NP3 MS Workflow: An Open-Source Software System to Empower Natural Product-Based Drug Discovery Using Untargeted Metabolomics.

Natural products (or specialized metabolites) are historically the main source of new drugs. However, the current drug discovery pipelines require miniaturization and speeds that are incompatible with traditional natural product research methods, especially in the early stages of the research. This article introduces the NP3 MS Workflow, a robust open-source software system for liquid chromatography-tandem mass spectrometry (LC-MS/MS) untargeted metabolomic data processing and analysis, designed to rank bioactive natural products directly from complex mixtures of compounds, such as bioactive biota samples. NP3 MS Workflow allows minimal user intervention as well as customization of each step of LC-MS/MS data processing, with diagnostic statistics to allow interpretation and optimization of LC-MS/MS data processing by the user. NP3 MS Workflow adds improved computing of the MS2 spectra in an LC-MS/MS data set and provides tools for automatic [M + H]+ ion deconvolution using fragmentation rules; chemical structural annotation against MS2 databases; and relative quantification of the precursor ions for bioactivity correlation scoring. The software will be presented with case studies and comparisons with equivalent tools currently available. NP3 MS Workflow shows a robust and useful approach to select bioactive natural products from complex mixtures, improving the set of tools available for untargeted metabolomics. It can be easily integrated into natural product-based drug-discovery pipelines and to other fields of research at the interface of chemistry and biology.

Isolated Benign Unilateral Episodic Mydriasis in Relation to Anxiety: A Case Report.

Benign episodic mydriasis is a form of anisocoria that is characterised by isolated, episodic, and sporadic mydriasis, in the absence of accompanying neurological or systemic signs or symptoms. The diagnosis is established after other causes of anisocoria have been excluded. The purpose of this case report is to describe a case of benign episodic mydriasis of her right pupil in a 23-year-old female patient. The episodes lasted for 5-10 minutes and occurred spontaneously, without any concomitant symptoms or precipitating factors. The frequency of the episodes was variable, but she reported that it worsened in periods when she felt more mentally stressed or anxious. Contact with any kind of medications, homoeopathic drugs, industrial agents, or any other kind of chemical compounds was excluded, and the remaining neurological and systemic examination was unremarkable. Blood tests were taken and imaging was performed, which ruled out a structural lesion. A diagnosis of isolated benign episodic mydriasis was established. Unlike the typical presentation of the condition, the episodes did not occur in association with any form of migraine or headache. Given the benign nature of the condition, no treatment was instituted. After excluding potentially severe causes, and reassuring the patient, the frequency of the episodes decreased.

Atypical case of acute posterior multifocal placoid pigment epitheliopathy with intraretinal fluid.

Acute posterior multifocal placoid pigment epitheliopathy is a rare inflammatory chorioretinopathy, classified as a white dot syndrome, in which ischaemia of the choriocapillaris leads to atrophy of the external retinal layers, including the retinal pigment epithelium.A male patient in his 20s presented with sudden severe loss of vision in the left eye. Funduscopy revealed with yellow placoid lesions in the macula and near periphery. Spectral-domain optical coherence tomography and fluorescein angiography revealed the presence of central intraretinal fluid in the left eye and multiple areas of macular ischaemia bilaterally. Treatment with oral corticosteroids was initiated, and the anatomical changes, including the intraretinal fluid, improved steadily over the following weeks.Although rare, the presence of subretinal or intraretinal fluid should not decrease the suspicion of acute posterior multifocal placoid pigment epitheliopathy. Reabsorption of the fluid is usually accompanied by the improvement of the remaining anatomical changes and the visual function.

Medidas abrangente e reduzida de comportamento de suporte do supervisor à família / Comprehensive and short-form measures of family supportive supervisor behaviors / Medidas integral y reducida de comportamiento de apoyo al supervisor familiar

O suporte do supervisor à família conceitua-se como comportamentos dos supervisores voltados a apoiar os papéis familiares dos funcionários. O presente estudo objetivou obter evidências de validade da Medida de Comportamento Suporte do Supervisor à Família, nas versões abrangente e reduzida, em amostras brasileiras. Participaram desta pesquisa 503 trabalhadores, de ambos os sexos. As análises fatoriais confirmatórias evidenciaram que a versão abrangente ficou composta por 14 itens, divididos em quatro fatores de primeira ordem (emocional, instrumental, modelo de papéis e gestão criativa) e um fator de segunda ordem. A versão reduzida unifatorial compôs-se por quatro itens. Ambas as versões apresentaram bons índices de consistência interna. Os instrumentos correlacionaram-se positivamente com o enriquecimento trabalho-família, suporte do supervisor e satisfação no trabalho, e negativamente com o conflito trabalho-família e intenção de rotatividade. Concluiu-se que os instrumentos apresentaram propriedades psicométricas que recomendam seu uso em investigações futuras.(AU)

