Inoculation with Azospirillum brasilense Strains AbV5 and AbV6 Increases Nutrition, Chlorophyll, and Leaf Yield of Hydroponic Lettuce.

Inoculation with Azospirillum brasilense has promisingly increased plant yield and nutrient acquisition. The study aimed to estimate the dose of A. brasilense that increases yield, gas exchange, nutrition, and foliar nitrate reduction. The research was carried out in a greenhouse at Ilha Solteira, in a hydroponic system in randomized blocks with four replicates. The treatments consisted of doses of inoculation with A. brasilense strains AbV5 and AbV6 via nutrient solution (0, 8, 16, 32, and 64 mL 100 L-1). Inoculation with A. brasilense at calculated doses between 20 and 44 mL provided the highest fresh and dry mass of shoots and roots, number of leaves, and leaf yield. In addition, the calculated doses of inoculation with A. brasilense increased the accumulation of N, P, K, Ca, Mg, S, B, Fe, Mn, and Zn in shoots and roots, except the accumulation of Ca in roots. It also increased cell membrane integrity index (15%), relative water content (13%), net photosynthesis rate (85%), intracellular CO2 concentration (15%), total chlorophyll (46%), stomatal conductance (56%), transpiration (15%), and water use efficiency (59%). Hence, inoculation with A. brasilense at doses between 20 and 44 mL 100 L-1 is considered the best approach for increasing the growth, yield, accumulation of nutrients, and gas exchange of hydroponically grown iceberg lettuce.

Inoculation with Trichoderma harzianum and Azospirillum brasilense increases nutrition and yield of hydroponic lettuce.

The use of beneficial fungi and bacteria stimulate plant growth and serve to improve yield and food quality in a sustainable manner. The electrical conductivity of nutrients solution is closely linked to better nutrition of vegetable plants in a hydroponic system. Therefore, objectives of current study were to evaluate the effect of isolated and combined inoculation with Azospirillum brasilense and Trichoderma harzianum under two electrical conductivities on growth, nutrition, and yield of lettuce in hydroponic cultivation. The experiment was designed in a strip-plot block with five replications in a 4 × 2 factorial scheme. The treatments were consisted of four microbial inoculations (without, A. brasilense, T. harzianum and co-inoculation) and electrical conductivities (1.2 and 1.4 dS m-1). Inoculation with A. brasilense and T. harzianum increased lettuce root growth by 47% and 20%, respectively. The single inoculation of T. harzianum provided higher fresh leaves yield (24%) at electrical conductivity of 1.2 dS m-1, while single inoculation with A. brasilense increased fresh leaves yield by 17% at electrical conductivity 1.4 dS m-1. The lowest shoot NO3- accumulation (40%) was observed with inoculation of A. brasilense and highest (28%) with inoculation T. harzianum in both electrical conductivities. Inoculation with A. brasilense increased leaf accumulation of K, P, Ca, Mg, Fe, Mn, Cu, and Zn, which are essential for human nutrition and being recommended to improve yield of lettuce plants in hydroponics. It is recommended to use EC 1.4 dS m-1 of the nutrients solution to improve accumulation of K, Mn, Cu, and Zn, regardless of inoculations for biofortification of lettuce with application of fertilizers.