Occurrence and distribution of Panstrongylus megistus (Burmeister, 1835) (Hemiptera, Reduviidae) in a metropolitan area of Southern Brazil.

Two triatomine genera (Panstrongylus and Triatoma) have essentially been recorded in Rio Grande do Sul State, Brazil. Panstrongylus megistus should be highlighted since this species is one of the main vectors of Trypanosoma cruzi in Brazil, due to its wide geographical distribution and the high susceptibility to this protozoan. This study aimed to present not only the occurrence and distribution of P. megistus in the Porto Alegre city's metropolitan area (PAMA), Rio Grande do Sul State, Brazil, but also the rates of T. cruzi infection, from 2009 to 2020. The PAMA, which comprises 34 cities and 4.4 million habitants, extends across the transition area in two biomes - Pampa and Mata Atlantica - found in the state. Results showed that P. megistus was recorded in 76.5% of cities (i.e., 26 out of 34), mainly in Porto Alegre city, where the vector was found in 11 out of the 12 years that were monitored. Three hundred and nineteen specimens were captured. Two hundred and sixty-seven specimens (83.7%) were located intradomicile (p < 0.0001), while 52.3% positivity for T. cruzi was found. Thus, P. megistus is important in the PAMA, because it invades and colonizes the households. Moreover, the high rates of T. cruzi infection have drawn attention.

Triatoma rubrovaria (Hemiptera, Reduviidae, Triatominae) in the Pampa biome, Brazil: a retrospective study of its occurrence and abundance.

Triatoma rubrovaria has been captured in some areas of Rio Grande do Sul State (RS), Brazil, as this species can be found in the Pampa biome. Its distribution across this biome should be described in detail to verify the potential of this vector to transmit Trypanosoma cruzi. This study aimed to investigate the occurrence of T. rubrovaria in the Pampa biome and transitional areas of RS. The collected information resulted from the analysis of secondary data provided by the Centro Estadual de Vigilancia em Saude (CEVS - State's Center of Health Surveillance). The following aspects were taken into consideration: the year in which the insect was captured, the city, the number of specimens captured, invasion or domiciliation, the notification in the household, surroundings or both, and T. cruzi infection. The data comprised the period from 2009 to 2020, in 109 cities located in the Pampa biome and 98 located in transitional areas. The Pampa biome exhibited 85% of the occurrences of T. rubrovaria, while 1.2% of specimens were T. cruzi-like positive. Both the first and second biennia concentrated 64.6% of captures. Alegrete city, Cangucu city and Piratini city were the locations in the Pampa where the largest numbers of specimens were found. Regarding the transitional areas, Roque Gonzales city, Santiago city and Santana da Boa Vista city exhibited the largest numbers. Most insects were adults, which were found in households. Even though positivity for T. cruzi-like was low, the species still has epidemiological importance in the region.

Occurrence and distribution of Panstrongylus megistus (Burmeister, 1835) (Hemiptera, Reduviidae) in a metropolitan area of Southern Brazil

ABSTRACT Two triatomine genera (Panstrongylus and Triatoma) have essentially been recorded in Rio Grande do Sul State, Brazil. Panstrongylus megistus should be highlighted since this species is one of the main vectors of Trypanosoma cruzi in Brazil, due to its wide geographical distribution and the high susceptibility to this protozoan. This study aimed to present not only the occurrence and distribution of P. megistus in the Porto Alegre city's metropolitan area (PAMA), Rio Grande do Sul State, Brazil, but also the rates of T. cruzi infection, from 2009 to 2020. The PAMA, which comprises 34 cities and 4.4 million habitants, extends across the transition area in two biomes - Pampa and Mata Atlantica - found in the state. Results showed that P. megistus was recorded in 76.5% of cities (i.e., 26 out of 34), mainly in Porto Alegre city, where the vector was found in 11 out of the 12 years that were monitored. Three hundred and nineteen specimens were captured. Two hundred and sixty-seven specimens (83.7%) were located intradomicile (p < 0.0001), while 52.3% positivity for T. cruzi was found. Thus, P. megistus is important in the PAMA, because it invades and colonizes the households. Moreover, the high rates of T. cruzi infection have drawn attention.