The concept of a family-supportive supervisor refers to supervisors' behaviors aimed at supporting employees' family roles. This study aimed to obtain evidence of validity of the Measure of Family Supportive Supervisor Behaviors, both in its comprehensive and abbreviated versions, using Brazilian samples. A total of 503 workers of both sexes participated in this research. Confirmatory factor analyses showed that the comprehensive version consisted of 14 items, grouped into four first-order factors (emotional, instrumental, role model and creative management), all contributing to a second-order factor. The unifactorial reduced version consisted of four items. Both versions showed good internal consistency indices. The instruments exhibited positive correlations with work-family enrichment, supervisor support, and job satisfaction while demonstrating negative correlations with work-family conflict and turnover intention. It was concluded that the instruments presented psychometric properties that recommend their use in future research.(AU)

El apoyo del supervisor a la familia se define a las acciones de los supervisores dirigidos para respaldar los roles familiares de los empleados. El presente estudio tuvo como objetivo obtener evidencias de validez de la Medida del Comportamiento de Apoyo del Supervisor a la Familia, en las versiones extensa y breve, en muestras brasileñas. En esta investigación participaron 503 trabajadores de ambos sexos. Los análisis factoriales confirmatorios revelaron que la versión extensa quedó compuesta por 14 ítems, divididos en cuatro factores de primer orden (emocional, instrumental, modelo a seguir y gestión creativa) y un factor de segundo orden. La versión breve unifactorial constaba de cuatro ítems. Ambas versiones mostraron buenos índices de consistencia interna. Los instrumentos se correlacionaron positivamente con el enriquecimiento trabajo-familia, el apoyo del supervisor y la satisfacción laboral, y negativamente con el conflicto trabajo-familia y la intención de rotación. Se concluye que los instrumentos presentan propiedades psicométricas que respaldan su uso en futuras investigaciones.(AU)

Towards FAIRification of sensitive and fragmented rare disease patient data: challenges and solutions in European reference network registries.

INTRODUCTION: Rare disease patient data are typically sensitive, present in multiple registries controlled by different custodians, and non-interoperable. Making these data Findable, Accessible, Interoperable, and Reusable (FAIR) for humans and machines at source enables federated discovery and analysis across data custodians. This facilitates accurate diagnosis, optimal clinical management, and personalised treatments. In Europe, twenty-four European Reference Networks (ERNs) work on rare disease registries in different clinical domains. The process and the implementation choices for making data FAIR ('FAIRification') differ among ERN registries. For example, registries use different software systems and are subject to different legal regulations. To support the ERNs in making informed decisions and to harmonise FAIRification, the FAIRification steward team was established to work as liaisons between ERNs and researchers from the European Joint Programme on Rare Diseases. RESULTS: The FAIRification steward team inventoried the FAIRification challenges of the ERN registries and proposed solutions collectively with involved stakeholders to address them. Ninety-eight FAIRification challenges from 24 ERNs' registries were collected and categorised into "training" (31), "community" (9), "modelling" (12), "implementation" (26), and "legal" (20). After curating and aggregating highly similar challenges, 41 unique FAIRification challenges remained. The two categories with the most challenges were "training" (15) and "implementation" (9), followed by "community" (7), and then "modelling" (5) and "legal" (5). To address all challenges, eleven types of solutions were proposed. Among them, the provision of guidelines and the organisation of training activities resolved the "training" challenges, which ranged from less-technical "coffee-rounds" to technical workshops, from informal FAIR Games to formal hackathons. Obtaining implementation support from technical experts was the solution type for tackling the "implementation" challenges. CONCLUSION: This work shows that a dedicated team of FAIR data stewards is an asset for harmonising the various processes of making data FAIR in a large organisation with multiple stakeholders. Additionally, multi-levelled training activities are required to accommodate the diverse needs of the ERNs. Finally, the lessons learned from the experience of the FAIRification steward team described in this paper may help to increase FAIR awareness and provide insights into FAIRification challenges and solutions of rare disease registries.

Domain-Specific Common Data Elements for Rare Disease Registration: Conceptual Approach of a European Joint Initiative Toward Semantic Interoperability in Rare Disease Research.

