RESUMEN
We studied the influence of hypoxic-hypercapnic environment under the effect of hypothermia (artificial hibernation) on fatty acids spectrum of inner mitochondrial membrane (IMM) lipids of rat cardiomyocytes and hepatocytes. Specific for cellular organelles redistribution of IMM fatty acids was determined. It led to the reduction of total amount of saturated fatty acids (SFAs) and increase of unsaturated fatty acids (UFAs) in cardiomyocytes and to the increase of SFAs and decrease of UFAs in hepatocytes. The decrease in the content of oleic acid and increased content of arachidonic and docosahexaenoic acids in IMM were shown. This may be due to their role in the regulatory systems during hibernation, as well as following exit therefrom. It is assumed that artificial hibernation state is characterized by the stress reaction leading to optimal readjustment of fatty acids composition of membrane lipids, which supports functional activity of mitochondria in hepatocytes and cardiomyocytes.
Asunto(s)
Ácidos Grasos Insaturados/química , Ácidos Grasos/química , Hepatocitos/metabolismo , Hipercapnia/metabolismo , Hipoxia/metabolismo , Membranas Mitocondriales/metabolismo , Miocitos Cardíacos/metabolismo , Animales , Animales no Consanguíneos , Ácidos Grasos/clasificación , Ácidos Grasos/aislamiento & purificación , Ácidos Grasos Insaturados/clasificación , Ácidos Grasos Insaturados/aislamiento & purificación , Hibernación , Masculino , Lípidos de la Membrana/química , Lípidos de la Membrana/clasificación , Lípidos de la Membrana/aislamiento & purificación , Mitocondrias/química , Mitocondrias/metabolismo , Membranas Mitocondriales/química , Ratas , Estrés FisiológicoRESUMEN
We investigated the energy activity of mitochondria from rat cardiomyocytes under the artificial carbon dioxide hypobiosis, which led to physiological changes in the organism (the decrease of body temperature, the reduction of heart rate, etc.). The respiratory and phosphorylation activities in mitochondria of cardiomyocytes is reduced when using two oxidation substrates (succinate and malate), which characterize the rate of the oxygen consumption by the mitochondria. The partial uncoupling of the oxidation and phosphorylation processes when using the malate unlike succinate was established. The activity of NADH-KoQ-oxidoreductase (complex I of the respiratory chain) is inhibited, but the activities of succinate dehydrogenase and cytochrome oxidase don't change. Probably, the priority of the succinate use under the artificial hypobiosis provides the support of the mitochondria functional activity on a sufficient energy level. It is evidenced by the ATP-synthetase activity. The modifications of the structural and functional state of the inner mitochondria membrane of the cardiomyocytes are directed to the adaptation under the artificial carbon dioxide hypobiosis.
Asunto(s)
Dióxido de Carbono/farmacología , Mitocondrias Cardíacas/efectos de los fármacos , Membranas Mitocondriales/efectos de los fármacos , Fosforilación Oxidativa/efectos de los fármacos , Consumo de Oxígeno/efectos de los fármacos , Oxígeno/farmacología , Animales , Animales no Consanguíneos , Temperatura Corporal/efectos de los fármacos , Fraccionamiento Celular , Transporte de Electrón/efectos de los fármacos , Transporte de Electrón/fisiología , Complejo I de Transporte de Electrón/metabolismo , Complejo IV de Transporte de Electrones/metabolismo , Frecuencia Cardíaca/efectos de los fármacos , Hipotermia/metabolismo , Hipotermia/fisiopatología , Hipoxia/metabolismo , Hipoxia/fisiopatología , Malatos/metabolismo , Masculino , Mitocondrias Cardíacas/metabolismo , Membranas Mitocondriales/metabolismo , ATPasas de Translocación de Protón Mitocondriales/metabolismo , Miocitos Cardíacos/citología , Miocitos Cardíacos/efectos de los fármacos , Miocitos Cardíacos/metabolismo , Ratas , Succinato Deshidrogenasa/metabolismo , Ácido Succínico/metabolismoRESUMEN
Peculiarities of neutral amino acid (L-leucine and L-phenylalanine) transport in brush-border membranes of rat small intestine enterocytes under normal conditions and following a 1.0-Gy X-ray irradiation have been studied. The increase of the brush-border membrane permeability for ions and amino acids is considered to be the main reason of the post-irradiation disorders in the transmembrane transport of amino acids. The radiobiological approach made it possible to corroborate the existence of a Na+-dependent L-phenylalanine transport system different from the common system for neutral amino acids.