Triatoma rubrovaria (Hemiptera, Reduviidae, Triatominae) in the Pampa biome, Brazil: a retrospective study of its occurrence and abundance

ABSTRACT Triatoma rubrovaria has been captured in some areas of Rio Grande do Sul State (RS), Brazil, as this species can be found in the Pampa biome. Its distribution across this biome should be described in detail to verify the potential of this vector to transmit Trypanosoma cruzi. This study aimed to investigate the occurrence of T. rubrovaria in the Pampa biome and transitional areas of RS. The collected information resulted from the analysis of secondary data provided by the Centro Estadual de Vigilancia em Saude (CEVS - State's Center of Health Surveillance). The following aspects were taken into consideration: the year in which the insect was captured, the city, the number of specimens captured, invasion or domiciliation, the notification in the household, surroundings or both, and T. cruzi infection. The data comprised the period from 2009 to 2020, in 109 cities located in the Pampa biome and 98 located in transitional areas. The Pampa biome exhibited 85% of the occurrences of T. rubrovaria, while 1.2% of specimens were T. cruzi-like positive. Both the first and second biennia concentrated 64.6% of captures. Alegrete city, Cangucu city and Piratini city were the locations in the Pampa where the largest numbers of specimens were found. Regarding the transitional areas, Roque Gonzales city, Santiago city and Santana da Boa Vista city exhibited the largest numbers. Most insects were adults, which were found in households. Even though positivity for T. cruzi-like was low, the species still has epidemiological importance in the region.

Seroprevalence of Trypanosoma cruzi infection in blood donors in the extreme South of Brazil.

BACKGROUND: We aimed to evaluate the seroprevalence of Chagas disease among blood donors in southern Rio Grande do Sul, Brazil. METHODS: The study was conducted from 2010 to 2019 based on data registered by the Hemocentro Regional de Pelotas, Rio Grande do Sul. RESULTS: There were 106,320 blood donations, and the discard rate of blood bags, either due to positive reactions to anti-T. cruzi antibodies or inconclusive results was 0.27% (283 bags). CONCLUSIONS: The usage of methods that enable the safe identification of donors with positive serology for Chagas disease is fundamental to ensure transfusional safety.

Soil contamination by Ancylostoma spp. and Toxocara spp. eggs in elementary school playgrounds in the extreme south of Brazil.

Millions of people worldwide, and especially schoolchildren, may be infected by geohelminths due to their exposure to a contaminated environment. The aim of this study was to evaluate soil contamination by Ancylostoma spp. and Toxocara spp. eggs in recreation areas at elementary schools in Pelotas, state of Rio Grande do Sul, Brazil. Sand samples were collected from 22 schools and were processed using the centrifugal flotation method. Helminth eggs with zoonotic potential were found in 12 out of the 22 schools (54.5%). Contamination by Ancylostoma spp. and Toxocara spp. was observed in 36.4% (8/22) and 27.3% (6/22) of the soil samples collected at these schools, respectively. These findings of eggs show that the school communities are exposed to risks of zoonotic transmission.

Seroprevalence of Trypanosoma cruzi infection in blood donors in the extreme South of Brazil

ABSTRACT Background: We aimed to evaluate the seroprevalence of Chagas disease among blood donors in southern Rio Grande do Sul, Brazil. Methods: The study was conducted from 2010 to 2019 based on data registered by the Hemocentro Regional de Pelotas, Rio Grande do Sul. Results: There were 106,320 blood donations, and the discard rate of blood bags, either due to positive reactions to anti-T. cruzi antibodies or inconclusive results was 0.27% (283 bags). Conclusions: The usage of methods that enable the safe identification of donors with positive serology for Chagas disease is fundamental to ensure transfusional safety.