BACKGROUND: With hundreds of registries across Europe, rare diseases (RDs) suffer from fragmented knowledge, expertise, and research. A joint initiative of the European Commission Joint Research Center and its European Platform on Rare Disease Registration (EU RD Platform), the European Reference Networks (ERNs), and the European Joint Programme on Rare Diseases (EJP RD) was launched in 2020. The purpose was to extend the set of common data elements (CDEs) for RD registration by defining domain-specific CDEs (DCDEs). OBJECTIVE: This study aims to introduce and assess the feasibility of the concept of a joint initiative that unites the efforts of the European Platform on Rare Disease Registration Platform, ERNs, and European Joint Programme on Rare Diseases toward extending RD CDEs, aiming to improve the semantic interoperability of RD registries and enhance the quality of RD research. METHODS: A joint conference was conducted in December 2020. All 24 ERNs were invited. Before the conference, a survey was communicated to all ERNs, proposing 18 medical domains and requesting them to identify highly relevant choices. After the conference, a 3-phase plan for defining and modeling DCDEs was drafted. Expected outcomes included harmonized lists of DCDEs. RESULTS: All ERNs attended the conference. The survey results indicated that genetic, congenital, pediatric, and cancer were the most overlapping domains. Accordingly, the proposed list was reorganized into 10 domain groups and recommunicated to all ERNs, aiming at a smaller number of domains. CONCLUSIONS: The approach described for defining DCDEs appears to be feasible. However, it remains dynamic and should be repeated regularly based on arising research needs.

Production of Epoxyketone Peptide-Based Proteasome Inhibitors by Streptomyces sp. BRA-346: Regulation and Biosynthesis.

Streptomyces sp. BRA-346 is an Actinobacteria isolated from the Brazilian endemic tunicate Euherdmania sp. We have reported that this strain produces epoxyketone peptides, as dihydroeponemycin (DHE) and structurally related analogs. This cocktail of epoxyketone peptides inhibits the proteasome chymotrypsin-like activity and shows high cytotoxicity to glioma cells. However, low yields and poor reproducibility of epoxyketone peptides production by BRA-346 under laboratory cultivation have limited the isolation of epoxyketone peptides for additional studies. Here, we evaluated several cultivation methods using different culture media and chemical elicitors to increase the repertoire of peptide epoxyketone production by this bacterium. Furthermore, BRA-346 genome was sequenced, revealing its broad genetic potential, which is mostly hidden under laboratory conditions. By using specific growth conditions, we were able to evidence different classes of secondary metabolites produced by BRA-346. In addition, by combining genome mining with untargeted metabolomics, we could link the metabolites produced by BRA-346 to its genetic capacity and potential regulators. A single biosynthetic gene cluster (BGC) was related to the production of the target epoxyketone peptides by BRA-346. The candidate BGC displays conserved biosynthetic enzymes with the reported eponemycin (EPN) and TMC-86A (TMC) BGCs. The core of the putative epoxyketone peptide BGC (ORFs A-L), in which ORF A is a LuxR-like transcription factor, was cloned into a heterologous host. The recombinant organism was capable to produce TMC and EPN natural products, along with the biosynthetic intermediates DH-TMC and DHE, and additional congeners. A phylogenetic analysis of the epn/tmc BGC revealed related BGCs in public databases. Most of them carry a proteasome beta-subunit, however, lacking an assigned specialized metabolite. The retrieved BGCs also display a diversity of regulatory genes and TTA codons, indicating tight regulation of this BGC at the transcription and translational levels. These results demonstrate the plasticity of the epn/tmc BGC of BRA-346 in producing epoxyketone peptides and the feasibility of their production in a heterologous host. This work also highlights the capacity of BRA-346 to tightly regulate its secondary metabolism and shed light on how to awake silent gene clusters of Streptomyces sp. BRA-346 to allow the production of pharmacologically important biosynthetic products.

Estudo de caso intrínseco de um recém-nascido prematuro: procedimentos dolorosos / Intrinsic case study of a premature newborn: painful procedures / Estudio de caso intrínseco de un recién nacido prematuro: procedimientos dolorosos

Objetivo: identificar e descrever os procedimentos invasivos/dolorosos e as medidas analgésicas aplicadas em um recém-nascido prematuro extremo durante o período de internação em uma Unidade de Terapia Intensiva Neonatal. Método: trata-se de uma pesquisa observacional, tipo estudo de caso de caráter intrínseco, quantitativo, descritivo e analítico. Os dados foram coletados do prontuário de um lactente que nasceu com idade gestacional de 26 semanas e com seguimento no Ambulatório Canguru, de um hospital escola, do interior do Estado do Paraná. Resultados: o recém-nascido foi submetido a 368 procedimentos invasivos e dolorosos durante todo o período de internação (73 dias), sendo a aspiração de vias aéreas o procedimento prevalente realizado 217 vezes. Destes procedimentos, 64% foram realizados com o uso de analgesia e 36% sem medidas analgésicas. Considerações Finais: os procedimentos dolorosos aos quais o RN foi submetido, durante o período de internação foram expressivos. Por meio de uma ação efetiva e segura foi prescrito o método farmacológico para minimizar a dor.

