolismo , Diferenciación Celular , Estabilidad del ARN , Exorribonucleasas/metabolismoRESUMEN
In this issue of Cell Stem Cell, Di Stefano et al. (2019) identify the RNA helicase DDX6 as a regulator of stem cell pluripotency and differentiation. DDX6 depletion leads to P-body disruption, which, in turn, inhibits or promotes stem and progenitor cell differentiation, depending on their cellular context.