Soil contamination by Ancylostoma spp. and Toxocara spp. eggs in elementary school playgrounds in the extreme south of Brazil / Contaminação do solo de praças de recreação de escolas de ensino fundamental por ovos de Ancylostoma spp. e Toxocara spp. no extremo sul do Brasil

Abstract Millions of people worldwide, and especially schoolchildren, may be infected by geohelminths due to their exposure to a contaminated environment. The aim of this study was to evaluate soil contamination by Ancylostoma spp. and Toxocara spp. eggs in recreation areas at elementary schools in Pelotas, state of Rio Grande do Sul, Brazil. Sand samples were collected from 22 schools and were processed using the centrifugal flotation method. Helminth eggs with zoonotic potential were found in 12 out of the 22 schools (54.5%). Contamination by Ancylostoma spp. and Toxocara spp. was observed in 36.4% (8/22) and 27.3% (6/22) of the soil samples collected at these schools, respectively. These findings of eggs show that the school communities are exposed to risks of zoonotic transmission.

Resumo Milhões de pessoas podem ser acometidas por geohelmintos, especialmente crianças em idade escolar, devido a sua maior exposição a ambientes contaminados. O objetivo deste estudo foi avaliar a contaminação do solo por ovos de Ancylostoma spp. e Toxocara spp. em áreas de recreação de escolas de ensino fundamental da cidade de Pelotas, Rio Grande do Sul, Brasil. Foram colhidas amostras de areia de 22 escolas e processadas pelo método de centrífugo-flutuação. Em 54,5% (12/22) das escolas houve registro da presença de ovos de helmintos com potencial zoonótico. A contaminação por Ancylostoma spp. e Toxocara spp. foi observada em 36,4% (8/22) e 27,3% (6/22) das amostras de solo das escolas, respectivamente. Existe a presença de ovos de Ancylostoma spp. e Toxocara spp., havendo risco de transmissão de zoonoses à comunidade escolar.

Current situation of Chagas disease vectors (Hemiptera, Reduviidae) in Southern Rio Grande do Sul State, Brazil.

Chagas disease (CD) has been considered endemic in the South of Rio Grande do Sul (RS) State, Brazil. This study aimed at evaluating the occurrence of Trypanosoma cruzi vectors based on the main species captured in Southern Rio Grande do Sul State from 2008 to 2019. The study area comprised counties that belong to the 3rd Regional Health Coordination (RHC) and to the 7th RHC, whose headquarters are in Pelotas and Bage, respectively. The study was based on secondary data provided by the partnership between the Federal University of Pelotas, Rio Grande do Sul State (UFPel-RS) and the State Health Surveillance Center in RS (SCHS-RS). One thousand and four hundred triatomines were captured in the area supervised by the 3rd RHC, mainly in Cangucu (37.7%), Piratini (22.4%) and Pinheiro Machado (15.1%), while, in the area supervised by the 7th RHC, the largest number of triatomines was captured in Lavras do Sul (64.15%). In both areas, Triatoma rubrovaria (90.6%) and Panstrongylus tupynambai (7.4%) were the most common species. Most were captured inside households but T. cruzi-positive insects were not found in the period under study. The results of this study show that, in Southern Rio Grande do Sul State, there is still a high rate of triatomine household invasion and dispersal, mainly by T. rubrovaria. Thus, the entomological surveillance should be maintained with the participation of the population and further studies should be deepened in the area.

Residual foci of Triatoma infestans infestation: Surveillance and control in Rio Grande do Sul, Brazil, 2001-2018.

INTRODUCTION: This retrospective study conducted from 2001 to 2018 investigated the residual foci of Triatoma infestans infestation in Rio Grande do Sul, Brazil. METHODS: The data were obtained via entomological surveillance and the distribution of vector occurrence. The coverage of active research was mapped. RESULTS: The largest coverage rate for active research was observed in the northwest region of the total of 515,081 domiciles researched. Most T. infestans specimens were captured in the peridomicile. CONCLUSIONS: Infestation has decreased significantly since 2008, and T. infestans has not been captured since 2015.

Residual foci of Triatoma infestans infestation: Surveillance and control in Rio Grande do Sul, Brazil, 2001-2018

Abstract INTRODUCTION: This retrospective study conducted from 2001 to 2018 investigated the residual foci of Triatoma infestans infestation in Rio Grande do Sul, Brazil. METHODS: The data were obtained via entomological surveillance and the distribution of vector occurrence. The coverage of active research was mapped. RESULTS: The largest coverage rate for active research was observed in the northwest region of the total of 515,081 domiciles researched. Most T. infestans specimens were captured in the peridomicile. CONCLUSIONS: Infestation has decreased significantly since 2008, and T. infestans has not been captured since 2015.