Objective: identifying and describing the invasive/painful procedures and the analgesic measures applied inan extremely premature newborn baby during the period of hospitalization in a Neonatal Intensive Care Unit. Method: it is an observational search, a case studyof an intrinsic, quantitative, descriptive and analytical character. The data were collected from the medical records of a baby who was born at the gestational age of 26 weeks, with follow-up in Medical Clinic Canguru, which belongs to a teaching hospitalin the countryside of Paraná State. Results: the newborn baby was submitted to 368 invasive andpainful procedures during the whole period of hospitalization (73 days), being airway aspiration the prevalent procedure, performed 217 times. Of these procedures, 64% were carried out with the use of analgesia and 36% without any analgesic measures. Final Considerations: the painful procedures to which the newborn baby was submitted during the hospitalization period were expressive. By means of an effective andsafe action, the pharmacological method was prescribed in order to minimize pain.

Objetivo: identificar y describir los procedimientos invasivos/dolorosos y las medidas analgésicas aplicadas a un recién nacido extremadamente prematuro durante el período de hospitalización en una Unidad de Cuidados Intensivos Neonatales. Método: se trata de una investigación observacional, tipo estudio de caso, de carácter intrínseco, cuantitativo, descriptivo y analítico. Los datos fueron recolectados de la historia clínica de un niño que nació con edad gestacional de 26 semanas y seguido en el Ambulatorio Canguro de un hospital escuela del interior del estado de Paraná. Resultados: el recién nacido fue sometido a 368 procedimientos invasivos y dolorososdurante todo el período de hospitalización (73 días), siendo la aspiración de las vías aéreas el procedimiento predominante realizado 217 veces. De estos procedimientos, el 64% se realizaron con el uso de analgesia y el 36% sin medidas analgésicas. Consideraciones finales: los procedimientos dolorosos a los que fue sometido el RN durante el período de hospitalización fueron significativos. A través de una acción eficaz y segura, se prescribió el método farmacológico para minimizar el dolor.

Semantic modelling of common data elements for rare disease registries, and a prototype workflow for their deployment over registry data.

BACKGROUND: The European Platform on Rare Disease Registration (EU RD Platform) aims to address the fragmentation of European rare disease (RD) patient data, scattered among hundreds of independent and non-coordinating registries, by establishing standards for integration and interoperability. The first practical output of this effort was a set of 16 Common Data Elements (CDEs) that should be implemented by all RD registries. Interoperability, however, requires decisions beyond data elements - including data models, formats, and semantics. Within the European Joint Programme on Rare Diseases (EJP RD), we aim to further the goals of the EU RD Platform by generating reusable RD semantic model templates that follow the FAIR Data Principles. RESULTS: Through a team-based iterative approach, we created semantically grounded models to represent each of the CDEs, using the SemanticScience Integrated Ontology as the core framework for representing the entities and their relationships. Within that framework, we mapped the concepts represented in the CDEs, and their possible values, into domain ontologies such as the Orphanet Rare Disease Ontology, Human Phenotype Ontology and National Cancer Institute Thesaurus. Finally, we created an exemplar, reusable ETL pipeline that we will be deploying over these non-coordinating data repositories to assist them in creating model-compliant FAIR data without requiring site-specific coding nor expertise in Linked Data or FAIR. CONCLUSIONS: Within the EJP RD project, we determined that creating reusable, expert-designed templates reduced or eliminated the requirement for our participating biomedical domain experts and rare disease data hosts to understand OWL semantics. This enabled them to publish highly expressive FAIR data using tools and approaches that were already familiar to them.

Condições associadas ao agravamento da COVID-19 em crianças e adolescentes: protocolo de revisão de escopo / Conditions associated with the worsening of COVID-19 in children and adolescents: scoping review protocol / Condiciones asociadas al agravamiento de la COVID-19 en niños y adolescentes: protocolo de scoping review

OBJETIVO: Mapear o conhecimento científico disponível sobre as condições associadas ao agravamento do quadro da COVID-19 nas populações infantil e adolescente. MÉTODO: Protocolo de scoping review engendrada com base no desenho metodológico do Instituto Joanna Briggs. As bases de dados selecionadas foram: MEDLINE via PubMed, Scopus, Web of Science, Cumulative Index to Nursing and Allied Health Literature (CINAHL), LILACS via Biblioteca Virtual em Saúde (BVS), e Google Acadêmico. A busca dos artigos será realizada por dois pesquisadores independentes. A recuperação das referências se dará em três passos, conforme diretrizes preconizadas pelo Instituto Joanna Briggs. Serão empregados os descritores do Medical Subject Headings (MeSH) e os Descritores em Ciências da Saúde (DeCS). Para extração dos resultados, utilizar-se-á da ferramenta de mapeamento sugerida pelo referencial adotado. Os resultados serão apresentados de acordo com o checklist do Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews (PRISMA-ScR).

