RESUMEN
OBJECTIVE: Refractory status epilepticus is one of the most life-threatening neurological emergencies and is characterized by high morbidity and mortality. Additionally, the use of anti-inflammatory drugs during this period is very controversial. Thus, this study has been designed to analyze the effect of a low dose of indomethacin (a COX inhibitor) on the expression of inflammatory molecules. METHOD: The hippocampus of rats submitted to pilocarpine-induced long-lasting status epilepticus was analyzed to determine the expression of inflammatory molecules with RT-PCR and immunohistochemistry. RESULTS: Compared with controls, reduced levels of the kinin B2 receptors IL1ß and TNFα were found in the hippocampus of rats submitted to long-lasting status epilepticus and treated with indomethacin. CONCLUSIONS: These data show that low doses of indomethacin could be employed to minimize inflammation during long-lasting status epilepticus.
Asunto(s)
Inhibidores de la Ciclooxigenasa/farmacología , Hipocampo/efectos de los fármacos , Indometacina/farmacología , Monocinas/efectos de los fármacos , Receptores de Bradiquinina/efectos de los fármacos , Estado Epiléptico/tratamiento farmacológico , Animales , Modelos Animales de Enfermedad , Regulación hacia Abajo/efectos de los fármacos , Interleucina-1beta/análisis , Interleucina-1beta/efectos de los fármacos , Masculino , Monocinas/análisis , Pilocarpina , Ratas Wistar , Receptor de Bradiquinina B1/análisis , Receptor de Bradiquinina B1/efectos de los fármacos , Receptor de Bradiquinina B2/análisis , Receptor de Bradiquinina B2/efectos de los fármacos , Receptores de Bradiquinina/análisis , Estado Epiléptico/inducido químicamente , Factor de Necrosis Tumoral alfa/análisis , Factor de Necrosis Tumoral alfa/efectos de los fármacosRESUMEN
OBJECTIVE: Refractory status epilepticus is one of the most life-threatening neurological emergencies and is characterized by high morbidity and mortality. Additionally, the use of anti-inflammatory drugs during this period is very controversial. Thus, this study has been designed to analyze the effect of a low dose of indomethacin (a COX inhibitor) on the expression of inflammatory molecules. METHOD: The hippocampus of rats submitted to pilocarpine-induced long-lasting status epilepticus was analyzed to determine the expression of inflammatory molecules with RT-PCR and immunohistochemistry. RESULTS: Compared with controls, reduced levels of the kinin B2 receptors IL1β and TNFα were found in the hippocampus of rats submitted to long-lasting status epilepticus and treated with indomethacin. CONCLUSIONS: These data show that low doses of indomethacin could be employed to minimize inflammation during long-lasting status epilepticus. .