Helminth eggs with zoonotic potential in the vicinity of public schools in southern Brazil.

The aim of this study was to evaluate environmental contamination by helminth eggs with zoonotic potential that were found in dog feces in the vicinity of elementary schools. Seventy-nine samples of dog feces were collected from 28 municipal schools located in five neighborhoods in Pelotas, Rio Grande do Sul (RS), Brazil. The samples were processed using the Willis-Mollay technique and analyzed using an optical microscope (40X), to identify any parasite eggs present. All neighborhoods were positive and 74.7% of the samples exhibited one or more helminth genera. The agent with the highest prevalence was Ancylostoma spp. (93.2%), followed by Trichuris spp. (18.6%), Toxocara spp. (11.9%) and Toxascaris (1.7%). These data show that there is a need for greater care towards controlling these helminths with zoonotic potential, including responsible pet ownership and daily activities to clean and collect dog feces in the vicinity of schools, because these are places where children play and study.

Helminth eggs with zoonotic potential in the vicinity of public schools in southern Brazil / Ovos de helmintos com potencial zoonótico nas proximidades de escolas públicas no extremo sul do Brasil

The aim of this study was to evaluate environmental contamination by helminth eggs with zoonotic potential that were found in dog feces in the vicinity of elementary schools. Seventy-nine samples of dog feces were collected from 28 municipal schools located in five neighborhoods in Pelotas, Rio Grande do Sul (RS), Brazil. The samples were processed using the Willis-Mollay technique and analyzed using an optical microscope (40X), to identify any parasite eggs present. All neighborhoods were positive and 74.7% of the samples exhibited one or more helminth genera. The agent with the highest prevalence was Ancylostoma spp. (93.2%), followed by Trichuris spp. (18.6%), Toxocara spp. (11.9%) and Toxascaris (1.7%). These data show that there is a need for greater care towards controlling these helminths with zoonotic potential, including responsible pet ownership and daily activities to clean and collect dog feces in the vicinity of schools, because these are places where children play and study.(AU)

O objetivo deste estudo foi avaliar a contaminação ambiental por ovos de helmintos com potencial zoonótico, diagnosticados em fezes caninas depositadas nas proximidades de escolas primárias. Setenta e nove amostras de fezes foram colhidas em 28 escolas municipais localizadas em cinco bairros da cidade de Pelotas, Rio Grande do Sul (RS), Brasil. As amostras foram processadas pela técnica de Willis-Mollay e analisadas em microscópio óptico (40X), para identificar quaisquer parasitos presentes. Todos os bairros foram positivos e 74,7% das amostras apresentaram um ou mais gêneros de helmintos. O agente com maior prevalência foi Ancylostoma spp. (93,2%), seguido por Trichuris spp. (18,6%), Toxocara spp. (11,9%) e Toxascaris (1,7%). Esses dados mostram que há necessidade de maior cuidado no controle desses helmintos com potencial zoonótico, incluindo a posse responsável dos animais e atividades diárias de limpeza e colheita de fezes de cães nas proximidades das escolas, porque esses são locais onde as crianças brincam e estudam.(AU)

Helminth eggs with zoonotic potential in the vicinity of public schools in southern Brazil / Ovos de helmintos com potencial zoonótico nas proximidades de escolas públicas no extremo sul do Brasil

Abstract The aim of this study was to evaluate environmental contamination by helminth eggs with zoonotic potential that were found in dog feces in the vicinity of elementary schools. Seventy-nine samples of dog feces were collected from 28 municipal schools located in five neighborhoods in Pelotas, Rio Grande do Sul (RS), Brazil. The samples were processed using the Willis-Mollay technique and analyzed using an optical microscope (40X), to identify any parasite eggs present. All neighborhoods were positive and 74.7% of the samples exhibited one or more helminth genera. The agent with the highest prevalence was Ancylostoma spp. (93.2%), followed by Trichuris spp. (18.6%), Toxocara spp. (11.9%) and Toxascaris (1.7%). These data show that there is a need for greater care towards controlling these helminths with zoonotic potential, including responsible pet ownership and daily activities to clean and collect dog feces in the vicinity of schools, because these are places where children play and study.