OBJECTIVE: To map the available scientific knowledge about the conditions associated with the worsening of the COVID-19 situation in children and adolescents. METHOD: This is a protocol of a scoping review based on the Joanna Briggs Institute methodology. The following databases were chosen: MEDLINE via PubMed, Scopus, Web of Science, Cumulative Index to Nursing and Allied Health Literature (CINAHL), LILACS via Virtual Health Library (BVS), and Google Scholar. Two independent reviewers will carry out the search. The retrieval of references will take place in three steps according to the Joanna Briggs Institute recommendations. Medical Subject Headings (MeSH) descriptors and Health Sciences Descriptors (DeCS) will be used. The mapping tool suggested by the adopted reference will be used to extract the results. The results will be presented according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews (PRISMA-ScR).

OBEJTIVO: Mapear el conocimiento científico disponible sobre las condiciones asociadas al empeoramiento de la situación del COVID-19 en niños y adolescentes. MÉTODO: Este es un protocolo de una scoping review basado en la metodología del Joanna Briggs Institute. Se eligieron las siguientes bases de datos: MEDLINE a través de PubMed, Scopus, Web of Science, Cumulative Index to Nursing and Allied Health Literature (CINAHL), LILACS a través de la Biblioteca Virtual en Salud (BVS) y Google Scholar. Dos revisores independientes realizarán la búsqueda. La recuperación de referencias se realizará en tres pasos según las recomendaciones del Joanna Briggs Institute. Se utilizará los Medical Subject Heading Terms (MeSH) y los Descriptores de Ciencias de la Salud (DeSC). La herramienta de mapeo sugerida por la referencia adoptada se utilizará para extraer los resultados. Los resultados se presentarán de acuerdo con los elementos del Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews (PRISMA-Scr).

Whipple's disease orbitopathy: case report and review of literature.

A 59-year-old female patient was diagnosed with Whipple's disease (WD) after several months of constitutional complaints and adenopathies that were initially misinterpreted as sarcoidosis. Initial treatment included doxycycline, hydroxychloroquine and prednisolone, which was suspended due to long-term clinical stability. Four months after prednisolone suspension, the patient presented with right periorbital oedema and erythema. Ophthalmological examination revealed restricted eye movements. A computed tomography (CT) scan demonstrated signs of myositis. The patient was treated with anti-inflammatory and antibiotic drugs, that induced remission of the orbitopathy. During the following two years, she presented three relapses, affecting both the right or the left eyes. The last episode was also associated with systemic corticosteroid tapering. Orbitopathy is a rare form of WD presentation and the diagnosis of this condition may be challenging. As the clinical spectrum may range from an incidentaloma to a severe compressive neuropathy, proper recognition and management of WD orbitopathy is essential.

Effects of two modalities of noninvasive ventilation on breathing pattern of very low birth weight preterm infants immediately after extubation: a quasi-experimental study.

AIM: The primary objective of this study was to investigate the effects of two modalities of noninvasive ventilation, continuous positive airway pressure-CPAP and non-synchronized nasal intermittent positive pressure ventilation-nsNIPPV, on breathing pattern of very low birth weight preterm infants immediately after extubation. METHODS: It was conducted a quasi-experimental study at a public university hospital. Infants with gestacional age ≤32 weeks and birth weight ≤1,500 g were randomized into the sequences, prior extubation: CPAP - nsNIPPV (1) or nsNIPPV - CPAP (2). Each preterm infant was studied for a period of 60 min in each ventilatory mode. Respiratory inductive plethysmography was used to assess breathing pattern. Inferential analysis was performed by repeated measures ANOVA or Friedman test. RESULTS: Eleven preterm infants were studied and a total of 7,564 respiratory cycles were analyzed. No significant differences were observed in any of the comparisons made for any of the breathing pattern variables (p > .05). CONCLUSIONS: There was no significant difference on breathing pattern between CPAP and nsNIPPV of preterm infants after extubation.

Development and validation of a LC-PDA method for methylphenidate analysis in sewage.