Resumo O objetivo deste estudo foi avaliar a contaminação ambiental por ovos de helmintos com potencial zoonótico, diagnosticados em fezes caninas depositadas nas proximidades de escolas primárias. Setenta e nove amostras de fezes foram colhidas em 28 escolas municipais localizadas em cinco bairros da cidade de Pelotas, Rio Grande do Sul (RS), Brasil. As amostras foram processadas pela técnica de Willis-Mollay e analisadas em microscópio óptico (40X), para identificar quaisquer parasitos presentes. Todos os bairros foram positivos e 74,7% das amostras apresentaram um ou mais gêneros de helmintos. O agente com maior prevalência foi Ancylostoma spp. (93,2%), seguido por Trichuris spp. (18,6%), Toxocara spp. (11,9%) e Toxascaris (1,7%). Esses dados mostram que há necessidade de maior cuidado no controle desses helmintos com potencial zoonótico, incluindo a posse responsável dos animais e atividades diárias de limpeza e colheita de fezes de cães nas proximidades das escolas, porque esses são locais onde as crianças brincam e estudam.

Seroprevalence of anti-TRYPANOSOMA CRUZI and anti-TOXOPLASMA GONDII antibodies in possible and potential organ donors in the south of Rio Grande do Sul state, Brazil

Serological profiles are important in cases of solid organ donation where serological findings, such as antibodies against Trypanosoma cruzi and toxoplasmosis, may interfere in organ donation by increasing morbidity and mortality. This study aimed to outline seroprevalence of anti-Trypanosoma cruzi and anti-Toxoplasma gondii antibodies in possible and potential organ donors in the south of Rio Grande do Sul (RS) State, Brazil. A cross-sectional quantitative and retrospective epidemiological study was carried out, based on secondary data. Data on serology were extracted from medical records found in the Sistema de Gestão Hospitalar (SIGH), a hospital management system used by a university hospital in the south of RS, Brazil. Sociodemographic variables, such as age, sex and race, as well as clinical variables, such as titration of IgM and IgG anti-T. cruzi and anti­T. gondii antibodies, were analyzed. Medical records were found to be poorly filled in, since 67.6% (506 records) could not be used due to lack of information. Seroprevalence of T. cruzi was 6.8% (5 cases), mostly in white males. Regarding seroprevalence of T. gondii, 76.2% (64 donors) were serologicaly positive, 10.9% of which were IgM positive. This is the first study on anti-T. cruzi and anti-T. gondii antibodies in organ donors in RS, Brazil and should be furthered since there may be consequences regarding organ uptake and donation.

Ovicidal and larvicidal potential of Rosmarinus officinalis to control gastrointestinal nematodes of sheep / Potencial ovicida e larvicida de Rosmarinus officinalis para controle de nematódeos gastrintestinais de ovinos

Abstract Gastrointestinal Nematode Infection (GIN) are the main constraint to the production of small ruminants. Studies of medicinal plants have been an important alternative in the effort to control these parasites. Therefore, the purpose of this study was to evaluate the in vitro ovicidal and larvicidal activity of essential oil of Rosmarinus officinalis. The oil was extracted, analyzed by gas chromatography and tested on GIN eggs and larvae in six concentrations, 227.5mg/mL, 113.7mg/mL, 56.8mg/mL, 28.4mg/mL, 14.2mg/mL and 7.1mg/mL. To determine the ovicidal activity, GIN eggs were recovered from sheep feces and incubated for 48h with different concentrations of the oil. For the evaluation of larval migration, third-stage larvae (L3) were obtained by fecal culture, and associated with the essential oil for 24h at the same concentrations, after which they were left for another 24 hours on microsieves, followed by the count of migrating and non-migrating larvae. The assays of R. officinalis oil showed a significant (p<0.05) 97.4% to 100% inhibition of egg hatching and a significant (p<0.05) 20% to 74% inhibition of larval migration. The main constituent revealed by gas chromatography was Eucalyptol. The results indicate that R. officinalis essential oil has ovicidal and larvicidal activity on sheep GINs.

Resumo As infecções por nematódeos gastrintestinais (ING) constituem a maior limitação à produção de pequenos ruminantes. Na busca do controle desses parasitos, estudos com plantas medicinais têm sido uma importante alternativa. Visto isto, o estudo desenvolvido teve como objetivo avaliar a ação ovicida e larvicida in vitro do óleo essencial de Rosmarinus officinalis. O óleo foi extraído, analisado por cromatografia gasosa e testado sobre ovos e larvas de ING em seis concentrações, 227,5mg/mL; 113,7mg/mL; 56,8mg/mL; 28,4mg/mL; 14,2mg/mL; 7,1mg/mL. Para determinar a ação ovicida, ovos de ING foram recuperados de fezes de ovinos e incubados por 48h com as diferentes concentrações do óleo. Na avaliação da migração das larvas, as larvas de terceiro estágio (L3) foram obtidas por coprocultura, e associadas ao óleo essencial por 24h nas mesmas concentrações, permanecendo por mais 24h em microtamises, seguindo-se a contagem de larvas que migraram e que não migraram. Os testes in vitro com o óleo de R. officinalis mostraram o nível de significância (p<0.05) 97,4% a 100% na inibição da eclodibilidade e 20% a 74% na inibição da migração das larvas. Na análise por cromatografia gasosa o constituinte majoritário foi o eucaliptol. Os resultados apresentados mostram que o óleo essencial de R. officinalis possui ação ovicida e larvicida sobre ING de ovinos.

Ovicidal and larvicidal potential of Rosmarinus officinalis to control gastrointestinal nematodes of sheep.

Gastrointestinal Nematode Infection (GIN) are the main constraint to the production of small ruminants. Studies of medicinal plants have been an important alternative in the effort to control these parasites. Therefore, the purpose of this study was to evaluate the in vitro ovicidal and larvicidal activity of essential oil of Rosmarinus officinalis. The oil was extracted, analyzed by gas chromatography and tested on GIN eggs and larvae in six concentrations, 227.5mg/mL, 113.7mg/mL, 56.8mg/mL, 28.4mg/mL, 14.2mg/mL and 7.1mg/mL. To determine the ovicidal activity, GIN eggs were recovered from sheep feces and incubated for 48h with different concentrations of the oil. For the evaluation of larval migration, third-stage larvae (L3) were obtained by fecal culture, and associated with the essential oil for 24h at the same concentrations, after which they were left for another 24 hours on microsieves, followed by the count of migrating and non-migrating larvae. The assays of R. officinalis oil showed a significant (p<0.05) 97.4% to 100% inhibition of egg hatching and a significant (p<0.05) 20% to 74% inhibition of larval migration. The main constituent revealed by gas chromatography was Eucalyptol. The results indicate that R. officinalis essential oil has ovicidal and larvicidal activity on sheep GINs.

Ovicidal and larvicidal potential of Rosmarinus officinalis to control gastrointestinal nematodes of sheep / Potencial ovicida e larvicida de Rosmarinus officinalis para controle de nematódeos gastrintestinais de ovinos

Gastrointestinal Nematode Infection (GIN) are the main constraint to the production of small ruminants. Studies of medicinal plants have been an important alternative in the effort to control these parasites. Therefore, the purpose of this study was to evaluate the in vitro ovicidal and larvicidal activity of essential oil of Rosmarinus officinalis. The oil was extracted, analyzed by gas chromatography and tested on GIN eggs and larvae in six concentrations, 227.5mg/mL, 113.7mg/mL, 56.8mg/mL, 28.4mg/mL, 14.2mg/mL and 7.1mg/mL. To determine the ovicidal activity, GIN eggs were recovered from sheep feces and incubated for 48h with different concentrations of the oil. For the evaluation of larval migration, third-stage larvae (L3) were obtained by fecal culture, and associated with the essential oil for 24h at the same concentrations, after which they were left for another 24 hours on microsieves, followed by the count of migrating and non-migrating larvae. The assays of R. officinalis oil showed a significant (p 0.05) 97.4% to 100% inhibition of egg hatching and a significant (p 0.05) 20% to 74% inhibition of larval migration. The main constituent revealed by gas chromatography was Eucalyptol. The results indicate that R. officinalis essential oil has ovicidal and larvicidal activity on sheep GINs.(AU)