Methylphenidate (MPH) is an important emerging pollutant found in effluents and wastewater. Thus, we aimed to develop and validate a method for detection and quantitation of MPH residues in sewage through high performance liquid chromatography coupled with photodiode array detector (LC-PDA). Here we describe a selective, accurate, precise, and valid method for determination of MPH in sewage with a total running time of 10 min, with limits of detection and quantification of 0.27 and 0.92 µg/mL, respectively. MPH retention peak was observed at 5 min. The method was applied to MPH analysis in a sewage sample pretreated with solid phase extraction, obtaining a result of 2.8 µg/L of MPH. Thus, the developed method can be considered feasible to be applied to MPH residual contamination analysis in sewage using a widely available apparatus.

Vivências de pessoas colostomizadas- Revisão Integrativa / Experiences of colostomided people- Integrative Review / Experiencias de personas colostomizadas- Revisión integrativa

Objetivo: Analisar a qualidade de vida em relação ao uso da bolsa coletora em pessoas ostomizadas. Método: Este estudo teve como base uma abordagem qualitativa e método de revisão bibliográfica integrativa. Resultados: No presente estudo, foram analisados 22 (vinte e dois) artigos que atenderam aos critérios de inclusão. Dos achados da pesquisa foram criadas 8 categorias. Conclusão: O estudo buscou uma maior conscientização sobre as necessidades das pessoas ostomizadas durante e após o uso da bolsa coletora, para que enfermeiros e familiares deem o suporte necessário, compreendendo os sentimentos que são despertados e como a situação afeta a vida de cada ostomizado.

Objective: To analyze the quality of life in relation to the use of the collection bag in ostomized people. Method: This study was based on a qualitative approach and an integrative literature review method. Results: In the present study, 22 (twenty-two) articles that met the inclusion criteria were analyzed. From the research findings, 8 categories were created.Conclusion: The study sought greater awareness of the needs of ostomates during and after the use of the collection bag, so that nurses and family members provide the necessary support, understanding the feelings that are aroused and how the situation affects the life of each ostomate.

Objetivo: Analizar la calidad de vida en relación al uso de la bolsa colectora en ostomizados. Método: Este estudio se basó en un enfoque cualitativo y un método integrador de revisión de la literatura. Resultados: En el presente estudio se analizaron 22 (veintidós) artículos que cumplieron con los criterios de inclusión. A partir de los resultados de la investigación, se crearon 8 categorías.Conclusión: El estudio buscó una mayor conciencia de las necesidades de los ostomizados durante y después del uso de la bolsa de recolección, para que los enfermeros y familiares brinden el apoyo necesario, comprendiendo los sentimientos que despiertan y cómo la situación afecta la vida de cada ostomizado

Comparação entre metodologias de simulação e ensino tradicional nas práticas de educação permanente com enfermeiros / Comparación entre metodologías de simulación y enseñanza tradicional en prácticas de educación permanente con enfermeras / Comparison between simulation methodologies and traditional teaching in permanent education practices with nurses

Objetivo: comparar os efeitos entre a metodologia de simulação e a aula tradicional na aquisição de conhecimentos de enfermeiros sobre a Necessidade Humana Básica de Oxigenação. Método: estudo transversal mediante atividades de Educação Permanente com metodologia de simulação e ensino tradicional com dois grupos de enfermeiros. Realizou-se quatro oficinas em três etapas: pré-teste; simulação ou aula expositiva; pós-teste. Definiu-se para normalidade dos dados o teste Kolmogorov Smirnov, para comparação das médias de acertos, os testes t de Student ou Wilcoxon, e nível de confiança de <0,05. Resultados: a amostra total alcançou maior média de acertos no pós-teste em todas as oficinas, sendo iguais nas de Tubo Orotraqueal e Traqueostomia. O primeiro grupo alcançou maior média de acertos nas oficinas sobre Oxigenoterapia e Oximetria, e o segundo, na oficina sobre Aspiração de Vias Aéreas. Conclusão: não se pôde inferir qual metodologia promoveu maior aquisição de conhecimento entre os grupos.

Objetivo: comparar los efectos entre la metodología de simulación y la clase tradicional en la adquisición de conocimientos de enfermería sobre la Necesidad Humana Básica de Oxigenación. Método: estudio transversal a través de actividades de Educación Permanente con metodología de simulación y enseñanza tradicional con dos grupos de enfermeras. Se celebraron cuatro talleres en tres etapas: pre-prueba; clase de simulación o exhibición; Prueba. La prueba de Kolmogorov Smirnov se definió para la normalidad de los datos, para la comparación de las medias de las respuestas correctas, las pruebas t de Student o Wilcoxon y el nivel de confianza de <0.05. Resultados: la muestra total alcanzó un mayor promedio de respuestas correctas en el post-test en todos los talleres, siendo la misma en los de Tubo Orotracqueal y Traqueostomía. El primer grupo alcanzó un promedio más alto de respuestas correctas en los talleres de Oxigenoterapia y Oximetría, y el segundo en el taller de Aspiración de Vía Aérea. Conclusión: no fue capaz de inferir qué metodología promovió una mayor adquisición de conocimientos entre los grupos.