As infecções por nematódeos gastrintestinais (ING) constituem a maior limitação à produção de pequenos ruminantes. Na busca do controle desses parasitos, estudos com plantas medicinais têm sido uma importante alternativa. Visto isto, o estudo desenvolvido teve como objetivo avaliar a ação ovicida e larvicida in vitro do óleo essencial de Rosmarinus officinalis. O óleo foi extraído, analisado por cromatografia gasosa e testado sobre ovos e larvas de ING em seis concentrações, 227,5mg/mL; 113,7mg/mL; 56,8mg/mL; 28,4mg/mL; 14,2mg/mL; 7,1mg/mL. Para determinar a ação ovicida, ovos de ING foram recuperados de fezes de ovinos e incubados por 48h com as diferentes concentrações do óleo. Na avaliação da migração das larvas, as larvas de terceiro estágio (L3) foram obtidas por coprocultura, e associadas ao óleo essencial por 24h nas mesmas concentrações, permanecendo por mais 24h em microtamises, seguindo-se a contagem de larvas que migraram e que não migraram. Os testes in vitro com o óleo de R. officinalis mostraram o nível de significância (p 0.05) 97,4% a 100% na inibição da eclodibilidade e 20% a 74% na inibição da migração das larvas. Na análise por cromatografia gasosa o constituinte majoritário foi o eucaliptol. Os resultados apresentados mostram que o óleo essencial de R. officinalis possui ação ovicida e larvicida sobre ING de ovinos.(AU)

Seroprevalence of Toxoplasma gondii and Toxocara canis in a human rural population of Southern Rio Grande do Sul.

Due to the growing population of pets, especially homeless dogs and cats, zoonoses still represent a significant public health problem. Toxoplasma gondii and Toxocara spp. are epidemiologically important zoonotic agents as they are etiological factors of human toxoplasmosis and toxocariasis, respectively. These parasites remain neglected even though they are substantially prevalent in rural areas. The aim of this study was to investigate T. gondii and T. canis seroprevalence and risk factors of seropositivity in a rural population in Pelotas municipality, Brazil. The study participants (n=344) were patients of a Basic Healthcare Unit (BHU) located in Cerrito Alegre. Blood samples were collected and tested for T. gondii antibodies by indirect immunofluorescence and T. canis antibodies by an indirect ELISA that targets an excreted-secreted antigen (TES). T. gondii seropositivity was 53.2%, with higher titers (1:256 - 1:1,024) in individuals who habitually eat pork, beef, or chicken, while T. canis seropositivity was 71.8% and concomitant T. gondii and T. canis seropositivity was 38.3%. Among the seropositivity risk factors assessed, only habitual undercooked meat consumption was significant (p = 0.046; OR = 3.7) for T. gondii and none of them were associated with T. canis seropositivity. Both parasites have a high prevalence in rural areas, which reinforces the need to invest in rural community education and health.

Health education in chagas disease control: making an educational video

Several studies have shown that the population has relatively little information regarding Chagas disease (CD) and its vectors; however, this knowledge is relevant because community participation is vital for success in disease control actions. For this reason, and due to the lack of audiovisual material on this subject in the country, this study focused on making an educational documentary on CD and its vectors, which could be available to the population for free. The video preparation was divided into three phases: Pre-production, Production and Post-production. The site chosen for shooting was northwestern Rio Grande do Sul State due to the Triatoma infestans vector persistence in recent decades in that area. A documentary was obtained which addresses relevant CD aspects, such as its history, transmission, major vectors and biology, phases of the disease and, in particular, how to inspect the intra-domiciliary and peri-domiciliary areas in search of triatomine bugs or "kissing bugs". The use of videos as an educational tool helps broadcast information; therefore, this documentary is a public use tool, which aims to promote the control and prophylaxis of CD and its vectors.