Objective: to compare the effects between the simulation methodology and the traditional class in the acquisition of nurses' knowledge about the Basic Human Need for Oxygenation. Method: cross-sectional study through Permanent Education activities with simulation methodology and traditional teaching with two groups of nurses. Four workshops were held in three stages: pre-test; simulation or exhibition class; Test. The Kolmogorov Smirnov test was defined for normality of the data, for comparison of the means of correct answers, the Student or Wilcoxon t-tests, and the confidence level of <0.05. Results: the total sample reached a higher average of correct answers in the post-test in all workshops, being the same in those of Orotracheal Tube and Tracheostomy. The first group reached a higher average of correct answers in the workshops on Oxygen therapy and Oximetry, and the second in the workshop on Airway Aspiration. Conclusion: it was not able to infer which methodology promoted greater knowledge acquisition among the groups.

The de novo FAIRification process of a registry for vascular anomalies.

BACKGROUND: Patient data registries that are FAIR-Findable, Accessible, Interoperable, and Reusable for humans and computers-facilitate research across multiple resources. This is particularly relevant to rare diseases, where data often are scarce and scattered. Specific research questions can be asked across FAIR rare disease registries and other FAIR resources without physically combining the data. Further, FAIR implies well-defined, transparent access conditions, which supports making sensitive data as open as possible and as closed as necessary. RESULTS: We successfully developed and implemented a process of making a rare disease registry for vascular anomalies FAIR from its conception-de novo. Here, we describe the five phases of this process in detail: (i) pre-FAIRification, (ii) facilitating FAIRification, (iii) data collection, (iv) generating FAIR data in real-time, and (v) using FAIR data. This includes the creation of an electronic case report form and a semantic data model of the elements to be collected (in this case: the "Set of Common Data Elements for Rare Disease Registration" released by the European Commission), and the technical implementation of automatic, real-time data FAIRification in an Electronic Data Capture system. Further, we describe how we contribute to the four facets of FAIR, and how our FAIRification process can be reused by other registries. CONCLUSIONS: In conclusion, a detailed de novo FAIRification process of a registry for vascular anomalies is described. To a large extent, the process may be reused by other rare disease registries, and we envision this work to be a substantial contribution to an ecosystem of FAIR rare disease resources.

Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifers.

In cattle, uterine luminal fluid (ULF) is the main source of molecules that support embryo development and survival during the peri-implantation period. Our overarching hypothesis is that peri-estrus changes in uterine function, including ULF accumulation and absorption, are uneven among individuals, and affect ULF composition and fertility. Our objectives were (1) to characterize temporal and spatial changes in ULF volume, endometrial and luteal blood perfusion, endometrial and luteal size, and circulating progesterone concentrations during the peri-estrus period in beef heifers and (2) to associate such changes with the metabolite composition in the ULF, 4 days after estrus (d 0). Fourteen Bos indicus heifer that presented a PGF2α responsive CL received 500 µg PGF2α analog i.m. and were examined daily by rectal B-mode and pulse-wave color-Doppler ultrasonography until the fifth day after estrus (d 5). The composition of the ULF was analyzed by targeted mass spectrometry on d 4. Multivariate analyses clustered heifers according to ovarian, uterine, and hormonal variables in clusters A (n = 5) and B (n = 8 heifers). Concentrations of Pro, Ala, Leu, Gly, Val, Lys, Ile, Phe, Asp, Orn, Tyr, Arg, Trp, Suc, Cit, ADMA, the sum of essential Amino Acids (AA), sum of nonessential AA, sum of aromatic AA, and total AA were greater in cluster A (FDR ≤ 0.05). ULF volume dynamics and uterine, ovarian, and hormonal variables during the peri-estrus period presented a concerted variation among heifers within clusters, which was associated with the ULF composition 4 days after estrus.

De-novo FAIRification via an Electronic Data Capture system by automated transformation of filled electronic Case Report Forms into machine-readable data.

INTRODUCTION: Existing methods to make data Findable, Accessible, Interoperable, and Reusable (FAIR) are usually carried out in a post hoc manner: after the research project is conducted and data are collected. De-novo FAIRification, on the other hand, incorporates the FAIRification steps in the process of a research project. In medical research, data is often collected and stored via electronic Case Report Forms (eCRFs) in Electronic Data Capture (EDC) systems. By implementing a de novo FAIRification process in such a system, the reusability and, thus, scalability of FAIRification across research projects can be greatly improved. In this study, we developed and implemented a novel method for de novo FAIRification via an EDC system. We evaluated our method by applying it to the Registry of Vascular Anomalies (VASCA). METHODS: Our EDC and research project independent method ensures that eCRF data entered into an EDC system can be transformed into machine-readable, FAIR data using a semantic data model (a canonical representation of the data, based on ontology concepts and semantic web standards) and mappings from the model to questions on the eCRF. The FAIRified data are stored in a triple store and can, together with associated metadata, be accessed and queried through a FAIR Data Point. The method was implemented in Castor EDC, an EDC system, through a data transformation application. The FAIRness of the output of the method, the FAIRified data and metadata, was evaluated using the FAIR Evaluation Services. RESULTS: We successfully applied our FAIRification method to the VASCA registry. Data entered on eCRFs is automatically transformed into machine-readable data and can be accessed and queried using SPARQL queries in the FAIR Data Point. Twenty-one FAIR Evaluator tests pass and one test regarding the metadata persistence policy fails, since this policy is not in place yet. CONCLUSION: In this study, we developed a novel method for de novo FAIRification via an EDC system. Its application in the VASCA registry and the automated FAIR evaluation show that the method can be used to make clinical research data FAIR when they are entered in an eCRF without any intervention from data management and data entry personnel. Due to the generic approach and developed tooling, we believe that our method can be used in other registries and clinical trials as well.

Prevalence of human T-lymphotropic virus type 1 and 2 (HTLV-1/-2) infection in pregnant women in Brazil: a systematic review and meta-analysis.

Human T-lymphotropic virus type 1 (HTLV-1) infection may cause serious disease, while pathogenicity of HTLV-2 is less certain. There are no screening or surveillance programs for HTLV-1/-2 infection in Brazil. By performing this systematic review, we aimed to estimate the prevalence of HTLV-1/-2 infections in pregnant women in Brazil. This review included cohort and cross-sectional studies that assessed the presence of either HTLV-1/-2 infection in pregnant women in Brazil. We searched BVS/LILACS, Cochrane Library/CENTRAL, EMBASE, PubMed/MEDLINE, Scopus, Web of Science and gray literature from inception to August 2020. We identified 246 records in total. Twenty-six of those were included in the qualitative synthesis, while 17 of them were included in the meta-analysis. The prevalence of HTLV-1 in Brazilian pregnant women, as diagnosed by a positive screening test and a subsequent positive confirmatory test, was 0.32% (95% CI 0.19-1.54), while of HTLV-2 was 0.04% (95% CI 0.02-0.08). Subgroup analysis by region showed the highest prevalence in the Northeast region (0.60%; 95% CI 0.37-0.97) for HTLV-1 and in the South region (0.16%; 95% CI 0.02-1.10) for HTLV-2. The prevalence of HTLV-1 is much higher than HTLV-2 infection in pregnant Brazilian women with important differences between regions. The prevalence of both HTLV-1/-2 are higher in the Northeast compared to Center-West region.

Visual outcomes in advanced keratoconus using different strategies: Scleral lens, intracorneal ring segment and lamellar keratoplasty.

PURPOSE: To compare VA outcomes in patients with advanced keratoconus wearing SCL or after ICRS or DALK. In addition, determine the percentage of complimentary treatments after ICRS and DALK, and which of these was fitting a SCL, and final visual acuity (f-VA) results. METHODS: Retrospective case series analyzed 55 eyes with advanced keratoconus fitted with SCL, or after ICRS or a DALK between 2010 and 2018. The variables analyzed were sex, age, UDVA, CDVA, SEQ, K-readings, and f-VA after complimentary treatments. RESULTS: Twenty-eight eyes underwent a DALK, 14 were fitted with a SCL, and other 13 underwent ICRS insertion. Patients after DALK and after ICRS showed statistically significant improvement in UDVA and CDVA after surgery, with no difference between these groups. Patients fitted with SCL showed statistically higher CDVA improvement when compared to ICRS. After DALK and ICRS, respectively, nine eyes (32.14%) and seven eyes (53.85%) were fitted with SCL. Comparison between f-VA with SCL in three groups showed that the best result was achieved in DALK + SCL, with statistically significant difference to only SCL. CONCLUSION: We showed that the CDVA of eyes fitted with SCL without any surgical treatment was statistically better than CDVA of eyes after ICRS insertion. After DALK and ICRS insertion, many patients needed a complimentary treatment to improve CDVA. The most chosen treatment was fitting a SCL. After this, all eyes showed statistically significant improvement in f-VA, with statistically better results for DALK + SCL when compared to SCL fitted in advanced keratoconus without any surgical treatment.